Abstract：
:At the mouse incisor tip the initially differentiated ameloblasts produce a thin, prism-free enamel, while further apically, in the immediate adjacent segment, the enamel thickness increases and the four-layered enamel of mouse incisor is formed. Comparative gene-expression profiling was carried out on RNA isolated from these two segments of incisor tooth germs at embryonic day (E)17.5 and at postnatal days (P)0, 1, 2, and 10 using microarrays to measure messenger RNA (mRNA) and microRNA (miRNA) species present in the segments. Validation of expression data was achieved using real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Bioinformatic data suggested enhanced cellular apoptosis in the incisal tip segment, which, together with diminished expression of the Amelx and Enam genes, may contribute to the production of the thin enamel seen in this tooth segment. For genes exhibiting higher levels of expression in the adjacent segment where complex enamel is being formed, bioinformatic analysis suggested significant associations with cellular functions involving the actin cytoskeleton, cellular development, morphology, and movement. This is suggested to reflect that ameloblasts with Tomes' process are being organized in transverse rows, facilitating the transverse movement that results in prism decussation in the inner enamel of the adjacent segment. Bioinformatic analysis of miRNA expression data lends support to these suggestions.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Sehic A,Risnes S,Khan QE,Khuu C,Osmundsen Hdoi
10.1111/j.1600-0722.2010.00722.xsubject
Has Abstractpub_date
2010-04-01 00:00:00pages
118-30issue
2eissn
0909-8836issn
1600-0722pii
EOS722journal_volume
118pub_type
杂志文章abstract：:Tooth development is regulated through a series of reciprocal interactions between the dental epithelium and mesenchyme and requires protein products of a number of genes. It has been reported that selective tooth agenesis is associated with mutations in human MSX and PAX9 genes. Mutational analysis of the two genes w...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2003.00036.x
更新日期：2003-06-01 00:00:00
abstract：:The aim of the present study was to examine pulp in amalgam-restored teeth of guinea pigs with respect to the presence of mercury, and to evaluate whether lining of cavities with resin modified glass ionomer cements had any effect on the penetration of mercury. Class V cavities were prepared in 63 incisor teeth of 21 ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2002.21367.x
更新日期：2002-12-01 00:00:00
abstract：:This study investigated the importance of dental beliefs and the predictive value of the Dental Belief Survey (DBS) in dental-fear treatment. The sample comprised 117 adult patients seeking treatment at a dental-fear clinic. Pretreatment data were collected during a screening procedure, including two visits to the den...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2003.00016.x
更新日期：2003-04-01 00:00:00
abstract：:Social support is an important phenomenon in the psychosocial work environment. The aim of this study was to assess the extent to which Danish general dental practitioners perceived support from colleagues and to relate perceived support to demographic and work related background factors. A questionnaire was sent to a...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2007.00514.x
更新日期：2008-04-01 00:00:00
abstract：:The variability of gap formation due to different operators and bonding agents around dentin-bonded composites in cylindrical cavities (internal diameter, 3 mm; depth, 1 mm; n = 210) was evaluated in vitro. Seven dentists evaluated two experimental bonding systems (A & B) and one commercial product (C) by each treatin...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.0909-8836.2003.00076.x
更新日期：2003-12-01 00:00:00
abstract：:The objective of this clinical study was to assess the internal and marginal accuracy of computer-aided design/computer-aided manufacturing (CAD/CAM)-generated four-unit all-ceramic posterior fixed dental prostheses (FDPs). The data were compared with the results of three-unit metal-ceramic and all-ceramic FDPs that w...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00580.x
更新日期：2008-12-01 00:00:00
abstract：:This study evaluated the effect of core-design modification on the characteristic strength and failure modes of glass-infiltrated alumina (In-Ceram) (ICA) compared with porcelain fused to metal (PFM). Premolar crowns of a standard design (PFMs and ICAs) or with a modified framework design (PFMm and ICAm) were fabricat...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00608.x
更新日期：2009-04-01 00:00:00
