Abstract:
:Osteoclasts are hematopoietic in origin and formed by proliferation, differentiation and fusion of osteoclast progenitor cells. However, the signal transducing mechanisms involved in generation of osteoclasts are not clear. We have used two well-known adenylate cyclase stimulators to examine the effect of cyclic AMP (cAMP) on the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells in cultured mouse calvarial bones and in mouse bone marrow cultures. The effects of forskolin and cholera toxin were compared with those of parathyroid hormone (PTH) and 1,25(OH)2vitaminD3 (1,25(OH)2D3). PTH, as well as forskolin and cholera toxin, increased the number of osteoclast profiles/mm bone in 24-h and 120-h cultures of mouse calvarial bones. In mouse bone marrow cultures, 1,25(OH)2D3 or PTH stimulated formation of TRAP-positive multinucleated cells. Moreover, forskolin or cholera toxin produced dose-dependent stimulation of these cells at a range of concentrations correlating with their effect on cAMP production. The osteoclastic phenotype of the TRAP-positive cells was demonstrated by autoradiography of 125I-labelled calcitonin binding and by the bone-resorbing activity of the cells. The sustained presence (0-9 d) of forskolin or PTH was required to obtain maximal formation of osteoclasts. However, the presence of 1,25(OH)2D3 was required only for the last 3 d of culture for maximal osteoclast formation. We conclude that PTH may stimulate osteoclast generation using the adenylate cyclase cAMP system as a signal transduction mechanism.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Ransjö M,Lie A,Mackie EJdoi
10.1046/j.0909-8836.1999.eos107108.xsubject
Has Abstractpub_date
1999-02-01 00:00:00pages
45-54issue
1eissn
0909-8836issn
1600-0722journal_volume
107pub_type
杂志文章abstract::Granulocyte elastase was determined in the gingival crevicular fluid (GCF) of 18 periodontitis patients. They initially had similar severity of disease but had responded differently to 5-yr maintenance, 13 responders and 5 non-responders. A total of 102 sites were investigated and categorized as: i) consistently healt...
journal_title:European journal of oral sciences
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journal_title:European journal of oral sciences
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journal_title:European journal of oral sciences
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journal_title:European journal of oral sciences
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journal_title:European journal of oral sciences
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journal_title:European journal of oral sciences
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:European journal of oral sciences
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journal_title:European journal of oral sciences
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