New Freeware for Image Analysis of Lissamine Green Conjunctival Staining.

Abstract:

PURPOSE:Lissamine green (LG) is often used in addition to fluorescein to assess the severity of conjunctival damage in dry eye syndrome, which is graded manually. Our purpose was to describe an algorithm designed for image analysis of LG conjunctival staining. METHODS:Twenty pictures of patients suffering from dry eye with visible LG conjunctival staining were selected. The images were taken by 2 different digital slit lamps with a white light source and a red filter transmitting over the wavelengths absorbed by LG. Conjunctival staining appeared in black on a red background. The red channel was extracted from the original image. Stained areas were then detected using a Laplacian of Gaussian filter and applying a threshold whose value was determined manually on a subset of images. The same algorithm parameters remained constant thereafter. LG-stained areas were also drawn manually by 2 experts as a reference. RESULTS:The delineation obtained by the algorithm closely matched the actual contours of the punctate dots. In 19 cases of 20 (95%), the algorithm found the same Oxford grade as the experts, even for confluent staining that was detected as a multitude of dots by the algorithm but not by the experts, resulting in a high overestimation of the total number of dots (without mismatching the Oxford grade estimated by the experts). The results were similar for the 2 slit-lamp imaging systems. CONCLUSIONS:This efficient new image-analysis algorithm yields results consistent with subjective grading and may offer advantages of automation and scalability in clinical trials.

journal_name

Cornea

journal_title

Cornea

authors

Courrier E,Renault D,Dib E,Urrea C,Kaspi M,Fournier C,Lépine T,Chiambaretta F,Hor G,He Z,Garcin T,Thuret G,Gain P

doi

10.1097/ICO.0000000000002617

subject

Has Abstract

pub_date

2020-12-18 00:00:00

eissn

0277-3740

issn

1536-4798

pii

00003226-900000000-95873

journal_volume

Publish Ahead of Print

pub_type

杂志文章

相关文献

CORNEA文献大全
  • A combined approach of amniotic membrane and oral mucosa transplantation for fornix reconstruction in severe symblepharon.

    abstract:PURPOSE:To evaluate the results of a combined approach of cicatrix lysis, intraoperative mitomycin C (MMC) application, oral mucosal transplantation (OMT), and amniotic membrane transplantation (AMT) for surgery of severe symblepharon. METHODS:This prospective study included 32 eyes with severe symblepharon in which a...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e318247983d

    authors: Kheirkhah A,Ghaffari R,Kaghazkanani R,Hashemi H,Behrouz MJ,Raju VK

    更新日期:2013-02-01 00:00:00

  • Frequency of positive donor rim cultures after penetrating keratoplasty using hypothermic and organ-cultured donor corneas.

    abstract:PURPOSE:To compare the frequency of positive rim cultures after penetrating keratoplasty using corneas preserved by hypothermic and organ culture storage. To evaluate the influence of standard procurement techniques on the frequency of microbial donor rim contamination. METHODS:Six hundred four donor corneas stored at...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3180415d7e

    authors: Fontana L,Errani PG,Zerbinati A,Musacchi Y,Di Pede B,Tassinari G

    更新日期:2007-06-01 00:00:00

  • Sulfur Hexafluoride 20% Versus Air 100% for Anterior Chamber Tamponade in DMEK: A Meta-Analysis.

    abstract:PURPOSE:To compare intracameral 20% sulfur hexafluoride (SF6) versus 100% air as tamponade for graft attachment in Descemet membrane endothelial keratoplasty (DMEK). METHODS:Using an electronic database search on MEDLINE and CENTRAL from inception to December 2017, we performed a literature review and meta-analysis in...

    journal_title:Cornea

    pub_type: 杂志文章,meta分析,评审

    doi:10.1097/ICO.0000000000001581

    authors: Marques RE,Guerra PS,Sousa DC,Ferreira NP,Gonçalves AI,Quintas AM,Rodrigues W

