Abstract:
PURPOSE:To compare paired human corneas after storage at 4 degrees C in Chen medium (CM) and Optisol-GS medium (OM) for 7, 10, 14, and 21 days. METHODS:One cornea of each pair from nine human donors was randomly stored in either CM or OM, with its mate cornea stored in the other medium. Three pairs of corneas were stored for 7 days and two pairs each were stored for 10, 14, and 21 days at 4 degrees C. Baseline corneal thickness measurements and endothelial photographs were obtained with a specular microscope. Corneal thickness measurements were also taken on days 7, 10, 14, and 21 of storage. At the end of storage, the corneas were warmed 2 hours before endothelial photographs were taken and were then placed in fixative. A corneal endothelial analysis system was used to compare changes in endothelial size and shape after storage. After fixation, the corneal endothelium was examined by scanning electron microscopy (SEM), and TdT-dUTP terminal nick-end labeling (TUNEL) assays with 4'6-diamidino-2-phenylindole (DAPI) counterstaining were performed on tissue sections of each cornea. A laser scanning confocal microscope and an automated digital analysis system were used to detect the presence of TUNEL-positive apoptotic cells in each cell layer and to determine keratocyte densities. RESULTS:Mean corneal thickness at 0, 7, 10, 14, 21 days of storage was 0.69 +/- 0.05 mm, 0.69 +/- 0.06 mm, 0.73 +/- 0.08 mm, 0.87 +/- 0.04 mm, and 0.87 +/- 0.03 mm, respectively, for CM and 0.65 +/- 0.06 mm, 0.59 +/- 0.07 mm, 0.63 +/- 0.03 mm, 0.60 +/- 0.03 mm, and 0.69 +/- 0.02 mm, respectively, for OM (p < 0.0001). The mean decrease in endothelial cell density at the end of the 7-, 10-, and 14-day storage periods was 11 +/- 10% for the CM corneas and 5 +/- 5% for the OM corneas (p = 0.18). SEM showed an intact endothelial monolayer in all corneas. The mean percentages of TUNEL-positive cells in epithelium, stroma, and endothelium of CM-stored corneas were 4 +/- 4%, 2 +/- 3%, and 0.1 +/- 0.3%, respectively, and did not differ from the OM-stored corneal values of 4 +/- 3%, 2 +/- 4%, and 0.9 +/- 1.5%. The percentage of TUNEL-positive cells did not increase with storage time. Keratocyte density was 368 +/- 130 cells/mm2 for CM-stored corneas and 447 +/- 96 cells/mm2 for OM-stored corneas (p = 0.13). CONCLUSIONS:Corneas stored in CM were thicker during storage than those stored in OM. The two storage media did not differ with respect to endothelial cell loss during storage or to the percentage of TUNEL-positive cells or keratocyte density at the end of the storage period.
journal_name
Corneajournal_title
Corneaauthors
Nelson LR,Hodge DO,Bourne WMdoi
10.1097/00003226-200011000-00005subject
Has Abstractpub_date
2000-11-01 00:00:00pages
782-7issue
6eissn
0277-3740issn
1536-4798journal_volume
19pub_type
杂志文章相关文献
CORNEA文献大全abstract::Keratoconus is a bilateral disorder of corneal shape which may be sporadic or genetically determined. Early corneal thinning suggests that a functional loss of structural elements is a primary event in the disease. Tensile strength of the cornea is reduced and is expressed by signs of rupture and scarring in Bowman's ...
