Abstract：
:The aim of this study was to determine the effect of interleukin-10 (IL-10) on the gene expression of osteoclastogenic regulatory molecules in rat dental follicle cells. Interleukin-10 is an anti-inflammatory cytokine that inhibits alveolar bone resorption, but the molecular basis for this is unknown. Alveolar bone resorption is required for tooth eruption and the dental follicle functions to regulate the osteoclastogenesis needed for eruption. It does this by regulating its expression of receptor activator of nuclear factor-kappa B ligand (RANKL), colony-stimulating factor-1 (CSF-1), and osteoprotegerin (OPG). In this study, dental follicle cells were treated with IL-10, and the effect on gene expression of CSF-1, RANKL, and OPG was measured by reverse transcription-polymerase chain reaction (RT-PCR). Interleukin-10 enhanced the expression of OPG and down-regulated the expression of RANKL and CSF-1. Laser capture microdissection was carried out to detect IL-10 gene expression in the dental follicle. Knockdown of the IL-10 gene expression in the follicle cells was accomplished using a short interfering RNA (siRNA) targeting IL-10 mRNA. In these knockdowns, RANKL expression was increased and OPG expression was decreased. All of these results suggest that IL-10 inhibits bone resorption by up-regulating OPG expression while down-regulating expression of RANKL and CSF-1.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Liu D,Yao S,Wise GEdoi
10.1111/j.1600-0722.2006.00283.xsubject
Has Abstractpub_date
2006-02-01 00:00:00pages
42-9issue
1eissn
0909-8836issn
1600-0722pii
EOS283journal_volume
114pub_type
杂志文章abstract：:Relaxin (Rln) is an ovarian hormone that stimulates osteoclastic and osteoblastic activities and connective tissue turnover. To investigate the expression of Rln during orthodontic tooth movement, rats were implanted with orthodontic appliances that connected a spring from the upper incisors to the first molar with a ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00814.x
更新日期：2011-04-01 00:00:00
abstract：:Enamelysin is a matrix metalloproteinase (MMP-20) secreted by ameloblasts, previously shown to hydrolyze recombinant amelogenin. The purpose of this study was to use recombinant MMP-20 to further investigate the specific hydrolysis of peptide fragments containing cleavage sites for tyrosine-rich amelogenin peptide (TR...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1999.eos107506.x
更新日期：1999-10-01 00:00:00
abstract：:The outer membrane of Porphyromonas gingivalis, an oral strict anaerobe, was isolated by sucrose density gradient centrifugation. The outer membrane obtained by the differential detergent extraction method, previously reported, showed an essentially similar protein pattern on sodium dodecyl sulfate-polyacrylamide gel ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2002.11171.x
更新日期：2002-04-01 00:00:00
abstract：:Axonal regeneration of the inferior alveolar nerve (IAN) is a therapeutic target for functional recovery after peripheral nerve injury. Rifampicin exerts anti-apoptotic, anti-inflammatory, and anti-oxidant effects on nerve tissues that may enhance functional recovery after peripheral nerve injury. The aim of the prese...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12691
更新日期：2020-06-01 00:00:00
abstract：:The principles of formation, renewal, and eruption of teeth are discussed. Numerous genetic aberrations may affect the formation and eruption of teeth. Cleidocranial dysplasia (CCD), caused by mutations in the runt-related transcription factor 2 (RUNX2) gene, is such a condition. The dental phenotype includes problems...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/eos.12418
更新日期：2018-10-01 00:00:00
abstract：:The purposes of this survey were to describe dental health in a group of alcoholics and to analyze the influence of social background, dental health behavior and alcohol-related variables on dental health among alcoholics. The dental health in a group of consecutively admitted alcoholics (n = 195) was described with r...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00099.x
更新日期：1996-08-01 00:00:00
abstract：:Originally identified as the active components within osteoinductive extracts derived from bone, the BMPs now are known to include a large family of proteins within the TGF-beta superfamily of growth and differentiation factors. Members of the BMP family have been determined to be key signaling molecules in embryogene...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0722.1998.tb02170.x
