Abstract：
:In vitro investigations of cell-specific metabolism and cell interactions as well as biocompatibility studies are often hampered by the limited lifespan of primary cells originating from target tissues like the oral mucosa, gingiva or pulp. Pulp cells, as do other primary cells, undergo senescence after several passages in vitro. However, senescence can be overcome by transfection of primary cells with oncogenes like the HPV 18 (human papillomavirus 18) E6/E7 oncogene, resulting in immortalized cell lines. The purpose of our study was to establish and preliminary characterize an immortalized bovine dental papilla-derived cell line by transfection with HPV 18 E6/E7 for future use in biocompatibility testing of dental materials. First passage dental papilla-derived cells from molar tooth germs of 6-month-old calves were transfected by electroporation with pUC18 LCR E6/E7 coding for the HPV 18 E6/E7 oncogene. Cells underwent crisis after 5 wk in culture. Distinct cell colonies arose after about 9 wk. Cells were cloned by single cell dilution in passage 15 and 17. Out of three cell clones maintained in culture, two cell clones showed cell death after 28 and 30 wk, respectively. One cell clone overcame a second crisis after 38 wk and was maintained in culture until passage 75. Stable gene expression of HPV 18 E6/E7 oncogenes was verified by polymerase chain reaction (PCR) and immunohistochemistry. Reverse transcription (RT)-PCR revealed that the established cell line (passage 50) expresses procollagen type I, alkaline phosphatase and osteocalcin. This suggests that the immortalization with HPV 18 E6/E7 results in a cell line, which maintained phenotypic characteristics of odontoblast-like cells.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Thonemann B,Schmalz Gdoi
10.1034/j.1600-0722.2000.108005432.xsubject
Has Abstractpub_date
2000-10-01 00:00:00pages
432-41issue
5eissn
0909-8836issn
1600-0722journal_volume
108pub_type
杂志文章abstract：:Mandibular movements occur through the triggering of trigeminal motoneurons. Aberrant movements by orofacial muscles are characteristic of orofacial motor disorders, such as nocturnal bruxism (clenching or grinding of the dentition during sleep). Previous studies have suggested that autonomic changes occur during brux...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2009.00686.x
更新日期：2009-12-01 00:00:00
abstract：:Paraffin-stimulated saliva samples were collected from 140 children 3- and 4-yr old attending nine nursery schools in Latvia. The salivary levels of mutans streptococci were rated from zero to 3 after being cultured on a commercially available strip selective for these microorganisms. Of the children, 29.3% were rated...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1995.tb00171.x
更新日期：1995-08-01 00:00:00
abstract：:Mutation of the dihydroorotate dehydrogenase (DHODH) gene is responsible for Miller syndrome, which is characterized by craniofacial malformations with limb abnormalities. We previously demonstrated that DHODH was involved in forming a mitochondrial supercomplex and that mutated DHODH led to protein instability, loss ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12270
更新日期：2016-06-01 00:00:00
abstract：:The objectives of this study were to determine the prevalence, intensity, and extent of the impacts caused by oral problems, and to compare their intensity and extent by the type of oral problem. Four of seven public schools linked to a Health Center in Lima, Peru, were randomly selected as clusters, and 805 11-12-yr-...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2007.00440.x
更新日期：2007-04-01 00:00:00
abstract：:Ozone gas and photodynamic therapy (PDT) have been claimed to be antimicrobially effective. This study assessed their antimicrobial potential in vitro. Mature six-species oral biofilms were treated as follows (n = 9 per group): (i) a 60-s application of gasiform vacuum-ozone or vacuum alone (on wet or air-dried biofil...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2007.00418.x
更新日期：2007-02-01 00:00:00
abstract：:Amelogenesis imperfecta is a group of hereditary enamel defects. Of the autosomal dominant forms, only the local hypoplastic type has been mapped to human chromosome 4q 13-4q21. Enamelin is a large enamel matrix protein secreted by ameloblasts. The purpose of this study was to determine the human chromosomal localizat...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108005353.x
更新日期：2000-10-01 00:00:00
abstract：:This study investigated the importance of dental beliefs and the predictive value of the Dental Belief Survey (DBS) in dental-fear treatment. The sample comprised 117 adult patients seeking treatment at a dental-fear clinic. Pretreatment data were collected during a screening procedure, including two visits to the den...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2003.00016.x
