当前位置： SCI文献检索 > ACTA TROPICA期刊下所有文献 > Blood meal identification and parasite detection in laboratory-fed and field-captured Lutzomyia longipalpis by PCR using FTA databasing paper.

Blood meal identification and parasite detection in laboratory-fed and field-captured Lutzomyia longipalpis by PCR using FTA databasing paper.

Abstract：

:The phlebotomine sand fly Lutzomyia longipalpis takes blood from a variety of wild and domestic animals and transmits Leishmania (Leishmania) infantum chagasi, etiological agent of American visceral leishmaniasis. Blood meal identification in sand flies has depended largely on serological methods but a new protocol described here uses filter-based technology to stabilise and store blood meal DNA, allowing subsequent PCR identification of blood meal sources, as well as parasite detection, in blood-fed sand flies. This technique revealed that 53.6% of field-collected sand flies captured in the back yards of houses in Teresina (Brazil) had fed on chickens. The potential applications of this technique in epidemiological studies and strategic planning for leishmaniasis control programmes are discussed.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Sant'Anna MR,Jones NG,Hindley JA,Mendes-Sousa AF,Dillon RJ,Cavalcante RR,Alexander B,Bates PA

doi

10.1016/j.actatropica.2008.06.003

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

230-7

issue

3

eissn

0001-706X

issn

1873-6254

pii

S0001-706X(08)00177-0

journal_volume

107

pub_type

杂志文章

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