Abstract：
BACKGROUND AND AIMS:Bacteremia is a common serious manifestation of disease in the intensive care unit (ICU), which requires quick and accurate determinations of pathogens to select the appropriate antibiotic treatment. To overcome the shortcomings of traditional bacterial culture (BC), we have adapted next-generation sequencing (NGS) technology to identify pathogens from cell-free plasma DNA. METHODS:In this study, 78 plasma samples from ICU patients were analyzed by both NGS and BC methods and verified by PCR amplification/Sanger sequencing and ten plasma samples from healthy volunteers were analyzed by NGS as negative controls to define or calibrate the threshold of the NGS methodology. RESULTS:Overall, 1578 suspected patient samples were found to contain bacteria or fungi by NGS, whereas ten patients were diagnosed by BC. Seven samples were diagnosed with bacterial or fungal infection both by NGS and BC. Among them, two samples were diagnosed with two types of bacteria by NGS, whereas one sample was diagnosed with two types of bacteria by BC, which increased the detectability of bacteria or fungi from 11 with BC to 17 with NGS. Most interestingly, 14 specimens were also diagnosed with viral infection by NGS. The overall diagnostic sensitivity was significantly increased from 12.82% (10/78) by BC alone to 30.77% (24/78) by NGS alone for ICU patients, which provides more useful information for establishing patient treatment plans. CONCLUSION:NGS technology can be applied to detect bacteria in clinical blood samples as an emerging diagnostic tool rich in information to determine the appropriate treatment of septic patients.
journal_name
Arch Med Resjournal_title
Archives of medical researchauthors
Long Y,Zhang Y,Gong Y,Sun R,Su L,Lin X,Shen A,Zhou J,Caiji Z,Wang X,Li D,Wu H,Tan Hdoi
10.1016/j.arcmed.2016.08.004subject
Has Abstractpub_date
2016-07-01 00:00:00pages
365-371issue
5eissn
0188-4409issn
1873-5487pii
S0188-4409(16)30109-6journal_volume
47pub_type
杂志文章abstract：:Mongolian gerbils were inoculated intracecally with E. histolytica trophozoites cultured monoxenically. During the first hours of interaction an increase in mucus production was observed. Microulcerative mucosal lesions appeared 24 to 72 h post-inoculation. Inflammatory infiltrate and edema of the lamina propria were ...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:A prospective study to evaluate the role of vitamin C and cervico-vaginal infection in the premature rupture of amniotic membranes (PROM) was designed. The leukocyte vitamin C levels of 44 pregnant women that did not consume vitamin supplements was evaluated at weeks 20, 28 and 32 of pregnancy. On each evaluation the ...
journal_title：Archives of medical research
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doi：
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND:During congestive heart failure, desensitization of beta-adrenoceptors is related to a lower adrenergic responsiveness in the heart; little is known about alpha 1-adrenoceptors in the vasculature under this condition. We evaluated alpha 1D-adrenoceptor response in aorta and carotid arteries in a model of con...
journal_title：Archives of medical research
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doi：10.1016/s0188-0128(99)00033-0
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abstract：BACKGROUND AND AIMS:The use of automated peritoneal dialysis (APD) is increasing compared to continuous ambulatory peritoneal dialysis (CAPD). Surprisingly, little data about health benefits and cost of APD exist, and virtually no information comparing the cost-utility between CAPD and APD is available. We undertook th...
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更新日期：1992-04-01 00:00:00
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journal_title：Archives of medical research
pub_type： 杂志文章,评审
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journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：BACKGROUND:Associations between hyperuricemia, metabolic syndrome, cardiovascular disease and diabetes have been reported. Limited information, however, is available concerning the prevalence and correlates of hyperuricemia among Thai men and women. We sought to estimate the prevalence of hyperuricemia among a populati...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2006.03.008
更新日期：2006-10-01 00:00:00
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journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:Antiepileptics including benzodiazepines have long been recognized to provoke seizures and precipitate status epilepticus occasionally. This has a special clinical importance in the case of diazepam because of its use as first choice medication in its management. This report is intended to highlight the clin...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/s0188-4409(00)00043-6
更新日期：2000-01-01 00:00:00
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journal_title：Archives of medical research
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更新日期：2015-05-01 00:00:00
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journal_title：Archives of medical research
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更新日期：2018-11-01 00:00:00
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更新日期：2020-10-01 00:00:00
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journal_title：Archives of medical research
pub_type： 杂志文章,评审
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abstract：BACKGROUND AND AIMS:To explore hepatoprotective action of curcumin (CMN, a bioflavonoid) on oxaliplatin (Oxa)-triggered mitochondrial oxidative stress and respiratory chain complexes in liver of rats. Oxa is a ubiquitously utilized platinum-based chemotherapeutic agent commonly used for the treatment of colorectal canc...
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doi：10.1016/j.arcmed.2005.04.015
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journal_title：Archives of medical research
pub_type： 杂志文章,评审
doi：10.1016/j.arcmed.2007.04.006
更新日期：2007-10-01 00:00:00
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doi：10.1016/j.arcmed.2020.12.003
更新日期：2020-12-27 00:00:00
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doi：10.1016/j.arcmed.2010.10.005
更新日期：2010-10-01 00:00:00
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journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2015.07.007
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journal_title：Archives of medical research
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doi：10.1016/j.arcmed.2020.02.001
更新日期：2020-04-01 00:00:00
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journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The myosin heavy chain gene A, mhcA, from E. histolytica was identified by polymerase chain reaction. There is only one copy of this gene on the ameba genome. The mhcA gene encodes a 6 kb messenger RNA. The predicted protein sequence is homologous to myosin II from both, Dictyostelium discoideum and Acanthamoeba caste...
journal_title：Archives of medical research
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doi：
更新日期：1992-01-01 00:00:00
abstract：:Classical descriptions of the pathology of amebiasis portray the parasite as the cause of tissue damage and destruction, and in recent years a number of amebic molecules have been identified as virulence factors. In this review we describe a series of experiments that suggest a more complex host-parasite relation, at ...
journal_title：Archives of medical research
pub_type： 杂志文章,评审
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更新日期：2006-02-01 00:00:00
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journal_title：Archives of medical research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND AND AIMS:In this study, we aimed to investigate whether 1800 MHz frequency electromagnetic radiation (EMR) has an effect on bone healing. METHODS:A total of 30 Wistar albino rats were divided into two equal groups. Fractures were created in the right tibias of all rats; next, intramedullary fixations with K...
journal_title：Archives of medical research
pub_type： 杂志文章
doi：10.1016/j.arcmed.2014.01.006
更新日期：2014-02-01 00:00:00
