Abstract：
:Urocortin is a newly identified member of the CRF neuropeptide family. Urocortin has been found to bind with high affinity to CRF receptors. The present study investigated urocortin and CRF receptor expression in human colonic mucosa. Non-pathologic sections of adult colorectal tissues were obtained from patients with colorectal cancer at surgery. Urocortin expression was examined using immunohistochemistry and messenger (m) RNA in situ hybridization. Isolated lamina propria mononuclear cells (LPMC) and epithelial cells were also analyzed by flow cytometry for the characterization of urocortin-positive cells, and by RT-PCR for detection of urocortin, CRF, and CRF receptor mRNA. Urocortin peptide distribution at various stages of human development (n = 35, from 11 weeks of gestation to 6 years of age) was examined by immunohistochemistry using surgical and autopsy specimens. Immunoreactive urocortin and urocortin mRNA were predominantly detected in lamina propria macrophages. Urocortin peptide expression was detected from as early as three months of age, but not before birth or in neonates. Urocortin, CRF receptor type 1 and type 2 alpha mRNA were detected in LPMC. CRF receptor type 2 beta mRNA, a minor isoform in human tissues, was also detected in LPMC, but at lower levels. Urocortin is locally synthesized in lamina propria macrophages and may act on lamina propria inflammatory cells as an autocrine/paracrine regulator of the mucosal immune system. The appearance of urocortin after birth indicates that the exposure to dietary intake and/or luminal bacteria after birth may contribute to the initiation of urocortin expression in human gastrointestinal tract mucosa.
journal_name
Peptidesjournal_title
Peptidesauthors
Muramatsu Y,Fukushima K,Iino K,Totsune K,Takahashi K,Suzuki T,Hirasawa G,Takeyama J,Ito M,Nose M,Tashiro A,Hongo M,Oki Y,Nagura H,Sasano Hdoi
10.1016/s0196-9781(00)00335-1subject
Has Abstractpub_date
2000-12-01 00:00:00pages
1799-809issue
12eissn
0196-9781issn
1873-5169pii
S0196978100003351journal_volume
21pub_type
杂志文章相关文献
PEPTIDES文献大全abstract：:alpha-MSH immunoreactive peptides were fractionated and characterized in rat and human brain and rat pituitary by reversed phase high pressure liquid chromatographic techniques. alpha-MSH and deacetylated alpha-MSH were two major naturally existing peptides in both brain and pituitary gland. Subsequent experiments exa...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(81)80126-x
更新日期：1981-10-01 00:00:00
abstract：:Opiate binding sites in five brain regions were labeled with the mu and delta markers, 3H-morphine and 3H-[D-Ala2,D-leu5]enkephalin, respectively. The highest densities of both 3H-morphine and 3H-DADLE labeled sites are found in striatum and frontal cortex. Hypothalamus and midbrain contain predominantly 3H-morphine l...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(83)90080-3
更新日期：1983-11-01 00:00:00
abstract：:Central nervous systems of the pond snail Lymnaea stagnalis were incubated in vitro in different concentrations of ORG 2766 (10(-9)-2.5 x 10(-4) M) for 10 and 20 h. Quantitative ultrastructural study of cross sections of the cerebral commissure showed that the number of microtubules in large axons had increased after ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(92)90185-6
更新日期：1992-07-01 00:00:00
abstract：:The functional help provided by four cross-linked synthetic peptides from HIV-1 Gag structural proteins was investigated in the mouse model. These peptides, selected upon non-self-criteria, are not predicted as T epitopes by classical prediction methods such as the Rothbard consensus or the amphipathy rule. Priming mi...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(94)90055-8
更新日期：1994-01-01 00:00:00
abstract：:A simple and computationally nonintensive technique based on principal component analysis of 3-dimensional fields to derive theoretical descriptors is presented. The descriptors are then applied to a quantitative structure-activity relationship study on bradykinin potentiating pentapeptides. ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(91)90198-x
更新日期：1991-11-01 00:00:00
abstract：:Glucocorticoids have been shown to decrease CRF receptor binding in the anterior pituitary. To determine whether glucocorticoids or CRF peptide modulate CRF-R1 gene expression, CRF-R1 mRNA levels in rat pituitary and brain were measured after administration of a synthetic glucocorticoid, dexamethasone (DEX) or rat/hum...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(96)00014-9
更新日期：1996-01-01 00:00:00
abstract：:The emergence of new and resistant viruses is a serious global burden. Conventional antiviral therapy with small molecules has led to the development of resistant mutants. In the case of hand, foot and mouth disease (HFMD), the absence of a US-FDA approved vaccine calls for urgent need to develop an antiviral that cou...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/j.peptides.2020.170443
