Abstract:
BACKGROUND:Because lymphocytes from fragile X patients have been reported as hypersensitive to bleomycin-induced chromatid breaks and because the number of trinucleotide repeats in families with fragile X syndrome has a propensity to expand, we have investigated the possibility that fragile X cells may be hypersensitive to DNA damage and have a lower capacity for DNA repair. METHODS:Lymphocytes from normal and fragile X syndrome donors were immortalized by Epstein-Barr virus transformation. Characteristics of fragile X syndrome including the folate-sensitive fragile site on chromosome Xq27.3, length of CGG repeat expansion, and FMRP expression in Epstein-Barr virus-transformed lymphoblastoid cell lines were analyzed by standard cytogenetic methods, Southern blot, and Western blot, respectively. Analysis of DNA damage and repair induced by hydrogen peroxide, bleomycin, ethyl methanesulfonate, 4-nitroquinoline-N-oxide, etoposide, and mitomycin C was carried out by single-cell gel electrophoresis assay (known as comet assay). RESULTS:Lymphoblastoid cell lines from fragile X donors had a folate-sensitive fragile site on chromosome Xq27.3, no or low FMRP expression, and expansion of the CGG repeat. Results of comet assay showed that fragile X cells were not more sensitive to mutagen-induced DNA strand breaks and did not have lower DNA repair capacity in comparison with normal cells. Furthermore, one fragile X cell line showed hyposensitivity to DNA strand breaks induced by hydrogen peroxide, bleomycin, and ethyl methansulfonate. CONCLUSIONS:The results of this study do not support the notion that CGG trinucleotide expansion in fragile X syndrome is caused by permanent deficiency in DNA repair.
journal_name
Arch Med Resjournal_title
Archives of medical researchauthors
Wang TS,Hsieh LJ,Hsu TY,Chung CH,Li SYdoi
10.1016/s0188-4409(01)00376-9subject
Has Abstractpub_date
2002-03-01 00:00:00pages
128-35issue
2eissn
0188-4409issn
1873-5487pii
S0188-4409(01)00376-9journal_volume
33pub_type
杂志文章abstract:BACKGROUND:The diuretic response has been shown to be a robust independent marker of cardiovascular outcomes in acute heart failure (ADHF) patients. The objectives of this clinical research, will aim are to: a) include diuresis in the formula for diuretic response (R-to-D); b) add to R-to-D the value of a pre-discharge...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2018.08.007
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Myocardial infarction (MI) is one of the most important diseases that has stimulated interest in understanding cardiac function recovery. SDF-1 is a chemotactic factor and a pro-angiogenic molecule; SDF-1 degradation is inhibited by dipeptidyl peptidase-4 (DPP4) inhibitors, such as vildagliptin. We investiga...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2019.07.004
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Quinolone-induced mutagenesis in the Salmonella typhimurium hisG48 strains suggests that these antibiotics are oxygen free radical generators. The use of beta-carotene as antioxidant was evaluated as an alternative to reduce oxidative cell damage in patients who need therapy with nalidixic acid, norfloxacin,...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/s0188-4409(00)00046-1
更新日期:2000-03-01 00:00:00
abstract::With the use of the polymerase chain reaction (PCR), we investigated the frequency of the typical and atypical genes of the alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci, in a group of patients with alcoholic liver cirrhosis and two comparison groups. Contrary to what has been found in the J...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:Growth deficits are common in children with sickle cell disease. Few prospective studies are available and the pathophysiologic basis for the impaired growth is not clearly understood. Our objectives were to collect data on anthropomorphic measurements of children with sickle cell disease prospectively follo...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/s0188-4409(01)00360-5
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND:Previous studies have reported vitamin D receptor (VDR) polymorphisms in multiple sclerosis (MS); however, the results remain contradictory. This study aimed to investigate the association between VDR polymorphisms and the risk of MS. METHODS:PubMed and Embase databases were searched to obtain eligible stud...
