Abstract：
:Bacterial surface layers (S-layers) are extracellular protein networks that act as molecular sieves and protect a large variety of archaea and bacteria from hostile environments. Atomic force microscopy (AFM) was used to asses the S-layer of Coryne-bacterium glutamicum formed of PS2 proteins that assemble into hexameric complexes within a hexagonal lattice. Native and trypsin-treated S-layers were studied. Using the AFM stylus as a nanodissector, native arrays that adsorbed to mica as double layers were separated. All surfaces of native and protease-digested S-layers were imaged at better than 1 nm lateral resolution. Difference maps of the topographies of native and proteolysed samples revealed the location of the cleaved C-terminal fragment and the sidedness of the S-layer. Because the corrugation depths determined from images of both sides span the total thickness of the S-layer, a three-dimensional reconstruction of the S-layer could be calculated. Lattice defects visualized at 1 nm resolution revealed the molecular boundaries of PS2 proteins. The combination of AFM imaging and single molecule force spectroscopy allowed the mechanical properties of the Corynebacterium glutamicum S-layer to be examined. The results provide a basis for understanding the amazing stability of this protective bacterial surface coat.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Scheuring S,Stahlberg H,Chami M,Houssin C,Rigaud JL,Engel Adoi
10.1046/j.1365-2958.2002.02864.xsubject
Has Abstractpub_date
2002-05-01 00:00:00pages
675-84issue
3eissn
0950-382Xissn
1365-2958pii
2864journal_volume
44pub_type
杂志文章abstract：:The spoIIIG gene encodes a sigma factor that determines prespore-specific gene expression during sporulation in Bacillus subtilis. Correct spatial and temporal expression of the spoIIIG gene depends on a number of other sporulation (spo) genes, but only one of these genes, spoIIIE, has a specific effect on spoIIIG exp...
journal_title：Molecular microbiology
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doi：10.1111/j.1365-2958.1989.tb00275.x
更新日期：1989-09-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 评论,杂志文章
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更新日期：2015-07-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12666
更新日期：2014-07-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1990.tb00652.x
更新日期：1990-05-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：1994-03-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2008.06298.x
更新日期：2008-08-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1996.tb02619.x
更新日期：1996-04-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01969.x
更新日期：2000-07-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2001.02430.x
更新日期：2001-05-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.14567
更新日期：2020-10-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2007.06103.x
更新日期：2008-03-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.mmi_17040769.x
更新日期：1995-08-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2002.03133.x
更新日期：2002-10-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1996.386918.x
更新日期：1996-02-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb01272.x
更新日期：1994-10-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1996.00111.x
更新日期：1996-10-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb02671.x
更新日期：1993-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1365-2958.1990.tb00712.x
更新日期：1990-08-01 00:00:00
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更新日期：1999-02-01 00:00:00
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更新日期：2002-05-01 00:00:00
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更新日期：1996-05-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：1999-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2006-09-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2011-07-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2001-10-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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