更新日期：1998-01-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of combining fluoride dentifrice (FD) and varnish (FV) on in vitro enamel surface rehardening and on fluoride uptake under a pH-cycling regimen. Seventy-eight bovine enamel blocks with early lesions were used and 52 were divided into four treatment groups: (a) place...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2003.00007.x
更新日期：2003-02-01 00:00:00
abstract：:Granulocyte elastase was determined in the gingival crevicular fluid (GCF) of 18 periodontitis patients. They initially had similar severity of disease but had responded differently to 5-yr maintenance, 13 responders and 5 non-responders. A total of 102 sites were investigated and categorized as: i) consistently healt...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1995.tb00121.x
更新日期：1995-04-01 00:00:00
abstract：:The Wnt signalling pathway is involved in the development of oral cancer. When inactivated, secreted frizzled receptor protein 1 (SFRP1), a cellular Wnt-inhibitor protein, may enhance cancer development. The aim of this study was to assess the expression of SFRP1 in the invasive front of tongue squamous cell carcinoma...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12252
更新日期：2016-04-01 00:00:00
abstract：:Plasma arc light units for curing resin composites have been introduced with the claim of relatively short curing times. The purpose of the present study was to measure and compare elution of monomers from an experimental BisGMA-TEGDMA resin and a commercial resin composite when cured with a halogen unit and when cure...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108004341.x
更新日期：2000-08-01 00:00:00
abstract：:An oxygen inhibition layer develops on surfaces exposed to air during polymerization of particulate filling composite. This study assessed the thickness of the oxygen inhibition layer of short-fiber-reinforced composite in comparison with conventional particulate filling composites. The effect of an oxygen inhibition ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12167
更新日期：2015-02-01 00:00:00
abstract：:Fluoride is associated with a decrease in the incidence of dental caries, but excessive fluoride intake during tooth enamel formation can result in enamel fluorosis. Fluorosed enamel has increased porosity, which has been related to a delay in the removal of amelogenin proteins as the enamel matures. This delay in pro...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2006.00303.x
更新日期：2006-05-01 00:00:00
abstract：:Ectopic tooth transplants are known to receive rich innervation of local neurons, but the precise location and structural features of neurites in the pulp and periodontal ligament (PDL) of such transplants are unclear. In this experiment, the molar tooth germs of rat embryos and incisors of young rats were subcutaneou...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00546.x
更新日期：2008-08-01 00:00:00
abstract：:The aim of this study was to determine the relative contribution of changes (design factors) in implant system, position, bone classification, and loading condition on the biomechanical response of a single-unit implant-supported restoration. Non-linear finite-element analysis was used to simulate the mechanical respo...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2007.00473.x
更新日期：2007-10-01 00:00:00
abstract：:The aim of this study was to determine the pattern of the antibacterial activity of chlorhexidine digluconate (CHX) against mature Streptococcus mutans biofilms. Streptococcus mutans biofilms were formed on saliva-coated hydroxyapatite discs and then treated with 0-20% CHX, once, three times, or five times (1 min per ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12285
更新日期：2016-10-01 00:00:00
abstract：:A considerable number of prescriptions for antibiotics are issued by dentists. Educational institutions have a special responsibility to promote informed attitudes among dental students towards prescribing antibiotics. The purpose of this study was to analyze the antibiotic prescriptions issued during a 5-year period ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12741
更新日期：2020-12-01 00:00:00
abstract：:Secretion rates and composition of unstimulated and chewing-stimulated whole saliva and 3% citric acid stimulated parotid and submandibular-sublingual secretions were studied in 12 hypertensive patients during withdrawal of and re-exposure to antihypertensive pharmacotherapy. All the patients' blood pressures were wel...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00076.x
更新日期：1996-06-01 00:00:00
abstract：:The effect of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on bone healing was examined in calvarial defects in rabbits. Bicortical circular (critical size) defects were prepared in the calvarial bone of 16 rabbits. The defects were closed on the dural side and covered externally with expanded polyt...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00181.x
