Abstract：
:The formation of dental enamel is a prototype of functional tissue development through biomineralization. Amelotin (AMTN) is a recently discovered secreted enamel protein predominantly expressed during the maturation stage of enamel formation. It accumulates in a basal lamina-like structure at the interface between ameloblasts and enamel mineral and it co-localizes with another recently described enamel protein, odontogenic ameloblast-associated protein (ODAM). The purpose of this study was to determine whether AMTN and ODAM bind to each other and/or to other well-established enamel matrix proteins. The coding sequences of all enamel proteins were cloned into appropriate vectors of the GAL4-based Matchmaker Gold Yeast Two-Hybrid System. The growth of yeast cells on selective media and color induction were used as indicators for reporter gene expression through protein-protein interactions in combinations of prey and bait constructs. We found that AMTN interacts with itself and with ODAM, but not with amelogenin (AMEL), ameloblastin (AMBN), or enamelin (ENAM). Using ODAM as bait, the interaction with AMTN was confirmed. Furthermore, ODAM was found to bind to itself and to AMBN, as well as weakly to AMEL but not to ENAM. We propose a model where the distinct expression of AMTN and ODAM and their interaction are involved in defining the enamel microstructure at the enamel surface.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Holcroft J,Ganss Bdoi
10.1111/j.1600-0722.2011.00870.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
301-6eissn
0909-8836issn
1600-0722journal_volume
119 Suppl 1pub_type
杂志文章abstract：:The demineralization of dentin was studied when placed adjacent to one of four experimental denture base-resins. These experimental resins contained polymethylmethacrylate (PMMA) and 0, 5, 10, 20 or 30 wt% surface reaction-type prereacted glass-ionomer (S-PRG) filler, respectively. A dentin thin-section was sandwiched...
journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
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doi：10.1111/j.1600-0722.1996.tb00039.x
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108005432.x
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2005.00232.x
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abstract：:Amelogenesis imperfecta is a group of hereditary enamel defects. Of the autosomal dominant forms, only the local hypoplastic type has been mapped to human chromosome 4q 13-4q21. Enamelin is a large enamel matrix protein secreted by ameloblasts. The purpose of this study was to determine the human chromosomal localizat...
journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2004.00138.x
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00608.x
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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更新日期：2013-12-01 00:00:00
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
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更新日期：2007-12-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108004341.x
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journal_title：European journal of oral sciences
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doi：10.1046/j.0909-8836.1998.eos106205.x
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
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abstract：:Plaque surface area after rinsing with a tea, containing low levels of fluoride, was evaluated in 30 human volunteers with a single-blind, one-operator, three-period, three-treatment cross-over study. The mouthwashes employed were: (1) tea (Darjeeling first flush:< 0.2 ppm F-), (2) Meridol(R), (3) control (tap water:<...
journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
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更新日期：1995-12-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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更新日期：2009-12-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00546.x
更新日期：2008-08-01 00:00:00
abstract：:Root resorption appears to be an inevitable sequel to orthodontic tooth movement, occurring either apically or along surfaces in and around the pressure zones. However, there is uncertainty as to the location of initial cementum repair in resorbed areas as well as the time of onset of repair. The aim of the present st...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1995.tb00029.x
更新日期：1995-10-01 00:00:00
abstract：:An oxygen inhibition layer develops on surfaces exposed to air during polymerization of particulate filling composite. This study assessed the thickness of the oxygen inhibition layer of short-fiber-reinforced composite in comparison with conventional particulate filling composites. The effect of an oxygen inhibition ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/eos.12167
更新日期：2015-02-01 00:00:00
abstract：:Temporomandibular joint (TMJ) histology was investigated in 8 female Scandinavian moose. 5 were 1-year-old with a carcass weight (cw) of 125-140 kg, and 3 were 2-years-old (160-175 kg cw). The condylar articular surface consisted of a connective tissue lining with parallel collagen fibres. Numerous blood vessels were ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00146.x
更新日期：1996-10-01 00:00:00
