Abstract：
:Periodontitis progresses due to increased levels of active metalloproteinases (MMPs) and the imbalance between MMPs and their tissue inhibitors (TIMPs). Natural curcumin limits the lytic activity of MMPs but has low cellular uptake. Use of synthetic curcumin analogs could be a means of overcoming this limitation of treatment efficiency. Human periodontal stem cells were isolated from gingival tissue, gingival ligament fibers, periodontal ligament, and alveolar bone. The effect of five synthetic curcumin analogs was compared with that of natural curcumin by assessing cytotoxicity [by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay], the cellular uptake (by fluorometry), the proteolytic activities of MMP-2 and -9 (by zymography), and the levels of TIMP-1 (by ELISA). Our results indicated increased cytotoxicity of synthetic curcumins for doses between 100 and 250 μM. At a concentration of 10 μM, cellular uptake of synthetic curcumins varied depending on their chemical structure. The curcumin compounds modulated pro-MMP-2 levels and increased TIMP-1 production. There was no detectable synthesis of pro-MMP-9 and no activation of MMPs 2 and 9. Gingival tissue and gingival ligament fiber stem cells were most responsive to treatment, showing inverse correlations between pro-MMP-2 and TIMP-1 levels. In conclusion, synthetic curcumins influenced the balance between pro-MMP-2 and TIMP-1 in human periodontal stem cells in vitro, and this could open perspectives for their application as adjuvants in periodontal therapy.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Boșca AB,Ilea A,Sorițău O,Tatomir C,Miklášová N,Pârvu AE,Mihu CM,Melincovici CS,Fischer-Fodor Edoi
10.1111/eos.12625subject
Has Abstractpub_date
2019-08-01 00:00:00pages
304-312issue
4eissn
0909-8836issn
1600-0722journal_volume
127pub_type
杂志文章abstract：:We sought to investigate site-specific expression of bone-regulatory factors expressed by human dental follicles and to compare the stimulated expression of tumour necrosis factor (ligand) superfamily, member 11/tumour necrosis factor receptor superfamily, member 11b (RANKL/OPG) in human dental follicle cells (HDFCs) ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12568
更新日期：2018-12-01 00:00:00
abstract：:The aim of this investigation was to study the intraoral pH response on tooth surfaces in relation to dental erosion during and after drinking a sugar-free cola-type soft drink. Six different methods of drinking were tested in a randomized order: holding; short-sipping; long-sipping; gulping; nipping; and sucking. Two...
journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2004.00172.x
更新日期：2004-12-01 00:00:00
abstract：:Paraffin-stimulated saliva samples were collected from 140 children 3- and 4-yr old attending nine nursery schools in Latvia. The salivary levels of mutans streptococci were rated from zero to 3 after being cultured on a commercially available strip selective for these microorganisms. Of the children, 29.3% were rated...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1995.tb00171.x
更新日期：1995-08-01 00:00:00
abstract：:The present study investigated whether daily systemic administration of docosahexaenoic acid (DHA) in rats could attenuate the hyperexcitability of trigeminal spinal nucleus caudalis (SpVc) neurons associated with hyperalgesia. Inflammation was induced in rats by injecting complete Freund's adjuvant into the whisker p...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12571
更新日期：2018-12-01 00:00:00
abstract：:This study measured the polymerization contraction of a resin composite cured at three levels of energy density, each attained at six different levels of power density. The polymerization contraction of the composite was recorded by the method of the deflecting disc (n = 5) for 1 h following the start of irradiation. ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2005.00239.x
更新日期：2005-10-01 00:00:00
abstract：:The aim of this study was to explore a possible association between health status and self-reported adverse effects related to dental amalgam restorations. A group of 50 consecutive patients (index group), referred for complaints self-related to dental amalgam restorations, was compared with a control group of individ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00206.x
更新日期：1997-06-01 00:00:00
abstract：:We aimed to evaluate (i) changes in dental fear over time during pregnancy and after delivery among mothers and fathers and (ii) whether these changes inter-relate to changes in depression and anxiety. Longitudinal pilot data for the FinnBrain Cohort study were used. Of 254 pregnant families, 80% agreed to participate...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12026
更新日期：2013-06-01 00:00:00
abstract：:Human dentin mineral has been investigated by using micro-Raman spectroscopy. Fluorescence and thermal problems were largely avoided by preparing dentin samples by grinding and ultrasonic agitation in acetone. The Raman spectral features were consistent with those of impure hydroxyapatite containing CO3 and HPO4. Whil...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00056.x
更新日期：1996-04-01 00:00:00
abstract：:Plaque fluoride concentrations ([F]) are directly related to plaque calcium concentrations [Ca]. Attempts to increase plaque F uptake from dentifrices or rinses have used methods designed to increase plaque [Ca] but with inconsistent results. This double-blind, double-crossover study tested the effect of a 150 mM calc...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2006.00409.x
更新日期：2006-12-01 00:00:00
abstract：:To explore the intrafamilial homology and longitudinal stability of colonization by early acquired mutans streptococci, genomic fingerprinting was performed on 254 strains (192 Streptococcus mutans and 62 Streptococcus sobrinus strains) collected from 16 families (16 mother-child pairs, seven fathers and four siblings...
journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1034/j.1600-0722.2003.00068.x
更新日期：2003-10-01 00:00:00
abstract：:Streptococcus oligofermentans is a novel strain of oral streptococcus that can specifically inhibit the growth of Streptococcus mutans. The aims of this study were to assess the growth of S. oligofermentans and the ability of S. oligofermentans to inhibit growth of Streptococcus mutans at different pH values. Growth i...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12102
更新日期：2014-02-01 00:00:00
abstract：:Enamelysin is a matrix metalloproteinase (MMP-20) secreted by ameloblasts, previously shown to hydrolyze recombinant amelogenin. The purpose of this study was to use recombinant MMP-20 to further investigate the specific hydrolysis of peptide fragments containing cleavage sites for tyrosine-rich amelogenin peptide (TR...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1999.eos107506.x
更新日期：1999-10-01 00:00:00
abstract：:It was the purpose of this article to analyze the (micro) morphological structure of enamel at different stages of development in order to deduce movement patterns of ameloblasts during formation of the human dental primordium. Developing enamel and overlying ameloblasts were dried and fractured for scanning electron ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2006.00314.x
更新日期：2006-05-01 00:00:00
abstract：:Recent thinking about the role of regional socioeconomic context in generating health inequalities has argued for the collection of true 'area' data that accurately reflect the characteristics of a region. We investigated whether a range of attributes of regional environments, constructed from factor analysis of vario...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00831.x
更新日期：2011-08-01 00:00:00
abstract：:The aim of this study was to determine the relative contribution of changes (design factors) in implant system, position, bone classification, and loading condition on the biomechanical response of a single-unit implant-supported restoration. Non-linear finite-element analysis was used to simulate the mechanical respo...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2007.00473.x
更新日期：2007-10-01 00:00:00
abstract：:Severe pain after third molar surgery is often encountered and more effective treatment regimes are warranted. The objective of this study was to evaluate if the combination of paracetamol and rofecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, improves analgesic effects following third molar surgery compared w...