journal_title:Archives of medical research
pub_type: 杂志文章,meta分析
doi:10.1016/j.arcmed.2019.10.007
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:It is well documented that Giardia duodenalis undergoes surface antigenic variation both in vivo and in vitro. Proteins involved have been characterized and referred to as VSP (variable surface protein). METHODS:Two cloned cDNA inserts of 0.45 and 1.95 kb were obtained from G. duodenalis expression library ...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2003.12.005
更新日期:2004-05-01 00:00:00
abstract::Reports on simultaneous central and peripheral nervous system involvement in a patient with brucellosis are very rare. We report of one young female patient with a long history of consumption of non-pasteurized dairy products in which clinical and laboratory findings confirmed the existence of an active brucellosis wi...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract:BACKGROUND AND AIMS:Although sporadic cases of cancer in patients with fragile X syndrome (FXS) have been reported, extensive studies carried out in Denmark and Finland concluded that cancer incidence in these patients is lower than in the general population. On the other hand, the FMR1 protein, which is involved in th...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2010.03.001
更新日期:2010-02-01 00:00:00
abstract:BACKGROUND:Several clinical conditions suggest an effect of sex steroids on glucose homeostasis in women. Studies examining this phenomenon have yielded controversial results. METHODS:To investigate the effect of the menstrual cycle on insulin sensitivity, glucose effectiveness and acute insulin response to glucose us...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/s0188-0128(98)00008-6
更新日期:1999-01-01 00:00:00
abstract::The local induction of humoral immune response to Entamoeba histolytica trophozoites in the gut has not been studied. This work reports some of our recent studies aimed to induce optimal immune responses against E. histolytica in mice and to describe a novel approach for monitoring mucosal immune responses induced in ...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:Acute myocardial infarction (AMI) is a leading cause of death in Mexico. Atherogenic lipid profile is a key component in AMI. Thus, it is imperative to find drug therapies able to reduce atherogenic lipids in AMI patients and prevent subsequent myocardial infarctions. AIM OF THE STUDY:To investigate the eff...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2019.08.002
更新日期:2019-05-01 00:00:00
abstract::Trichomonas vaginalis cytoadherence is mediated by four adhesins (AP65, AP51, AP33 and AP23). Adhesin gene expression was previously shown to be up-regulated by iron. Therefore, a cDNA library was constructed from mRNA of T. vaginalis grown in a high-iron medium. Ten cDNA clones (three for AP65, one for AP51, and six ...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:The benefits of a special stroke care unit (SSCU) over a non-specialized stroke care unit (NSSCU) is well documented in the literature. However, there are concerns that the benefits seen in the SSCU do not consider clinical risk factors that affect thrombolysis therapy. METHOD:Retrospective data were collec...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2019.01.002
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND AND AIMS:Helicobacter pylori is the most common etiological factor of chronic infection worldwide. It has also been found in human dental plaques, mouth, saliva, tonsils and adenoid tissue, medial ear or nasal polyps and sinuses mucosa, as well in several benign and malignant lesions of the larynx and pharyn...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2011.12.005
更新日期:2011-11-01 00:00:00
abstract::A definitive single hypothesis about HIV pathogenesis is still lacking in specialized literature. Rather, a picture of the extreme complexity in AIDS causality ensues from the extensive information available. This article contains an outline of the most relevant data on the subject of AIDS pathogenesis, organized into...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:
更新日期:1996-07-01 00:00:00
abstract::Blood-brain barrier (BBB) disruption has been associated with several acute and chronic brain disorders such as Alzheimer's disease, Parkinson's disease and epilepsy. This represents a critical situation because damaged integrity of the BBB is related to the influx of immune mediators, plasma proteins and other outsid...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:10.1016/j.arcmed.2014.11.010
更新日期:2014-11-01 00:00:00
abstract::Glutamate neurotransmitter action on postsynaptic receptors is terminated by its clearance from the synaptic cleft by transporter proteins located in neurons and glial cells. Failure of glutamate removal can lead to neuronal death due to its well-known neurotoxic properties. Glutamate transporters are dependent on ext...
