Abstract：
:Resistance of trypanosomes to melarsoprol is ascribed to reduced uptake of the drug via the P2 nucleoside transporter. The aim of this study was to look for evidence of drug resistance in Trypanosoma brucei gambiense isolates from sleeping sickness patients in Ibba, South Sudan, an area of high melarsoprol failure rate. Eighteen T. b. gambiense stocks were phenotypically and only 10 strains genotypically characterized. In vitro, all isolates were sensitive to melarsoprol, melarsen oxide, and diminazene. Infected mice were cured with a 4 day treatment of 2.5mg/kg bwt melarsoprol, confirming that the isolates were sensitive. The gene that codes for the P2 transporter, TbATI, was amplified by PCR and sequenced. The sequences were almost identical to the TbAT1(sensitive) reference, except for one point mutation, C1384T resulting in the amino acid change proline-462 to serine. None of the described TbAT1(resistant)-type mutations were detected. In a T. b. gambiense sleeping sickness focus where melarsoprol had to be abandoned due to the high incidence of treatment failures, no evidence for drug resistant trypanosomes or for TbAT1(resistant)-type alleles of the P2 transporter could be found. These findings indicate that factors other than drug resistance contribute to melarsoprol treatment failures.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Maina N,Maina KJ,Mäser P,Brun Rdoi
10.1016/j.actatropica.2007.07.007subject
Has Abstractpub_date
2007-11-01 00:00:00pages
84-90issue
2-3eissn
0001-706Xissn
1873-6254pii
S0001-706X(07)00186-6journal_volume
104pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract：:Personal protection measures have become an important tool against mosquito nuisance. The severity of mosquito nuisance and the type and costs of personal protection measures in the Pondicherry region in South India have been investigated, using a structured questionnaire. The number of respondents sampled was 300 in ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(03)00155-4
更新日期：2003-09-01 00:00:00
abstract：:During a period of 2 years, the ecology of Bulinus globosus was studied in 8 habitats in two streams near Ifakara, SE-Tanzania. The relative Bulinus densities were followed monthly. Two different methods for estimating snail densities (man/time vs. palmleaf traps) gave comparable results. Bulinus densities were consta...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Peripheral blood smears of 20 patients with Indian Kala-azar (KA) were collected throughout the day and night and examined for presence of Leishmania amastigotes. Amastigotes were detected in 46% of peripheral blood samples taken during the day (08:00-17:00 h) and 66% and during the night (18:00-07:00 h). This differe...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(97)00104-6
更新日期：1997-12-01 00:00:00
abstract：:Antibodies to the Pfs2400 gametocyte antigen have been shown to inhibit the development of Plasmodium falciparum in anophelines and therefore this antigen is a candidate for a transmission-blocking vaccine. To test seasonal variation of these antibodies under field conditions, sera from 72 individuals were collected t...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(96)00620-1
更新日期：1997-02-01 00:00:00
abstract：:We focused on the epidemiology of alveolar echinococcosis especially in Japan and discussed control measures to prevent an epidemic. No effective control measures against alveolar echinococcosis have been identified thus far because it is difficult to fully understand the ecology of the parasite and its hosts, i.e. th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2003.09.001
更新日期：2003-12-01 00:00:00
abstract：:This review paper examines the evidence from published and previously unpublished sources for the efficacy and safety of albendazole (ABZ) obtained in the last 12 years. Benzimidazole treatment alone is not ideal, requiring prolonged administration over many weeks, with an unpredictable outcome in terms of response ra...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/s0001-706x(96)00640-7
更新日期：1997-04-01 00:00:00
abstract：:Many societies use locally sourced mosquito nets made from a variety of materials. For protecting against malaria these require regular re-treatment with insecticide. K-O Tab 1-2-3 is a 'dip-it-yourself' long-lasting formulation with time-limited interim recommendation from WHO for treatment of washed white and colour...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2009.06.005
更新日期：2009-10-01 00:00:00
abstract：:Giardiasis is a major problem in León, Nicaragua, yet despite this no data are available regarding the prevalence of different Giardia genotypes in this area. To address this question, a molecular analysis of Giardia isolates from humans and dogs living in the same area in León, Nicaragua was performed. Giardia isolat...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2008.01.004
