High glutamate decreases S100B secretion stimulated by serum deprivation in astrocytes.

Abstract:

:S100B is a calcium-binding protein expressed and secreted by astrocytes, playing a neurotrophic role in neighboring cells. A protective role of the S100B against glutamate-induced excitotoxicity has recently been proposed. We investigated S100B secretion in rat hippocampal astrocytes exposed to high concentrations of glutamate during serum deprivation (stimulated condition) or not (basal condition), for 30 min. Glutamate at 1 mM had no effect on basal secretion of S100B, but it decreased S100B secretion in serum-deprived astrocytes after 1 h. Secretion was inhibited by Rp-cAMPS or H89. In addition, serum deprivation was accompanied by a transitory increase of intracellular content of cAMP. Our results suggest that high levels of glutamate in a serum-deprived condition could impair S100B secretion from hippocampal astrocytes.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Gonçalves D,Karl J,Leite M,Rotta L,Salbego C,Rocha E,Wofchuk S,Gonçalves CA

doi

10.1097/00001756-200208270-00009

subject

Has Abstract

pub_date

2002-08-27 00:00:00

pages

1533-5

issue

12

eissn

0959-4965

issn

1473-558X

journal_volume

13

pub_type

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