Abstract:
:Polydopamine-templated hydroxyapatite (tHA) is a type of nano-biomaterial that can promote osteogenesis in bone tissue engineering. However, high concentrations of tHA stimulate production of reactive oxygen species (ROS), resulting in cell injury and apoptosis. Metformin has been demonstrated to activate the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway, which induces autophagy and decreases ROS production to prevent apoptosis. The present study was performed to investigate the potential application of tHA in combination with metformin in periodontal bone tissue engineering. Human periodontal ligament stem cells (hPDLSCs) were exposed to tHA in the presence or absence of metformin, and cytocompatibility and osteogenesis were detected by related assays. Additionally, the autophagy signaling pathway was analyzed by western blotting. Polydopamine-templated hydroxyapatite, in combination with metformin, substantially reduced ROS production and apoptosis, and enhanced proliferation and osteogenic differentiation of hPDLSCs. Enhanced levels of microtubule-associated protein 1 light chain 3 II and Beclin-1 were observed after exposure to tHA plus metformin. Expression of phosphorylated AMPK was increased and that of phosphorylated mammalian target of rapamycin (mTOR) was decreased after exposure to tHA plus metformin. Taken together, our results demonstrate that tHA, combined with metformin, increases the viability of hPDLSCs via the AMPK/mTOR signaling pathway by regulating autophagy and further improving the osteogenic effect.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Yang Z,Gao X,Zhou M,Kuang Y,Xiang M,Li J,Song Jdoi
10.1111/eos.12616subject
Has Abstractpub_date
2019-06-01 00:00:00pages
210-221issue
3eissn
0909-8836issn
1600-0722journal_volume
127pub_type
杂志文章abstract::This study explored the relationships between sex, socio-economic status, social support, social network, dental clinical status, dental pain, oral health-related quality of life (OHRQoL), and self-rated oral health (SROH) in adolescents. A cross-sectional study involving 542 adolescents, aged 12-14 yr, was conducted ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12605
更新日期:2019-04-01 00:00:00
abstract::The aim of this study was to assess post-trial treatment costs, clinical outcomes [decayed, missing or filled surfaces (DMFS) scores], and utilization of dental services among adolescents who had participated in a randomized clinical trial (RCT) in Pori, Finland, in 2001-2005. At baseline the children were 11-12 yr of...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2010.00726.x
更新日期:2010-06-01 00:00:00
abstract::The purpose of this study was to investigate the efficacy of an etchant in titanium bonding. The aqueous etchant consisted of 0, 5, or 10 wt% ammonium hydrogen fluoride (AHF) in combination with 0, 0.03, 0.3, or 3 wt% cupric chloride (CC). The two primers used were a phosphate-methacrylate primer and a thiophosphate-m...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.0909-8836.2004.00090.x
更新日期:2004-02-01 00:00:00
abstract::The salivary pellicle is a negatively charged protein film, to which oral bacteria readily adhere. Chitosans are cationic biomolecules with known antimicrobial properties that can be modified in different ways to enhance its antimicrobial activity. Here, we determined the changes in surface chemical composition using ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2007.00454.x
更新日期:2007-08-01 00:00:00
abstract::The non-stick properties of thin-film coatings on dental-restorative instruments were investigated by static contact-angle measurement using dental filler resin as well as by scanning electron microscopy of the amount of sticking dental restorative material. Furthermore, using a customized dipping measurement set-up, ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12372
更新日期:2017-12-01 00:00:00
abstract::Fast eating has been shown to increase the risk of overweight in both children and adults. The objectives of the present study were to investigate the correlation between chewing rate and the number of chews per mouthful and to evaluate if they were associated with the weight of meal intake. Thirty healthy subjects, a...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12583
更新日期:2019-02-01 00:00:00
abstract::Salivary tissue kallikrein is stored in an active form in human salivary glands. Pre-kallikrein has been demonstrated in mixed saliva, but it is not clear if the various salivary glands contribute equally. This study set out to determine if pre-kallikrein is present in human parotid and submandibular salivas at rest, ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2001.00091.x
更新日期:2001-10-01 00:00:00
abstract::Diagnostic delay in oropharyngeal cancer may be associated with poor prognosis. As controversy exists on this topic because of contradictory results, the aim of this study was to perform a systematic review of the relationship between total diagnostic delay and advanced disease stages. A systematic search of MEDLINE, ...
