Abstract:
:The present study had two goals: first, to give a detailed description of a reliable method for full kinetic analysis of serotonin transporter (5HTt) on the membrane of human platelets, and second, as a main issue, to report on physiological influences on kinetic characteristics of this transmembrane transport on a large population of healthy individuals. Full kinetic analyses of platelet serotonin uptake were performed on 334 blood donors of both sexes by the use of 14C-radioisotopic method, which was first optimized according to assumptions of enzyme kinetic analyses, with regard to platelet concentration, duration of uptake, concentration of substrate as well as important technical parameters (underpressure of filtration, blanks, incubating temperature, etc). Kinetic parameters of platelet serotonin uptake in the whole population were for V(max): 142 +/- 25.3 pmol 5HT/10(8) platelets/minute and for K(m): 0.404 +/- 0.089 microM 5HT. Besides the report on kinetic values of 5HT transporter protein, we have also described major physiological influences on the mentioned parameters, V(max), K(m) and their derivative, V(max)/K(m) (transporter efficiency): range and frequency distribution of normal values, intraindividual stability over time, lack of age influence, gender dependence and seasonal variations. The report on kinetic values and main physiological influences on platelet serotonin transport kinetics, obtained by the use of thoroughly reassessed methodology, and on by far the largest human population studied until now, offers a reliable frame of reference for pathophysiological studies of this parameter in various clinical fields.
journal_name
Plateletsjournal_title
Plateletsauthors
Banović M,Bordukalo-Niksić T,Balija M,Cicin-Sain L,Jernej Bdoi
10.3109/09537104.2010.487582subject
Has Abstractpub_date
2010-01-01 00:00:00pages
429-38issue
6eissn
0953-7104issn
1369-1635journal_volume
21pub_type
杂志文章相关文献
PLATELETS文献大全abstract::Type 2B von Willebrand's disease (VWD) is an inherited bleeding disorder characterized by spontaneous binding of large von Willebrand factor (VWF) multimers to platelets in vivo. This phenomenon induces the clearance of both large multimers and platelets, usually resulting in thrombocytopenia with slightly increased p...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100500441150
更新日期:2006-05-01 00:00:00
abstract::The influence of three anti-platelet drugs, cilostazol, aspirin, and tirofiban, was investigated on platelet-leukocyte interaction by flow cytometry. When platelets and leukocytes were pre-incubated with anti-platelet drugs and stimulated by thrombin or collagen, cilostazol was found to inhibit platelet adhesion to mo...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100410001715583
更新日期:2004-08-01 00:00:00
abstract::Thrombocytopenia is classically defined as a platelet count of less than 150 000/µl. Counts from 100 000 to 150 000/µl are considered mildly depressed, 50 000 to 100 000/µl moderately depressed, and less than 50 000/µl severely depressed. Thrombocytopenia occurs in about 10% of pregnant women. Gestational thrombocytop...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.724484
更新日期:2013-01-01 00:00:00
abstract::A pathogen-free and standardized xeno-free supplement of growth media is required for the ex vivo propagation of human cells used as advanced therapeutic medicinal products and for clinical translation in regenerative medicine and cell therapies. Human platelet lysate (HPL) made from therapeutic-grade platelet concent...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1849602
更新日期:2020-11-29 00:00:00
abstract::Severe thrombocytopenia is common in dengue virus (DENV) infections. However, studies focusing on the role of profound thrombocytopenia (PT) (nadir platelet counts ≤ 20 000/mm3) in DENV infections are scarce. This study aims to identify the associated features and outcome of DENV patients with PT. It involves 237 adul...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2017.1293802
更新日期:2017-11-01 00:00:00
abstract::Standardized Ginkgo biloba extract, EGb761, has been shown to possess polyvalent properties, such as anti-oxidation, anti-apoptosis and anti-inflammation. Recently, it has also been proposed to have direct protective effects on mitochondria. The effects of EGb761 make it a potential anti-aging drug. Despite that, the ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537100903511448
更新日期:2010-01-01 00:00:00
abstract::ADP induces platelet aggregation in human whole blood and platelet-rich plasma (PRP). ATP induces aggregation in whole blood only; this involves leukocytes and is mediated by ADP. Here we studied ATP- and ADP-induced aggregation in patients with raised leukocyte counts (mean 46.2x10(3) leukocytes/microl). Platelet agg...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100500063889