    更新日期:2018-06-01 00:00:00

  • Corneal endothelial cell damage after lens extraction using the fluid-based system compared to ultrasound phacoemulsification in human cadaver eyes.

    abstract:PURPOSE:To compare the endothelial cell damage after lens removal using a fluid-based system to conventional ultrasound. METHODS:Twenty human cadaver eyes were randomized to undergo phacoemulsification using either a fluid-based system or conventional ultrasound. After surgery, each corneoscleral button was removed, s...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/01.ico.0000130184.56514.51

    authors: Sandoval HP,Al Sarraf O,Vroman DT,Solomon KD

    更新日期:2004-10-01 00:00:00

  • Well-tolerated intracorneal wood foreign body of 40-year duration.

    abstract:PURPOSE:To report the diagnosis and management of a patient with a well-tolerated, long-standing retained intracorneal foreign body. METHODS:We describe the case of a 62-year-old man with a 40-year-old retained wood foreign body within the posterior stroma of the cornea. To the authors' knowledge, this is the longest ...

    journal_title:Cornea

    pub_type: 杂志文章,评审

    doi:10.1097/ICO.0b013e3181901e1f

    authors: Covert DJ,Henry CR,Sheth BP

    更新日期:2009-06-01 00:00:00

  • Corneal Endothelial Morphology and Thickness Alterations in Patients With Severe Obstructive Sleep Apnea-Hypopnea Syndrome.

    abstract:PURPOSE:To investigate central endothelial cell density (ECD), morphology, and central corneal thickness (CCT) in patients newly diagnosed with severe obstructive sleep apnea-hypopnea syndrome (OSAHS) and to determine the correlation between these parameters and OSAHS severity. METHODS:This prospective, comparative ca...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000002373

    authors: Chalkiadaki E,Andreanos K,Florou C,Droutsas K,Maniou C,Amfilochiou A,Georgalas I,Papaconstantinou D,Koutsandrea C

    更新日期:2021-01-01 00:00:00

  • Alkali Burn Over a LASIK Flap.

    abstract:PURPOSE:To describe the management and outcome of an ocular surface alkali burn in the setting of previous laser in situ keratomileusis (LASIK). METHODS:This is a case report and review of relevant literature. RESULTS:A 25-year-old man with a history of LASIK presented 4 weeks after a sodium hydroxide splash to his l...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000002604

    authors: Witsberger EM,Patel SV

    更新日期:2020-12-03 00:00:00

  • Three-dimensional optical coherence tomography of granular corneal dystrophy.

    abstract:PURPOSE:To describe 3-dimensional optical coherence tomography (OCT) findings in a case of granular corneal dystrophy. METHODS:A 1310-nm high-speed swept-source OCT prototype was used to image the 3-dimensional structures of the cornea of a patient with granular corneal dystrophy. RESULTS:Three-dimensional OCT clearl...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31802e1e50

    authors: Miura M,Mori H,Watanabe Y,Usui M,Kawana K,Oshika T,Yatagai T,Yasuno Y

    更新日期:2007-04-01 00:00:00

  • The change in ocular higher-order aberrations after pterygium excision with conjunctival autograft: a 1-year prospective clinical trial.

    abstract:PURPOSE:To investigate the effects of pterygium excision with conjunctival autograft plus corneal polishing on ocular higher-order aberrations (HOAs) and to monitor the changes throughout the first postoperative year. METHODS:A total of 18 eyes of 16 patients with primary pterygium who had an indication for surgical e...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3182431465

    authors: Gumus K,Topaktas D,Göktaş A,Karakucuk S,Oner A,Mirza GE

    更新日期:2012-12-01 00:00:00

  • Assessment of keratocyte density in patients with keratoconus not using contact lenses.

    abstract:PURPOSE:To assess the keratocyte density (KD) in the cornea of patients with keratoconus not using contact lenses. METHOD:In this pilot study, the eyes (n = 68) of 35 patients with the diagnosis of keratoconus, who did not use contact lenses and the eyes(n = 70) of 35 healthy controls were prospectively examined using...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3182000fd4

    authors: Timucin OB,Karadag MF,Cinal A

    更新日期:2011-05-01 00:00:00

  • Transmission of rabies by corneal graft.

