Quantification of TECTA and DFNA5 expression in the developing mouse cochlea.

Abstract:

:TECTA and DFNA5 are the mouse orthologues of the human deafness-associated genes TECTA and DFNA5. To determine how expression of these genes is regulated during development, relative mRNA abundance was examined in mice by non-radioactive RT-PCR. TECTA mRNA was detected on embryonic day 15 (E15), increased to its highest level on postnatal day 3 (P3) and then dramatically decreased by P15. Low levels persisted (adulthood, P45 to 67) with mean mRNA abundance after P15 less than 25% of P3 levels. DFNA5 mRNA expression was constant throughout these time points. These results imply that TECTA is transcribed at a particularly high level during tectorial membrane morphogenesis. In contrast, DFNA5 is present in both the developing and mature cochlea.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Maeda Y,Fukushima K,Kasai N,Maeta M,Nishizaki K

doi

10.1097/00001756-200110290-00016

subject

Has Abstract

pub_date

2001-10-29 00:00:00

pages

3223-6

issue

15

eissn

0959-4965

issn

1473-558X

journal_volume

12

pub_type

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