Abstract:
:The aim of this study was to characterize the sarcoplasmic-endoplasmic reticulum Ca-ATPase (SERCA) isoforms in rabbit masticatory muscles compared with those in fast-twitch muscle. It was hypothesized that combined expression of the SERCA isoforms in fast- and slow-twitch muscles accounts for lower Ca-ATPase activity. SERCA was isolated by differential centrifugation, the isoforms were determined by ELISA, and the activity of each isoform was measured using a colorimetric method. Activity was tested for significance by anova, and the distribution of isoforms was assessed using the chi-square test (P < 0.05) and correlated to SERCA activity using Spearman's rank correlation. SERCA1 was predominant (90.5%) in fast-twitch muscle, whereas a mixture of SERCA isoforms was found in masticatory muscles: 62-78% was SERCA2, 20-37% was SERCA1, and the SERCA3 content was negligible. Depressor muscles showed a significantly higher content (77.8%) of SERCA2, and elevator muscles showed a higher content (35.4%) of SERCA1. Elevator muscles showed higher expression of SERCA2a (58%), and depressor muscles showed higher expression of SERCA2b (20%). The SERCA1 content was mainly SERCA1a and significantly higher for elevator muscles (33%), whereas depressor muscles showed a higher content of SERCA1b (4%). The SERCA1 content of fast-twitch muscle was mainly SERCA1a (88.5%). It is concluded that the mixture of different SERCA isoforms, along with a substantial content of SERCA2b, in masticatory muscles would support lower Ca-ATPase activity and calcium transport.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Sánchez GA,Trinks PW,Richard SB,Di Croce DE,Takara Ddoi
10.1111/eos.12098subject
Has Abstractpub_date
2014-02-01 00:00:00pages
36-41issue
1eissn
0909-8836issn
1600-0722journal_volume
122pub_type
杂志文章abstract::This cross-cultural study investigated adult dental fear patients in three countries. A joint intake interview questionnaire and a dental anxiety scale explored the level, background and concomitant factors of dental anxiety among patients at the Universities of Tel Aviv (Israel), Goteborg (Sweden), and Pittsburgh (US...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2000.108004268.x
更新日期:2000-08-01 00:00:00
abstract::To investigate the arrangement of biofilms formed in vivo, volunteers wore splints with slabs of six different dental materials inserted to collect smooth surface plaque. After 5 d of undisturbed plaque accumulation, the specimens were vital stained and analyzed by the confocal laser scanning microscopy (CLSM) to eval...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1046/j.0909-8836.2001.101160.x
更新日期:2002-02-01 00:00:00
abstract::The aim of our study was to compare the changes in children's oral health-related behavior, knowledge, and attitudes obtained using an oral health-promotion approach, a risk-strategy and promotion approach, and reference area, and to report changes in the behavior of children between the experimental and the control g...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2009.00640.x
更新日期:2009-08-01 00:00:00
abstract::Tooth development is regulated through a series of reciprocal interactions between the dental epithelium and mesenchyme and requires protein products of a number of genes. It has been reported that selective tooth agenesis is associated with mutations in human MSX and PAX9 genes. Mutational analysis of the two genes w...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2003.00036.x
更新日期:2003-06-01 00:00:00
abstract::This study evaluated the effect of two desensitizers on inhibition of dentin demineralization, after immersion in artificial saliva using micro-computed tomography (μCT). Dentin blocks cut from bovine incisors were treated with deionized water (DW, a negative control) or one of three desensitizers: a fluoride varnish ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12155
更新日期:2014-12-01 00:00:00
abstract::The goals of this study were to investigate whether (i) muscle activities are affected by different feedback strategies, (ii) the balancing behavior of the neuromuscular system is influenced by different force levels, and (iii) axial loading of the posterior teeth is a realistic biomechanical conception. In 10 healthy...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2008.00537.x
更新日期:2008-06-01 00:00:00
abstract::Relaxin (Rln) is an ovarian hormone that stimulates osteoclastic and osteoblastic activities and connective tissue turnover. To investigate the expression of Rln during orthodontic tooth movement, rats were implanted with orthodontic appliances that connected a spring from the upper incisors to the first molar with a ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2011.00814.x
更新日期:2011-04-01 00:00:00