更新日期：2020-11-07 00:00:00
abstract：:VIP stimulates adenylate cyclase activity of male and female rat anterior pituitaries and human prolactinomas, while dopamine inhibits the enzyme activity of female rat pituitaries and prolactinomas. A dopamine inhibited cyclase can be detected also in male rats provided the enzyme activity is increased by VIP. The an...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(84)90172-4
更新日期：1984-11-01 00:00:00
abstract：:125I-Ang I binding to atypical sites on Mycoplasma hyorhinis-contaminated IEC-18 cell membranes increased with increasing pH and [NaCl] (ED50 at 500 mM; maximal 13-fold increase at 2 M NaCl). Alkali metal chlorides and sodium halides increased binding with rank orders of Na+ < K+ < Rb+ < Cs+ = Li+ and F- < Cl- < Br < ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(98)00163-6
更新日期：1999-01-01 00:00:00
abstract：:Malaria lactate dehydrogenase, a glycolytic enzyme, is a malaria diagnostic target in lateral flow immunochromatographic rapid diagnostic tests. Recombinant Plasmodium yoelii LDH was cloned into the pET-28a vector, expressed and the expressed protein purified from a Ni-NTA affinity matrix. A pan-malarial LDH antibody ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2010.01.002
更新日期：2010-04-01 00:00:00
abstract：:High levels of neuropeptide Y (NPY) are found in the hypothalamus, median eminence, pituitary portal blood, and the pituitary in a number of species. Neuropeptide Y may influence the synthesis and secretion of a variety of hormones by interacting with specific receptors in the hypothalamus and/or the pituitary. To fur...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(94)90090-6
更新日期：1994-01-01 00:00:00
abstract：:Opioid receptor analyses of deltorphin A (H-Tyr-D-Met-Phe-His-Leu-Met-Asp-NH2) analogues indicated the following: (a) increased negativity differentially affected affinities (Ki) and selectivity (Ki mu/Ki delta); (b) shifted sequence heptapeptides, [Asp5,Leu6,Met-NH2(7)] and [Asp4,His5,Leu6,Met-NH2(7)], reversed selec...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(93)90006-3
更新日期：1993-01-01 00:00:00
abstract：:Several studies have documented cardiovascular effects of apelin, including enhanced inotropy and vasodilation. However, these cardiovascular effects are short lived due to the predicted short circulating half-life of the apelin peptide. To address this limitation of apelin, we pursued N-terminal PEGylation of apelin ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.09.003
更新日期：2012-11-01 00:00:00
abstract：:A 20-kDa protein with substantial N-terminal sequence homology to thaumatin-like proteins was isolated from ripe fruits of the emperor banana, Musa basjoo cv. 'emperor banana'. The isolation procedure entailed (NH(4))(2)SO(4) precipitation, ion exchange chromatography on DEAE-cellulose, and affinity chromatography on ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2007.01.005
更新日期：2007-04-01 00:00:00
abstract：:There is a well-documented association between cyclic changes to food intake and the changing ovarian hormone levels of the reproductive cycle in female mammals. Limited research on appetite-controlling gastrointestinal peptides has taken place in females, simply because regular reproductive changes in steroid hormone...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2017.02.010
更新日期：2017-04-01 00:00:00
abstract：:Angiotensin II binding sites were localized and quantified in individual brain nuclei from single rats by incubation of tissue sections with 1 nM 125I-[Sar1]-angiotensin II, [3H]-Ultrofilm autoradiography, computerized microdensitometry and comparison with 125I-standards. High angiotensin II binding was present in the...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(86)90044-6
更新日期：1986-07-01 00:00:00
abstract：:The prothoracicotropic hormones (PTTH) are cerebral peptides that control insect postembryonic development by stimulating the prothoracic glands to synthesize ecdysteroids. Using immunoaffinity chromatography and SDS-PAGE, a 25.5 kDa big PTTH has been purified from Manduca sexta. Based upon SDS-PAGE and Western blot a...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(93)90142-4
更新日期：1993-05-01 00:00:00
abstract：:Tachykinins are multifunctional neuropeptides that have been identified in vertebrates as well as invertebrates. The C-terminal FXGXRa-motif constitutes the consensus active core region of invertebrate tachykinins. In Drosophila, two putative G protein-coupled tachykinin receptors have been cloned: DTKR and NKD. This ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2006.06.014
更新日期：2007-01-01 00:00:00
