Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro.

Abstract:

:Ozone gas and photodynamic therapy (PDT) have been claimed to be antimicrobially effective. This study assessed their antimicrobial potential in vitro. Mature six-species oral biofilms were treated as follows (n = 9 per group): (i) a 60-s application of gasiform vacuum-ozone or vacuum alone (on wet or air-dried biofilm samples); (ii) PDT (i.e. methylene blue in combination with or without a diode soft laser, and a soft laser alone); or (iii) antimicrobial solutions: immersion of biofilms for 60 s in 0.2 and 2% chlorhexidine or in 0.5 and 5% hypochlorite solution. Treatment with chlorhexidine or hypochlorite served as a positive control, whereas untreated samples served as negative controls. Colony-forming units on blood agar were counted. Only the 5% hypochlorite solution was able to totally eliminate the microorganisms in the biofilm. The observed reduction of viable counts by vacuum-ozone application and PDT was less than one log(10) step. Under the conditions of the current study, gasiform ozone and PDT had a minimal effect on the viability of microorganisms organized in a cariogenic biofilm.

journal_name

Eur J Oral Sci

authors

Müller P,Guggenheim B,Schmidlin PR

doi

10.1111/j.1600-0722.2007.00418.x

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

77-80

issue

1

eissn

0909-8836

issn

1600-0722

pii

EOS418

journal_volume

115

pub_type

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