ASTRO-FOLD: a combinatorial and global optimization framework for Ab initio prediction of three-dimensional structures of proteins from the amino acid sequence.

abstract：:The dependences of the steady-state critical concentration and average filament length of actin solutions, on the filament branching and capping rates, are calculated using a rate methodology based on the total number of actin filaments. The methodology generalizes calculations of the "treadmilling" actin concentratio...

journal_title：Biophysical journal

authors： Carlsson AE

更新日期：2005-07-01 00:00:00

abstract：:A recent theory for the long time dynamics of flexible chain molecules is applied for the first time to a peptide of biological importance, the neurotransmitter met-enkephalin. The dynamics of met-enkephalin is considerably more complicated than that of the previously studied glycine oligomers; met-enkephalin contains...

journal_title：Biophysical journal

authors： Kostov KS,Freed KF

更新日期：1999-01-01 00:00:00

abstract：:The atomic force microscopy (AFM) indentation method combined with the brush model can be used to separate the mechanical response of the cell body from deformation of the pericellular layer surrounding biological cells. Although self-consistency of the brush model to derive the elastic modulus of the cell body has be...

journal_title：Biophysical journal

authors： Dokukin M,Ablaeva Y,Kalaparthi V,Seluanov A,Gorbunova V,Sokolov I

更新日期：2016-07-12 00:00:00

abstract：:Precise information regarding the transmembrane topology of mitochondrial porin is essential for understanding the mechanisms by which this protein functions. Porin acts as a channel in the outer membrane and interacts with small solutes and proteins to regulate mitochondrial function. The acquisition of high-resoluti...

journal_title：Biophysical journal

authors： Bay DC,O'Neil JD,Court DA

更新日期：2008-01-15 00:00:00

abstract：:Myofilament extensibility is a key structural parameter for interpreting myosin cross-bridge kinetics in striated muscle. Previous studies reported much higher thick-filament extensibility at low tension than the better-known and commonly used values at high tension, but in interpreting mechanical studies of muscle, a...

journal_title：Biophysical journal

authors： Ma W,Gong H,Kiss B,Lee EJ,Granzier H,Irving T

更新日期：2018-10-16 00:00:00

abstract：:Fluorescence lifetimes of dimeric rabbit muscle creatine kinase specifically dansylated at both active sites and the homologous monomeric lobster muscle arginine kinase singly dansylated were determined using phase-modulation methods with global analysis of overdetermined data sets. For both proteins, the data is adeq...

journal_title：Biophysical journal

authors： Grossman SH

更新日期：1991-03-01 00:00:00

abstract：:Communication-deficient cells (the SKHep1 cell line) were stably transfected with a plasmid containing cDNA which encodes the major gap junction protein of rat liver, connexin32. Application of the dual whole-cell voltage clamp technique with patch electrodes to pairs of transfected SKHep1 cells revealed strong sensit...

journal_title：Biophysical journal

authors： Moreno AP,Eghbali B,Spray DC

更新日期：1991-11-01 00:00:00

abstract：:High-efficiency electrofusion between cells of different sizes was achieved by application of fusing electric pulses to cells in centrifuged pellets. Larger target cells (Chinese hamster ovary or L1210 cells) were stacked among smaller human erythrocytes or erythrocyte ghosts by sequential centrifugation at 700 g to f...

journal_title：Biophysical journal

authors： Li LH,Hensen ML,Zhao YL,Hui SW

更新日期：1996-07-01 00:00:00

abstract：:High-affinity and cooperative binding of two Ca(2+) per ATPase (SERCA) occurs within the membrane-bound region of the enzyme. Direct measurements of binding at various Ca(2+) concentrations demonstrate that site-directed mutations within this region interfere selectively with Ca(2+) occupancy of either one or both bin...

journal_title：Biophysical journal

authors： Inesi G,Zhang Z,Lewis D

更新日期：2002-11-01 00:00:00

abstract：:By using an analogy between the magnetization of a paramagnetic material in an external magnetic field and the electric polarization of the lateral wall of outer hair cells in response to the transmembrane potential, we show that, based on experimental data on the charge transfer across the membrane, it is impossible ...