journal_title:Archives of medical research
pub_type: 杂志文章,评审
doi:10.1016/j.arcmed.2005.05.014
更新日期:2006-01-01 00:00:00
abstract::Systems biology and the Herbalome Project have good prospects if the specific episteme-method approach of traditional Chinese medicine (TCM) is taken into serious consideration. TCM takes advantage of a specific holistic method, which is based on the episteme method approach derived from the conceptions of unceasing m...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2008.09.006
更新日期:2009-01-01 00:00:00
abstract::COVID-19 is a devastating global pandemic around the world. While the majority of infected cases appear mild, in some cases individuals present respiratory complications with possible serious lung damage. There are no specific treatments for COVID-19 as yet, though a number are under evaluation, including experimental...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2020.04.018
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:The present study was designed to examine whether resveratrol, a potent antioxidant, protects against renal ischemia-reperfusion (I/R) injury. METHODS:Wistar albino rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 6 h of reperfusion. Resveratrol (RVT, 30 m...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2006.04.003
更新日期:2006-10-01 00:00:00
abstract:BACKGROUND AND AIMS:Previous published data have implicated TP53 codon 72 polymorphisms as risk factors for various cancers. Growing bodies of studies have been conducted on the association of TP53 codon 72 polymorphisms with susceptibility to oral carcinoma and have yielded inconclusive results. The aim of the present...
journal_title:Archives of medical research
pub_type: 杂志文章,meta分析
doi:10.1016/j.arcmed.2009.09.004
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:This investigation was undertaken to study the clinical characteristics and the humoral immune pattern in lymphedema patients undergoing O-beta-hydroxy-ethyl rutoside therapy. METHODS:A complete physical examination was performed on 27 lymphedema patients (postmastectomy and postphlebitis) and 17 healthy co...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/s0188-4409(00)00272-1
更新日期:2001-03-01 00:00:00
abstract:BACKGROUND:Psoriasis is a chronic inflammatory skin disease in which several Th1 cytokines such as interleukin-1 beta (IL-beta) have been shown to play a pivotal role. Psychological stress has also been implicated in triggering or exacerbating the disease. METHODS:Salivary IL-1beta and cortisol levels of 25 patients w...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2006.09.009
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND:Vascular endothelial growth factor (VEGF), an important regulator of angiogenesis and vascular permeability, is involved in various steps of many malignancies. Gene polymorphisms within the gene encoding VEGF have been shown to be independently associated with an adverse outcome in various malignancies inclu...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2009.07.011
更新日期:2009-10-01 00:00:00
abstract::Immunity in leishmaniasis is mediated by T cells, but protective responses in humans have not been fully defined. In this study, the functional activity of CD4+ T cell clones derived from an immune individual was investigated to identify potentially protective responses. The T cells proliferated and produced interfero...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:This study aims to investigate the efficacy of fluoxetine and paroxetine on the levels of depression-anxiety, quality of life, disability, and metabolic control in type II diabetes mellitus (DM) patients. METHODS:The patients were first applied the Hospital Anxiety-Depression Scale (HADS). After a psychiatr...
journal_title:Archives of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.arcmed.2004.12.018
更新日期:2005-03-01 00:00:00
abstract::One of the mechanisms that Entamoeba histolytica uses to evade host immune response is inhibition of the polymorphonuclear (PMN) leukocyte respiratory burst. In studies previously conducted in a model used in our laboratory, we observed that when treating trophozoites with different zinc concentrations certain amebic ...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2004.10.005
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND AND AIMS:An increasing number of studies have been published concerning meeting targets of clinical guidelines for different aspects of the diagnosis and treatment of patients with end-stage renal disease. Most of these studies have shown that guideline recommendations are not always satisfied, and results o...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2013.01.008
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:Oxidative and endoplasmic reticulum stresses contribute to the pathogenesis of β-cell dysfunction in diabetes mellitus. This study investigates the effect of isopulegol on the above stresses in HFD/STZ induced diabetic rats. METHODS:Animals in group I and II were placed in normal pellet diet and group II wa...
journal_title:Archives of medical research
pub_type: 杂志文章
doi:10.1016/j.arcmed.2020.02.001
更新日期:2020-04-01 00:00:00