    abstract::Viruses such as human immunodeficiency virus, herpes simplex virus, cytomegalovirus, hepatitis B, hepatitis C, and rabies can be transmitted to the recipient by corneal transplantation. Very few cases of rabies have been reported. Here we report two cases of rabies transmitted by the same donor. Both of the recipients...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-199607000-00014

    authors: Javadi MA,Fayaz A,Mirdehghan SA,Ainollahi B

    更新日期:1996-07-01 00:00:00

  • Expression of p63 in pterygium and normal conjunctiva.

    abstract:PURPOSE:The p63 gene has been identified as a marker of epithelial stem cells. Because pterygium may arise through an expansion of the proliferative capacity of the conjunctiva, we sought to document the expression of p63 in normal conjunctiva and pterygium specimens. METHODS:Immunostaining for p63 expression was perf...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-200401000-00011

    authors: Sakoonwatanyoo P,Tan DT,Smith DR

    更新日期:2004-01-01 00:00:00

  • Long-term clinical course of dry eye in patients with chronic graft-versus-host disease referred for eye examination.

    abstract:PURPOSE:To assess the long-term clinical course of dry eye in patients with chronic graft-versus-host disease (cGVHD). METHODS:A prospective case series of 49 patients with cGVHD was conducted. Complete history and ophthalmic examination were performed at baseline and at 36 months (range, 26-53). All patients received...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3181e9b3bf

    authors: Sáles CS,Johnston LJ,Ta CN

    更新日期:2011-02-01 00:00:00

  • Multirecurrence of corneal posterior polymorphous dystrophy. An ultrastructural study.

    abstract::Posterior polymorphous corneal dystrophy (PPD) is a rare bilateral, autosomal-dominant disease. The presence of the epitheliumlike endothelium and the thickening of Descemet's membrane by the posterior collagenous layer (PCL) has been reported in the majority of published cases. Reepithelialization of the posterior co...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:

    authors: Sekundo W,Lee WR,Aitken DA,Kirkness CM

    更新日期:1994-11-01 00:00:00

  • High-Resolution Optical Coherence Tomography Findings of Lisch Epithelial Corneal Dystrophy.

    abstract:PURPOSE:To describe a case of Lisch epithelial corneal dystrophy (LECD) and present its unique characteristics on high-resolution optical coherence tomography (HR-OCT). METHODS:A 78-year-old man with whorled corneal epithelial opacities in the right eye was referred for the evaluation of ocular surface squamous neopla...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000722

    authors: Pole C,Sise A,Joag M,Galor A,Bermudez-Magner JA,Dubovy S,Karp CL

    更新日期:2016-03-01 00:00:00

  • Treatment of advanced acanthamoeba keratitis with deep lamellar keratectomy and conjunctival flap.

    abstract:PURPOSE:To describe whether deep lamellar keratectomy with a conjunctival flap is effective for the treatment of Acanthamoeba keratitis. METHODS:Two patients (three eyes) had at least a 4-week history of painful keratitis misdiagnosed as herpetic keratitis and bacterial keratitis. Both patients were started on multipl...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-200210000-00014

    authors: Cremona G,Carrasco MA,Tytiun A,Cosentino MJ

    更新日期:2002-10-01 00:00:00

  • Descemet Membrane Endothelial Keratoplasty in Children.

    abstract:PURPOSE:To describe the outcomes of Descemet membrane endothelial keratoplasty (DMEK) performed in amblyopic aged (younger than 8 years) children. METHODS:This is a single-center retrospective study, including 11 eyes (7 congenital hereditary endothelial dystrophy and 4 congenital glaucoma) of 6 children in amblyopic ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000002540

    authors: Pereira NC,Pereira Gomes JÁ,Tonin C,Verardo FO,Felippe RS,Dos Santos Forseto A

