Abstract:
BACKGROUND:Primary high-risk human papillomavirus (hrHPV) testing in cervical cancer screening shows relatively low specificity, which makes triage testing necessary. In this study, DNA methylation analysis was compared with cytology for triage testing in hrHPV-positive women. Moreover, feasibility of DNA methylation analysis directly on brush-based self-sampled specimens was assessed. METHODS:Non-responding women from population-based screening were invited to self-collect a cervico-vaginal specimen for hrHPV testing; hrHPV-positive women were referred to a physician for triage liquid-based cytology. DNA methylation analysis was performed on 128 hrHPV-positive physician-collected triage samples and 50 matched brush self-samples with QMSP for C13ORF18, EPB41L3, JAM3 and TERT. RESULTS:In physician-taken triage material, DNA methylation analysis of JAM3 showed the highest combined specificity (88%) and sensitivity (82%) for detection of CIN3+, whereas cytology showed a specificity of 48% and a sensitivity of 91%. Out of 39 women with abnormal cytology and normal histology (false-positive by cytology), 87% were negative for JAM3 and 90% for C13ORF18 methylation. Agreement between DNA methylation analysis performed directly on the matched self-sampled material and physician-taken samples was 88% for JAM3 (κ=0.75, P<0.001) and 90% for C13ORF18 (κ=0.77; P<0.001). CONCLUSIONS:DNA methylation analysis as a triage test in hrHPV-positive women is an attractive alternative to cytology. Furthermore, DNA methylation is feasible directly on brush-based self-samplers and showed good correlation with matched physician-taken samples. Direct molecular triage on self-collected specimens could optimise the screening program, especially for non-responders, as this would eliminate the need for an additional physician-taken scraping for triage testing.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Boers A,Bosgraaf RP,van Leeuwen RW,Schuuring E,Heideman DA,Massuger LF,Verhoef VM,Bulten J,Melchers WJ,van der Zee AG,Bekkers RL,Wisman GBdoi
10.1038/bjc.2014.392subject
Has Abstractpub_date
2014-09-09 00:00:00pages
1095-101issue
6eissn
0007-0920issn
1532-1827pii
bjc2014392journal_volume
111pub_type
杂志文章abstract::Van Trommel et al have previously shown that serum human chorionic gonadotropin (hCG) cutoff levels can provide early prediction of resistance to first-line methotrexate (MTX) in patients with persistent trophoblastic disease (PTD). In this study, we validate this approach of prediction of resistance to single-agent c...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604849
更新日期:2009-03-24 00:00:00
abstract:BACKGROUND:Twenty to 40% localised RCC patients may experience recurrence after curative surgery. Limited miRNA predictors have been identified for ccRCC recurrence. METHODS:Through a multi-phase study design, we analysed miRNAs in tissues obtained from 203 ccRCC patients. Paired t-test was used for tumour-normal comp...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.392
更新日期:2017-01-03 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.23
更新日期:1998-01-01 00:00:00
abstract:OBJECTIVE:Detection of lymph node involvement in women with IB2-IIB cervical cancer could have a positive effect on survival. We set out to evaluate the incidence of pelvic and/or para-aortic lymph node involvement using the sentinel node (SN) biopsy and its impact on survival. METHODS:From 2002 to 2010, 66 women with...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.541
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abstract::In compiling official mortality statistics, rules for selecting the underlying cause of death have changed twice in the last 20 years in England. Mortality statistics for most types of cancer were not greatly affected, but there were significant effects on coding for cancers of colon, liver, breast, prostate, testis a...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601633
更新日期:2004-03-08 00:00:00
abstract:BACKGROUND:To quantify the benefits (cancer prevention and down-staging) and harms (recall and excess treatment) of cervical screening starting from age 20 years rather than from age 25 years. METHODS:We use routine screening and cancer incidence statistics from Wales (for screening from age 20 years) and England (scr...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2014.65
更新日期:2014-04-02 00:00:00