journal_title:Cornea
pub_type: 杂志文章,评审
doi:
更新日期:1988-01-01 00:00:00
abstract::A serial study was carried out with the scanning electron microscope every 5 h to examine the endothelial alterations that occur when the rabbit cornea remains in the eye after enucleation. The findings are compared with those encountered when the cornea with a scleral rim was removed from the globe immediately after ...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract:PURPOSE:To evaluate and compare the in vitro antimicrobial activity of levofloxacin versus ciprofloxacin and ofloxacin against ocular isolates from patients with bacterial conjunctivitis. METHODS:The in vitro antimicrobial susceptibilities of ocular isolates to levofloxacin, ofloxacin, and ciprofloxacin were determine...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-200104000-00012
更新日期:2001-04-01 00:00:00
abstract::Utilizing transmission electron microscopy, the authors describe results obtained from cellulose acetate impressions of the bulbar conjunctiva in acquired immunodeficiency syndrome patients with cytomegalovirus (CMV) retinitis. Particles consistent with a retrovirus were isolated, but no CMV particles were demonstrate...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-199111000-00008
更新日期:1991-11-01 00:00:00
abstract:PURPOSE:To identify risk factors for ocular graft-versus-host disease (oGVHD) in children with graft-versus-host disease (GVHD). METHODS:This retrospective cohort study identified 38 children diagnosed with GVHD who underwent an ophthalmological examination. Survival to onset of oGVHD after transplant was analyzed usi...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002659
更新日期:2021-01-18 00:00:00
abstract:PURPOSE:To report intraoperative and 1-year postoperative results of the pachy-bubble technique for deep anterior lamellar keratoplasty (DALK). METHODS:This prospective interventional case series included 110 eyes of 107 patients with anterior corneal pathology who underwent DALK, including 78 with keratoconus. Outcom...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000413
更新日期:2015-06-01 00:00:00
abstract:PURPOSE:The aim was to assess the clinical outcomes of corneal graft infections caused by multi-drug resistant Pseudomonas aeruginosa (MDR-PA). METHODS:This is a retrospective case series of 38 patients with corneal graft infections caused by MDR-PA managed from June to December 2011. Clinical and demographic details,...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000011
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:To evaluate and compare 1-year postoperative visual acuity, refractive results, endothelial cell density (ECD), and complications in a consecutive group of patients who underwent deep lamellar endothelial keratoplasty (DLEK) with those who underwent penetrating keratoplasty (PK) performed by 1 surgeon. METHODS...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e31815b8304
更新日期:2008-02-01 00:00:00
abstract:PURPOSE:To investigate the association of systemic diseases with keratoconus in the general Korean population. METHODS:This is a nationwide population-based retrospective cohort study using the Korean National Health Insurance Service-National Sample Cohort database from 2002 to 2015, involving 1,108,369 individuals. ...
journal_title:Cornea
pub_type: 杂志文章,多中心研究
doi:10.1097/ICO.0000000000002206
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:To compare intracameral 20% sulfur hexafluoride (SF6) versus 100% air as tamponade for graft attachment in Descemet membrane endothelial keratoplasty (DMEK). METHODS:Using an electronic database search on MEDLINE and CENTRAL from inception to December 2017, we performed a literature review and meta-analysis in...
journal_title:Cornea
pub_type: 杂志文章,meta分析,评审
doi:10.1097/ICO.0000000000001581
更新日期:2018-06-01 00:00:00
abstract::Two containers ("Iowa" and "Virginia") commonly used for transporting donor corneas, and various packing methods were evaluated for constancy of temperature maintenance. The larger Iowa container was found to be superior for extended storage. Packing methods that allowed the vials (containing McCarey-Kaufman medium) t...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate glaucoma onset and progression after implantation of Boston Keratoprostheses (B-KPro) and the role of glaucoma surgery. METHODS:Records of patients with B-KPro implantation during 2004 to 2009 were reviewed. Parameters relevant to B-KPro surgery and glaucoma status were re...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000067
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the association between a Mediterranean dietary pattern (MeDi) and Vitamin D levels on dry eye syndrome (DES). METHODS:This was a cross-sectional study. Male patients seen in the Miami Veterans Affairs eye clinic with normal eyelid, corneal, and conjunctival anatomy were r...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000089
更新日期:2014-05-01 00:00:00
abstract:PURPOSE:Recent new reports of possible iatrogenic transmission of Creutzfeldt-Jakob disease (CJD) in Europe have prompted renewed scrutiny of current Eye Bank Association of America criteria for evaluation of potential corneal donors in this country. A prior study evaluated the risk of CJD occurring in U.S. corneal don...