更新日期：2008-04-01 00:00:00
abstract：:Palms are ubiquitous across Neotropical landscapes, from pristine forests or savannahs to large cities. Although palms provide useful ecosystem services, they also offer suitable habitat for triatomines and for Trypanosoma cruzi mammalian hosts. Wild triatomines often invade houses by flying from nearby palms, potenti...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2015.07.005
更新日期：2015-11-01 00:00:00
abstract：:This study aimed to investigate the prevalence of cercarial infections in freshwater snails collected from the Chai Nat province of Central Thailand. Moreover, we aimed to develop a duplex PCR technique, using the ITS2 and candidate MT-ND1 gene, to determine the dissemination of H. taichui and H. pumilio, respectively...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105795
更新日期：2020-12-10 00:00:00
abstract：:Leptospirosis is a zoonotic disease that poses public health and ecological threats worldwide. In New Zealand (NZ), incidence of the disease is relatively high compared to other developed countries. The aim of this review was to describe the epidemiological status, ecological risk of leptospirosis and prevention in NZ...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2018.08.021
更新日期：2018-12-01 00:00:00
abstract：:The increased insecticides resistance by vectors and the ecological harm imposed by insecticides to beneficial organisms drawback mosquitoes chemical control efforts. Biological control would reduce insecticides tolerance and yet biodiversity friend. The predatory and non-predatory effects of Gambusia affinis and Cara...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2014.11.012
更新日期：2015-02-01 00:00:00
abstract：:Symptomatic chronic Chagas disease affects up to 40% of patients infected with Trypanosoma cruzi. The lack of reliable early markers of cure after therapy hinders disease management and clinical trials with new drugs. We performed a study with 18 months of follow-up to compare changes in immune parameters and T. cruzi...
journal_title：Acta tropica
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.actatropica.2016.09.010
更新日期：2016-12-01 00:00:00
abstract：:The effect of vaccines against the pre-erythrocytic, the erythrocytic and the sexual stages of malaria on the transmission of malaria was examined using a simulation model of seasonal or epidemic transmission. The primary outcome of the model was the number of cases of malaria present at the end of the transmission se...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/0001-706x(93)90013-2
更新日期：1993-01-01 00:00:00
abstract：:India and other Southeast Asian countries are severely affected by Japanese encephalitis (JE), one of the deadliest vector-borne disease threat to human health. Several epidemiological observations suggest climate variables play a role in providing a favorable environment for mosquito development and virus transmissio...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105701
更新日期：2020-12-01 00:00:00
abstract：:Cell lysates from 16 strains of eight Leishmania species were used to test haemagglutination activity (HA) against a variety of RBC. HA was detected using native or neuraminidase-treated rabbit RBC; it was found in promastigotes of all the Leishmania strains tested and in axenic amastigotes of L. mexicana. The HA was ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(97)00069-7
更新日期：1997-10-14 00:00:00
abstract：:A survey was undertaken between November 1994 and January 1995 to assess the knowledge and practice with respect to malaria of 1946 randomly selected households in endemic urban and rural areas of Hormozgan, Kerman and Sistan and Baluchistan Provinces, southeast Iran. Knowledge of inhabitants was relatively poor in th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(96)00609-2
更新日期：1997-04-15 00:00:00
abstract：:The vector species Aedes albopictus (Skuse, 1894) was recorded in Turkey for the first time, near the Greek border, in 2011 and a high risk of expansion towards Aegean and Mediterranean coasts of Turkey was estimated. A preliminary study was planned to evaluate the possibility of creating a satellite mass rearing faci...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2016.03.032
更新日期：2016-07-01 00:00:00
abstract：:The Japanese Encephalitis (JE) virus circulation in Indonesia was first documented in Lombok in 1960, and the virus was first isolated in 1972 from Culex tritaeniorhynchus in Bekasi, West Java and Kapuk, West Jakarta. Since then, Indonesia has been recognized as an endemic country for JE transmission. Up to now, JE ca...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2018.08.017
