Abstract:
:We have established a highly sensitive and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method to detect axillary lymph node metastases of breast cancer. Amplifying cytokeratin 19 (CK19) mRNA transcripts using real-time TaqMan PCR made it possible to quantify axillary metastatic burden. Metastases in 358 axillary lymph nodes obtained from 23 breast cancers of 22 patients were investigated by conventional haematoxylin and eosin (H&E) staining, immunohistochemical staining and quantitative RT-PCR assay. The detection rates of axillary lymph node metastasis using H&E staining, immunohistochemistry and RT-PCR assay were 4.5, 5.9 and 13.1%, respectively. RT-PCR assay was the most sensitive of these three methods for detecting lymph node metastases. Cytokeratin 19 mRNA expression values of both histologically and immunohistochemically positive lymph nodes were significantly higher than the values for lymph nodes judged to be negative by both histological and immunohistochemical methods (P<0.0001), and those of histologically negative, but immunohistochemically positive lymph nodes were significantly higher than the values for lymph nodes judged to be negative by both histological and immunohistochemical methods (P<0.0001). Furthermore, metastatic rates of sentinel nodes were higher than the rates of nonsentinel lymph nodes as measured by all three methods. These results indicate that quantitative RT-PCR assay is a sensitive and reliable method for detecting lymph node metastasis. Furthermore, quantification of metastases in sentinel lymph nodes by quantitative RT-PCR assay may be useful to assess the entire axillary burden of breast cancer patients.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Inokuchi M,Ninomiya I,Tsugawa K,Terada I,Miwa Kdoi
10.1038/sj.bjc.6601248subject
Has Abstractpub_date
2003-11-03 00:00:00pages
1750-6issue
9eissn
0007-0920issn
1532-1827pii
6601248journal_volume
89pub_type
杂志文章abstract::An increasing body of evidence indicates that glutathione S-transferases play a role in the intrinsic and acquired resistance of tumours to anticancer drugs. In view of the wide use of tumour cell lines to understand the factors which confer either sensitivity or resistance to chemotherapeutic agents we have determine...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1989.280
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND:Our previous study demonstrated that extracellular adenosine 5'-triphosphate (ATP) stimulated prostate cancer cell invasion via P2Y receptors. However, the purinergic receptor subtype(s) involved in this process remains unclear. Here we aimed to determine whether P2Y2, one subtype of P2Y receptors, was invol...
journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:2013-09-17 00:00:00
abstract:BACKGROUND:Primary high-risk human papillomavirus (hrHPV) testing in cervical cancer screening shows relatively low specificity, which makes triage testing necessary. In this study, DNA methylation analysis was compared with cytology for triage testing in hrHPV-positive women. Moreover, feasibility of DNA methylation a...
journal_title:British journal of cancer
pub_type: 杂志文章
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1982.97
更新日期:1982-04-01 00:00:00
abstract::Findings in humans and animal models suggest that in utero hormonal and dietary exposures increase later breast cancer risk. Since alcohol intake by adult women consistently increases their breast cancer risk, we wondered whether maternal alcohol consumption during pregnancy increases female offspring's mammary tumori...
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6601793
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:Children with cancer are frequently immunocompromised. While children are generally thought to be at less risk of severe SARS-CoV-2 infection than adults, comprehensive population-based evidence for the risk in children with cancer is unavailable. We aimed to produce evidence of the incidence and outcomes fr...
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journal_title:British journal of cancer
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更新日期:2001-02-02 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1980.201
更新日期:1980-07-01 00:00:00
abstract::Tamoxifen is the current therapy of choice for patients with oestrogen receptor positive breast cancer, and it is currently under evaluation as a prophylactic for women at high risk of developing the disease. However, tamoxifen is also known to induce proliferative changes in the endometrium increasing the risk of dev...
journal_title:British journal of cancer
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doi:10.1038/sj.bjc.6600157
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journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2015-06-09 00:00:00
abstract::We compared the long-term impact of 1- and 2-year screening mammography intervals using prognostic, screening, and outcome information for women aged 50-74 years obtained from the Screening Mammography Program of British Columbia in two time periods, prior to 1997 (policy of annual mammography) and after 1997 (biennia...
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doi:10.1038/sj.bjc.6602393
更新日期:2005-03-14 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.512
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abstract:BACKGROUND:Interferon (IFN)-based therapies could eradicate hepatitis C (HCV) and reduce the risk of hepatocellular carcinoma (HCC). However, HCC could still happen after sustained virological response (SVR). We aimed to develop a simple scoring system to predict the risk of HCC development among HCV patients after ant...
journal_title:British journal of cancer
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更新日期:2013-10-29 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1979.228
更新日期:1979-10-01 00:00:00
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更新日期:2008-11-18 00:00:00
abstract::Laminin-332 is major component of epithelial basement membrane, and has an important role in cell migration and tumour invasion. Recently, the phosphatidylinositol 3-kinase (PI3K) activation induced by laminin-332 during carcinogenesis or tumour invasion has been highlighted in skin squamous cell carcinoma. The expres...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604252
更新日期:2008-03-11 00:00:00
abstract::Detailed serological studies have been undertaken in a small group of cancer patients receiving nonspecific immunotherapy with Corynebacterium parvum (C. parvum). These patients included 4 cases of recurrent malignant melanoma, 2 of stomach cancer and 2 of recurrent breast cancer. They all received an initial i.v. inf...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1975.229
更新日期:1975-09-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2005-09-19 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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journal_title:British journal of cancer
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abstract::The association between chromosomal radiosensitivity and genetic predisposition to head and neck cancer was investigated in this study. In all, 101 head and neck cancer patients and 75 healthy control individuals were included in the study. The G(2) assay was used to measure chromosomal radiosensitivity. The results d...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604345
更新日期:2008-05-20 00:00:00