Abstract:
:Selective activation of the platelet TXA2 receptor is sufficient to mediate concurrent aggregation, deaggregation and shape change (SC) responses without activation of known Gi-coupled receptors (Platelets 2003; 14: 89). However, Gi-coupled receptor activation strongly influences the hemostasis response in vivo. This study investigated the modulatory effects of two signaling pathways related to Gi-coupled receptor activation, stimulation of phosphoinositide 3-kinases (PI3Ks) and inhibition of adenylyl cyclase (AC), on the aggregation, deaggregation and SC components of the platelet activation response. A novel turbidimetric approach was applied to separate these responses and to characterize their pharmacology and kinetics. The SC response was more sensitive to TXA2 receptor activation (lower EC50 value) but less sensitive to a TXA2 receptor antagonist (higher Kd value) than the net aggregation response. Epinephrine and sulprostone, agonists of the Gi-coupled alpha2A-adrenoceptor and EP3 receptor, respectively, amplified the SC, decelerated deaggregation and enhanced net aggregation responses. SQ22536 and 2',5'-dideoxyadenosine, inhibitors of AC activity, elicited smaller but qualitatively similar effects. The PI3K inhibitor wortmannin did not affect the SC response but accelerated deaggregation and inhibited net aggregation. These data are consistent with a differential modulation of the platelet SC response by each pathway associated with Gi-coupled receptor activation, while both pathways cooperatively enhance the net aggregation response by decelerating deaggregation. We propose that the TXA2 receptor mediated concurrent platelet aggregation and SC responses, that are differentially modulated by different signaling pathways, provide a model for studying the underlying cellular pharmacology of platelet physiology.
journal_name
Plateletsjournal_title
Plateletsauthors
Maayani S,Schwarz TE,Patel ND,Craddock-Royal BD,Tagliente TMdoi
10.1080/09537100310001612380subject
Has Abstractpub_date
2003-09-01 00:00:00pages
359-74issue
6eissn
0953-7104issn
1369-1635pii
B7MJ9F9CPDLVWUN0journal_volume
14pub_type
杂志文章相关文献
PLATELETS文献大全abstract::Lipopolysaccharide (LPS, endotoxin) is a well-known causative agent of septic shock and disseminated intravascular coagulation. The action of LPS is related to the activation of many types of cells, including blood platelets. We examined the effects of different LPSs from Proteus mirabilis (smooth form LPS S1959 and r...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/0953710021000024385
更新日期:2002-11-01 00:00:00
abstract::Adrenaline is a platelet activator having a resting plasma concentration of <1 nmol/l that increases to a few nmol/l during stress. However, most in vitro assays only detect effects of adrenaline in micromolar concentrations. This makes it difficult to estimate the relevance of in vitro data for the in vivo situation....
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.672780
更新日期:2013-01-01 00:00:00
abstract::Pre-analytical variables interact with standard coagulation parameters. How these variables affect the platelet function analysis is not completely known. How collection site and puncture method affect multiple electrode aggregometry (MEA) and platelet function analyzer (PFA-100®) was compared regarding contact activa...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.689038
更新日期:2013-01-01 00:00:00
abstract::In recent years, there has been exponential growth in the interest in microvesicles, which is reflected by the number of publications. Initially referred to as "platelet dust" by Peter Wolf in 1967, platelet microvesicles (PMV) are now recognized as important mediators of intercellular communication. There are example...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537104.2016.1257114
更新日期:2017-05-01 00:00:00
abstract::Catastrophic antiphospholipid syndrome (CAPS) involves sudden multiorgan dysfunction from thrombosis due to antibodies that cause platelet activation and endothelial dysfunction. Treatment variably combines anticoagulation, corticosteroid use, therapeutic plasma exchange (TPE), and high-dose intravenous immunoglobulin...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1802414
更新日期:2020-08-07 00:00:00
abstract::The influence of three anti-platelet drugs, cilostazol, aspirin, and tirofiban, was investigated on platelet-leukocyte interaction by flow cytometry. When platelets and leukocytes were pre-incubated with anti-platelet drugs and stimulated by thrombin or collagen, cilostazol was found to inhibit platelet adhesion to mo...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100410001715583
更新日期:2004-08-01 00:00:00
abstract::To study the possibility of using thrombin generation tests in platelet-rich and platelet-poor plasma for evaluation of dual antiplatelet therapy efficacy in patients with coronary artery disease (CAD), following percutaneous coronary intervention. Venous blood was analyzed from CAD patients aged 53-75 years who had u...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1294680