更新日期：2003-04-01 00:00:00
abstract：:Patients with cardiac implantable electrical devices should take precautions when exposed to electromagnetic fields. Possible interference as a result of proximity to electromagnets or electricity flow from electronic tools employed in clinical odontology remains controversial. The objective of this study was to exami...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12183
更新日期：2015-06-01 00:00:00
abstract：:This study measured the polymerization contraction of a resin composite cured at three levels of energy density, each attained at six different levels of power density. The polymerization contraction of the composite was recorded by the method of the deflecting disc (n = 5) for 1 h following the start of irradiation. ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2005.00239.x
更新日期：2005-10-01 00:00:00
abstract：:The aim of this study was to visualize fluid movement through dental enamel in vivo. Fifty permanent upper central incisors, from subjects aged 10-70 yr, and 5 permanent central just-erupted incisors, from subjects aged 6-7 yr, were included in the study. An impression was obtained by vinyl polyxiloxane, and replicas ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2007.00445.x
更新日期：2007-06-01 00:00:00
abstract：:The purpose was to investigate if antigenic fragments of aggrecan and cartilage oligomeric matrix protein (COMP) are detectable by enzyme-linked immunosorbent assay in lavage fluids from the temporomandibular joint (TMJ) and to examine if the relative content of these cartilage markers changes during development of os...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00254.x
更新日期：1997-08-01 00:00:00
abstract：:Current interest in dental erosion has led to increasing attention to ways in which potentially erosive products might be modified. Information on how this could be achieved has been hard to gather, and has focused chiefly on possibilities in reformulating soft drinks. The bulk of the work published on this relates to...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0722.1996.tb00071.x
更新日期：1996-04-01 00:00:00
abstract：:Kallikrein-related peptidase 4 (KLK4) is critical for proper dental enamel formation. Klk4 null mice, and humans with two defective KLK4 alleles have obvious enamel defects, with no other apparent phenotype. KLK4 mRNA or protein is reported to be present in tissues besides teeth, including prostate, ovary, kidney, liv...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00834.x
更新日期：2011-12-01 00:00:00
abstract：:The outer membrane of Porphyromonas gingivalis, an oral strict anaerobe, was isolated by sucrose density gradient centrifugation. The outer membrane obtained by the differential detergent extraction method, previously reported, showed an essentially similar protein pattern on sodium dodecyl sulfate-polyacrylamide gel ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2002.11171.x
更新日期：2002-04-01 00:00:00
abstract：:Vitamin D acts through binding with vitamin D receptor (VDR) and is responsible for regulating bone metabolism and mineralization; it also suppresses the immune system. The aim of this study was to investigate if VDR gene polymorphisms are associated with chronic periodontitis (CP) and aggressive periodontitis (AgP) i...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12085
更新日期：2013-12-01 00:00:00
abstract：:The expression of HLA Class II antigens on the surfaces of immunocompetent cells and the presence of CD1a+ cells (Langerhans cells) are important components of antigen presentation. Quantitative variations in HLA class II expression on antigen-presenting cells play a role in immune regulation. An indirect immunofluore...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1995.tb00161.x
更新日期：1995-08-01 00:00:00
abstract：:Our previous study showed that p53 protein is accumulated in >60% of cases of oral squamous cell carcinoma (OSCC) and its overexpression is related to poor prognosis. However, the mechanism behind this is still elusive. The present study attempts to dissect p53 alterations at various levels from gene to function in tu...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12007
更新日期：2013-02-01 00:00:00
abstract：:Women from ancient societies have shown a higher prevalence of dental caries in comparison with men. Recent research has shown that the relationship between increased oestrogen production during pregnancy and decreased salivary flow is a possible cause for the higher levels of caries in women, which is in contrast to ...