journal_title:Cornea
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract:PURPOSE:In our report, we present a suspected case of donor-derived Acanthamoeba keratitis after deep anterior lamellar keratoplasty. To the authors' knowledge, there have been no confirmed cases of Acanthamoeba keratitis transmission through corneal transplantation. METHODS:Deep anterior lamellar keratoplasty was per...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002517
更新日期:2020-09-16 00:00:00
abstract:PURPOSE:To test the hypothesis that patients with keratoconus and pellucid who have glaucoma or are glaucoma suspects have lower corneal hysteresis (CH) and/or corneal resistance factor (CRF) measurements compared with controls. METHODS:A prospective study at a tertiary eye center of patients with keratoconus and pell...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181ca363c
更新日期:2010-09-01 00:00:00
abstract:PURPOSE:To review the clinical course, treatment, and visual outcomes of keratitis with endophthalmitis caused by the filamentary fungus Fusarium. METHOD:One hundred fifty-nine cases of Fusarium keratitis at Bascom Palmer Eye Institute between January 1, 1987 and August 21, 2000 were reviewed. Ten patients with cultur...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-200305000-00004
更新日期:2003-05-01 00:00:00
abstract:PURPOSE:To compare efficiency and safety of epithelium-off corneal cross-linking (CXL) and transepithelial cross-linking (TE-CXL) in pediatric patients with progressive keratoconus. METHODS:Uncorrected and corrected visual acuity, corneal topography and pachymetry (Pentacam; Oculus Pentacam), and in vivo confocal micr...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e31826cf32d
更新日期:2013-05-01 00:00:00
abstract:PURPOSE:To compare the survival rate 1 year after Descemet membrane endothelial keratoplasty (DMEK) in pseudophakic eyes versus combined phacoemulsification and DMEK (triple-DMEK). METHODS:Retrospective, interventional, consecutive case series. From all DMEKs performed from January 1, 2017, to June 30, 2018, we select...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002636
更新日期:2020-12-22 00:00:00
abstract::Bacterial viability in bacterial adherence to new soft contact lenses (SCLs) was studied by exposing polymacon and lidofilcon A lenses to Pseudomonas aeruginosa: L, live, untreated bacteria; H, bacteria heated for 1 h at 100 degrees C; and P, bacteria treated with 3% hydrogen peroxide for 1 h. Cultures of groups L, H,...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:To identify risk factors for the occurrence of bleeding complications after enucleation for cornea donation, in order to develop preventive activities to reduce the occurrence of bleeding complications and especially the development of ocular hematomas. METHODS:From all Dutch cornea donors deceased in the year...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181a186fc
更新日期:2009-10-01 00:00:00
abstract:PURPOSE:A web-like retrocorneal membrane (RCM) is an uncommon complication of chronic syphilitic interstitial keratitis. Extracellular matrix components have not yet been defined in this structure, although previous histologic examinations have suggested the presence of collagen. We examined the presence and distributi...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-200101000-00019
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:We standardized quality-controlled cord blood serum (CBS)-based eye drops and evaluated the efficacy of 1-month CBS treatment in the healing of diseased corneal epithelium in severe dry eye (DE) patients. METHODS:Seventeen graft-versus-host disease (GVHD) and 13 Sjogren syndrome patients with severe persistent...
journal_title:Cornea
pub_type: 临床试验,杂志文章
doi:10.1097/ICO.0b013e3182580762
更新日期:2013-04-01 00:00:00
abstract::Mesenchymal stem cells isolated from connective tissues are pluripotent and differentiate into phenotypes of connective tissue cell lineages (osteoblasts, chondrocytes, and adipocytes) in vitro and in vivo. They have been used to treat mouse models of connective tissue disease such as lumican-null (Lum) and mucopolysa...
journal_title:Cornea
pub_type: 杂志文章,评审
doi:10.1097/ICO.0000000000001010
更新日期:2016-11-01 00:00:00
abstract:PURPOSE:To report a case of Acanthamoeba endophthalmitis after an uneventful cataract surgery. METHODS:Description, management, and outcomes of a biopsy-proven case of Acanthamoeba endophthalmitis. RESULTS:Two days after a routine cataract surgery elsewhere, the patient presented with acute endophthalmitis diagnosed ...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002289
更新日期:2020-08-01 00:00:00
abstract::Here we report a new method for image analysis of the corneal stroma. To obtain the biometric characteristics of a given area, we perform three different treatments of the same image. These automated predictions closely match each parameter (length, surface area, etc.), as measured manually by a "blind investigator." ...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-199107000-00009
更新日期:1991-07-01 00:00:00
abstract:PURPOSE:To describe a case of conjunctival malignant melanoma associated with pseudomelanomatous alteration of the apposing tarsal conjunctiva. METHODS:A 93-year-old woman presented with an elevated, pigmented mass on her superior bulbar conjunctiva. The tumor was associated with increased pigmentation of the apposing...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/01.ico.0000226358.26678.98
更新日期:2006-10-01 00:00:00
abstract:PURPOSE:To evaluate the effect of a single rebubbling procedure after Descemet membrane endothelial keratoplasty (DMEK) on corneal backscatter and endothelial cell loss (ECL). METHODS:Medical records of 130 consecutive DMEK cases (118 patients) were reviewed and 3 groups were formed: group 1 (n = 32), consisting of DM...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001554
更新日期:2018-07-01 00:00:00
abstract:PURPOSE:Lissamine green (LG) is often used in addition to fluorescein to assess the severity of conjunctival damage in dry eye syndrome, which is graded manually. Our purpose was to describe an algorithm designed for image analysis of LG conjunctival staining. METHODS:Twenty pictures of patients suffering from dry eye...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002617
更新日期:2020-12-18 00:00:00
abstract:PURPOSE:To study changes in structure and barrier function of the corneal epithelium after penetrating keratoplasty. METHODS:Sixty-nine eyes of 56 patients undergoing penetrating keratoplasty were studied. Fifty-six age-matched normal eyes served as controls. Corneal epithelial structure was studied by using specular ...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00