Adherence of viable and nonviable bacteria to soft contact lenses.

Abstract:

:Bacterial viability in bacterial adherence to new soft contact lenses (SCLs) was studied by exposing polymacon and lidofilcon A lenses to Pseudomonas aeruginosa: L, live, untreated bacteria; H, bacteria heated for 1 h at 100 degrees C; and P, bacteria treated with 3% hydrogen peroxide for 1 h. Cultures of groups L, H, and P showed heavy, no, and minimal bacterial growth, respectively. Scanning electron microscopy (SEM) showed significantly less bacteria on new SCLs in groups H and P. Transmission electron microscopy (TEM) showed that bacterial shape was mostly preserved in all groups, with marked intracellular and cell membrane changes and breaks (groups H and P), margination of intracellular electron-dense material (group H), and central and peripheral vacuolation (group P). Adherence to new SCLs appears to be an active process, as killed or altered bacteria adhere less.

journal_name

Cornea

journal_title

Cornea

authors

John T,Refojo MF,Hanninen L,Leong FL,Medina A,Kenyon KR

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

21-33

issue

1

eissn

0277-3740

issn

1536-4798

journal_volume

8

pub_type

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