In vitro and in vivo efficacy of carvacrol against Echinococcus granulosus.


:Currently, benzimidazoles are used as chemotherapeutic agents and as a complement to surgery and PAIR in the treatment of cystic echinococcosis (CE). They are generally applied at high doses causing side effects and, 50% of cases do not respond favorably to such chemotherapy. The use of essential oils obtained by distillation from aromatic plants would be an effective alternative or complementary to the synthetic compounds, because would not bring the appearance of side effects. Carvacrol and his isomer thymol are the main phenolic components from essential oils of Origanum vulgare (oregano) and Thymus vulgaris (thyme). The aim of the present work was to evaluate the in vitro and in vivo efficacy of carvacrol against Echinococcus granulosus metacestodes. For the in vitro assay, protoscoleces and cysts of E. granulosus were incubated with carvacrol at the following final concentrations: 10, 5 and 1μg/ml of carvacrol. The maximum protoscolicidal effect was found with 10μg/ml of carvacrol. Results of viability tests were consistent with the structural and ultrastructural damage observed in protoscoleces. Ultrastructural studies revealed that the germinal layer of cysts treated with carvacrol lost the multicellular structure feature. In the clinical efficacy study, a reduction in cyst weight was observed after the administration of 40mg/kg of carvacrol during 20days in mice with cysts developed during 4 months, compared to that of those collected from control mice. Given that the in vivo effect of carvacrol was comparable with the treatment of reference with ABZ and the fact that is a safe compound, we postulated that carvacrol may be an alternative option for treatment of human CE.


Acta Trop


Acta tropica


Fabbri J,Maggiore MA,Pensel PE,Denegri GM,Gende LB,Elissondo MC




Has Abstract


2016-12-01 00:00:00












  • Neuroinvasion by Chandipura virus.

    abstract::Chandipura virus (CHPV) is an arthropod borne rhabdovirus associated with acute encephalitis in children below the age of 15 years in the tropical states of India. Although the entry of the virus into the nervous system is among the crucial events in the pathogenesis of CHPV, the exact mechanism allowing CHPV to invad...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审


    authors: Rajasekharan S,Rana J,Gulati S,Gupta V,Gupta S

    更新日期:2014-07-01 00:00:00

  • Development of loop-mediated isothermal amplification (LAMP) for detection of Theileria equi.

    abstract::Several approaches have been developed for diagnosis of Theileria equi infection in horses and donkeys but all of them have limitations in practice. Due to numerous strengths including easy operation, cheapness and high sensitivity and specificity, LAMP has been already extensively used for surveillance of a number of...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Xie J,Liu G,Tian Z,Luo J

    更新日期:2013-09-01 00:00:00

  • Developing health research capability in Tanzania: from a Swiss Tropical Institute Field Laboratory to the Ifakara Centre of the Tanzanian National Institute of Medical Research.

    abstract::The paper summarizes the evolution of a biomedical field research station in Tanzania, established by a European institute, into a national health research and resource centre. The Swiss Tropical Institute Field Laboratory was founded in Ifakara in the Kilombero District in southeastern Tanzania in 1957. It has evolve...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Tanner M,Kitua A,Degrémont AA

    更新日期:1994-08-01 00:00:00

  • Diurnal periodicity of Leishmania amastigotes in peripheral blood of Indian Kala-azar patients.

    abstract::Peripheral blood smears of 20 patients with Indian Kala-azar (KA) were collected throughout the day and night and examined for presence of Leishmania amastigotes. Amastigotes were detected in 46% of peripheral blood samples taken during the day (08:00-17:00 h) and 66% and during the night (18:00-07:00 h). This differe...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Saran R,Sharma MC,Gupta AK,Sinha SP,Kar SK

    更新日期:1997-12-01 00:00:00

  • Molecular context of Schistosoma mansoni transmission in the molluscan environments: A mini-review.

    abstract::Schistosoma mansoni, being transmitted by some freshwater Biomphalaria snails, is a major causative agent of human schistosomiasis. In the absence of effective vaccine and alternative drug designs to fight against the disease, and with the limitations of molluscicide application, developing more efficient strategies t...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审


    authors: Famakinde DO

    更新日期:2017-12-01 00:00:00

  • Bioinformatics analysis of four proteins of Leishmania donovani to guide epitopes vaccine design and drug targets selection.

    abstract::Visceral leishmaniasis (VL) is a serious and widespread parasitic disease caused by Leishmania donovani complex. The threat of this fatal disease continues due to the lack of ideal drugs or vaccines. In this study, we selected Amastin, CaNA2, Kmp-11 and PDI proteins of Leishmania donovani for study, which are VL vacci...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: He J,Huang F,Li J,Chen Q,Chen D,Chen J