journal_title:European journal of oral sciences
pub_type: 杂志文章,meta分析,评审
doi:10.1111/j.1600-0722.2009.00672.x
更新日期:2009-10-01 00:00:00
abstract::We assessed the effect of certain oral health-related behaviours on adults' dental health. As part of the Finnish nationwide Health 2000 Survey, dentate subjects, 30-64 yr of age, reported their frequency of consumption of eight sugar- and xylitol-containing products, together with toothbrushing frequency and use of f...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2010.00804.x
更新日期:2011-02-01 00:00:00
abstract::RhoA plays a fundamental role in regulation of the actin cytoskeleton, intercellular attachment, and cell proliferation. During amelogenesis, ameloblasts (which produce the enamel proteins) undergo dramatic cytoskeletal changes and the RhoA protein level is up-regulated. Transgenic mice were generated that express a d...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12059
更新日期:2013-08-01 00:00:00
abstract::The objective of this clinical study was to assess the internal and marginal accuracy of computer-aided design/computer-aided manufacturing (CAD/CAM)-generated four-unit all-ceramic posterior fixed dental prostheses (FDPs). The data were compared with the results of three-unit metal-ceramic and all-ceramic FDPs that w...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2008.00580.x
更新日期:2008-12-01 00:00:00
abstract::Substantial evidence exists to suggest a role for T-cells in periodontal disease. As yet, however, the T-cell receptors remain to be characterised at the molecular level. The expression and the nucleotide sequence of genes from the T-cell receptor beta variable (TCRBV) gene families 2, 6 and 8 were analyzed in periodo...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1997.tb02136.x
更新日期:1997-10-01 00:00:00
abstract::There is evidence that the quantity of antigen load is crucial for the activation of IgA immune responses. In order to investigate the relevance of these findings in aggressive periodontitis, salivary antibody responses were measured during non-surgical and antibiotic treatment. Twenty-one patients with generalized ag...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2003.00040.x
更新日期:2003-06-01 00:00:00
abstract::The Oral Impacts on Daily Performance (OIDP) instrument was translated into Norwegian and reviewed for cultural and conceptual equivalence by a group of bilingual academics. A sample of employees from the University of Bergen completed the Norwegian OIDP frequency questionnaire twice. A total of 173 and 108 subjects p...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2005.00225.x
更新日期:2005-08-01 00:00:00
abstract::The outer membrane of Porphyromonas gingivalis, an oral strict anaerobe, was isolated by sucrose density gradient centrifugation. The outer membrane obtained by the differential detergent extraction method, previously reported, showed an essentially similar protein pattern on sodium dodecyl sulfate-polyacrylamide gel ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2002.11171.x
更新日期:2002-04-01 00:00:00
abstract::Relearning of motor skills is important in neurorehabilitation. We investigated the improvement of training success during simple tongue protrusion (two force levels) and a more complex tongue-training paradigm using the Tongue Drive System (TDS). We also compared subject-based reports of fun, pain, fatigue, and motiv...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2011.00894.x
更新日期:2012-02-01 00:00:00
abstract::Secretion rates and composition of unstimulated and chewing-stimulated whole saliva and 3% citric acid stimulated parotid and submandibular-sublingual secretions were studied in 12 hypertensive patients during withdrawal of and re-exposure to antihypertensive pharmacotherapy. All the patients' blood pressures were wel...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1996.tb00076.x
更新日期:1996-06-01 00:00:00
abstract::Ectopic tooth transplants are known to receive rich innervation of local neurons, but the precise location and structural features of neurites in the pulp and periodontal ligament (PDL) of such transplants are unclear. In this experiment, the molar tooth germs of rat embryos and incisors of young rats were subcutaneou...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2008.00546.x
更新日期:2008-08-01 00:00:00
abstract::In vivo and ex vivo gene transfer are being developed for localized skeletal regeneration. These strategies for tissue regeneration were tested in an adult ferret model of vital pulp therapy. In this model a reversible pulpitis was induced first. Then after 3 d, the pulps were directly infected with recombinant virus ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2001.00150.x
更新日期:2001-12-01 00:00:00
abstract::Clinical studies have shown conflicting results for the reduction of approximal caries when chlorhexidine gel has been applied either in trays or by flossing. To investigate whether these modes of treatment affect the colonization of mutans streptococci (MS) differently, we quantified MS in saliva and on various tooth...
