Abstract：
:We sought to investigate site-specific expression of bone-regulatory factors expressed by human dental follicles and to compare the stimulated expression of tumour necrosis factor (ligand) superfamily, member 11/tumour necrosis factor receptor superfamily, member 11b (RANKL/OPG) in human dental follicle cells (HDFCs) from different patients. Analysis of bone-regulatory markers in follicles from 12 different study participants was performed using RT-qPCR and immunofluorescence; apical and coronal segments from each dental follicle were processed independently. Four additional dental follicles were used for cell cultures; HDFCs were precultured in osteogenic medium to initiate differentiation and thereafter cultured with 10-6 M forskolin (FSK) to activate the protein kinase cAMP (PKA/cAMP) signalling pathway and induce RANKL/OPG expression. We demonstrate that RANKL expression is significantly higher in the coronal part of follicles than in the apical part. High levels of collagen type 1 (COL1), alkaline phosphatase (ALP) and Gap-junction protein, alpha 1, 43 kDa (CX43) were expressed, whereas expression of Sp7 transcription factor (OSX), bone morphogenetic protein 2 (BMP2), colony-stimulating factor 1 (CSF-1), chemokine (C-C motif) ligand 2 (MCP1), and OPG was low in all samples. The immunofluorescence localization of CSF-1, MCP1, osteocalcin (OCN), RANKL, and BMP2 was not specific for either part of the follicles. In conclusion, a consistently high expression of CX43 suggests that gap-junction communication in HDFCs is essential for the eruption process. Furthermore, the induced expression of RANKL in HDFCs varies significantly between individuals and may relate to clinical variations in tooth eruption.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Uribe P,Plakwicz P,Larsson L,Czochrowska E,Westerlund A,Ransjö Mdoi
10.1111/eos.12568subject
Has Abstractpub_date
2018-12-01 00:00:00pages
439-448issue
6eissn
0909-8836issn
1600-0722journal_volume
126pub_type
杂志文章abstract：:The skeletal remains of Giovanna of Austria (1547-1578), daughter of the Emperor Ferdinand I of Habsburg (1503-1564) and first wife of the Grand Duke of Tuscany, Francesco I (1541-1587), exhumed from the Basilica of San Lorenzo in Florence, were submitted to paleopathological study. Examination of the dentition, which...
journal_title：European journal of oral sciences
pub_type： 传,历史文章,杂志文章
doi：10.1111/eos.12097
更新日期：2014-02-01 00:00:00
abstract：:Orthodontic therapy is known to have an aggravating effect on the progression of destructive periodontitis if oral hygiene is not maintained. However, it is largely unknown how active periodontitis affects the velocity of orthodontic tooth movement. In this study, we examined the effect of periodontal inflammation on ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2009.00625.x
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abstract：:Xylitol reduces plaque but the reduction mechanism is largely unknown. The main aim of the present study was to determine whether the xylitol-induced reduction in the amount of plaque and the number of mutans streptococci could be demonstrated in subjects with (presumably) high levels of xylitol-resistant (XR; not inh...
journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1111/j.1600-0722.1997.tb00196.x
更新日期：1997-04-01 00:00:00
abstract：:Osteoclasts are hematopoietic in origin and formed by proliferation, differentiation and fusion of osteoclast progenitor cells. However, the signal transducing mechanisms involved in generation of osteoclasts are not clear. We have used two well-known adenylate cyclase stimulators to examine the effect of cyclic AMP (...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1999.eos107108.x
更新日期：1999-02-01 00:00:00
abstract：:Formation of crystals in the enamel space releases protons that need to be buffered to sustain mineral accretion. We hypothesized that apical cystic fibrosis transmembrane conductance regulator (CFTR) in maturation ameloblasts transduces chloride into forming enamel as a critical step to secrete bicarbonates. We teste...
journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.0909-8836.2004.00098.x
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abstract：:The objective of this study was to obtain a geographic world map of scientific production in dentistry by analysing published papers. Articles and reviews in the Dentistry, Oral Surgery & Medicine category published from 1999 to 2003 were accessed through the ISI database. The data were analyzed quantitatively (number...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2006.00244.x
更新日期：2006-04-01 00:00:00
abstract：:Subsurface demineralization in enamel caries is known to entail diffusion of reagents and products both within the lesion and within the plaque biofilm external to the lesion. However, development of a predictive mathematical model for subsurface demineralization is hindered by limited quantitative understanding of th...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2005.00188.x
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abstract：:To explore the intrafamilial homology and longitudinal stability of colonization by early acquired mutans streptococci, genomic fingerprinting was performed on 254 strains (192 Streptococcus mutans and 62 Streptococcus sobrinus strains) collected from 16 families (16 mother-child pairs, seven fathers and four siblings...
journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1034/j.1600-0722.2003.00068.x
更新日期：2003-10-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.00767.x
更新日期：2000-02-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2006.00314.x
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abstract：:Human dentin mineral has been investigated by using micro-Raman spectroscopy. Fluorescence and thermal problems were largely avoided by preparing dentin samples by grinding and ultrasonic agitation in acetone. The Raman spectral features were consistent with those of impure hydroxyapatite containing CO3 and HPO4. Whil...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00056.x
更新日期：1996-04-01 00:00:00
abstract：:Inositol polyphosphate 4-phosphatase type II (INPP4B) is a phosphoinositide phosphatase that plays complex roles in the pathogenesis of different tumors. We aimed to explore the expression, clinicopathological significance, and prognostic value of INPP4B in oral squamous cell carcinoma (OSCC). Tissue microarrays that ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12673
更新日期：2020-02-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00206.x
更新日期：1997-06-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2011.00894.x
更新日期：2012-02-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00814.x
更新日期：2011-04-01 00:00:00
abstract：:A derivative (EMD) of enamel matrix proteins (EMPs) is used for periodontal regeneration because EMPs are believed to induce the formation of acellular extrinsic fiber cementum (AEFC). Other reports, however, indicate that EMPs have osteogenic potential. The aim of this study was to characterize the nature of the tiss...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2006.00300.x
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2005.00225.x
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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更新日期：2014-12-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12386
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00888.x
更新日期：2011-12-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12270
更新日期：2016-06-01 00:00:00
abstract：:Secretion rates and composition of unstimulated and chewing-stimulated whole saliva and 3% citric acid stimulated parotid and submandibular-sublingual secretions were studied in 12 hypertensive patients during withdrawal of and re-exposure to antihypertensive pharmacotherapy. All the patients' blood pressures were wel...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00076.x
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abstract：:There is limited empirical information as to whether or how stimuli associated with dental fear can be classified into distinct subtypes. The purpose of the current study was to develop a descriptive framework for the classification of dental fear. Data were collected using a survey among Dutch twin families (n = 11,7...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12343
更新日期：2017-06-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00156.x
更新日期：1996-10-01 00:00:00
abstract：:Kallikrein-related peptidase 4 (KLK4) is critical for proper dental enamel formation. Klk4 null mice, and humans with two defective KLK4 alleles have obvious enamel defects, with no other apparent phenotype. KLK4 mRNA or protein is reported to be present in tissues besides teeth, including prostate, ovary, kidney, liv...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00834.x
更新日期：2011-12-01 00:00:00
abstract：:Fluoride is associated with a decrease in the incidence of dental caries, but excessive fluoride intake during tooth enamel formation can result in enamel fluorosis. Fluorosed enamel has increased porosity, which has been related to a delay in the removal of amelogenin proteins as the enamel matures. This delay in pro...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2006.00303.x
更新日期：2006-05-01 00:00:00