    更新日期:2019-03-01 00:00:00

  • In vitro antileishmanial activity of novel azoles (3-imidazolylflavanones) against promastigote and amastigote stages of Leishmania major.

    abstract::Leishmaniasis is a protozoan infectious disease widely distributed all around the world. First line drugs including antimoniales are insufficient due to resistance in endemic areas and high toxicity. Azole antifungals like ketoconazole (KCZ) are also used as antileishmanial agents for several decades. In the present s...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Shokri A,Emami S,Fakhar M,Teshnizi SH,Keighobadi M

    更新日期:2017-03-01 00:00:00

  • Seroprevalence and risk factors of glanders in working equines - Findings of a cross-sectional study in Punjab province of Pakistan.

    abstract::Glanders is an infectious and contagious bacterial disease of equines. A little is known about its seroprevalence and risk factors in working equines in countries where the disease is endemic. Also, there are no reports on prevalence of the disease in areas where there is a prior evidence of Burkholderia (B.) mallei d...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Ghori MT,Khan MS,Khan JA,Rabbani M,Shabbir MZ,Chaudhry HR,Ali MA,Muhammad J,Elschner MC,Jayarao BM

    更新日期:2017-12-01 00:00:00

  • Sequence analysis in partial genes of five isolates of Angiostrongylus cantonensis from Taiwan and biological comparison in infectivity and pathogenicity between two strains.

    abstract::Angiostrongylus cantonensis is the most common infectious agent causing eosinophilic meningitis and is present in Taiwan, Thailand and the Pacific islands. Clinical symptoms vary within different endemic regions, and their severity is probably dependent on the number of ingested parasites and the diversity among strai...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Lee JD,Chung LY,Wang LC,Lin RJ,Wang JJ,Tu HP,Wu ZD,Yen CM

    更新日期:2014-05-01 00:00:00

  • Factors associated with Schistosoma mansoni infection 5 years after selective treatment in a low endemic area in Brazil.

    abstract::Five years after a single dose treatment, prevalence, intensity and morbidity of schistosomiasis mansoni were evaluated in Agua Branca, a low endemic community in the South East Brazil (cure rate 94%). At community level, prevalence showed a decrease from 38.9 to 24.5% and the intensity of infection dropped from 119.5...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Disch J,Katz N,Pereira e Silva Y,de Gouvêa Viana L,Andrade MO,Rabello A

    更新日期:2002-02-01 00:00:00

  • Mapping the environmental suitability of etiological agent and tick vectors of Crimean-Congo hemorrhagic fever.

    abstract::Crimean-Congo hemorrhagic fever (CCHF) is one of the most important public health threats in many regions across Africa, Europe, and Asia. This study used ecological niche modeling analyses to map the environmental suitability of both CCHF virus (CCHFV), and its tick vectors (Amblyomma variegatum, Dermacentor marginat...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Okely M,Anan R,Gad-Allah S,Samy AM

    更新日期:2020-03-01 00:00:00

  • Trypanosoma cruzi strains, Tulahuen 2 and Y, besides the difference in resistance to oxidative stress, display differential glucose-6-phosphate and 6-phosphogluconate dehydrogenases activities.

    abstract::The drugs currently available for Chagas'disease treatment are unsatisfactory due to limited efficacy and toxic side effects, making the search for more specific pharmacological agents a priority. The components of the Trypanosoma cruzi trypanothione-dependent antioxidant system have been pointed out as potential chem...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Mielniczki-Pereira AA,Chiavegatto CM,López JA,Colli W,Alves MJ,Gadelha FR

    更新日期:2007-01-01 00:00:00

  • Detection and quantification of schistosome DNA in freshwater snails using either fluorescent probes in real-time PCR or oligochromatographic dipstick assays targeting the ribosomal intergenic spacer.

    abstract::Several DNA probes were designed for use in real-time polymerase chain reaction (PCR) assays to target sequence variation within the ribosomal intergenic spacer (IGS) of schistosomes. A sub-section of the IGS (∼300bp) was amplified, with cross-specific primers, after which group-specific fluorescent, locked nucleic ac...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Kane RA,Stothard JR,Rollinson D,Leclipteux T,Evraerts J,Standley CJ,Allan F,Betson M,Kaba R,Mertens P,Laurent T

    更新日期:2013-11-01 00:00:00

  • Potential sexual transmission of Trypanosoma cruzi in mice.

