Abstract:
:The rapid, accurate diagnosis of Plasmodium spp. is essential for the effective control of malaria, especially in asymptomatic infections. In this study, we developed a sensitive, genus-specific, real-time quantitative PCR assay. It was compared with the microscopic examination of Giemsa-stained blood smears and two different molecular diagnostic techniques: nested PCR and multiplex PCR. For the effective quantitative detection of malaria parasites, all reagents were designed with a lyophilized format in one tube. Plasmodium was detected successfully in all 112 clinically suspected malaria patients, including 32 individuals with low parasitemia (1-100 parasites/μl). The sensitivity threshold was 0.2 parasites/μl and no PCR-positive reaction occurred when malaria parasites were not present. This may be a useful method for detecting malaria parasites in endemic areas.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Hwang SY,Kim SH,Lee GY,Hang VT,Moon CS,Shin JH,Koo WL,Kim SY,Park HJ,Park HO,Kho WGdoi
10.1016/j.actatropica.2011.05.006subject
Has Abstractpub_date
2011-10-01 00:00:00pages
40-5issue
1-2eissn
0001-706Xissn
1873-6254pii
S0001-706X(11)00165-3journal_volume
120pub_type
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