journal_title:European journal of oral sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.0909-8836.1999.eos1070303.x
更新日期:1999-06-01 00:00:00
abstract::The objective of this study was to investigate differences in self-perceived and dentist-determined treatment need in Australian-born and migrant residents of Australia. Participants in the National Survey of Adult Oral Health 2004-06 were categorized into six groups according to country of birth. Interview and examin...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12373
更新日期:2017-12-01 00:00:00
abstract::The aims of this study were to evaluate the effect of two caries-preventive programs, and to apply the laser fluorescence method, DIAGNOdent, for longitudinal quantification of changes in incipient carious lesions. Twelve subjects with 127 test teeth exhibiting white spot lesions on the buccal surfaces after completed...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2006.00395.x
更新日期:2006-10-01 00:00:00
abstract::This study evaluated the effect of two desensitizers on inhibition of dentin demineralization, after immersion in artificial saliva using micro-computed tomography (μCT). Dentin blocks cut from bovine incisors were treated with deionized water (DW, a negative control) or one of three desensitizers: a fluoride varnish ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12155
更新日期:2014-12-01 00:00:00
abstract::Dental plaque fluid is normally supersaturated with respect to enamel mineral but this may change to a state of undersaturation when plaque pH falls following sugar exposure, placing the adjacent enamel at risk of caries. We have determined the saturation status of the fluid in both resting and fermenting plaque follo...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1046/j.0909-8836.1999.eos107404.x
更新日期:1999-08-01 00:00:00
abstract::Coadhesion between oral microbial pairs is an established factor in the spatiotemporal development and prevalence of mixed-species communities in early dental plaque in vivo. This study compares removal and de novo adhesion of pairs of coadhering and non-coadhering oral actinomyces and streptococci by sonic brushing o...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.0909-8836.2003.00078.x
更新日期:2003-12-01 00:00:00
abstract::Attention deficit hyperactivity disorder (ADHD) is a common developmental disorder. The present study tested the hypotheses that children with ADHD, particularly those exhibiting severe hyperactivity and impulsivity, have a different stress reaction (measured by salivary cortisol) during a dental recall visit and are ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2007.00423.x
更新日期:2007-02-01 00:00:00
abstract::Genetic factors underlie the susceptibility and the resistance to dental fluorosis (DF). The A/J (DF susceptible) and 129P3/J (DF resistant) mouse strains have previously been used to detect quantitative trait loci (QTLs) associated with DF on chromosome (Chr) 2 and Chr 11. In the present study, increased marker densi...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2011.00868.x
更新日期:2011-12-01 00:00:00
abstract::High mobility group protein B1 (HMGB1), a bone-active cytokine and an osteocyte alarmin, might have dual functions in bone metabolism that could benefit bone formation and accelerate osteoclastogenic activity. High mobility group protein B1 was recently shown to be involved in tooth movement. Here, we investigated the...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12644
更新日期:2019-10-01 00:00:00
abstract::Halitosis is most often caused by oral conditions. Volatile sulfur compounds (VSC), constituting the major components of oral malodor, are produced by anaerobic, gram-negative bacteria retained mainly in periodontal pockets or on the tongue dorsum. Sulfur-containing amino acids serve as substrate for these bacteria. V...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1997.tb00241.x
更新日期:1997-10-01 00:00:00
abstract::The aim of the present study was to examine to what extent pH affects human fibroblast repopulation of an experimental wound in vitro laying special emphasis on cell migration, DNA synthesis, and alterations in cell morphology. Wounds were simulated in vitro by partially scraping off monolayers of human embryonic dipl...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1995.tb00016.x
更新日期:1995-06-01 00:00:00