Abstract：
OBJECTIVES:An increase in oxidative stress may contribute to the development of oxidative protein damage in the aging rat skeletal muscle. Our aim was to reveal protein carbonyl (PCO), advanced oxidation protein products (AOPP), a novel marker of oxidative stress, and protein thiol (P-SH) levels as markers of protein oxidation, as well as lipid hydroperoxide (LHP) levels as a marker of lipid peroxidation, and relation of nitrotyrosine (NT) levels with these markers in skeletal muscle tissue of young, adult, and old male Wistar rats. DESIGN AND METHODS:In the present study, we investigated the relation between aging and oxidative protein damage parameters such as PCO, NT, AOPP, and P-SH, as well as oxidative stress parameters such as total thiol, nonprotein thiol, and LHP in the skeletal muscle tissue of young, adult, and old Wistar rats. RESULTS:PCO and NT levels of old rats were significantly increased compared with those of young and adult rats. Skeletal muscle AOPP levels were significantly increased in old rats compared with those of adult rats. P-SH levels were significantly decreased in old rats compared with those of young rats. CONCLUSIONS:The finding that the increase in PCO levels of young vs. old group was more significant than that of adult vs. old group may suggest that PCO formation is an early specific marker of aging process in skeletal muscle. In addition, increased levels of nitrotyrosine in the skeletal muscle of the old rat group may be a novel specific marker of oxidative protein damage in the aging muscle. The absence of correlation between oxidative protein damage markers mentioned above and LHP levels may indicate that protein oxidation and lipid peroxidation in the aging rat skeletal tissue are two distinct mechanisms.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Cakatay U,Telci A,Kayali R,Tekeli F,Akçay T,Sivas Adoi
10.1016/s0009-9120(02)00407-1subject
Has Abstractpub_date
2003-02-01 00:00:00pages
51-5issue
1eissn
0009-9120issn
1873-2933pii
S0009912002004071journal_volume
36pub_type
杂志文章abstract：:Preparation of (14C) Pro labeled protocollagen and requirements for its hydroxylation by PPH was investigated. Protocollagen can be prepared by centrifugation of the biological material at 15.000 X g. Substances in current use, viz catalase, bovine serum albumin and dithiothreitol were found unnecessary under the hydr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(76)80072-0
更新日期：1976-12-01 00:00:00
abstract：OBJECTIVES:In this study we aimed to assess the changes in pro-oxidant-antioxidant balance (PAB) after the placement of either a drug-eluting-stent (DES) or bare-metal-stent (BMS) in patients with stable coronary artery disease. DESIGN AND METHODS:Percutaneous coronary interventions (PCI) with either BMS or DES were u...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.10.009
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVES:Mucopolysaccharidoses (MPSs) are a group of rare, inherited metabolic disorders which result from the lack of one of the lysosomal enzymes responsible for the degradation of glycosaminoglycans. Early recognition of MPS is important as it enables prompt implementation of enzyme replacement therapy (ERT). Dipe...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.11.011
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVES:The present study was designed to (a) evaluate and compare plasma ammonia levels (PAL) in patients with acute liver failure (ALF) and chronic liver disease (CLD) with or without hepatic encephalopathy (HE); (b) correlate the severity of HE with PAL; and (c) correlate PAL with clinical features of raised intr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.04.013
更新日期：2005-08-01 00:00:00
abstract：:An automated biotin-streptavidin procedure for measuring progesterone in serum is described. The method is linear up to 98.3 nmol/L and the calibration curve is stable for at least 14 days. The lower limit of progesterone detection is 0.70 nmol/L. Although serum is the preferred specimen, progesterone levels can be me...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(92)90105-2
更新日期：1992-12-01 00:00:00
abstract：:We present data comparing the Roche CEA radioimmunoassay (RIA) utilizing ultrafiltration and the Abbott CEA immunoradiometric assay (IRMA) methods for standard curve sensitivity, analytical reproducibility, recovery (pre and post extraction), dilution linearity, and patient correlation. The Roche intra- and inter-assa...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(82)97007-2
更新日期：1982-10-01 00:00:00
abstract：:1. Six subjects were investigated during the luteal phase of the menstrual cycle to determine whether the ovarian steroids, estrogen and progesterone were released in an episodic manner. 2. Analysis of plasma obtained every 20 min. during a normal working day provided no evidence for pulsatile release of either steroi...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(75)92479-0
