Abstract：
:Sodium Antimony Gluconate (SAG) is currently used worldwide as the first-line drugs for the treatment of visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) since 1940s. Unfortunately, the resistance of Leishmania parasite to this drug is increasing in several parts of the world. The mechanism of drug resistance in clinical isolates is still not very clear. Earlier, we have established a differentiation between six clinical isolates as sensitive and resistant on the basis of their sensitivity to SAG in vitro and in vivo as well as expression of proteophosphoglycan contents. In this preliminary study, we have further analyzed these isolates on the basis of their genetic diversity, molecular variance and phylogenetic structure using for the first time, a fingerprinting approach--amplified fragment length polymorphism (AFLP). Altogether 2338 informative AFLP bands were generated using 10 selective primer combinations. Percentage of polymorphism was 55.35%. A number of unique AFLP markers (217) were also identified in these strains. It was deduced that a higher rate of variations occurred among Leishmania clinical isolates which indicate the shifting of drug sensitive nature of parasite towards resistant condition.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Kumar A,Boggula VR,Sundar S,Shasany AK,Dube Adoi
10.1016/j.actatropica.2009.01.005subject
Has Abstractpub_date
2009-04-01 00:00:00pages
80-5issue
1eissn
0001-706Xissn
1873-6254pii
S0001-706X(09)00007-2journal_volume
110pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract：:This study identified the natural infection rate of Leishmania (Leishmania) infantum in Lutzomyia longipalpis sandflies collected in a neighborhood around a kennel, in Dracena, northwestern of São Paulo state. This region is highly endemic for visceral leishmaniasis in Brazil. Insects were captured during 2-3 nights m...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.01.012
更新日期：2018-05-01 00:00:00
abstract：:Essential oils (EOs) are potential tools for controlling Musca domestica L. In a fumigant assay, M. domestica adults treated with Citrus sinensis EO (LC50=3.9mg/dm(3)), with (4R)(+)-limonene (95.1%) being its main component, died within 15min or less. The terpenes absorbed by the flies and their metabolites, analyzed ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.03.009
更新日期：2013-07-01 00:00:00
abstract：:Chau Cuica was the name given by the regional government of Loreto in Peru for its school-based deworming program which was initiated in 2012 with a donation of mebendazole from an international non-governmental organization. Embedded in the program from the start was a sentinel surveillance component which consisted ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2016.04.001
更新日期：2016-07-01 00:00:00
abstract：:A vaccine to protect sheep, goats, and bovines against hydatid disease caused by the cysts of Echinococcus granulosus is prepared as a recombinant fusion protein expressed in Escherichia coli. Solubilised inclusion bodies are injected, together with Quil A, subcutaneously on two occasions 1 month or more apart, and in...
journal_title：Acta tropica
pub_type： 指南,杂志文章,实务指引,评审
doi：10.1016/s0001-706x(02)00219-x
更新日期：2003-02-01 00:00:00
abstract：:Definitions of the term 'challenge' as applied to the African trypanosomiases are reviewed. Data from one West and one East African site show simple linear relationships between the incidence of trypanosomiasis in both humans and animals, and either the amount of man-tsetse contact, or the Apparent Density of flies. D...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：
更新日期：1985-03-01 00:00:00
abstract：BACKGROUND:Mosquitoes are responsible for spreading many diseases and their populations are susceptible to environmental changes. The ecosystems in the Three Gorges Region were probably altered because of changes to the environment during the construction of the Three Gorges Dam (TGD), the world's largest hydroelectric...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.03.008
更新日期：2018-06-01 00:00:00
abstract：:The influence of live Brugia malayi parasites and a Sephadex G-200 fraction of the adult parasite extract (BmAFII) on the progression of Leishmania donovani infection was studied. Inbred hamsters were first infected with B. malayi infective 3rd stage larvae (L3), adult worms or microfilariae (mf), and then with L. don...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2008.01.007
更新日期：2008-05-01 00:00:00
abstract：:The spatial and temporal dynamics of mosquitoes in stream pools were examined in a biosphere reserve of the Southern Western Ghats, India. The immature mosquitoes in stream pools were collected from stream substrates of bedrock pool, boulder cavity and sand puddle. The collected larvae and pupae were reared and identi...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2015.09.024
