Abstract：
:Mutation of the dihydroorotate dehydrogenase (DHODH) gene is responsible for Miller syndrome, which is characterized by craniofacial malformations with limb abnormalities. We previously demonstrated that DHODH was involved in forming a mitochondrial supercomplex and that mutated DHODH led to protein instability, loss of enzyme activity, and increased levels of reactive oxygen species in HeLa cells. To explore the etiology of Miller syndrome in more detail, we investigated the effects of DHODH inhibition in the cells involved in skeletal structure. Dihydroorotate dehydrogenase in MC3T3-E1 cells derived from mouse calvaria osteoblast precursor cells was knocked down by specific small interfering RNAs (siRNAs), and cell proliferation, ATP production, and expression of bone-related genes were investigated in these cells. After depletion of DHODH using specific siRNAs, inhibition of cell proliferation and cell cycle arrest occurred in MC3T3-E1 cells. In addition, ATP production was reduced in whole cells, especially in mitochondria. Furthermore, the levels of runt-related transcription factor 2 (Runx2) and osteocalcin (Ocn) mRNAs were lower in DHODH siRNA-treated cells compared with controls. These data suggest that depletion of DHODH affects the differentiation and maturation of osteoblasts. This study shows that mitochondrial dysfunction by DHODH depletion in osteoblasts can be directly linked to the abnormal bone formation in Miller syndrome.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Fang J,Yamaza H,Uchiumi T,Hoshino Y,Masuda K,Hirofuji Y,Wagener FA,Kang D,Nonaka Kdoi
10.1111/eos.12270subject
Has Abstractpub_date
2016-06-01 00:00:00pages
241-5issue
3eissn
0909-8836issn
1600-0722journal_volume
124pub_type
杂志文章abstract：:The aim of this study was to evaluate the postoperative consequences of chorda tympani reclining during middle ear surgery for otosclerosis. Electrogustometric taste thresholds were measured at 11 loci on the tongue and the soft palate in 14 patients before surgery, and 8 d, 1 month and (in some cases) 6 months after ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00556.x
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abstract：:Periodontitis progresses due to increased levels of active metalloproteinases (MMPs) and the imbalance between MMPs and their tissue inhibitors (TIMPs). Natural curcumin limits the lytic activity of MMPs but has low cellular uptake. Use of synthetic curcumin analogs could be a means of overcoming this limitation of tr...
journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12135
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journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
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abstract：:Because low-fluoride toothpaste (500 p.p.m. F) has not clearly been shown to be effective for controlling caries in caries-active children, this experimental in situ study was conducted to evaluate whether its effect, when compared with a conventional toothpaste (1,000-1,500 p.p.m. F), would depend on the cariogenic c...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2003.00036.x
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journal_title：European journal of oral sciences
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pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.0909-8836.2003.00078.x
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12007
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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更新日期：2005-04-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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abstract：:The aim of this study was to determine the effect of interleukin-10 (IL-10) on the gene expression of osteoclastogenic regulatory molecules in rat dental follicle cells. Interleukin-10 is an anti-inflammatory cytokine that inhibits alveolar bone resorption, but the molecular basis for this is unknown. Alveolar bone re...
journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章
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更新日期：1995-12-01 00:00:00
abstract：:We sought to investigate site-specific expression of bone-regulatory factors expressed by human dental follicles and to compare the stimulated expression of tumour necrosis factor (ligand) superfamily, member 11/tumour necrosis factor receptor superfamily, member 11b (RANKL/OPG) in human dental follicle cells (HDFCs) ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.0909-8836.2003.00076.x
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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