journal_title：European journal of oral sciences
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1111/j.1600-0722.2006.00365.x
更新日期：2006-08-01 00:00:00
abstract：:Fast eating has been shown to increase the risk of overweight in both children and adults. The objectives of the present study were to investigate the correlation between chewing rate and the number of chews per mouthful and to evaluate if they were associated with the weight of meal intake. Thirty healthy subjects, a...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12583
更新日期：2019-02-01 00:00:00
abstract：:Our previous study showed that p53 protein is accumulated in >60% of cases of oral squamous cell carcinoma (OSCC) and its overexpression is related to poor prognosis. However, the mechanism behind this is still elusive. The present study attempts to dissect p53 alterations at various levels from gene to function in tu...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12007
更新日期：2013-02-01 00:00:00
abstract：:Stimulated human whole saliva (WS) was used to study the dynamics of papain hydrolysis at defined pH, ionic strength, and temperature with the view of reducing an acquired pellicle. A quartz crystal microbalance with dissipation (QCM-D) was used to monitor the changes in frequency caused by enzyme hydrolysis of WS fil...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2012.00942.x
更新日期：2012-04-01 00:00:00
abstract：:The formation of dental enamel is a prototype of functional tissue development through biomineralization. Amelotin (AMTN) is a recently discovered secreted enamel protein predominantly expressed during the maturation stage of enamel formation. It accumulates in a basal lamina-like structure at the interface between am...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2011.00870.x
更新日期：2011-12-01 00:00:00
abstract：:The aim of this study was to assess post-trial treatment costs, clinical outcomes [decayed, missing or filled surfaces (DMFS) scores], and utilization of dental services among adolescents who had participated in a randomized clinical trial (RCT) in Pori, Finland, in 2001-2005. At baseline the children were 11-12 yr of...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2010.00726.x
更新日期：2010-06-01 00:00:00
abstract：:The effect of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on bone healing was examined in calvarial defects in rabbits. Bicortical circular (critical size) defects were prepared in the calvarial bone of 16 rabbits. The defects were closed on the dural side and covered externally with expanded polyt...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00181.x
更新日期：1997-02-01 00:00:00
abstract：:Although interproximal attrition is considered to be limited in modern populations, it has important clinical implications. However, in contrast to occlusal attrition, proximal attrition receives limited scientific attention. The main purpose of the current study was to fill this void. Seven-hundred and sixty-five tee...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12135
更新日期：2014-08-01 00:00:00
abstract：:Improving access to National Health Service (NHS) dentistry is a public health issue that has been a focus for successive governments and policy makers in the UK. To inform this process, commissioners of services need to understand trends in service use and demands of the local population. This study explored inequali...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12043
更新日期：2013-06-01 00:00:00
abstract：:The aim of this study was to investigate the effect of polymerization time and polymerization temperature on the residual methyl methacrylate (MMA) content of two heat-cured and two autopolymerized denture base polymers. Gas chromatography was used to determine the residual MMA content of three test specimens of each ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1998.eos106109.x
更新日期：1998-02-01 00:00:00
abstract：:This study investigated the effect of repeated tongue motor tasks on suprahyoid muscle activity and tongue pressure. Fourteen participants performed three series of a standardized tongue-lift training (TLT) task on each of five consecutive days. Electromyographic (EMG) activity from suprahyoid muscles and tongue press...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12300
更新日期：2016-12-01 00:00:00
abstract：:Subcutaneous implantation of bone morphogenetic protein (BMP) combined with a fibrous glass membrane (FGM) induces cartilage formation in the entire inner area of the membrane within 2 wk. It has been hypothesized that a tight FGM network (1 microm exclusion size) provides immature cells with spaces for penetrating in...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1997.tb00222.x
更新日期：1997-12-01 00:00:00
abstract：:Dentinogenesis imperfecta type II and dentin dysplasia type II are diseases resulting in abnormal dentin formation, which have been mapped to overlapping regions of human chromosome 4q defined by markers D4S2691 and D4S2692 (6.6 cM) and D4S3291 and SPP1 (14.1 cM), respectively. Recently, two of the major non-collageno...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1998.tb02180.x
更新日期：1998-01-01 00:00:00
abstract：:Proteolytic enzymes with gelatinolytic activity in the synovial fluid (SF) of temporomandibular joint (TMJ) arthropathies were assayed by gelatin-impregnated gel enzymography. SF samples were collected from 10 TMJs in patients with closed lock (CL) condition and 5 TMJs from asymptomatic healthy volunteers. Two protein...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1998.eos106603.x
更新日期：1998-12-01 00:00:00
abstract：:This study evaluated the effect of two desensitizers on inhibition of dentin demineralization, after immersion in artificial saliva using micro-computed tomography (μCT). Dentin blocks cut from bovine incisors were treated with deionized water (DW, a negative control) or one of three desensitizers: a fluoride varnish ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12155
更新日期：2014-12-01 00:00:00