abstract：:Recently, a new serine protease inhibitor family has been described in arthropods. Eight members were purified from locusts and 13 peptides have been identified by cDNA cloning. The peptides share a conserved cysteine array (Cys-Xaa(9-12)-Cys-Asn-Xaa-Cys-Xaa-Cys-Xaa(2-3)-Gly-Xaa(3-6)-Cys-Thr-Xaa(3)-Cys) with nine inhi...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2004.03.005
更新日期：2004-06-01 00:00:00
abstract：:In recent years our knowledge of the nervous control of the cerebral circulation has increased. The use of denervations and retrograde tracing in combination with immunohistochemical techniques has demonstrated that cerebral vessels are supplied with sympathetic, parasympathetic, and sensory nerve fibers and possibly ...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/s0196-9781(01)00408-9
更新日期：2001-06-01 00:00:00
abstract：:In the crayfish Astacus leptodactylus, as in several crustacean species, the crustacean hyperglycemic hormone is present as two isoforms differing by the chirality of the third residue, a phenylalanine. In the present work, isoforms synthesized full length by solid-phase peptide synthesis have been purified, refolded,...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.01.019
更新日期：2012-03-01 00:00:00
abstract：:Most small peptide hormones and neurotransmitters are highly flexible, conformationally labile molecules in aqueous and other environments. Thus efforts to determine the relationships between conformational properties of these peptides in aqueous and other solvents and their biological activities at membrane receptors...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(82)90095-x
更新日期：1982-05-01 00:00:00
abstract：:The teleost salmon calcitonin (sCT), but not mammalian CT, shows similar biologic actions in the skeletal muscle as amylin and calcitonin gene-related peptide (CGRP). The peptides have also been shown to reduce food intake in rams. Because sCT, but not amylin, binds irreversibly to amylin binding sites, the aim of the...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(99)00208-9
更新日期：2000-02-01 00:00:00
abstract：:There have been few reports on the effects of the brain-gut peptide motilin on the central nervous system (CNS). We administered motilin intracerebroventricularly to mice and investigated the effect of motilin on anxiety using an elevated plus-maze. Motilin produced a significant decrease in anxiety with an inverted U...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(98)00131-4
更新日期：1998-01-01 00:00:00
abstract：:The present study demonstrates the occurrence of substance P (SP)- and calcitonin gene related peptide (CGRP)-immunoreactive nerve fibres in bone, bone marrow, periosteum, synovial membrane and soft tissues adjacent to the bone. The distribution pattern of the two types of nerves was similar, although the CGRP-positiv...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(88)90023-x
更新日期：1988-01-01 00:00:00
abstract：:This study presents the binding and functional properties of the mouse NPFF(2) (mNPFF(2)) receptor, in comparison with its human counterpart (hNPFF(2)). Binding experiments were performed by using the NPFF(2) selective radioligand [(3)H]-EYF in membranes from CHO cells transfected with mouse and human NPFF(2) receptor...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2009.11.020
更新日期：2010-02-01 00:00:00
abstract：:In this work, we characterized the active site in the alpha-melanotropin hormone (alpha-MSH) sequence responsible for the enhancement of cAMP production in incubated striatal slices by using different alpha-MSH fragments. We also analyzed the effects of the co-incubation of the SCH23390, a dopaminergic D(1) antagonist...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(00)00211-4
更新日期：2000-06-01 00:00:00
abstract：:Several cardioactive peptides from the pulmonate snail Helix aspersa were tested for their effects on myocardial cAMP levels, but only the family of small cardioactive peptides (SCPs) were clearly effective. SCP increased cAMP in a dose dependent manner; the time course was phasic. The structure-activity relations of ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(96)00335-x
更新日期：1997-01-01 00:00:00
abstract：:The presence of the proenkephalin A-derived peptide Met5-enkephalin-Arg6-Gly7-Leu8 was demonstrated throughout the human gastrointestinal tract. Highest concentrations of Met5-enkephalin-Arg6-Gly7-Leu8, as assessed by radioimmunoassay, were measured in the separated muscularis externa, while lower levels were found in...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(86)90087-2
更新日期：1986-09-01 00:00:00
abstract：:Neuronostatin is a 13-amino acid amidated peptide widely distributed in various organs including gastrointestinal tract. However, the effect of neuronostatin on gastrointestinal motility has not been well characterized. In the present work, effects of central administration of neuronostatin on gastric emptying and gas...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.03.013
更新日期：2012-05-01 00:00:00