    abstract::Infection with the protozoan parasite Trypanosoma cruzi, the etiologic agent of human Chagas disease, results in life-long infection. Infective trypomastigotes circulate in the bloodstream and have the capacity to infect any cell type, including reproductive tissue. This study sought to assess the potential for sexual...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Martin DL,Lowe KR,McNeill T,Thiele EA,Roellig DM,Zajdowicz J,Hunter SA,Brubaker SA

    更新日期:2015-09-01 00:00:00

  • Evaluation of indirect screening techniques for the detection of Schistosoma haematobium infection in an urban area, Dar es Salaam, Tanzania.

    abstract::393 primary school children were screened for Schistosoma haematobium using four indirect techniques (a) history of haematuria, (b) visual appearance of urine and use of chemical reagent strips to detect presence of (c) blood and (d) protein in urine. Results showed that the use of chemical reagent strips for the dete...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Sarda RK,Minjas JN,Mahikwano LF

    更新日期:1985-09-01 00:00:00

  • Intestinal helminth coinfection is associated with mucosal lesions and poor response to therapy in American tegumentary leishmaniasis.

    abstract::The most severe clinical form of American tegumentary leishmaniasis (ATL) due to Leishmania braziliensis is mucosal leishmaniasis (ML), characterized by destructive lesions in the facial mucosa. We performed a retrospective cohort study of 109 ATL patients from Rio de Janeiro State, Brazil, where ATL is caused by L. b...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Azeredo-Coutinho RB,Pimentel MI,Zanini GM,Madeira MF,Cataldo JI,Schubach AO,Quintella LP,de Mello CX,Mendonça SC

    更新日期:2016-02-01 00:00:00

  • Identification of antigenically distinct populations of Leishmania (Viannia) guyanensis from Manaus, Brazil, using monoclonal antibodies.

    abstract::Eighty Leishmania isolates from patients who contracted cutaneous leishmaniasis in the Manaus region, Amazonas State, Brazil, were identified as Leishmania (Viannia) guyanensis by the electrophoretic profiles of six enzymes. None reacted with the species-specific monoclonal antibody B19. Two L. (V.) guyanensis subpopu...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Romero GA,Ishikawa E,Cupolillo E,Toaldo CB,Guerra MV,Paes MG,Macêdo Vde O,Shaw JJ

    更新日期:2002-04-01 00:00:00

  • Sero-epidemiological assessment and diagnosis of visceral leishmaniasis in an endemic locality using Fast Agglutination Screening Test (FAST).

    abstract::The Fast Agglutination Screening Test (FAST) was employed on sera obtained from an endemic area of visceral leishmaniasis in southwestern Ethiopia, in February 2000. The study involved (i) active case detection among 1575 residents of two villages; and (ii) passive case detection in an outpatient clinic. Sera of 1587 ...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Hailu A,Kroon CC,Schoone GJ,Berhe N,Schallig HD,Kager PA

    更新日期:2002-08-01 00:00:00

  • Entomological and epidemiological attributes for malaria transmission and implementation of vector control in southern Iran.

    abstract::Bashagard is an important malaria endemic area in south of Iran. An epidemiological and entomological survey was performed during 2002-2010. The aim of study was to determine malaria situation, species composition of anopheline mosquitoes and susceptibility status of main vectors to insecticides/larvicides. A total of...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Hanafi-Bojd AA,Vatandoost H,Oshaghi MA,Haghdoost AA,Shahi M,Sedaghat MM,Abedi F,Yeryan M,Pakari A

    更新日期:2012-02-01 00:00:00

  • Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods.

    abstract::Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi and a risk factor for parasite transmission. In this study we assessed the relative performance of a polymerase chain reaction assay targeted to minicircle DNA (kDNA-PCR) in reference to conventional serological tests, a rapid dipstick test...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Enriquez GF,Cardinal MV,Orozco MM,Schijman AG,Gürtler RE

    更新日期:2013-06-01 00:00:00

  • Effect of niloticin, a protolimonoid isolated from Limonia acidissima L. (Rutaceae) on the immature stages of dengue vector Aedes aegypti L. (Diptera: Culicidae).

    abstract::The aim of the present study was to evaluate the mosquitocidal activity of fractions and a compound niloticin from the hexane extract of Limonia acidissima L. leaves on eggs, larvae and pupae of Aedes aegypti L. (Diptera: Culicidae). In these bioassays, the eggs, larvae and pupae were exposed to concentrations of 2.5,...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Reegan AD,Gandhi MR,Paulraj MG,Balakrishna K,Ignacimuthu S

    更新日期:2014-11-01 00:00:00

  • Relationship between leaf litter identity, expression of cytochrome P450 genes and life history traits of Aedes aegypti and Aedes albopictus.