更新日期：1975-08-01 00:00:00
abstract：:Thyroxine binding protein characteristics were defined in 6 normal subjects, 4 with thyroxine binding globulin (TBG) deficiency, 3 with TBG increase, one with hyperthyroxinemic dysalbuminemia, 7 with severe non-thyroidal illness, and 3 with chronic renal failure. Free thyroxine was measured by Sephadex partition in pl...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(86)80073-x
更新日期：1986-02-01 00:00:00
abstract：PURPOSE:Dermatan sulfate (DS) plays a number of roles in a wide range of biological activities such as cell signaling and tissue morphogenesis through interactions with various extracellular matrix proteins including collagen. Mutations in the carbohydrate sulfotransferase 14 gene (CHST14) encoding CHST14/dermatan 4-O-...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.02.018
更新日期：2017-08-01 00:00:00
abstract：OBJECTIVES:This study was undertaken to evaluate the relationship between serum bilirubin concentrations and the degree of urinary albumin excretion in hypertensive patients. DESIGN AND METHODS:A total of 120 hypertensive subjects were enrolled, in which 80 (67%) with normoalbuminuria (albumin excretion rate [AER] of ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.03.137
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVES:This study investigates how some of human physiological parameters, in particular sex and daytime, can contribute to the normal variation in blood gene expression. DESIGN AND METHODS:The expressions of two genes: SOD1 (Cu/Zn) and SOD2 (Mn) in white blood cells of 19 healthy individuals (10 men and 9 women) ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2008.08.064
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVES:To establish whether normal human subjects excrete glycine betaine at a constant rate. DESIGN AND METHODS:Urine was collected from ten normal healthy male subjects for 14 days, during which fluid intake was systematically varied from <800 mL to >3 L per day. Glycine betaine, sorbitol and creatinine excretio...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2006.10.029
更新日期：2007-04-01 00:00:00
abstract：OBJECTIVES:To explore the impact of response to interferon and ribavirin antiviral therapy on human growth hormone (hGH) levels in Egyptian chronic hepatitis C genotype-4 infected patients. DESIGN AND METHODS:We studied eighty Egyptian HCV infected patients visiting outpatient clinics of Tropical Medicine and Hepatolo...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.08.1145
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVES:Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. DESIGN AND METHODS:The method is based ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.05.019
更新日期：2011-08-01 00:00:00
abstract：:We report a case of metastatic adenocarcinoma of the prostate with widespread bony and hepatic involvement having extremely high BB isoenzyme of creatine kinase in serum (2405 U/liter). The serum LD5/LD1 ratio is greater than one, with total LD activity more than twenty-five fold the normal value. The homogenate of pr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(80)90502-0
更新日期：1980-02-01 00:00:00
abstract：OBJECTIVE:To review biochemical literature and determine the significance of non-covalent bonding in plasma. RESULTS:Many non-covalent bonds exist in plasma. Relatively strong self-associations are found for serum albumin, several gamma-globulins, apolipoproteins and fibrin(ogen). Here, fibrinogen and fibrin, consider...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(96)00159-2
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVES:To develop immunofluorometric procedures for measuring BRCA1 protein in various biological fluids and tissue extracts. DESIGN AND METHODS:Five commercially available monoclonal and polyclonal antibodies against BRCA1 were evaluated for developing competitive and non-competitive immunofluorometric procedures...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(97)00042-8
更新日期：1997-07-01 00:00:00
abstract：OBJECTIVES:This study aimed to examine the association between promoter polymorphisms of Th1 and Th2 cytokine genes [interleukin-4 (IL-4 T-34C, A-81G, C-285T and T-589C), IL-6 (G-174C), IL-10 (A-592C and T-819C) and tumour necrosis factor-alpha (TNF-alpha G-238A and G-308A)] and Graves' disease (GD) in Taiwanese popula...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2006.11.009
更新日期：2007-02-01 00:00:00
