Abstract:
:Green fluorescent protein (GFP) gene was transfected and expressed in murine embryonic stem (ES) cells under the control of the astrocyte-specific glial fibrillary acidic protein (GFAP) promoter. Stably transfected cells were characterized by immunohistochemistry and by fluorescence microscopy. Cells containing GFP were differentiated to Type I and Type II astrocytes after induction by all-trans retinoic acid. Differentiated cells were expressed GFP and visualized by fluorescence microscopy. Differentiated cells expressed GFP were correlated with the expression of GFAP and morphological change. It demonstrates that the cell line expressed GFP can be used to trace the morphological changes of astrocytes during differentiation, and further for the isolation of astrocytes from the mixed cells differentiated from ES cell.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Tang F,Shang K,Wang X,Gu Jdoi
10.1023/a:1015397829913subject
Has Abstractpub_date
2002-02-01 00:00:00pages
95-101issue
1eissn
0272-4340issn
1573-6830journal_volume
22pub_type
杂志文章abstract::Symptoms like mental retardation, depression, and anxiety have been observed during aging. Almost similar phenotypes have been evident in patients having haploinsufficiency or mutations in Pax6, a transcriptional regulator. Since Pax6 regulates axon guidance, differentiation of neurons from glia, and neuronal migratio...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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更新日期:2017-04-01 00:00:00
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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