    更新日期:2020-09-16 00:00:00

  • Corneal lymphangiogenesis: evidence, mechanisms, and implications for corneal transplant immunology.

    abstract:PURPOSE:The normal cornea is devoid of blood and lymphatic vessels but can become vascularized secondary to a variety of corneal diseases and surgical manipulations. Whereas corneal (hem)angiogenesis, i.e., the outgrowth of new blood vessels from preexisting limbal vessels, is obvious both clinically and histologically...

    journal_title:Cornea

    pub_type: 杂志文章,评审

    doi:10.1097/00003226-200304000-00021

    authors: Cursiefen C,Chen L,Dana MR,Streilein JW

    更新日期:2003-04-01 00:00:00

  • Keratinizing corneal intraepithelial neoplasia.

    abstract::Corneal intraepithelial neoplasia (CIN) is the term applied by some authors to the spectrum of disease ranging from mild dysplasia to carcinoma in situ. Such lesions usually are associated with dysplastic or neoplastic processes at the limbus or adjacent conjunctiva; isolated corneal dysplasia is rare. Clinically, CIN...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:

    authors: Brown HH,Glasgow BJ,Holland GN,Foos RY

    更新日期:1989-09-01 00:00:00

  • Outcomes of repeat endothelial keratoplasty in patients with failed deep lamellar endothelial keratoplasty.

    abstract:PURPOSE:To report the outcomes of repeat endothelial keratoplasty (EK) in patients with previous failed deep lamellar endothelial keratoplasty (DLEK). METHODS:This retrospective interventional case series involved the review of clinical records of patients with failed DLEK surgery who underwent repeat EK surgery. RES...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31821522b4

    authors: Kim P,Brodbaker E,Lichtinger A,Yeung SN,Bahar I,Iovieno A,Amiran MD,Rootman DS

    更新日期:2011-11-01 00:00:00

  • Nineteen years of penetrating keratoplasty in the Hotel-Dieu Hospital in Paris.

    abstract:PURPOSE:To identify changing trends in penetrating keratoplasties (PKs) performed at the Hotel-Dieu Hospital in Paris between January 1980 and December 1999 and to explain the reasons for the changes. METHODS:We retrospectively reviewed 3,736 of the 3,836 PKs performed between January 1, 1980, and December 31, 1999, a...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-200108000-00009

    authors: Legeais JM,Parc C,d'Hermies F,Pouliquen Y,Renard G

    更新日期:2001-08-01 00:00:00

  • Effectiveness of ciprofloxacin and ofloxacin in a prophylaxis model of Staphylococcus keratitis.

    abstract:PURPOSE:To determine the effectiveness of prophylactic fluoroquinolone treatment against staphylococci in a rabbit keratitis model. METHODS:Prophylactic ciprofloxacin or ofloxacin was applied as one topical drop 15 minutes before infection or as one drop at three time points (19, 17, and 15 minutes) before infection. ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-200111000-00020

    authors: Dajcs JJ,Moreau JM,Thibodeaux BA,Traidej M,Austin MS,Marquart ME,Stroman DW,O'Callaghan RJ

    更新日期:2001-11-01 00:00:00

  • Fellow Eye Comparison of Descemet Membrane Endothelial Keratoplasty and Penetrating Keratoplasty.

    abstract:PURPOSE:To compare the visual outcomes and postoperative complications in patients undergoing penetrating keratoplasty (PKP) in 1 eye followed by Descemet membrane endothelial keratoplasty (DMEK) in their fellow eye. METHODS:A retrospective analysis of 11 patients, who underwent a PKP procedure first in 1 eye and then...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31829dd816

    authors: Maier AK,Gundlach E,Gonnermann J,Klamann MK,Eulufi C,Bertelmann E,Joussen AM,Torun N

    更新日期:2013-10-01 00:00:00

  • Delayed, bilateral descemet's membrane detachments with spontaneous resolution: Implications for nonsurgical treatment.