abstract：:Viridans streptococci possess a family of immunologically and structurally related cell-surface proteins, termed antigen I/II, which may function as adhesins and enable oral streptococci to adhere to saliva-coated surfaces and matrix proteins. Here we used atomic force microscopy in the molecular force mode to measure...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2010.00785.x
更新日期：2010-12-01 00:00:00
abstract：:Subsurface demineralization in enamel caries is known to entail diffusion of reagents and products both within the lesion and within the plaque biofilm external to the lesion. However, development of a predictive mathematical model for subsurface demineralization is hindered by limited quantitative understanding of th...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2005.00188.x
更新日期：2005-02-01 00:00:00
abstract：:Relearning of motor skills is important in neurorehabilitation. We investigated the improvement of training success during simple tongue protrusion (two force levels) and a more complex tongue-training paradigm using the Tongue Drive System (TDS). We also compared subject-based reports of fun, pain, fatigue, and motiv...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2011.00894.x
更新日期：2012-02-01 00:00:00
abstract：:Periodontitis progresses due to increased levels of active metalloproteinases (MMPs) and the imbalance between MMPs and their tissue inhibitors (TIMPs). Natural curcumin limits the lytic activity of MMPs but has low cellular uptake. Use of synthetic curcumin analogs could be a means of overcoming this limitation of tr...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12625
更新日期：2019-08-01 00:00:00
abstract：:In order to explore the bony changes in the mandibular body during prefunctional intraosseous eruption of premolars, 18 dogs aged from 8 to 16 wk at the beginning of experimental period, were given two intraperitoneal injections of oxytetracycline (50 mg/kg and 35 mg/kg 2 wk later) and 2 wk later a final injection of ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1998.tb02210.x
更新日期：1998-01-01 00:00:00
abstract：:This study evaluated the effect of topical fluoride application on enamel hardness after in-office bleaching. Twelve human incisors were cut along the long axis, resulting in 24 halves used in four treatment groups (n = 6 in each group): (i) untreated group (C); (ii) in-office bleaching material (B); (iii) treatment w...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12386
更新日期：2017-12-01 00:00:00
abstract：:Recombinant human BMP-7 (bone morphogenetic protein-7, osteogenic protein-1) is osteogenic, dentinogenic and cementogenic when implanted into the appropriate tissue in vivo. However, most studies characterizing the induction of these tissues have implanted BMP-7 into freshly surgerized, clinically healthy tissues. To ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108003202.x
更新日期：2000-06-01 00:00:00
abstract：:Xylitol reduces plaque but the reduction mechanism is largely unknown. The main aim of the present study was to determine whether the xylitol-induced reduction in the amount of plaque and the number of mutans streptococci could be demonstrated in subjects with (presumably) high levels of xylitol-resistant (XR; not inh...
journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1111/j.1600-0722.1997.tb00196.x
更新日期：1997-04-01 00:00:00
abstract：:This study evaluated the effect of two desensitizers on inhibition of dentin demineralization, after immersion in artificial saliva using micro-computed tomography (μCT). Dentin blocks cut from bovine incisors were treated with deionized water (DW, a negative control) or one of three desensitizers: a fluoride varnish ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12155
更新日期：2014-12-01 00:00:00
abstract：:Plaque fluoride concentrations ([F]) are directly related to plaque calcium concentrations [Ca]. Attempts to increase plaque F uptake from dentifrices or rinses have used methods designed to increase plaque [Ca] but with inconsistent results. This double-blind, double-crossover study tested the effect of a 150 mM calc...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2006.00409.x
更新日期：2006-12-01 00:00:00
abstract：:In vitro investigations of cell-specific metabolism and cell interactions as well as biocompatibility studies are often hampered by the limited lifespan of primary cells originating from target tissues like the oral mucosa, gingiva or pulp. Pulp cells, as do other primary cells, undergo senescence after several passag...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108005432.x
更新日期：2000-10-01 00:00:00
abstract：:RhoA plays a fundamental role in regulation of the actin cytoskeleton, intercellular attachment, and cell proliferation. During amelogenesis, ameloblasts (which produce the enamel proteins) undergo dramatic cytoskeletal changes and the RhoA protein level is up-regulated. Transgenic mice were generated that express a d...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12059