更新日期：2018-11-01 00:00:00
abstract：:Domestic reinfestations by triatomine bugs were monitored after application of deltamethrin and apparent elimination of Triatoma infestans in Amama and other nearby rural villages, north-west Argentina, from 1992 to 1996. The five methods used were sensor boxes, sheets of pink typing-paper, timed manual catches by a s...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(98)00096-5
更新日期：1999-03-15 00:00:00
abstract：:The drawback of existing techniques for obtaining intact granulomas and granuloma cells have been, (1) lack of clean intact granulomas without adhering liver tissues, (2) isolating inadequate numbers of clean intact granulomas, and (3) obtaining low numbers of viable granuloma cells from the granulomas. This work aims...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(98)00034-5
更新日期：1998-06-30 00:00:00
abstract：:Cryptosporidium is one of the most widespread protozoan parasites that infects domestic and wild animals and is considered the second major cause of diarrhea and death in children after rotavirus. So far, around 20 distinct species are known to cause severe to moderate infections in humans, of which Cryptosporidium ho...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2017.10.023
更新日期：2018-08-01 00:00:00
abstract：:Thymidylate kinase (TMK) is a potential chemotherapeutic target because it is directly involved in the synthesis of deoxythymidine triphosphate, which is an essential component for DNA synthesis. The gene encoding thymidylate kinase of Brugia malayi was amplified by PCR and expressed in Escherichia coli. The native mo...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2014.02.003
更新日期：2014-05-01 00:00:00
abstract：:The last decade has seen an increase in investment and concerted efforts by the Malawi Ministry of Health and partners to control malaria disease. This report summarizes what is known about the burden of malaria and the strategies being implemented to control it in Malawi. Over the past 5 years, roll out of treatment ...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2011.06.017
更新日期：2012-03-01 00:00:00
abstract：:The study aimed to establish if there was any relationship between the blood group of the human host and schistosomiasis prevalence, intensity, incidence and related organ pathology. Urine and stool specimens were collected from the 735 school children attending a rural school in Zimbabwe to determine the Schistosoma ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(97)00671-2
更新日期：1997-05-30 00:00:00
abstract：:Diagnosis of neurocysticercosis (NCC) is complicated because of the variability in clinical presentations and course of the disease where viability of parasite is a major determinant. The present study describes evaluation of ELISAs using Taenia solium metacestode somatic and excretory-secretory (ES) antigens for dete...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2009.01.002
更新日期：2009-04-01 00:00:00
abstract：:Malaria kills more than a million people a year, causes malady in about three hundred million people and poses risk to approximately 40% of the world's population living in malarious countries. This disease is re-emerging mainly due to the development of drug-resistant parasites and insecticide-resistant mosquitoes. T...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2009.10.024
更新日期：2010-06-01 00:00:00
abstract：:In Argentina, American Cutaneous Leishmaniasis (ACL) extends up to 29°S in the phytogeographic regions of the Yungas (west), Chaco (center) and Paranaense (east). Since the Phlebotominae vectors of this disease in the western Chaco (dry Chaco) are unknown, in the present work, we studied the natural infection in Phleb...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2012.04.008
更新日期：2012-08-01 00:00:00
abstract：:Plasmodium vivax merozoite surface protein 1 (PvMSP-1) is a leading malaria vaccine candidate. This protein is processed to give rise to various sized fragments during merozoite maturation. Here, we describe the analysis of genetic diversity in the 42 kDa C-terminal part of this protein among 33 Indian P. vivax isolat...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2008.08.011
更新日期：2008-10-01 00:00:00
abstract：:Trypanosomes were isolated from the midguts of Glossina palpalis palpalis, G. pallicera pallicera and G. nigrofusca nigrofusca captured around the village of Guediboua, South West of Daloa in Côte d'Ivoire. Seventy of the 124 isolates, obtained from 688 flies, were examined for four different kinds of trypanosome usin...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/0001-706x(94)00087-h
更新日期：1995-05-01 00:00:00