更新日期:2005-05-01 00:00:00
abstract::The generation of superoxide anion radicals (O2*-) and the other reactive oxygen species (ROS) was estimated by means of cytochrome c reduction and chemiluminescence, as well in resting blood platelets and in platelets stimulated by thrombin in the presence or absence of some inhibitors of pathways involved in platele...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09533710022149395
更新日期:2002-05-01 00:00:00
abstract::Blood platelets, in analogy to other circulating blood cells, can generate reactive oxygen/nitrogen species (ROS/RNS) that may behave as second messengers and may regulate platelet functions. Accumulating evidence suggest a role of ROS/RNS in platelet activation. On the other hand, an increased production of ROS/RNS c...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537100701504087
更新日期:2007-12-01 00:00:00
abstract::Current guidelines favor dual anti-platelet therapy with ticagrelor 90 mg BID (T90BID) over clopidogrel 75 mg QD (C75QD) in addition to aspirin for acute coronary syndrome. However, an increased risk of ticagrelor-related adverse events prompted the evaluation of low-dose regimens. This study (NCT03381742) retrospecti...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1732328
更新日期:2021-01-02 00:00:00
abstract::The DP receptor-dependence of inhibition of platelet aggregation (PA), degranulation, and TXA(2) synthesis, by PGD(2), in human whole blood, has not been established since selective antagonists have only recently been developed. Accordingly, the effects of PGD(2) (30 nM), were determined using the DP receptor antagoni...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109509078450
更新日期:1995-01-01 00:00:00
abstract::The aims of this study were to evaluate the kinetics of platelet counts and mean platelet volume (MPV) in adults with sepsis and to determine whether the responses are infection-specific. This retrospective cohort study included patients admitted to a tertiary-care teaching hospital with microbiologically proven nosoc...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.701027
更新日期:2015-01-01 00:00:00
abstract::Extracellular proteolysis of platelet plasma membrane proteins is an event that ensues platelet activation. Shedding of surface receptors such as glycoprotein (GP) Ibα, GPV and GPVI as well as externalized proteins P-selectin and CD40L releases soluble ectodomain fragments that are subsequently detectable in plasma. T...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537104.2016.1203401
更新日期:2017-06-01 00:00:00
abstract::Fibrin glue is a promising alternative for low-output enterocutaneous fistula closure. Bacterial flora colonizing inside the fistula tract, however, may limit the glue application. Single-donor-derived, platelet-rich materials were hypothesized in this study to have antimicrobial activity against Gram-negative microor...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.761685
更新日期:2013-01-01 00:00:00
abstract::Platelet-rich fibrin (PRF) is generated from the patients' own venous blood by a single centrifugation step without the additional use of anticoagulants. Based on the previously described LSCC (low-speed centrifugation concept), our group showed that modification of the centrifugation setting, that is, reducing the re...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2018.1445835
更新日期:2019-01-01 00:00:00
abstract::Autophagy is an effective strategy for cell development by recycling cytoplasmic constituents. Genetic deletion of autophagy mediator Atg7 in hematopoietic stem cells (HSCs) can lead to failure of megakaryopoiesis and enhanced autophagy has been implicated in various hematological disorders such as immune thrombocytop...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1204436
更新日期:2017-01-01 00:00:00
abstract::The aim of the study was to evaluate how platelet aggregation relates to left ventricular hypertrophy, presence of transient myocardial ischemia and coronary atherosclerosis in essential hypertensive patients. For that purpose platelet aggregation, symptom-limited treadmill-test, echocardiography and coronary angiogra...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109876672
更新日期:1998-01-01 00:00:00
abstract::Platelets are needed to prevent or arrest bleeding and aggregate at the site of injury upon vascular damage. Platelets express receptors for estrogens which might affect the function of the platelets and their hemostatic ability. The aim was to identify possible differences in platelet function related to age, gender,...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2018.1466387
更新日期:2019-01-01 00:00:00
abstract::Sheehan's syndrome (SS) is an adenopituitary insufficiency caused by hypovolemia secondary to excessive blood loss during or after childbirth. However, the mechanism of postpartum hemorrhage and ischemia is not clear. We aimed to evaluate the bleeding disorders among patients with SS, in comparison with healthy contro...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2010.525722
