Abstract:
:Substantial evidence exists to suggest a role for T-cells in periodontal disease. As yet, however, the T-cell receptors remain to be characterised at the molecular level. The expression and the nucleotide sequence of genes from the T-cell receptor beta variable (TCRBV) gene families 2, 6 and 8 were analyzed in periodontal tissue from 24 patients with chronic adult periodontal disease (CAPD) and peripheral blood lymphocytes (PBL) of 16 of these patients. A restriction in the expression of these TCRBV gene families was detected in periodontal tissue from 14/24 patients with CAPD, and the pattern of gene expression was often different between individual patients; however there was no restriction in TCRBV gene expression in matched PBL samples from 8 of these 14 patients. Quantitative RT PCR analysis of samples from 5 CAPD patients who expressed all 3 TCRBV gene families in their periodontal tissues did not reveal any significant differences in the levels of gene expression in periodontal tissue and PBL. In contrast to the findings with some CAPD patients, genes from all 3 TCRBV families were always expressed in periodontal tissue and PBL from disease-free control subjects. PCR products from both the PBL and periodontal tissue of CAPD patients were cloned and sequenced; analysis of the nucleotide sequence revealed diversity with respect to the expression of TCRB joining (TCRBJ) and TCRB diversity (TCRBD) genes and the sequence of the junctional region in all samples analysed. In conclusion, in CAPD, the pattern of TCRBV gene expression in periodontal tissue is often but not always different from that in PBL and healthy periodontal tissue, which may indicate, in some cases, a local influence on particular T-cell subsets which is relevant to the pathogenesis of periodontal disease. However, the expressed TCRB genes are heterogeneous at the nucleotide level, emphasising the underlying complexity at the molecular level in the local T-cell response in CAPD.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Geatch DR,Ross DA,Heasman PA,Taylor JJdoi
10.1111/j.1600-0722.1997.tb02136.xsubject
Has Abstractpub_date
1997-10-01 00:00:00pages
397-404issue
5 Pt 1eissn
0909-8836issn
1600-0722journal_volume
105pub_type
杂志文章abstract::Xylitol reduces plaque but the reduction mechanism is largely unknown. The main aim of the present study was to determine whether the xylitol-induced reduction in the amount of plaque and the number of mutans streptococci could be demonstrated in subjects with (presumably) high levels of xylitol-resistant (XR; not inh...
journal_title:European journal of oral sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1600-0722.1997.tb00196.x
更新日期:1997-04-01 00:00:00
abstract::The salivary pellicle is a negatively charged protein film, to which oral bacteria readily adhere. Chitosans are cationic biomolecules with known antimicrobial properties that can be modified in different ways to enhance its antimicrobial activity. Here, we determined the changes in surface chemical composition using ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2007.00454.x
更新日期:2007-08-01 00:00:00
abstract::Outcomes of hypnotherapy (HT), group therapy (GT) and individual systematic desensitization (SD) on extreme dental anxiety in adults aged 19-65 yr were compared by regular attendance behaviors, changes in dental anxiety and changes in beliefs about dentists and treatment after 3 yr. Treatment groups were comparable wi...