journal_title：Biophysical journal

authors： Scherer MP,Gummer AW

更新日期：2005-05-01 00:00:00

abstract：:Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique. Spatially resolved fluorescence decays from single mitochondria or only a very few organelles o...

journal_title：Biophysical journal

authors： Ramadass R,Bereiter-Hahn J

更新日期：2008-10-01 00:00:00

abstract：:Some membrane peptides, such as Alamethicin, form barrel-stave aggregates with a broad probability distribution of size (number of peptides in the aggregate). This distribution has been shown to depend on the characteristics of the lipid bilayer. A mechanism for this influence is suggested, in analogy to earlier work ...

journal_title：Biophysical journal

authors： Cantor RS

更新日期：2002-05-01 00:00:00

abstract：:Kinetic measurements of the self-assembly of proteins into amyloid fibrils are often used to make inferences about molecular mechanisms. In particular, the lag time--the quiescent period before aggregates are detected--is often found to scale with the protein concentration as a power law, whose exponent has been used ...

journal_title：Biophysical journal

authors： Eden K,Morris R,Gillam J,MacPhee CE,Allen RJ

更新日期：2015-02-03 00:00:00

abstract：:The characteristic equatorial X-ray pattern from a relaxed vertebrate skeletal muscle changes when the muscle is activated. In particular, there is a simultaneous decrease in the intensity of the first reflection (I10) and increase in the intensity of the second (I11). This observed change is almost reciprocal. When c...

journal_title：Biophysical journal

authors： Lymn RW

更新日期：1978-01-01 00:00:00

abstract：:The (31)P-nuclear magnetic resonance chemical shift of phosphatidic acid in a membrane is sensitive to the lipid head group packing and can report qualitatively on membrane lateral compression near the aqueous interface. We have used high-resolution (31)P-nuclear magnetic resonance to evaluate the lateral compression ...

journal_title：Biophysical journal

authors： Traïkia M,Warschawski DE,Lambert O,Rigaud JL,Devaux PF

更新日期：2002-09-01 00:00:00

abstract：:Glucagon solutions at pH 2.0 were subjected to mechanical agitation at 37 degrees C in the presence of a hydrophobic surface to explore the details of aggregation and fiber formation. High-resolution intermittent-contact atomic force microscopy performed in solution revealed the presence of aggregates after 0.5 h; how...

journal_title：Biophysical journal

authors： De Jong KL,Incledon B,Yip CM,DeFelippis MR

更新日期：2006-09-01 00:00:00

abstract：:The vibrational energy relaxation of a selected vibrational mode in cytochrome c--a C-D stretch in the terminal methyl group of Met80--has been studied using equilibrium molecular dynamics simulation and normal mode analysis methods. As demonstrated in the pioneering work of Romesberg and co-workers, isotopic labeling...

journal_title：Biophysical journal

authors： Bu L,Straub JE

更新日期：2003-09-01 00:00:00

abstract：:The microsecond folding and unfolding kinetics of ovine prion proteins (ovPrP) were measured under various solution conditions. A fragment comprising residues 94-233 of the full-length ovPrP was studied for four variants with differing susceptibilities to classical scrapie in sheep. The observed biexponential unfoldin...

journal_title：Biophysical journal

authors： Chen KC,Xu M,Wedemeyer WJ,Roder H

更新日期：2011-09-07 00:00:00

abstract：:Glutamic acid [(L-glu)n] + dihydrogen phosphate systems are studied by infrared (IR) spectroscopy dried and hydrated at 75% relative humidity, as a function of both the phosphate-glutamic acid residue (Pi/glu) ratio and the type of cations present. It is shown that the glutamic acid groups form hydrogen-bonded chains ...

journal_title：Biophysical journal

authors： Burget U,Zundel G

更新日期：1987-12-01 00:00:00

abstract：:The interaction between two stiff parallel DNA molecules is discussed using linear Debye-Hückel screening theory with and without inclusion of the dielectric discontinuity at the DNA surface, taking into account the helical symmetry of DNA. The pair potential furthermore includes the amount and distribution of counter...