更新日期:2018-03-01 00:00:00
abstract::The ram gene was isolated from a rat megakaryocyte cDNA library and encodes a GTP-binding protein with a Mr of 25 000. In order to clarify the presence of the ram protein (ram p25) in human platelets, we tried to purify ram p25 from the sodium cholate extract of human platelet membranes by a combination of DEAE-Sephac...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109309013227
更新日期:1993-01-01 00:00:00
abstract::Hermansky-Pudlak syndrome (HPS) is a rare form of syndromic oculocutaneous albinism caused by disorders in lysosome-related organelles. Ten genes are associated with different forms of HPS. HPS type 9 (HPS-9) is caused by biallelic variants of BLOC1S6. To date, only three patients with HPS-9 have been reported. We des...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1742315
更新日期:2020-04-03 00:00:00
abstract::The typical data presentation by flow cytometry of platelet suspensions stimulated with calcium ionophore A-23187, thrombin, C5b-9, or other agonists shows a unimodal decrease (or 'left-shift') in forward angle light scatter. Many reports in the literature interpret these findings as indicative of the appearance of sm...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100050129279
更新日期:2000-08-01 00:00:00
abstract::Cell-cell and cell-extracellular matrix interactions are mediated by a number of membrane glycoproteins. On the basis of structural homologies, several families of cell adhesion molecules (integrins, selectins, immunoglobulins, cadherins, leucine-rich glycoproteins) have been established. Since 1991, a new family of C...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109609023570
更新日期:1996-01-01 00:00:00
abstract::Information regarding the profile of reticulated platelets (RP) in ischemic cerebrovascular disease (CVD) patients is limited. Data from two prospective, observational, case-control studies were combined to compare the %RP using whole blood flow cytometry in patients ≤ 4 weeks of TIA/stroke onset (baseline, N = 210), ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1850670
更新日期:2020-12-21 00:00:00
abstract::A 38-year-old female patient was diagnosed with anemia for 3 years. Medical examination showed slight splenomegaly (250 × 62 mm), thrombocytopenia (platelets 51 × 109/L), anemia (Hb levels 107 g/L), and β-glucocerebrosidase activity (GBA) in leukocytes was lower than normal. Microscopic findings of bone marrow smear d...
journal_title:Platelets
pub_type: 信件
doi:10.1080/09537104.2017.1306044
更新日期:2017-12-01 00:00:00
abstract::The potential roles of serum butyrylcholinesterase (BChE) activity and platelet indices in type 1 diabetes (T1D) remain uncertain. We aimed to investigate the correlation among the platelet indices, serum BChE activity, and diabetic ketoacidosis (DKA). Sixty-one T1D patients, 29 patients with DKA, and 30 age- and sex-...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.735720
更新日期:2013-01-01 00:00:00
abstract::In humans and other mammals, Tityus discrepans (Td) scorpion envenomation produces a variety of systemic effects including respiratory distress, a generalized inflammatory reaction, modulation of blood pressure, fibrin formation, and platelet activation. For many of these effects, the venom components and underlying m...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2010.544343
更新日期:2011-01-01 00:00:00
abstract::The spleen, in addition to its role in immunity, plays key roles in erythrocyte maintenance and platelet sequestration. Loss of the spleen via splenectomy occurs in approximately 6.4 to 7.1 per 100 000 people per year globally, commonly as a life-saving emergency procedure in trauma and a therapeutic procedure in hema...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1732322
更新日期:2021-01-02 00:00:00
abstract::SUMMARY. Blood platelets exposed to hypotonic medium are known to undergo a regulatory volume decrease (RVD), mediated by increased conductive permeability of K(+) and Cl(-). It is presently shown that RVD in platelets is controlled by a lipoxygenase product, apparently by a selective regulation of K(+) permeability. ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109109005512
更新日期:1991-01-01 00:00:00
abstract::Platelet activation is an important process in the pathogenesis of atherothrombosis. However, the serial changes of platelet activation in atherosclerotic ischemic stroke have not been determined. In this study, we measured serially platelet expression of CD63 and P-selectin and platelet aggregability to ADP and colla...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100310001644024
更新日期:2004-02-01 00:00:00
