Abstract：
:This study compared SYBR Green real-time quantitative PCR (qPCR) with standard plate counting for the enumeration of Streptococcus mutans in oral samples. Oral samples (n = 710) were collected from high-caries-risk children for quantification of S. mutans by qPCR using primer pairs. The S. mutans copy number was calculated with reference to a qPCR quantification cycle (Cq) standard curve and compared with the absorbance value at 600 nm of a standard suspension of S. mutans UA159. The S. mutans copy number results were evaluated in relation to standard plate count (SPC) results obtained from each sample following culture on Petri plates containing S. mutans selective media and reported as colony-forming units (CFUs). The mean S. mutans copy number calculated from qPCR was higher than the SPC CFUs (1.3 × 10(6) and 1.5 × 10(5) CFUs, respectively). The qPCR values were usually higher in individual samples and qPCR detected the presence of S. mutans 84% (231/276) of the time that the SPC did not, compared with 33% (4/12) of the time when qPCR failed to detect S. mutans and the SPC did. The qPCR technique was found to be more sensitive for detection of S. mutans from oral samples, a method that is not dependent on the viability of the sample taken and therefore is proposed as a more reliable and efficient means of quantification of S. mutans.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Childers NK,Osgood RC,Hsu KL,Manmontri C,Momeni SS,Mahtani HK,Cutter GR,Ruby JDdoi
10.1111/j.1600-0722.2011.00888.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
447-54issue
6eissn
0909-8836issn
1600-0722journal_volume
119pub_type
杂志文章abstract：:The aim of this study was to characterize the sarcoplasmic-endoplasmic reticulum Ca-ATPase (SERCA) isoforms in rabbit masticatory muscles compared with those in fast-twitch muscle. It was hypothesized that combined expression of the SERCA isoforms in fast- and slow-twitch muscles accounts for lower Ca-ATPase activity....
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12098
更新日期：2014-02-01 00:00:00
abstract：:Low concentrations of fluoride have a beneficial effect on enamel and dentin de- and remineralization. After fluoride treatments, such as topical applications, rinses or dentifrices, salivary fluoride concentrations decrease exponentially in a biphasic manner to very low concentrations within a few hours. For treatmen...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0722.1997.tb00231.x
更新日期：1997-10-01 00:00:00
abstract：:The objective of this study was to investigate differences in self-perceived and dentist-determined treatment need in Australian-born and migrant residents of Australia. Participants in the National Survey of Adult Oral Health 2004-06 were categorized into six groups according to country of birth. Interview and examin...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12373
更新日期：2017-12-01 00:00:00
abstract：:This study measured the polymerization contraction of a resin composite cured at three levels of energy density, each attained at six different levels of power density. The polymerization contraction of the composite was recorded by the method of the deflecting disc (n = 5) for 1 h following the start of irradiation. ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2005.00239.x
更新日期：2005-10-01 00:00:00
abstract：:Dental personnel and orthopedic surgeons are at risk when manually handling products containing methyl methacrylate (MMA). Dental products may also contain cross-linking agents such as ethylene glycol dimethacrylate (EGDMA) or 1,4-butanediol dimethacrylate (1,4-BDMA). Skin contact with monomers can cause hand eczema, ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2003.00064.x
更新日期：2003-10-01 00:00:00
abstract：:The skeletal remains of Giovanna of Austria (1547-1578), daughter of the Emperor Ferdinand I of Habsburg (1503-1564) and first wife of the Grand Duke of Tuscany, Francesco I (1541-1587), exhumed from the Basilica of San Lorenzo in Florence, were submitted to paleopathological study. Examination of the dentition, which...