abstract::Fluid and ion secretion from the submandibular gland (SMG) is mainly regulated by parasympathetic nerves. This study evaluated the effect of parasympathectomy on salivary secretion from normal and irradiated rat SMGs from 1 to 24 wk after denervation. Although stimulated salivary secretion was significantly lower in d...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12330
更新日期:2017-04-01 00:00:00
abstract::Ozone gas and photodynamic therapy (PDT) have been claimed to be antimicrobially effective. This study assessed their antimicrobial potential in vitro. Mature six-species oral biofilms were treated as follows (n = 9 per group): (i) a 60-s application of gasiform vacuum-ozone or vacuum alone (on wet or air-dried biofil...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2007.00418.x
更新日期:2007-02-01 00:00:00
abstract::Recent findings of simultaneous and well coordinated head-neck movements during single as well as rhythmic jaw opening-closing tasks has led to the conclusion that 'functional jaw movements' are the result of activation of jaw as well as neck muscles, leading to simultaneous movements in the temporomandibular, atlanto...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.0909-8836.2004.00098.x
更新日期:2004-02-01 00:00:00
abstract::Dental plaque fluid is normally supersaturated with respect to enamel mineral but this may change to a state of undersaturation when plaque pH falls following sugar exposure, placing the adjacent enamel at risk of caries. We have determined the saturation status of the fluid in both resting and fermenting plaque follo...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1046/j.0909-8836.1999.eos107404.x
更新日期:1999-08-01 00:00:00
abstract::The purpose of the present study was to investigate the effect of an experimental primer containing a microperoxidase (MP-11) with 2-hydroxyethyl methacrylate on adhesive bonding of three different luting systems and dentin. The luting systems prepared were three etch-and-rinse systems (10-3/Super-Bond, 10-0/Super-Bon...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2009.00634.x
更新日期:2009-06-01 00:00:00
abstract::We aimed to evaluate (i) changes in dental fear over time during pregnancy and after delivery among mothers and fathers and (ii) whether these changes inter-relate to changes in depression and anxiety. Longitudinal pilot data for the FinnBrain Cohort study were used. Of 254 pregnant families, 80% agreed to participate...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12026
更新日期:2013-06-01 00:00:00
abstract::It has been suggested that occlusal interferences may lead to pain and tenderness of the masticatory muscles. Tender jaw muscles are more sensitive to pressure pain, as assessed by means of pressure algometry. We tested the effects of occlusal interferences on the pressure pain threshold of the jaw muscles by means of...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2006.00298.x
更新日期:2006-04-01 00:00:00
abstract::Polydopamine-templated hydroxyapatite (tHA) is a type of nano-biomaterial that can promote osteogenesis in bone tissue engineering. However, high concentrations of tHA stimulate production of reactive oxygen species (ROS), resulting in cell injury and apoptosis. Metformin has been demonstrated to activate the adenosin...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12616
更新日期:2019-06-01 00:00:00
abstract::Kallikrein-related peptidase 4 (KLK4) is critical for proper dental enamel formation. Klk4 null mice, and humans with two defective KLK4 alleles have obvious enamel defects, with no other apparent phenotype. KLK4 mRNA or protein is reported to be present in tissues besides teeth, including prostate, ovary, kidney, liv...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2011.00834.x
更新日期:2011-12-01 00:00:00
abstract::It was the purpose of this article to analyze the (micro) morphological structure of enamel at different stages of development in order to deduce movement patterns of ameloblasts during formation of the human dental primordium. Developing enamel and overlying ameloblasts were dried and fractured for scanning electron ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2006.00314.x
更新日期:2006-05-01 00:00:00
abstract::Periodontal diseases, which are characterized by destruction of the connective tissues responsible for restraining the teeth within the jaw, are the main cause of tooth loss. Periodontal regeneration mediated by human periodontal ligament stem cells (hPDLSCs) may offer an alternative strategy for the treatment of peri...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2012.00975.x
更新日期:2012-08-01 00:00:00
abstract::The conditions under which mechanical properties of dentine are tested influence the values recorded. The aims of this study were to examine the effect of hydration on the mechanical properties of primary carious dentine and to provide information on changes in hardness and modulus of elasticity change caused by the d...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2004.00123.x