更新日期：2013-08-01 00:00:00
abstract：:Dentinogenesis imperfecta type II and dentin dysplasia type II are diseases resulting in abnormal dentin formation, which have been mapped to overlapping regions of human chromosome 4q defined by markers D4S2691 and D4S2692 (6.6 cM) and D4S3291 and SPP1 (14.1 cM), respectively. Recently, two of the major non-collageno...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1998.tb02180.x
更新日期：1998-01-01 00:00:00
abstract：:The objective of this study was twofold, namely to evaluate the effectiveness of a dental clinical practice guideline on the management of asymptomatic impacted lower third molars (i) on referral rates and (ii) on dentists' change in knowledge. A two-arm cluster randomized controlled trial, with pre- and post-test ass...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2005.00232.x
更新日期：2005-10-01 00:00:00
abstract：:The aims of this study were to evaluate the flexural strength of two different types of glass fibre-reinforced posts bonded to dual-cure composite resin cements. Forty glass methacrylate-based fibre posts (GC Fiber Post) and 20 glass fibre inter-polymerizing network posts (everStick POST) were divided into three group...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2010.00721.x
更新日期：2010-04-01 00:00:00
abstract：:This study compared SYBR Green real-time quantitative PCR (qPCR) with standard plate counting for the enumeration of Streptococcus mutans in oral samples. Oral samples (n = 710) were collected from high-caries-risk children for quantification of S. mutans by qPCR using primer pairs. The S. mutans copy number was calcu...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00888.x
更新日期：2011-12-01 00:00:00
abstract：:The aim of the present study was to examine to what extent pH affects human fibroblast repopulation of an experimental wound in vitro laying special emphasis on cell migration, DNA synthesis, and alterations in cell morphology. Wounds were simulated in vitro by partially scraping off monolayers of human embryonic dipl...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1995.tb00016.x
更新日期：1995-06-01 00:00:00
abstract：:Current interest in dental erosion has led to increasing attention to ways in which potentially erosive products might be modified. Information on how this could be achieved has been hard to gather, and has focused chiefly on possibilities in reformulating soft drinks. The bulk of the work published on this relates to...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0722.1996.tb00071.x
更新日期：1996-04-01 00:00:00
abstract：:The objective of this study was to investigate differences in self-perceived and dentist-determined treatment need in Australian-born and migrant residents of Australia. Participants in the National Survey of Adult Oral Health 2004-06 were categorized into six groups according to country of birth. Interview and examin...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12373
更新日期：2017-12-01 00:00:00
abstract：:The effect of the dentifrice ingredient triclosan (2,4,4'-trichloro-2'-hydroxyldiphenyl ether) on the production of interleukin (IL)-1beta and IL-6 was studied in human gingival fibroblasts challenged with tumor necrosis factor alpha (TNFalpha) in vitro. When gingival fibroblasts were treated simultaneously with tricl...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1998.eos106205.x
更新日期：1998-04-01 00:00:00
abstract：:The aim of the present study was to evaluate whether the outcome of treatment using an intraoral mandibular advancement device in patients with obstructive sleep apnoea is associated with the mandibular morphology. The effects of the device on apnoeas and sleep were evaluated in 32 men with obstructive sleep apnoea in...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1998.eos106503.x
更新日期：1998-10-01 00:00:00
abstract：:The aim of this study was to evaluate the postoperative consequences of chorda tympani reclining during middle ear surgery for otosclerosis. Electrogustometric taste thresholds were measured at 11 loci on the tongue and the soft palate in 14 patients before surgery, and 8 d, 1 month and (in some cases) 6 months after ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00556.x
更新日期：2008-10-01 00:00:00
abstract：:Salivary tissue kallikrein is stored in an active form in human salivary glands. Pre-kallikrein has been demonstrated in mixed saliva, but it is not clear if the various salivary glands contribute equally. This study set out to determine if pre-kallikrein is present in human parotid and submandibular salivas at rest, ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2001.00091.x
更新日期：2001-10-01 00:00:00