abstract::A comparative study has been made of fibroblasts obtained from patients with differing susceptibilities to malignant disease, both with respect to their chromosome complements and their transformation with SV40 virus. Fibroblasts from 2 Bloom's syndrome patients were found not to have raised SV40 transformation rates ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1977.235
更新日期:1977-11-01 00:00:00
abstract::The brain-specific angiogenesis inhibitor 1 gene has been isolated in an attempt to find fragments with p53 "functional" binding sites. As reported herein and by others, brain-specific angiogenesis inhibitor 1 expression is present in some normal tissues, but is reduced or lost in tumour tissues. Such data and its par...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600067
更新日期:2002-02-01 00:00:00
abstract::Currently the only tumour marker to have a well-defined and validated role in the management of ovarian cancer is CA125. Changes in the level of CA125 can be used as a reliable indication of response or progression according to various criteria, but it does not yet have a clear place in diagnosis or prognosis. Its val...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1977.156
更新日期:1977-07-01 00:00:00
abstract::Cisplatin is one of the most active cytotoxic agents in the treatment of testicular cancer, but its clinical use is associated with side-effects such as ototoxicity, neurotoxicity and nephrotoxicity. Long-term kidney damage from cisplatin particularly affects the proximal tubular apparatus and can be detected by incre...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.673
更新日期:1996-12-01 00:00:00
abstract:BACKGROUND:Epidermal growth factor receptor (EGFR) is highly expressed in adenoid cystic carcinoma (ACC). The efficacy and toxicity of cetuximab with concomitant platinum-based chemoradio- or chemotherapy in patients with locally advanced or metastatic ACC, respectively, was evaluated. METHODS:Eligible patients (9 wit...
journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:2013-09-03 00:00:00
abstract:BACKGROUND:The arachidonic acid metabolite 12(S)-HETE is suspected to enhance metastatic spread by inducing cancer cell- and lymph endothelial cell (LEC) motility. However, the molecular mechanisms leading to 12(S)-HETE-triggered cell migration are still elusive. METHODS:To delineate the signalling pathways involved i...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.201
更新日期:2016-07-26 00:00:00
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journal_title:British journal of cancer
pub_type: 已发布勘误
doi:10.1038/s41416-020-0879-6
更新日期:2020-08-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690313
更新日期:1999-04-01 00:00:00
abstract::The mature epidermal growth factor receptor (EGFR) neither associates with nor requires the molecular chaperone heat-shock protein 90 (Hsp90). Mutations in EGFR exons 18, 19, and 21 confer Hsp90 chaperone dependence. In non-small cell lung cancer (NSCLC), these mutations are associated with enhanced sensitivity to EGF...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603950
更新日期:2007-09-17 00:00:00
abstract::Lung cancer is the primary cause of cancer mortality in developed countries. First diagnosis only when disease has already reached the metastatic phase is the main reason for failure in treatment. To this regard, although low-dose spiral computed tomography (CT) has proven to be effective in the early detection of lun...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6605660
更新日期:2010-06-08 00:00:00
abstract::The aim of this study was to determine the dose-limiting toxicity (DLT) and establish the recommended dose for mitomycin C added every 3 weeks to the standard combination dose of capecitabine. Cohorts of at least three patients with pretreated gastrointestinal carcinoma received capecitabine 1000 mg m(-2) orally twice...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/sj.bjc.6602025
更新日期:2004-08-31 00:00:00
abstract:BACKGROUND:Cellular Dissociation Grade (CDG) composed of tumour budding and cell nest size has been shown to independently predict prognosis in pre-therapeutic biopsies and primary resections of oesophageal squamous cell carcinoma (ESCC). Here, we aimed to evaluate the prognostic impact of CDG in ESCC after neoadjuvant...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-019-0623-2
更新日期:2019-12-01 00:00:00
