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A novel real-time PCR assay for the detection of Plasmodium falciparum and Plasmodium vivax malaria in low parasitized individuals.

Abstract：

:The rapid, accurate diagnosis of Plasmodium spp. is essential for the effective control of malaria, especially in asymptomatic infections. In this study, we developed a sensitive, genus-specific, real-time quantitative PCR assay. It was compared with the microscopic examination of Giemsa-stained blood smears and two different molecular diagnostic techniques: nested PCR and multiplex PCR. For the effective quantitative detection of malaria parasites, all reagents were designed with a lyophilized format in one tube. Plasmodium was detected successfully in all 112 clinically suspected malaria patients, including 32 individuals with low parasitemia (1-100 parasites/μl). The sensitivity threshold was 0.2 parasites/μl and no PCR-positive reaction occurred when malaria parasites were not present. This may be a useful method for detecting malaria parasites in endemic areas.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Hwang SY,Kim SH,Lee GY,Hang VT,Moon CS,Shin JH,Koo WL,Kim SY,Park HJ,Park HO,Kho WG

doi

10.1016/j.actatropica.2011.05.006

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

40-5

issue

1-2

eissn

0001-706X

issn

1873-6254

pii

S0001-706X(11)00165-3

journal_volume

120

pub_type

杂志文章

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