journal_title：Biophysical journal

authors： Harreis HM,Likos CN,Löwen H

更新日期：2003-06-01 00:00:00

abstract：:Mitochondria often reside in subcellular regions with high metabolic demands. We examined the mechanisms that can govern the relocation of mitochondria to these sites in respiratory neurons. Mitochondria were visualized using tetramethylrhodamineethylester, and their movements were analyzed by applying single-particle...

journal_title：Biophysical journal

authors： Mironov SL

更新日期：2007-04-15 00:00:00

abstract：:Oriented purple membranes were equilibrated under controlled (2)H(2)O relative humidity ranging from 15% to 93% and introduced into the magnetic field of an NMR spectrometer with the membrane normal parallel to the magnetic field direction. Deuterium solid-state NMR spectra of these samples resolved four deuteron popu...

journal_title：Biophysical journal

authors： Bechinger B,Weik M

更新日期：2003-07-01 00:00:00

abstract：:Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen-rich biological tissues. NOSE is based on polarization-dependent second harmonic generation imaging. NOSE was used to access the molecular-level distribution of collagen fibril orientation relative to the local fiber...

journal_title：Biophysical journal

authors： Dow XY,DeWalt EL,Sullivan SZ,Schmitt PD,Ulcickas JRW,Simpson GJ

更新日期：2016-10-04 00:00:00

abstract：:By combining a description of the coupled solute and water flows through the in vivo cornea with a set of appropriate mechanical equilibrium conditions, it is possible to calculate directly the corneal thickness, given the corneal temperature, the state of the aqueous and tears, the swelling pressure-hydration relatio...

journal_title：Biophysical journal

authors： Friedman MH

更新日期：1972-06-01 00:00:00

abstract：:The analysis of single-molecule fluorescence resonance energy transfer (FRET) trajectories has become one of significant biophysical interest. In deducing the transition rates between various states of a system for time-binned data, researchers have relied on simple, but often arbitrary methods of extracting rates fro...

journal_title：Biophysical journal

authors： McKinney SA,Joo C,Ha T

更新日期：2006-09-01 00:00:00

abstract：:Although ubiquitous, the processes by which bacteria colonize surfaces remain poorly understood. Here we report results for the influence of the wall shear stress on the early-stage adhesion of Pseudomonas aeruginosa PA14 on glass and polydimethylsiloxane surfaces. We use image analysis to measure the residence time o...

journal_title：Biophysical journal

authors： Lecuyer S,Rusconi R,Shen Y,Forsyth A,Vlamakis H,Kolter R,Stone HA

更新日期：2011-01-19 00:00:00

abstract：:A subsarcolemmal tubular system network (SSTN) has been detected in skeletal muscle fibers by confocal imaging after the removal of the sarcolemma. Here we confirm the existence and resolve the fine architecture and the localization of the SSTN at an unprecedented level of detail by examining extracellularly applied t...

journal_title：Biophysical journal

authors： Jayasinghe ID,Lo HP,Morgan GP,Baddeley D,Parton RG,Soeller C,Launikonis BS

更新日期：2013-06-04 00:00:00

abstract：:Each gating mechanism controlling permeability in a membrane may be influenced by only a few charge binding sites on the membrane surface, so that fluctuations in the occupancy of these sites are important. Tow extreme cases arise. (a) The time scale of these fluctuations is much shorter than the gating time constant....

journal_title：Biophysical journal

authors： Attwell D,Eisner D

更新日期：1978-12-01 00:00:00

abstract：:In vitro and in situ tests have been run to characterize the reaction of the mettalochromic indicator, arsenazo III, with calcium. Job plots as well as plots of indicator absorbance vs. [Ca2+] at different indicator concentrations show a 1:1 reaction stoichiometry. Equilibrium analysis and analysis using Adair's equat...

journal_title：Biophysical journal

authors： Ahmed Z,Kragie L,Connor JA

更新日期：1980-12-01 00:00:00

abstract：:The adhesion of red blood cells (RBCs) to cells or surfaces is of current basic science and clinical interest yet the details governing this process are still being explored. In this study, the effects of nonadsorbing polymers on the adhesion of RBC to albumin-coated glass have been investigated employing interference...

journal_title：Biophysical journal

authors： Zhang ZW,Neu B

更新日期：2009-08-19 00:00:00