journal_title:European journal of oral sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1034/j.1600-0722.2002.21234.x
更新日期:2002-08-01 00:00:00
abstract::As a result of their presence throughout the mouth in the submucosa or between muscle fibers, minor salivary glands secrete directly and continuously into the oral cavity, providing mucosal surfaces with highly glycosylated proteins that are active in bacterial aggregation and in oral tissue lubrication. In this study...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2009.00700.x
更新日期:2010-02-01 00:00:00
abstract::This study compared SYBR Green real-time quantitative PCR (qPCR) with standard plate counting for the enumeration of Streptococcus mutans in oral samples. Oral samples (n = 710) were collected from high-caries-risk children for quantification of S. mutans by qPCR using primer pairs. The S. mutans copy number was calcu...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2011.00888.x
更新日期:2011-12-01 00:00:00
abstract::It was the purpose of this article to analyze the (micro) morphological structure of enamel at different stages of development in order to deduce movement patterns of ameloblasts during formation of the human dental primordium. Developing enamel and overlying ameloblasts were dried and fractured for scanning electron ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2006.00314.x
更新日期:2006-05-01 00:00:00
abstract::The Enamel Defects Index (EDI) was created based on three innovative principles: (i) a basic level of the three major categories of defects; (ii) more detailed subcategories of each major category; and (iii) each category scored independently as present [1] or absent [0], simplifying decision making. The aim of this i...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2006.00294.x
更新日期:2006-05-01 00:00:00
abstract::The aim of the present study was to examine the accuracy of linear measurements on radiographs of interproximal bone loss in intrabony defects utilizing the gold standard of surgical measurements. In 22 patients with untreated advanced periodontal disease, 33 standardized radiographs were taken presurgically. The hori...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2000.00729.x
更新日期:2000-02-01 00:00:00
abstract::We examined the effect of warm air-drying on the enamel bond strengths and the surface free-energy of three single-step self-etch adhesives. Bovine mandibular incisors were mounted in self-curing resin and then wet ground with #600 silicon carbide (SiC) paper. The adhesives were applied according to the instructions o...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12061
更新日期:2013-08-01 00:00:00
abstract::Plaque fluoride concentrations ([F]) are directly related to plaque calcium concentrations [Ca]. Attempts to increase plaque F uptake from dentifrices or rinses have used methods designed to increase plaque [Ca] but with inconsistent results. This double-blind, double-crossover study tested the effect of a 150 mM calc...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2006.00409.x
更新日期:2006-12-01 00:00:00
abstract::Paraffin-stimulated saliva samples were collected from 140 children 3- and 4-yr old attending nine nursery schools in Latvia. The salivary levels of mutans streptococci were rated from zero to 3 after being cultured on a commercially available strip selective for these microorganisms. Of the children, 29.3% were rated...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1995.tb00171.x
更新日期:1995-08-01 00:00:00
abstract::The aim of the present investigation was to map the presence of prophylactic measures in organized Swedish dental health care systems, focusing on personnel working with children and adolescents. The study was conducted by sending a questionnaire to all public dental clinics in Sweden (830 clinics in all). The results...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1996.tb00115.x
更新日期:1996-08-01 00:00:00
abstract::The human host defense peptide, LL-37, is an important player in the first line of defense against invading microorganisms. LL-37 and its precursor, hCAP18, have been detected in unstimulated whole saliva but no reports showing hCAP18/LL-37 in isolated, parotid, and/or submandibular/sublingual saliva have been present...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12407
更新日期:2018-04-01 00:00:00
abstract::The objective of this study was twofold, namely to evaluate the effectiveness of a dental clinical practice guideline on the management of asymptomatic impacted lower third molars (i) on referral rates and (ii) on dentists' change in knowledge. A two-arm cluster randomized controlled trial, with pre- and post-test ass...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2005.00232.x
更新日期:2005-10-01 00:00:00
abstract::Aesthetic alternatives to stainless-steel crowns for restoring primary molars attain growing interest. We studied the mechanical properties of prefabricated zirconia crowns and conventional crowns. Three brands of prefabricated zirconia crowns were compared with computer-aided design/computer-aided manufacturing (CAD/...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12645
更新日期:2019-08-01 00:00:00
abstract::Recent studies have shown that bone morphogenetic protein 9 (BMP-9) can induce osteogenic differentiation in human periodontal stem cells and human periodontal ligament fibroblasts (PDLFs). Bone morphogenetic protein 9 may be used in periodontal tissue regeneration because of its potent osteoinductive ability. Human p...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12336
更新日期:2017-04-01 00:00:00