更新日期:2011-01-01 00:00:00
abstract::Binding of podoplanin to the C-type lectin-like receptor 2 (CLEC-2) promotes platelet activation and soluble CLEC-2 (sCLEC-2) is shed from activated platelets. The role of sCLEC-2 in the plasma is unknown. The expression level and plasma concentration of sCLEC-2 could be affected by variants of the corresponding gene,...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1849601
更新日期:2020-11-29 00:00:00
abstract::Patients with peripheral artery disease (PAD) following peripheral percutaneous transluminal angioplasty (PTA) with stent implantation are prone to stent thrombosis despite treatment with aspirin and clopidogrel. Impaired clopidogrel responsiveness is associated with increased risk of ischemic events in patients follo...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.676220
更新日期:2013-01-01 00:00:00
abstract::The purpose of the study was to evaluate safety, effects on platelet aggregation and pharmacokinetics of F(ab')(2) fragments of anti-glycoprotein (GP) IIb-IIIa murine monoclonal antibody FRaMon (F(ab')(2) FRaMon) upon its intravenous administration in patients undergoing high-risk coronary angioplasty. Patients were t...
journal_title:Platelets
pub_type: 临床试验,杂志文章
doi:10.1080/0953710021000057839
更新日期:2002-12-01 00:00:00
abstract::Postoperative atrial fibrillation (AF) is a common complication of coronary artery bypass grafting (CABG). The mean platelet volume (MPV) is an important marker of platelet activity and is associated with cardiovascular risk factors. We investigated whether the MPV is associated with the development of AF after CABG. ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2013.767443
更新日期:2014-01-01 00:00:00
abstract::Studies on markers for bleeding risk among thrombocytopenic cancer patients are lacking. This prospective observational cohort study investigated whether platelet parameters and a standardised bleeding questionnaire predicted bleeding or prophylactic platelet transfusions in patients with cancer and thrombocytopenia. ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2015.1131814
更新日期:2016-09-01 00:00:00
abstract::Platelets are involved in host defense via clearance of bacteria from the circulation, interaction with virus particles, and uptake of various size particulates. There is a growing interest in micro- and nanoparticles for drug delivery and there is evidence that the properties of these particles critically influence t...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2012.703792
更新日期:2013-01-01 00:00:00
abstract::Intracranial hemorrhage (ICH) is a devastating complication of immune thrombocytopenia (ITP). However, information on ICH in ITP patients under the age of 60 years is limited, and no predictive tools are available in clinical practice. A total of 93 adult patients with ITP who developed ICH before 60 years of age were...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1786042
更新日期:2020-07-02 00:00:00
abstract::Human melanoma cell Line, HMV-I, has been shown to induce platelet aggregation without thrombin generation. The platelet-aggregating activity of HMV-I cells increased with increasing culture periods of the cells. However, data indicate that the platelet-aggregating activity of HMV-I cells is dependent upon the cell de...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109509078456
更新日期:1995-01-01 00:00:00
abstract::Autoimmune thrombocytopenic purpura (ITP) is characterized by an abnormally low platelet count and bleeding risks. The exact triggering event remains elusive. Oxidative stress may play a role in several autoimmune diseases. A direct link between platelets in ITP and oxidative stress has not yet been addressed. The int...
journal_title:Platelets
pub_type: 临床试验,杂志文章
doi:10.3109/09537104.2011.610909
更新日期:2012-01-01 00:00:00
abstract::Platelets, besides their specialised role in haemostasis and atherothrombosis, actively modulate innate and adaptive immune responses with crucial roles in immune surveillance, inflammation and host defence during infection. An important prerequisite for platelet-mediated changes of immune functions involves direct en...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/09537104.2018.1430358
更新日期:2018-11-01 00:00:00
abstract::In the differential diagnosis of primary and secondary thrombocytosis platelet function tests may play an important role. We examined the applicability of a platelet filter test (shear-dependent platelet aggregation) as a tool, to differentiate primary thrombocytosis (cases with myeloproliferative disorders) from seco...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100075788
更新日期:2000-02-01 00:00:00