更新日期：2015-12-01 00:00:00
abstract：:Cystic echinococcosis (CE) is a worldwide zoonosis caused by the Echinococcus granulosus larval stage. The currently available therapy for this disease is based on benzimidazoles, which are rarely curative and cause several adverse effects. Therefore, new treatment options are needed. Octreotide (Oct) is a somatostati...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2019.105312
更新日期：2020-03-01 00:00:00
abstract：:Leishmania (Mundinia) orientalis is a newly described species causing human leishmaniasis in Thailand whose natural vector is unknown. L. orientalis infections in sand flies and/or biting midges under laboratory conditions have not been previously investigated. In this study, the development of L. orientalis in two ex...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2019.105157
更新日期：2019-11-01 00:00:00
abstract：:Symptomatic chronic Chagas disease affects up to 40% of patients infected with Trypanosoma cruzi. The lack of reliable early markers of cure after therapy hinders disease management and clinical trials with new drugs. We performed a study with 18 months of follow-up to compare changes in immune parameters and T. cruzi...
journal_title：Acta tropica
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.actatropica.2016.09.010
更新日期：2016-12-01 00:00:00
abstract：:The Kato-Katz technique is the method routinely used for diagnosing human schistosomiasis mansoni by estimating faecal egg burdens. To improve the sensitivity of faecal diagnosis, we established and validated a novel separation technique based upon the greater density of viable schistosome eggs relative to faecal mate...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(02)00089-x
更新日期：2002-08-01 00:00:00
abstract：:The Clean India Mission is a national campaign that aims for complete elimination of open defecation from the country. In India, 564 million people do not have access to toilets and defecate in the open environment. The 'Millennium development goals' have given increased weightage to elimination of open defecation for...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2018.06.024
更新日期：2018-10-01 00:00:00
abstract：:The role of toxic component of leaf litter in mediating the outcome of mosquito species interactions is not well documented. To examine the effect of leaf litter toxins on mosquito performance and interspecific interactions, we reared monospecific and heterospecific cultures of Aedes aegypti L. and Aedes albopictus Sk...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.12.006
更新日期：2012-04-01 00:00:00
abstract：:Disease control programmes are an intrinsic part of health systems. Neglected tropical disease (NTD) control is a clear case in point. While there is a growing consensus that NTD control and health services are linked, with important mutual impacts, little is known of what actually happens at the interface between the...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2011.02.017
更新日期：2011-09-01 00:00:00
abstract：:Greece has been recently affected by several mosquito borne diseases with the West Nile Virus (WNV) outbreak in 2010 being one of the largest reported in Europe. Currently at the epicenter of an economic and refugee crisis and visited by over 16 million tourists a year the integrated management of diseases transmitted...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.06.005
更新日期：2017-10-01 00:00:00
abstract：:The many mathematical models of the transmission dynamics of schistosomes that have been published since 1965 have had little impact on field studies or on the design of schistosome control programmes. At least in part, this is due to limited interaction between theoretician and field worker, resulting in unrealistic ...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/0001-706x(91)90077-w
更新日期：1991-09-01 00:00:00
abstract：:We examined the malaria situation among 489 children under 5 years of age in the rural villages of Aboh Mbaise, Nigeria, using a combination of a standard questionnaire technique and laboratory diagnosis to confirm clinical observations. The results show a high prevalence rate of 52.8% for Plasmodium falciparum in thi...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/0001-706x(90)90061-4
更新日期：1990-10-01 00:00:00
abstract：:With the paradigm shift from the reduction of morbidity and mortality to the interruption of transmission, the focus of malaria control broadens from symptomatic infections in children ≤5 years of age to include asymptomatic infections in older children and adults. In addition, as control efforts intensify and the num...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.11.009
