Abstract：
BACKGROUND:Studies in platelet of 5-HT uptake transporters have been performed using binding assay methodology designed for ligand-receptor interactions; however, uptake transporters present requirements that may question the validity of these particular binding assays. METHODS:To explore methodologic aspects that may be crucial to the validity of these assays, we studied the binding of [3H]-paroxetine to platelet membranes of healthy subjects under different conditions of time, temperature, and protein concentrations. RESULTS:A correlation between protein concentration in incubation media and percentage of specific binding of [3H]-paroxetine was found: the lower the protein concentrations (10 and 20 microg/mL) in incubation media, the lower the percentage of specific [3H]-paroxetine binding. Moreover, low specificity in [3H]-paroxetine binding affected Bmax values obtained in saturation binding experiments. CONCLUSIONS:The use of low protein concentrations could affect Bmax values in binding assays of 5-HT uptake transporters. This may induce confusing interpretation of data in clinical experiments that use human platelets to explore the participation of serotonin in depressed patients.
journal_name
Arch Med Resjournal_title
Archives of medical researchauthors
Jurado N,Torner C,Heinze G,López G,Mendoza-Sotelo J,Lazo-Langner A,Moreno Jdoi
10.1016/j.arcmed.2003.03.001subject
Has Abstractpub_date
2003-09-01 00:00:00pages
422-7issue
5eissn
0188-4409issn
1873-5487pii
S0188-4409(03)00094-8journal_volume
34pub_type
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