Abstract:
BACKGROUND:Molecular analysis of plasma DNA during human pregnancy has led to the discovery that maternal plasma contains both fetal and maternal DNA. This valuable source of fetal DNA opens up new possibilities for noninvasive prenatal diagnosis. APPROACH:Published data from the last 3 years demonstrating the feasibility and utility of analyzing fetal DNA in maternal plasma are reviewed. CONTENT:The detection of fetal DNA in maternal plasma is much simpler and more robust than detecting fetal nucleated cells in maternal blood, and does not require prior enrichment. This approach has been shown to have application in the prenatal diagnosis of fetal rhesus D status, sex-linked disorders, and other paternally inherited genetic disorders. Abnormal fetal DNA concentrations in maternal plasma and serum have been found in common pregnancy-associated disorders, including preterm labor and preeclampsia, as well as in pregnancies complicated by fetal trisomy 21. After delivery, fetal DNA is cleared rapidly from maternal plasma, with a half-life in the order of minutes. These clearance kinetics exhibit an important difference from fetal cell clearance, where long-term persistence has been demonstrated. SUMMARY:It has been only 3 years since fetal DNA was first detected in maternal plasma, and much remains to be learned about the biology of this phenomenon. In addition, additional diagnostic applications beyond those discussed here can be expected in the near future.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Lo YMsubject
Has Abstractpub_date
2000-12-01 00:00:00pages
1903-6issue
12eissn
0009-9147issn
1530-8561journal_volume
46pub_type
杂志文章,评审abstract::Published estimates of the imprecision characteristics of immunoassays are often based on quantities of data that seem to be inadequate. The increasing use of imprecision profiles has not necessarily improved the situation. We describe and illustrate a method of computing 95% confidence intervals for profiles estimate...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND:Recent reports have described inherent problems with androgen immunoassays compared with mass spectrometry analyses. METHODS:We developed a method for measuring serum testosterone (T) and 5alpha-dihydrotestosterone (DHT) simultaneously via liquid-liquid extraction followed by liquid chromatography-tandem ma...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2008.103846
更新日期:2008-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:An association has been described between mortality in children with meningococcal disease and functional polymorphisms in the interleukin-1 (IL1) cluster. We undertook a multicenter study to evaluate associations of these polymorphisms in a Central European population. PATIENTS AND METHODS:The study involv...
journal_title:Clinical chemistry
pub_type: 杂志文章,多中心研究
doi:10.1373/clinchem.2005.058537
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:Cross-sectional studies have reported seasonal variation in high-sensitivity C-reactive protein (hsCRP). However, longitudinal data are lacking. METHODS:We collected data on diet, physical activity, psychosocial factors, physiology, and anthropometric measurements from 534 healthy adults (mean age 48 years,...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2008.111245
更新日期:2009-02-01 00:00:00
abstract::The concentrations of 25 plasma proteins were measured in 22 patients with membranous nephropathy. For some large proteins, the plasma concentrations were increased; there were also large proteins with low plasma concentrations, but small or medium-sized proteins showed uniformly lower plasma concentration than the co...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract:BACKGROUND:Cleavage of conjugates is an important step in toxicological analysis, especially of urine samples. The aim of this study was to combine the advantages and to reduce the disadvantages of acid hydrolysis and conventional enzymatic hydrolysis procedures. METHODS:beta-Glucuronidase (GRD; EC 3.2.1.31) and aryls...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1986-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::A rapid and precise immunoassay for quantification of total homocysteine in blood samples is presented. The method avoids the use of radioisotopes and chromatographic separations and relies on enzymatic conversion of homocysteine to S-adenosyl-L-homocysteine, followed by quantification of S-adenosyl-L-homocysteine by ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.077859
更新日期:2007-04-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1093/clinchem/hvaa121
更新日期:2020-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:An accurate determination of the major HFE mutation (C282Y), which is associated with hereditary hemochromatosis, is important in diagnosis and risk assessment for this disease. We report a single-tube high-throughput PCR method for the detection of C282Y. METHODS:We combined three previously described prin...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Carbohydrate-deficient transferrin (CDT) may now be the most valuable biological marker for diagnosis of alcohol abuse. We compared the diagnostic performance of two new CDT tests, Axis %CDT turbidimetric immunoassay (TIA) and Axis %CDT HPLC, against Specialty Laboratories' isoelectric focusing/immunoblotting/laser de...
journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract:BACKGROUND:Detection of melanoma cells in circulation may be important in assessing tumor progression. The objective of this study was to develop a specific, reliable multimarker quantitative real-time reverse transcription-PCR (qRT) assay for detecting melanoma cells in patients' blood. METHODS:We developed qRT assay...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2004.045096
更新日期:2005-06-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1093/clinchem/hvaa207
更新日期:2020-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.3.389
更新日期:2003-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-12-01 00:00:00
abstract:BACKGROUND:It is important that cardiac troponin be measured accurately with a robust method to limit false results with potentially adverse clinical outcomes. In this study, we characterized the robustness of 4 analytical platforms by measuring the outlier rate between duplicate results. METHODS:We measured cardiac t...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2010.159830
更新日期:2011-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2012.186866
更新日期:2012-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00