abstract::DepoCyte is a slow-release formulation of cytarabine designed for intrathecal administration. The goal of this multi-centre cohort study was to determine the safety and efficacy of DepoCyte for the intrathecal treatment of neoplastic meningitis due to breast cancer. DepoCyte 50 mg was injected once every 2 weeks for o...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1054/bjoc.2000.1574
更新日期:2001-01-01 00:00:00
abstract::Microsatellite instability is present in over 80% of the hereditary non-polyposis colorectal carcinoma and about 15-20% of the sporadic cancer. Microsatellite instability is caused by the inactivation of the mismatch repair genes, such as primarily hMLH1, hMSH2. To study the mechanisms of the inactivation of mismatch ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600148
更新日期:2002-02-12 00:00:00
abstract::The aim of this study was to assess the cost-effectiveness of combination chemotherapy with paclitaxel/cisplatin, compared with standard etoposide/cisplatin in patients with advanced non-small cell lung cancer (NSCLC). We obtained the primary survival and resource utilization data from a large three-arm randomized tri...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-05-01 00:00:00
abstract::Advances in understanding the role of vascular endothelial growth factor (VEGF) in normal physiology are giving insight into the basis of adverse effects attributed to the use of VEGF inhibitors in clinical oncology. These effects are typically downstream consequences of suppression of cellular signalling pathways imp...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6603813
更新日期:2007-06-18 00:00:00
abstract::Renal tumours were induced in dietary-primed rats by injection of dimethylnitrosamine. Control and tumour tissue was excised at varying periods and maintained in short-term organ culture in the presence of 3H- or 14C-fucose. The plasma membranes were then isolated, and the isotopic profiles of normal kidney and renal ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1978.106
更新日期:1978-05-01 00:00:00
abstract::Tamoxifen is the current therapy of choice for patients with oestrogen receptor positive breast cancer, and it is currently under evaluation as a prophylactic for women at high risk of developing the disease. However, tamoxifen is also known to induce proliferative changes in the endometrium increasing the risk of dev...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600157
更新日期:2002-03-04 00:00:00
abstract::Previous studies have demonstrated that BR-931, a hepatic peroxisome proliferator, can induce liver tumours in mice and rats. Since alterations in gene expression may play a critical role in multistage hepatocarcinogenesis, the present studies examined the expression of the c-myc, c-H-ras, epidermal growth factor (EGF...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.406
更新日期:1991-11-01 00:00:00
abstract::The influence of human serum albumin (HA) on the biological effects of 13 chemotherapeutic agents was studied in vitro in the human leukaemia cell line MOLT-3. On the basis of changes in biological activity influenced by HA, these drugs may be divided into three types. Type I agents include cis-diamminedichloroplatinu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1980.103
更新日期:1980-04-01 00:00:00
abstract::Three hundred and ninety women participated in the quality of life (QL) study of ACCOG1, a high-dose vs conventional adjuvant chemotherapy breast cancer trial, for patients with a high risk of relapse. Patients completed the European Organisation for Research and Treatment of Cancer QLQ-C30, questions on menopausal sy...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603454
更新日期:2006-12-18 00:00:00
abstract::The cytochrome P450 CYP1B1 is consistently overexpressed in tumour cells as compared to their normal counterparts, but its precise role in drug resistance is yet to be defined. It has been reported that transfection of CYP1B1 results in increased resistance to docetaxel in V79 cells (McFadyen et al, 2001). In this stu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604195
更新日期:2008-02-12 00:00:00
abstract:METHODS:Maternally reported congenital abnormalities (CAs) were examined in a case-control study of 278 cases of paediatric germ cell tumours (GCTs) and 423 controls. RESULTS AND CONCLUSIONS:Germ cell tumours were significantly associated with cryptorchidism in males (OR=10.8, 95% CI: 2.1-55.1), but not with any other...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605169
更新日期:2009-08-04 00:00:00