更新日期:2004-06-01 00:00:00
abstract::The objective of this study was to obtain a geographic world map of scientific production in dentistry by analysing published papers. Articles and reviews in the Dentistry, Oral Surgery & Medicine category published from 1999 to 2003 were accessed through the ISI database. The data were analyzed quantitatively (number...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2006.00244.x
更新日期:2006-04-01 00:00:00
abstract::The effect of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on bone healing was examined in calvarial defects in rabbits. Bicortical circular (critical size) defects were prepared in the calvarial bone of 16 rabbits. The defects were closed on the dural side and covered externally with expanded polyt...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1997.tb00181.x
更新日期:1997-02-01 00:00:00
abstract::The objective of this study was to investigate differences in self-perceived and dentist-determined treatment need in Australian-born and migrant residents of Australia. Participants in the National Survey of Adult Oral Health 2004-06 were categorized into six groups according to country of birth. Interview and examin...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12373
更新日期:2017-12-01 00:00:00
abstract::The principles of formation, renewal, and eruption of teeth are discussed. Numerous genetic aberrations may affect the formation and eruption of teeth. Cleidocranial dysplasia (CCD), caused by mutations in the runt-related transcription factor 2 (RUNX2) gene, is such a condition. The dental phenotype includes problems...
journal_title:European journal of oral sciences
pub_type: 杂志文章,评审
doi:10.1111/eos.12418
更新日期:2018-10-01 00:00:00
abstract::Root resorption appears to be an inevitable sequel to orthodontic tooth movement, occurring either apically or along surfaces in and around the pressure zones. However, there is uncertainty as to the location of initial cementum repair in resorbed areas as well as the time of onset of repair. The aim of the present st...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1995.tb00029.x
更新日期:1995-10-01 00:00:00
abstract::Immunoexpression of the extracellular matrix (ECM) proteins laminin, fibronectin, tenascin and types I, III and IV collagen was analyzed in the major and minor salivary glands of seven human fetuses at different gestational ages. The results showed the presence and localization of laminin, collagen IV and fibronectin ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2004.00169.x
更新日期:2004-12-01 00:00:00
abstract::This study evaluated the effect of topical fluoride application on enamel hardness after in-office bleaching. Twelve human incisors were cut along the long axis, resulting in 24 halves used in four treatment groups (n = 6 in each group): (i) untreated group (C); (ii) in-office bleaching material (B); (iii) treatment w...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12386
更新日期:2017-12-01 00:00:00
abstract::The aim of this study was to determine the effect of interleukin-10 (IL-10) on the gene expression of osteoclastogenic regulatory molecules in rat dental follicle cells. Interleukin-10 is an anti-inflammatory cytokine that inhibits alveolar bone resorption, but the molecular basis for this is unknown. Alveolar bone re...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2006.00283.x
更新日期:2006-02-01 00:00:00
abstract::Amelogenesis imperfecta is a group of hereditary enamel defects. Of the autosomal dominant forms, only the local hypoplastic type has been mapped to human chromosome 4q 13-4q21. Enamelin is a large enamel matrix protein secreted by ameloblasts. The purpose of this study was to determine the human chromosomal localizat...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2000.108005353.x
更新日期:2000-10-01 00:00:00
abstract::Inositol polyphosphate 4-phosphatase type II (INPP4B) is a phosphoinositide phosphatase that plays complex roles in the pathogenesis of different tumors. We aimed to explore the expression, clinicopathological significance, and prognostic value of INPP4B in oral squamous cell carcinoma (OSCC). Tissue microarrays that ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12673
更新日期:2020-02-01 00:00:00
abstract::The aim of this study was to explore the prevalence and possible explanatory factors of dental avoidance due to dental anxiety among 12 to 18 year olds treated within the Norwegian Public Dental Service. A total of 754 20-yr-olds completed a questionnaire and three psychometric scales, the Dental Fear Scale (DFS), the...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1046/j.0909-8836.1999.eos107602.x
更新日期:1999-12-01 00:00:00