journal_title：European journal of oral sciences
pub_type： 传,历史文章,杂志文章
doi：10.1111/eos.12097
更新日期：2014-02-01 00:00:00
abstract：:Formation of crystals in the enamel space releases protons that need to be buffered to sustain mineral accretion. We hypothesized that apical cystic fibrosis transmembrane conductance regulator (CFTR) in maturation ameloblasts transduces chloride into forming enamel as a critical step to secrete bicarbonates. We teste...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12163
更新日期：2015-02-01 00:00:00
abstract：:This study investigated the effect of repeated tongue motor tasks on suprahyoid muscle activity and tongue pressure. Fourteen participants performed three series of a standardized tongue-lift training (TLT) task on each of five consecutive days. Electromyographic (EMG) activity from suprahyoid muscles and tongue press...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12300
更新日期：2016-12-01 00:00:00
abstract：:Motivation plays an important role in the outcome of motor learning but has not received attention in tongue-training-induced plasticity of the corticomotor pathways. The present study investigated the influence of two different motivational conditions and gender on performance during a complex tongue-training paradig...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12022
更新日期：2013-04-01 00:00:00
abstract：:Many factors have been proposed to influence oral infection with yeast. The aim of this study was to determine the prevalence of oral yeasts in oral lichen planus (OLP) patients and control subjects, and to perform a multiple logistic regression analysis to identify factors that influence oral Candida infection in OLP...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12073
更新日期：2013-10-01 00:00:00
abstract：:Genetic factors underlie the susceptibility and the resistance to dental fluorosis (DF). The A/J (DF susceptible) and 129P3/J (DF resistant) mouse strains have previously been used to detect quantitative trait loci (QTLs) associated with DF on chromosome (Chr) 2 and Chr 11. In the present study, increased marker densi...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00868.x
更新日期：2011-12-01 00:00:00
abstract：:The aim of the present study was to examine to what extent pH affects human fibroblast repopulation of an experimental wound in vitro laying special emphasis on cell migration, DNA synthesis, and alterations in cell morphology. Wounds were simulated in vitro by partially scraping off monolayers of human embryonic dipl...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1995.tb00016.x
更新日期：1995-06-01 00:00:00
abstract：:The physiological variations of fibrinolytic activity (FA) in saliva were investigated in a population of 149 healthy volunteers including 12 pregnant women. An increase in FA could be observed with increasing age. Marked variations were observed during daytime with a peak value early in the morning. Higher FA values ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00089.x
更新日期：1996-08-01 00:00:00
abstract：:Periodontal diseases, which are characterized by destruction of the connective tissues responsible for restraining the teeth within the jaw, are the main cause of tooth loss. Periodontal regeneration mediated by human periodontal ligament stem cells (hPDLSCs) may offer an alternative strategy for the treatment of peri...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2012.00975.x
更新日期：2012-08-01 00:00:00
abstract：:The objective of this study was twofold, namely to evaluate the effectiveness of a dental clinical practice guideline on the management of asymptomatic impacted lower third molars (i) on referral rates and (ii) on dentists' change in knowledge. A two-arm cluster randomized controlled trial, with pre- and post-test ass...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2005.00232.x
更新日期：2005-10-01 00:00:00
abstract：:While Er:YAG laser systems are in extensive use for caries removal and cavity preparation, the effects of such treatment on pulp tissue remain unclear. This study evaluates these systems using immunohistochemical methods and compares the results with information gained from treatment using conventional burs. Cervical ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2002.21282.x
更新日期：2002-06-01 00:00:00
abstract：:The demineralization of dentin was studied when placed adjacent to one of four experimental denture base-resins. These experimental resins contained polymethylmethacrylate (PMMA) and 0, 5, 10, 20 or 30 wt% surface reaction-type prereacted glass-ionomer (S-PRG) filler, respectively. A dentin thin-section was sandwiched...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2009.00678.x
更新日期：2009-12-01 00:00:00
abstract：:The aim of the present study was to determine the nature of the cells associated with the formation of reparative acellular extrinsic fiber cementum (AEFC). Calcifying collagen membranes, hydroxyapatite particles and/or non-resorbable ePTFE membranes were implanted in lesions made in the periodontium of the rat mandib...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1998.tb02200.x
更新日期：1998-01-01 00:00:00
abstract：:RhoA plays a fundamental role in regulation of the actin cytoskeleton, intercellular attachment, and cell proliferation. During amelogenesis, ameloblasts (which produce the enamel proteins) undergo dramatic cytoskeletal changes and the RhoA protein level is up-regulated. Transgenic mice were generated that express a d...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12059
更新日期：2013-08-01 00:00:00
abstract：:The aim of this study was to explore the prevalence and possible explanatory factors of dental avoidance due to dental anxiety among 12 to 18 year olds treated within the Norwegian Public Dental Service. A total of 754 20-yr-olds completed a questionnaire and three psychometric scales, the Dental Fear Scale (DFS), the...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1999.eos107602.x
更新日期：1999-12-01 00:00:00
abstract：:The aim of this study was to investigate the relationship between dental fluorosis and dental caries among western Saharan refugee children. The western Saharan child population is characterized by adverse living conditions, an unbalanced diet, poor oral hygiene habits, and a concentration of fluoride in the drinking ...