abstract::Familial bleeding problems are frequently difficult to diagnose because currently used clinical tests cannot identify intracellular molecular defects of platelets. Using platelet proteomics, a comprehensive analytical tool, we diagnosed a family with severe bleeding problems of unknown origin with Quebec Platelet Diso...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100802010547
更新日期:2008-08-01 00:00:00
abstract::Thrombosis and bleeding are devastating adverse events in patients supported with blood-contacting medical devices (BCMDs). In this study, we delineated that high non-physiological shear stress (NPSS) caused platelet dysfunction that may contribute to both thrombosis and bleeding. Human blood was subjected to NPSS wit...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1384542
更新日期:2019-01-01 00:00:00
abstract::Platelets play a key role in thrombotic vascular occlusion at the side of an atherosclerotic plaque. Malondialdehyde (MDA) derivatives are components of oxidized LDL. Recent studies suggest enhanced adhesion of platelets on oxidized LDL, but evidence of MDA-modified LDL contribution to enhanced platelet adhesion is mi...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100500261590
更新日期:2006-03-01 00:00:00
abstract::Platelets from patients with type 2 diabetes show abnormalities in intracellular Ca(2+) homeostasis that are involved in platelet hyperaggregability and the development of thrombotic complications. Different Ca(2+) transport mechanisms have been reported to be altered in platelets from patients with type 2 diabetes, i...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2010.528813
更新日期:2011-01-01 00:00:00
abstract::Fibrin glue is a promising alternative for low-output enterocutaneous fistula closure. Bacterial flora colonizing inside the fistula tract, however, may limit the glue application. Single-donor-derived, platelet-rich materials were hypothesized in this study to have antimicrobial activity against Gram-negative microor...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.761685
更新日期:2013-01-01 00:00:00
abstract::The present study had two goals: first, to give a detailed description of a reliable method for full kinetic analysis of serotonin transporter (5HTt) on the membrane of human platelets, and second, as a main issue, to report on physiological influences on kinetic characteristics of this transmembrane transport on a la...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2010.487582
更新日期:2010-01-01 00:00:00
abstract::The purpose of the study was to evaluate safety, effects on platelet aggregation and pharmacokinetics of F(ab')(2) fragments of anti-glycoprotein (GP) IIb-IIIa murine monoclonal antibody FRaMon (F(ab')(2) FRaMon) upon its intravenous administration in patients undergoing high-risk coronary angioplasty. Patients were t...
journal_title:Platelets
pub_type: 临床试验,杂志文章
doi:10.1080/0953710021000057839
更新日期:2002-12-01 00:00:00
abstract::Abstract Familial platelet disorder with predisposition to acute myeloid leukemia (FPD/AML) is an autosomal dominant disorder characterized by mild to moderate thrombocytopenia with or without its impaired function, inherited RUNX1 mutation and high incidence of myeloid malignancy, such as myelodysplastic syndrome or ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2013.818636
更新日期:2014-01-01 00:00:00
abstract::The SDF-1-CXCR4 axis plays an essential role in the regulation of platelet production, by directing megakaryocyte (MK) migration toward the vascular niche, thus allowing terminal maturation and proplatelet formation, and also regulates platelet function in an autocrine manner. Inherited thrombocytopenias (IT) comprise...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1254763
更新日期:2017-09-01 00:00:00
abstract::Thrombocytopenia is a clinically relevant outcome in HIV. However, the epidemiology of this condition, including frequency, severity, and duration, has not been well-characterized in the era of highly active antiretroviral therapy (HAART). In this study, we describe the epidemiology of thrombocytopenia using two metho...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2011.582526
更新日期:2011-01-01 00:00:00
abstract::The objective of this study was to obtain pilot data on which to judge the feasibility and sample size needed for a future comparative-effectiveness trial of platelet transfusions in the NICU. We conducted a limited-scope pilot trial in which neonates were randomized to receive platelet transfusions based on platelet ...
journal_title:Platelets
pub_type: 杂志文章,随机对照试验
doi:10.3109/09537104.2013.843072
更新日期:2014-01-01 00:00:00
abstract::The aims of this study were to evaluate the kinetics of platelet counts and mean platelet volume (MPV) in adults with sepsis and to determine whether the responses are infection-specific. This retrospective cohort study included patients admitted to a tertiary-care teaching hospital with microbiologically proven nosoc...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.701027
更新日期:2015-01-01 00:00:00