abstract：OBJECTIVE:To identify independent predictors of serum butyrylcholinesterase (BuChE) activity and examine its relationship with the metabolic syndrome. DESIGN AND METHODS:Serum BuChE activity was measured and metabolic syndrome risk factors were assessed in 1097 healthy subjects recruited from the Newfoundland adult po...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.04.008
更新日期：2005-09-01 00:00:00
abstract：:We describe a simple and reliable radioimmunoassay (RIA) method for the measurement of human placental lactogen (hPL) in undiluted serum. Standard curves, in serum, cover the range of 0.015 to 1 mlU/L for early pregnancy monitoring (7 to 15 weeks, referred to as Procedure I), and 0.531 to 17 mIU/L for mid and late pre...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(82)90753-6
更新日期：1982-04-01 00:00:00
abstract：:The toxicity of carbon monoxide has been recognized for long throughout history and is unquestionably the leading cause of unintentional poisoning deaths in the Western countries. The severity of poisoning is dependent upon environmental and human factor. The leading pathophysiological mechanism resides in the ability...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2012.06.004
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVES:To develop an accurate assay and establish the normal reference intervals for serum cortisol, corticosterone, 11-deoxycortisol, androstenedione, 21-hydroxyprogesterone, testosterone, 17-hydroxyprogesterone, and progesterone. These steroids are commonly used as biomarkers for the diagnosis and monitoring of e...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.01.002
更新日期：2013-05-01 00:00:00
abstract：BACKGROUND:The p53 gene is an established tumor suppressor and an inducer of apoptosis. We here attempt to determine whether the putative anticarcinogenic properties attributed to red wine and its polyphenolic constituents depend, at least in part, upon their ability to modulate p53 expression in cancer cells. METHODS...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(01)00246-6
更新日期：2001-07-01 00:00:00
abstract：OBJECTIVES:The new immunochemistry cobas e 801 module (Roche Diagnostics) was developed to meet increasing demands on routine laboratories to further improve testing efficiency, while maintaining high quality and reliable data. DESIGN AND METHODS:During a non-interventional multicenter evaluation study, the overall pe...
journal_title：Clinical biochemistry
pub_type： 杂志文章,多中心研究
doi：10.1016/j.clinbiochem.2018.05.019
更新日期：2018-08-01 00:00:00
abstract：BACKGROUND:High levels of intact and cleaved forms of the urokinase-type plasminogen activator receptor (uPAR) in both tissue and blood are associated with poor survival in several cancer diseases. The prognostic significance of uPAR in cholangiocarcinoma is unknown. The aims of this study were to determine if pre-trea...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.01.030
更新日期：2014-05-01 00:00:00
abstract：OBJECTIVES:We evaluated the effects of lifestyle modification (LM) on lipid profile, oxidative stress and serum-stimulated human coronary artery endothelial cell (HCAEC) viability in coronary artery disease (CAD) patients after 6months. DESIGN AND METHODS:Thirty patients with CAD were randomly assigned to LM intervent...
journal_title：Clinical biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.clinbiochem.2010.04.056
更新日期：2010-07-01 00:00:00
abstract：UNLABELLED:Antioxidant capacity testing is commonly used in clinical investigations to provide an estimate of in vitro antioxidant capacity of biosamples. Although beneficial to measure the synergistic contribution of all compounds with antioxidant functionality, assessing the capacity of non-protein fractions or small...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.06.021
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:To develop sensitive nonisotopic assays for protein-tyrosine phosphatase (PTP) activity. METHODS:The fluorometric assay is based on the fact that phosphotyrosine but not tyrosine forms highly fluorescent complexes with Tb3+. Thus, PTP activity can be followed by measuring the decrease of fluorescence due to ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(95)02026-8
更新日期：1996-04-01 00:00:00
abstract：OBJECTIVE:Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft. DESIGN AND METHODS:Hair sample was pulverized and incuba...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.01.014
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVES:The International Federation of Clinical Chemistry (IFCC) initiated a pilot study, with cortisol as an example, that aims to implement the concept of standardization of hapten immunoprocedures on the basis of metrological traceability. In fact, this standardization concept comes down to correct calibration o...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(98)00037-x
更新日期：1998-08-01 00:00:00