更新日期：1997-02-01 00:00:00
abstract：:The human host defense peptide, LL-37, is an important player in the first line of defense against invading microorganisms. LL-37 and its precursor, hCAP18, have been detected in unstimulated whole saliva but no reports showing hCAP18/LL-37 in isolated, parotid, and/or submandibular/sublingual saliva have been present...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12407
更新日期：2018-04-01 00:00:00
abstract：:To investigate change in oral health in relation to use of dental services, a random sample of 45- to 54-yr-old subjects from Adelaide, South Australia, was surveyed in 2004-2005 (n = 986, response rate = 44.4%). Service use and a global oral-health transition (GOHT) statement were collected over 2 yr. Worsening in or...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2012.00985.x
更新日期：2012-10-01 00:00:00
abstract：:Tooth development is regulated through a series of reciprocal interactions between the dental epithelium and mesenchyme and requires protein products of a number of genes. It has been reported that selective tooth agenesis is associated with mutations in human MSX and PAX9 genes. Mutational analysis of the two genes w...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2003.00036.x
更新日期：2003-06-01 00:00:00
abstract：:This cross-cultural study investigated adult dental fear patients in three countries. A joint intake interview questionnaire and a dental anxiety scale explored the level, background and concomitant factors of dental anxiety among patients at the Universities of Tel Aviv (Israel), Goteborg (Sweden), and Pittsburgh (US...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108004268.x
更新日期：2000-08-01 00:00:00
abstract：:At the mouse incisor tip the initially differentiated ameloblasts produce a thin, prism-free enamel, while further apically, in the immediate adjacent segment, the enamel thickness increases and the four-layered enamel of mouse incisor is formed. Comparative gene-expression profiling was carried out on RNA isolated fr...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2010.00722.x
更新日期：2010-04-01 00:00:00
abstract：:The aim of this study was to explore a possible association between health status and self-reported adverse effects related to dental amalgam restorations. A group of 50 consecutive patients (index group), referred for complaints self-related to dental amalgam restorations, was compared with a control group of individ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00206.x
更新日期：1997-06-01 00:00:00
abstract：:The Runx2 gene is a master transcription factor of bone and plays a role in all stages of bone formation. It is essential for the initial commitment of mesenchymal cells to the osteoblastic lineage and also controls the proliferation, differentiation, and maintenance of these cells. Control is complex, with involvemen...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0722.2006.00399.x
更新日期：2006-10-01 00:00:00
abstract：:Oral health problems are reported more frequently in children with disabilities, but the reasons for this are not fully known. The present study was conducted to illuminate and to gain a deeper understanding of the possible barriers preventing children with disabilities from receiving oral health care on the same prem...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2012.00961.x
更新日期：2012-06-01 00:00:00
abstract：:Plaque fluoride concentrations ([F]) are directly related to plaque calcium concentrations [Ca]. Attempts to increase plaque F uptake from dentifrices or rinses have used methods designed to increase plaque [Ca] but with inconsistent results. This double-blind, double-crossover study tested the effect of a 150 mM calc...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2006.00409.x
更新日期：2006-12-01 00:00:00
abstract：:The aim of the present study was to evaluate whether the outcome of treatment using an intraoral mandibular advancement device in patients with obstructive sleep apnoea is associated with the mandibular morphology. The effects of the device on apnoeas and sleep were evaluated in 32 men with obstructive sleep apnoea in...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1998.eos106503.x
更新日期：1998-10-01 00:00:00
abstract：:The aim of the present investigation was to map the presence of prophylactic measures in organized Swedish dental health care systems, focusing on personnel working with children and adolescents. The study was conducted by sending a questionnaire to all public dental clinics in Sweden (830 clinics in all). The results...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00115.x
更新日期：1996-08-01 00:00:00