    abstract:PURPOSE:To report a case of delayed bilateral Descemet's detachment with spontaneous resolution in one eye following successful treatment of the fellow eye. METHOD:Case report. RESULTS:A 78-year-old male who underwent uncomplicated bilateral cataract extraction with phacoemulsification developed delayed bilateral Des...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e318197eef1

    authors: Couch SM,Baratz KH

    更新日期:2009-12-01 00:00:00

  • Effect of intravitreal ranibizumab on corneal endothelium in age-related macular degeneration.

    abstract:PURPOSE:To determine the effect of intravitreal injection of ranibizumab on the corneal endothelium in patients with choroidal neovascularization in age-related macular degeneration. METHODS:Observational prospective case series study. Fifty-two eyes of 52 consecutive patients (29 men, 23 women; age range, 61-80 years...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3181ca33d2

    authors: Pérez-Rico C,Benítez-Herreros J,Castro-Rebollo M,Gómez-Sangil Y,Germain F,Montes-Mollón MA,Teus MA

    更新日期:2010-08-01 00:00:00

  • A Prospective, In Vitro, Randomized Study to Compare Two Media for Donor Corneal Storage.

    abstract:PURPOSE:To determine if Cornisol is as effective as Optisol-GS as an intermediate-term corneal storage medium. METHODS:A prospective, in vitro, randomized, partially masked study using paired donor corneas. Optical grade tissues not suitable for surgery were used. After performing baseline slit lamp evaluation, one ti...

    journal_title:Cornea

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/ICO.0000000000000912

    authors: Basak S,Prajna NV

    更新日期:2016-09-01 00:00:00

  • Ultraviolet A/riboflavin corneal cross-linking for infectious keratitis associated with corneal melts.

    abstract:PURPOSE:To evaluate the efficacy of ultraviolet-corneal cross-linking (CXL) for treating infectious melting keratitis. METHODS:Five patients with infectious keratitis associated with corneal melting were treated with CXL at the outpatient departments of the Institut für Refraktive und Ophthalmo-Chirurgie and the eye h...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e318169d698

    authors: Iseli HP,Thiel MA,Hafezi F,Kampmeier J,Seiler T

    更新日期:2008-06-01 00:00:00

  • Adherence of viable and nonviable bacteria to soft contact lenses.

    abstract::Bacterial viability in bacterial adherence to new soft contact lenses (SCLs) was studied by exposing polymacon and lidofilcon A lenses to Pseudomonas aeruginosa: L, live, untreated bacteria; H, bacteria heated for 1 h at 100 degrees C; and P, bacteria treated with 3% hydrogen peroxide for 1 h. Cultures of groups L, H,...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:

    authors: John T,Refojo MF,Hanninen L,Leong FL,Medina A,Kenyon KR

    更新日期:1989-01-01 00:00:00

  • Topographic and biomechanical evaluation of cornea in patients with acromegaly.

    abstract:PURPOSE:The aim of this study was to compare topographic and biomechanical properties of corneas in patients with acromegaly with those of healthy individuals. METHODS:Thirty-five patients with acromegaly (study group) and 35 healthy individuals (control group) were enrolled in this prospective study. Topographic meas...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000297

    authors: Altinkaynak H,Duru N,Ersoy R,Kalkan Akcay E,Ugurlu N,Cagil N,Cakir B

    更新日期:2015-01-01 00:00:00

  • The effect of aqualase and phacoemulsification on the corneal endothelium.

    abstract:PURPOSE:To compare corneal endothelial cell loss after cataract surgery by phacoemulsification and by Aqualase. METHODS:This was a prospective, randomized study of 75 eyes of 75 patients undergoing cataract surgery. Patients were randomly assigned to receive either phacoemulsification or Aqualase for cataract removal....

    journal_title:Cornea

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/ICO.0b013e3181af7c43

    authors: Hu V,Hughes EH,Patel N,Whitefield LA

    更新日期:2010-03-01 00:00:00