Abstract：
:Brucellosis is caused by the genus Brucella. Brucella is widely distributed in cattle, swine, sheep, goat and other mammals including human. Animal brucellosis causes severe economic losses and affects related international transportation and trade. Human brucellosis causes both acute and chronic symptoms of multi-organ dysfunction. Brucella type IV secretion system (T4SS) VirB5 was required for macrophages infection and essential for virulence in mice. VirB5 is located on the cell surface and serves as a specific adhesin targeting host cell receptors. The aim of this study was to isolate and characterize a specific human domain antibody against Brucella abortus (B. abortus) VirB5 from human single domain antibody (sdAb or VHH) phage display library. Following five rounds of screening, an sdAb named as BaV5VH4 showed the highest affinity by enzyme-linked immunosorbent assay (ELISA). Its interaction with B. abortus VirB5 was verified by binding assay, dot blot and molecular docking. These findings in this paper could greatly help elucidate the molecular mechanisms of Brucella infection, and accelerate the development of sdAbs-based vaccines and neutralizing therapeutics of brucellosis.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Deng H,Zhou J,Gong B,Xiao M,Zhang M,Pang Q,Zhang X,Zhao B,Zhou Xdoi
10.1016/j.actatropica.2019.05.017subject
Has Abstractpub_date
2019-09-01 00:00:00pages
105026eissn
0001-706Xissn
1873-6254pii
S0001-706X(18)30923-9journal_volume
197pub_type
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