Abstract:
:The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for virus growth in non-permissive cells such as H9. To elucidate the mechanism of action of the Vif protein, vif mutants, which show trans-dominant negative effects on the replication of HIV-1, would be useful tools. In this study, a new assay system to identify the mutants of this category was established. For this new system, various reporter clones carrying both mutant and authentic vif sequences were generated. By determining the growth ability of the viruses derived from the reporter constructs, the potential negative effect of the mutant vif sequence was readily and sensitively monitored. Ten vif mutant sequences tested were found not to exert the trans-dominant negative effect on the replication of HIV-1.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Fujita M,Matsumoto S,Sakurai A,Doi N,Miyaura M,Yoshida A,Adachi Adoi
10.1016/s1286-4579(02)01646-5subject
Has Abstractpub_date
2002-10-01 00:00:00pages
1203-7issue
12eissn
1286-4579issn
1769-714Xpii
S1286457902016465journal_volume
4pub_type
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