abstract::Osteoclasts are hematopoietic in origin and formed by proliferation, differentiation and fusion of osteoclast progenitor cells. However, the signal transducing mechanisms involved in generation of osteoclasts are not clear. We have used two well-known adenylate cyclase stimulators to examine the effect of cyclic AMP (...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1046/j.0909-8836.1999.eos107108.x
更新日期:1999-02-01 00:00:00
abstract::Dentinogenesis imperfecta type II and dentin dysplasia type II are diseases resulting in abnormal dentin formation, which have been mapped to overlapping regions of human chromosome 4q defined by markers D4S2691 and D4S2692 (6.6 cM) and D4S3291 and SPP1 (14.1 cM), respectively. Recently, two of the major non-collageno...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1998.tb02180.x
更新日期:1998-01-01 00:00:00
abstract::The aims were to test the hypotheses that experimental masseter muscle pain leads to recruitment and/or derecruitment of motor units at different sites within the masseter and that the patterns of change in motor unit activity differ between sites. Single motor unit (SMU) activity was recorded at two sites within the ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12561
更新日期:2018-10-01 00:00:00
abstract::The purpose of this study was to evaluate the durability of one-bottle self-etching adhesive during long-term water-storage testing. Resin-dentin bonded specimens were prepared using four commercially available one-bottle self-etching adhesives. The specimens were sectioned perpendicular to the adhesive interface to p...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2009.00664.x
更新日期:2009-10-01 00:00:00
abstract::The aim of this study was to explore the prevalence and possible explanatory factors of dental avoidance due to dental anxiety among 12 to 18 year olds treated within the Norwegian Public Dental Service. A total of 754 20-yr-olds completed a questionnaire and three psychometric scales, the Dental Fear Scale (DFS), the...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1046/j.0909-8836.1999.eos107602.x
更新日期:1999-12-01 00:00:00
abstract::To investigate the arrangement of biofilms formed in vivo, volunteers wore splints with slabs of six different dental materials inserted to collect smooth surface plaque. After 5 d of undisturbed plaque accumulation, the specimens were vital stained and analyzed by the confocal laser scanning microscopy (CLSM) to eval...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1046/j.0909-8836.2001.101160.x
更新日期:2002-02-01 00:00:00
abstract::The Runx2 gene is a master transcription factor of bone and plays a role in all stages of bone formation. It is essential for the initial commitment of mesenchymal cells to the osteoblastic lineage and also controls the proliferation, differentiation, and maintenance of these cells. Control is complex, with involvemen...
journal_title:European journal of oral sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0722.2006.00399.x
更新日期:2006-10-01 00:00:00
abstract::This study investigated the importance of dental beliefs and the predictive value of the Dental Belief Survey (DBS) in dental-fear treatment. The sample comprised 117 adult patients seeking treatment at a dental-fear clinic. Pretreatment data were collected during a screening procedure, including two visits to the den...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2003.00016.x
更新日期:2003-04-01 00:00:00
abstract::The aim of this study was to assess post-trial treatment costs, clinical outcomes [decayed, missing or filled surfaces (DMFS) scores], and utilization of dental services among adolescents who had participated in a randomized clinical trial (RCT) in Pori, Finland, in 2001-2005. At baseline the children were 11-12 yr of...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2010.00726.x
更新日期:2010-06-01 00:00:00
abstract::Native and recombinant human bone morphogenetic/osteogenic proteins (BMPs/ OPs) singly initiate bone induction in vivo. The finding of synchronous but spatially different BMPs/OPs expression during periodontal tissue morphogenesis suggests novel therapeutic approaches using morphogen combinations based on recapitulati...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2001.00041.x
更新日期:2001-08-01 00:00:00
abstract::Mandibular movements occur through the triggering of trigeminal motoneurons. Aberrant movements by orofacial muscles are characteristic of orofacial motor disorders, such as nocturnal bruxism (clenching or grinding of the dentition during sleep). Previous studies have suggested that autonomic changes occur during brux...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2009.00686.x
更新日期:2009-12-01 00:00:00
abstract::The aim of this study was to validate the use of digitized periapical radiographs in evaluating orthodontically induced apical root resorption against micro-computed tomography (micro-CT) scanning as a criterion standard test. In a standardized experimental protocol, 29 premolars in 16 subjects were tipped buccally fo...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2008.00559.x
更新日期:2008-10-01 00:00:00
abstract::There is evidence that the quantity of antigen load is crucial for the activation of IgA immune responses. In order to investigate the relevance of these findings in aggressive periodontitis, salivary antibody responses were measured during non-surgical and antibiotic treatment. Twenty-one patients with generalized ag...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2003.00040.x
更新日期:2003-06-01 00:00:00
abstract::The expression of HLA Class II antigens on the surfaces of immunocompetent cells and the presence of CD1a+ cells (Langerhans cells) are important components of antigen presentation. Quantitative variations in HLA class II expression on antigen-presenting cells play a role in immune regulation. An indirect immunofluore...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1995.tb00161.x
更新日期:1995-08-01 00:00:00