更新日期：2012-03-01 00:00:00
abstract：:The 4-year (2006-2010) alliance to optimize schistosomiasis control and transmission surveillance in sub-Saharan Africa (CONTRAST), funded by the European Commission, stands out as a 'game-changer' by successfully harnessing and combining new as well as previously available capabilities for schistosomiasis control in ...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2013.08.016
更新日期：2013-11-01 00:00:00
abstract：:In history disease has caused social and economic damage. Dengue is an illness typically found in the tropics that has affected more and more people. In Brazil, according to the Brazilian Institute of Geography and Statistics (IBGE), in 2013 at least 12.9% of the population (25.8 million) reported already having had d...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.11.016
更新日期：2018-02-01 00:00:00
abstract：:In this study, 27 CDC traps were modified with various attractive features and compared with a CDC trap with no light source or baits to evaluate the effects on attraction to Phlebotomus papatasi (Scopoli) north of the Dead Sea near Jericho. Attractive features included CO2, lights, colored trap bodies, heat, moisture...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2014.06.007
更新日期：2015-01-01 00:00:00
abstract：:Loop-mediated isothermal amplification (LAMP) is a novel molecular method that accelerates and facilitates DNA amplification and detection under isothermal conditions. It represents a revolution in molecular biology by reducing the high cost, turnaround time and technicality of polymerase chain reaction and other ampl...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2012.02.004
更新日期：2012-06-01 00:00:00
abstract：:Although, pleural (PT) and disseminated tuberculosis (DT) have been considered as extreme endpoints of the Th1-Th2 immunological spectrum of the Mycobacterium tuberculosis infection, these conditions can occur together. The presence of PT and DT could be explained by (1) PT as primary condition, with progression of HI...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2007.07.009
更新日期：2007-11-01 00:00:00
abstract：:A cross-sectional study was conducted to investigate the seroprevalence of brucellosis and identify risk factors in exotic and cross bred cattle in Ethiopia. A total of 2334 cattle from 273 farms were tested serially for Brucella antibodies using the Rose Bengal Plate Test (RBPT) and the Compliment Fixation Test (CFT)...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.02.015
更新日期：2013-06-01 00:00:00
abstract：:In Brazil, there are near 20 genera and almost 120 species of scorpions of which 95% reproduce sexually. Parthenogenetic reproduction, however, may also take place. To gain insight into useful molecular markers in parthenogenetic scorpion species, we studied DNA polymorphism using two molecular approaches: simple sequ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2009.07.002
更新日期：2009-11-01 00:00:00
abstract：:We focused on the epidemiology of alveolar echinococcosis especially in Japan and discussed control measures to prevent an epidemic. No effective control measures against alveolar echinococcosis have been identified thus far because it is difficult to fully understand the ecology of the parasite and its hosts, i.e. th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2003.09.001
更新日期：2003-12-01 00:00:00
abstract：:Artificial feeding of the insects with whole blood containing trypomastigotes resulted in triatome infection; the parasites were present in the host feces after 15--30 days and, when inoculated into mice, elicited parasitemia in 100% of the cases and deaths in 20--70%. This mortality indicates a reduction of the origi...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：:This paper reviews various imaging techniques in different organs. Ultrasound is the most popular and readily available technique. Special emphasis is given to computerized tomography (CT) and magnetic resonance imaging (MRI) findings and their advantages for a confident diagnosis and treatment. ...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/s0001-706x(97)00052-1
更新日期：1997-09-15 00:00:00
abstract：:Brucellosis is endemic in the Republic of Kazakhstan, particularly in agro-pastoral areas. The purpose of this research is to study the epidemiological situation of brucellosis in livestock recorded in the Republic of Kazakhstan, and to identify the reasons why anti-brucellosis measures were not effective. The researc...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.12.028
更新日期：2019-03-01 00:00:00