    abstract::The role of toxic component of leaf litter in mediating the outcome of mosquito species interactions is not well documented. To examine the effect of leaf litter toxins on mosquito performance and interspecific interactions, we reared monospecific and heterospecific cultures of Aedes aegypti L. and Aedes albopictus Sk...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Kim CH,Muturi EJ

    更新日期:2012-04-01 00:00:00

  • Studies on the effect of the anti-phagocytic agent cytochalasin B on Leishmania-macrophage interaction.

    abstract::Mouse peritoneal exudate cells were cultured on coverslips in Eagle's Basal Essential Medium. The adhering cells were infected with promastigotes of three different species of Leishmania. After 8 h incubation, the macrophages were fixed and stained, and a total of one hundred cells were counted. The rates of infection...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Ardehali SM,Khoubyar K,Rezai HR

    更新日期:1979-03-01 00:00:00

  • B lymphocyte population and immunoglobulins in Indian kala-azar in response to chemotherapy.

    abstract::The levels of immunoglobulin classes (IgG, IgM and IgA) along with B lymphocyte population size were estimated in 24 kala-azar (KA) patients and results were compared with those obtained from 30 controls. A marked increase in the level of IgG and to a lesser extent in that of IgM was noted in the sera of KA patients. ...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Neogy AB,Nandy A,Dastidar BG,Chowdhury AB

    更新日期:1986-09-01 00:00:00

  • Schistosoma rodhaini: intramolluscan larval development, migration and replication processes of daughter sporocysts.

    abstract::The larval development of Schistosoma rodhaini in Biomphalaria glabrata presents two features of interest. The first concerns the migration of daughter sporocysts: only a fraction of the daughter sporocysts produced by the mother sporocyst migrate directly towards the digestive gland of the snail and become cercarioge...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Théron A,Touassem R

    更新日期:1989-01-01 00:00:00

  • Trypanosoma cruzi: comparative studies of infectivity of parasites ingested by Triatoma infestans and those present in their feces.

    abstract::Artificial feeding of the insects with whole blood containing trypomastigotes resulted in triatome infection; the parasites were present in the host feces after 15--30 days and, when inoculated into mice, elicited parasitemia in 100% of the cases and deaths in 20--70%. This mortality indicates a reduction of the origi...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Lammel EL,de Isola EL,Korn C,Gonzalez Cappa SM

    更新日期:1981-06-01 00:00:00

  • An outbreak of peste des petits ruminants (PPR) in camels in the Sudan.

    abstract::In mid-August 2004, an outbreak of a previously unknown fatal disease of camels was reported to Kassala State veterinary authorities. Several areas in the state were visited during August-October 2004 to collect epidemiological data and specimens for diagnosis. Clinically the disease was characterized by sudden death ...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Khalafalla AI,Saeed IK,Ali YH,Abdurrahman MB,Kwiatek O,Libeau G,Obeida AA,Abbas Z

    更新日期:2010-11-01 00:00:00

  • Morphological and genetic characterization of Echinococcus granulosus in the Slovak Republic.

    abstract::The present study was undertaken to characterize more thoroughly aetiological agent of cystic echinococcosis that still represents a health problem in the Slovak Republic. Sequencing of the mitochondrial ND1 gene, Random Amplified Polymorphic DNA (RAPD) analysis and isoenzyme analyses were used to characterize genetic...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Turceková L,Snábel V,D'Amelio S,Busi M,Dubinský P

    更新日期:2003-02-01 00:00:00

  • Larval stress alters dengue virus susceptibility in Aedes aegypti (L.) adult females.

    abstract::In addition to genetic history, environmental conditions during larval stages are critical to the development, success and phenotypic fate of the Aedes aegypti mosquito. In particular, previous studies have shown a strong genotype-by-environment component to adult mosquito body size in response to optimal vs stressed ...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Kang DS,Alcalay Y,Lovin DD,Cunningham JM,Eng MW,Chadee DD,Severson DW

    更新日期:2017-10-01 00:00:00

  • Plasticity of the histone H2A genes in a Brazilian and six Colombian strains of Trypanosoma cruzi.

    abstract::The analysis of three recombinant clones containing the histone H2A locus isolated from a genomic library of Trypanosoma cruzi DNA shows that the H2A gene loci are formed by 1.2 and 0.76 kb long intercalated units organized in a head-to-tail tandem array. The difference in length between the two gene units is due to t...

    journal_title:Acta tropica

    pub_type: 杂志文章


    authors: Thomas MC,Olivares M,Escalante M,Marañón C,Montilla M,Nicholls S,López MC,Puerta C

    更新日期:2000-03-25 00:00:00