journal_title：European journal of oral sciences
pub_type： 杂志文章,多中心研究
doi：10.1111/j.1600-0722.2008.00583.x
更新日期：2008-12-01 00:00:00
abstract：:The aim of this study was to validate the use of digitized periapical radiographs in evaluating orthodontically induced apical root resorption against micro-computed tomography (micro-CT) scanning as a criterion standard test. In a standardized experimental protocol, 29 premolars in 16 subjects were tipped buccally fo...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2008.00559.x
更新日期：2008-10-01 00:00:00
abstract：:The purpose was to investigate if antigenic fragments of aggrecan and cartilage oligomeric matrix protein (COMP) are detectable by enzyme-linked immunosorbent assay in lavage fluids from the temporomandibular joint (TMJ) and to examine if the relative content of these cartilage markers changes during development of os...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00254.x
更新日期：1997-08-01 00:00:00
abstract：:Treatment programs to prevent perinatal and postnatal HIV-1 transmission have become available in sub-Saharan Africa, leading to an emerging population of HIV-1 exposed uninfected (HEU) children. Exposure to HIV-1 in utero and during breastfeeding may increase the risk of morbidity and mortality in HEU children. This ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12669
更新日期：2020-02-01 00:00:00
abstract：:High mobility group protein B1 (HMGB1), a bone-active cytokine and an osteocyte alarmin, might have dual functions in bone metabolism that could benefit bone formation and accelerate osteoclastogenic activity. High mobility group protein B1 was recently shown to be involved in tooth movement. Here, we investigated the...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12644
更新日期：2019-10-01 00:00:00
abstract：:The aim of this in vitro study was to investigate the relationship between bacterial infections in carious dentin when detected by two different methods -- polymerase chain reaction (PCR), and a laser fluorescence device, DIAGNOdent. Dentin was removed every 300 micro m in the direction of the pulp chamber in 10 extra...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2004.00161.x
更新日期：2004-10-01 00:00:00
abstract：:The purpose of the present study was to investigate the effect of an experimental primer containing a microperoxidase (MP-11) with 2-hydroxyethyl methacrylate on adhesive bonding of three different luting systems and dentin. The luting systems prepared were three etch-and-rinse systems (10-3/Super-Bond, 10-0/Super-Bon...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2009.00634.x
更新日期：2009-06-01 00:00:00
abstract：:The effect of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on bone healing was examined in calvarial defects in rabbits. Bicortical circular (critical size) defects were prepared in the calvarial bone of 16 rabbits. The defects were closed on the dural side and covered externally with expanded polyt...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00181.x
更新日期：1997-02-01 00:00:00
abstract：:This study evaluated the effect of topical fluoride application on enamel hardness after in-office bleaching. Twelve human incisors were cut along the long axis, resulting in 24 halves used in four treatment groups (n = 6 in each group): (i) untreated group (C); (ii) in-office bleaching material (B); (iii) treatment w...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12386
更新日期：2017-12-01 00:00:00
abstract：:Mandibular movements occur through the triggering of trigeminal motoneurons. Aberrant movements by orofacial muscles are characteristic of orofacial motor disorders, such as nocturnal bruxism (clenching or grinding of the dentition during sleep). Previous studies have suggested that autonomic changes occur during brux...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2009.00686.x
更新日期：2009-12-01 00:00:00