journal_title：European journal of oral sciences
pub_type： 历史文章,杂志文章
doi：10.1111/eos.12586
更新日期：2019-02-01 00:00:00
abstract：:Parathyroid hormone-related peptide (PTHrP) was discovered as the tumor product that is responsible for most instances of the syndrome of humoral hypercalcemia of malignancy. It is now known that the PTHrP and PTH genes arose on the basis of an ancient duplication event. One result of this heritage is a short stretch ...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0722.1998.tb02150.x
更新日期：1998-01-01 00:00:00
abstract：:Recent thinking about the role of regional socioeconomic context in generating health inequalities has argued for the collection of true 'area' data that accurately reflect the characteristics of a region. We investigated whether a range of attributes of regional environments, constructed from factor analysis of vario...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00831.x
更新日期：2011-08-01 00:00:00
abstract：:This study evaluated the effect of topical fluoride application on enamel hardness after in-office bleaching. Twelve human incisors were cut along the long axis, resulting in 24 halves used in four treatment groups (n = 6 in each group): (i) untreated group (C); (ii) in-office bleaching material (B); (iii) treatment w...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12386
更新日期：2017-12-01 00:00:00
abstract：:To explore the intrafamilial homology and longitudinal stability of colonization by early acquired mutans streptococci, genomic fingerprinting was performed on 254 strains (192 Streptococcus mutans and 62 Streptococcus sobrinus strains) collected from 16 families (16 mother-child pairs, seven fathers and four siblings...
journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1034/j.1600-0722.2003.00068.x
更新日期：2003-10-01 00:00:00
abstract：:Temporomandibular joint (TMJ) histology was investigated in 8 female Scandinavian moose. 5 were 1-year-old with a carcass weight (cw) of 125-140 kg, and 3 were 2-years-old (160-175 kg cw). The condylar articular surface consisted of a connective tissue lining with parallel collagen fibres. Numerous blood vessels were ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00146.x
更新日期：1996-10-01 00:00:00
abstract：:The objective of this clinical study was to assess the internal and marginal accuracy of computer-aided design/computer-aided manufacturing (CAD/CAM)-generated four-unit all-ceramic posterior fixed dental prostheses (FDPs). The data were compared with the results of three-unit metal-ceramic and all-ceramic FDPs that w...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00580.x
更新日期：2008-12-01 00:00:00
abstract：:This study evaluated the effect of core-design modification on the characteristic strength and failure modes of glass-infiltrated alumina (In-Ceram) (ICA) compared with porcelain fused to metal (PFM). Premolar crowns of a standard design (PFMs and ICAs) or with a modified framework design (PFMm and ICAm) were fabricat...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00608.x
更新日期：2009-04-01 00:00:00
abstract：:The skeletal remains of Giovanna of Austria (1547-1578), daughter of the Emperor Ferdinand I of Habsburg (1503-1564) and first wife of the Grand Duke of Tuscany, Francesco I (1541-1587), exhumed from the Basilica of San Lorenzo in Florence, were submitted to paleopathological study. Examination of the dentition, which...
journal_title：European journal of oral sciences
pub_type： 传,历史文章,杂志文章
doi：10.1111/eos.12097
更新日期：2014-02-01 00:00:00
abstract：:The aim of this study was to explore a possible association between health status and self-reported adverse effects related to dental amalgam restorations. A group of 50 consecutive patients (index group), referred for complaints self-related to dental amalgam restorations, was compared with a control group of individ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00206.x
更新日期：1997-06-01 00:00:00
abstract：:Orthodontic therapy is known to have an aggravating effect on the progression of destructive periodontitis if oral hygiene is not maintained. However, it is largely unknown how active periodontitis affects the velocity of orthodontic tooth movement. In this study, we examined the effect of periodontal inflammation on ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2009.00625.x
更新日期：2009-06-01 00:00:00
abstract：:The aim of this study was to determine the relative contribution of changes (design factors) in implant system, position, bone classification, and loading condition on the biomechanical response of a single-unit implant-supported restoration. Non-linear finite-element analysis was used to simulate the mechanical respo...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2007.00473.x
更新日期：2007-10-01 00:00:00
abstract：:To investigate change in oral health in relation to use of dental services, a random sample of 45- to 54-yr-old subjects from Adelaide, South Australia, was surveyed in 2004-2005 (n = 986, response rate = 44.4%). Service use and a global oral-health transition (GOHT) statement were collected over 2 yr. Worsening in or...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2012.00985.x
更新日期：2012-10-01 00:00:00
