Abstract:
:Our previous study showed that p53 protein is accumulated in >60% of cases of oral squamous cell carcinoma (OSCC) and its overexpression is related to poor prognosis. However, the mechanism behind this is still elusive. The present study attempts to dissect p53 alterations at various levels from gene to function in tumor biopsies to understand whether molecular alterations have any relationship with the accumulation of p53 protein in OSCC. Protein-stabilizing mutations were observed in only 13.8% of the samples. Neither p53 polymorphisms nor human papillomavirus (HPV) infection (<2%) has any major role in augmented expression of p53 protein. Analysis of the p53 transcript demonstrated that alteration in the level of p53 mRNA (10%) is not the major mechanistic cause for accumulation of p53 protein, and p21 transcript status showed that the overexpressed p53 protein is functionally inactive. Immunoprecipitation studies demonstrated that the known interactors of p53, such as MDM2 and HSP70, have no significant role in stabilizing p53 and the significant presence of some unknown interactors, sequestering p53, and leading to the aberrant accumulation of p53. Our study suggests that overexpression of inactive p53 protein could be manifested as a result of sequestering from degradation by an unknown interacting protein rather than by gene or transcript level of alteration.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Francis G,Dileep Kumar U,Nalinakumari KR,Jayasree K,Kannan Sdoi
10.1111/eos.12007subject
Has Abstractpub_date
2013-02-01 00:00:00pages
21-8issue
1eissn
0909-8836issn
1600-0722journal_volume
121pub_type
杂志文章abstract::The formation of dental enamel is a prototype of functional tissue development through biomineralization. Amelotin (AMTN) is a recently discovered secreted enamel protein predominantly expressed during the maturation stage of enamel formation. It accumulates in a basal lamina-like structure at the interface between am...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2011.00870.x
更新日期:2011-12-01 00:00:00
abstract::This study tested the fluoride-release rate and the root caries inhibitory effect of dental adhesives. In phase 1, the fluoride released from samples (n = 5) of the adhesives A (Optibond Solo), B (One-up Bond F), C (Prime & Bond NT), D (Tenure Quick), and also of the controls [+] (glass-ionomer cement) and [-] (non-fl...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2005.00214.x
更新日期:2005-06-01 00:00:00
abstract::To explore the intrafamilial homology and longitudinal stability of colonization by early acquired mutans streptococci, genomic fingerprinting was performed on 254 strains (192 Streptococcus mutans and 62 Streptococcus sobrinus strains) collected from 16 families (16 mother-child pairs, seven fathers and four siblings...
journal_title:European journal of oral sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1034/j.1600-0722.2003.00068.x
更新日期:2003-10-01 00:00:00
abstract::In the present study the crystalline phases of calcium sulphate present in various commercially available alginate impression materials were identified by means of x-ray diffraction. The diffractograms obtained indicated that the predominant crystalline phase in most materials was calcium sulphate dihydrate and confir...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1996.tb00156.x
更新日期:1996-10-01 00:00:00
abstract::The Runx2 gene is a master transcription factor of bone and plays a role in all stages of bone formation. It is essential for the initial commitment of mesenchymal cells to the osteoblastic lineage and also controls the proliferation, differentiation, and maintenance of these cells. Control is complex, with involvemen...
journal_title:European journal of oral sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0722.2006.00399.x
更新日期:2006-10-01 00:00:00
abstract::Immunoexpression of the extracellular matrix (ECM) proteins laminin, fibronectin, tenascin and types I, III and IV collagen was analyzed in the major and minor salivary glands of seven human fetuses at different gestational ages. The results showed the presence and localization of laminin, collagen IV and fibronectin ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2004.00169.x
更新日期:2004-12-01 00:00:00
abstract::The aims were to test the hypotheses that experimental masseter muscle pain leads to recruitment and/or derecruitment of motor units at different sites within the masseter and that the patterns of change in motor unit activity differ between sites. Single motor unit (SMU) activity was recorded at two sites within the ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12561
更新日期:2018-10-01 00:00:00
abstract::Recombinant human BMP-7 (bone morphogenetic protein-7, osteogenic protein-1) is osteogenic, dentinogenic and cementogenic when implanted into the appropriate tissue in vivo. However, most studies characterizing the induction of these tissues have implanted BMP-7 into freshly surgerized, clinically healthy tissues. To ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2000.108003202.x
更新日期:2000-06-01 00:00:00
abstract::In the present study we investigated the prevalence of self-reported hand eczema as well as subjective associations between skin symptoms and composite/bonding or other dental materials among Swedish dentists. A questionnaire on skin symptoms, atopy, occupational experience, and other background factors was sent to a ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1046/j.0909-8836.1999.eos107612.x
更新日期:1999-12-01 00:00:00
abstract::We assessed the effect of certain oral health-related behaviours on adults' dental health. As part of the Finnish nationwide Health 2000 Survey, dentate subjects, 30-64 yr of age, reported their frequency of consumption of eight sugar- and xylitol-containing products, together with toothbrushing frequency and use of f...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2010.00804.x
更新日期:2011-02-01 00:00:00
abstract::Dentinogenesis imperfecta type II and dentin dysplasia type II are diseases resulting in abnormal dentin formation, which have been mapped to overlapping regions of human chromosome 4q defined by markers D4S2691 and D4S2692 (6.6 cM) and D4S3291 and SPP1 (14.1 cM), respectively. Recently, two of the major non-collageno...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1998.tb02180.x
更新日期:1998-01-01 00:00:00
abstract::Saliva contacting with solid surfaces in the oral cavity forms a coat termed the pellicle. However, its formation is not fully understood. Although indications for the existence of supramolecular pellicle precursors have been reported, the possible relationship between them and pellicle formation is unclear. This stud...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2004.00138.x
更新日期:2004-08-01 00:00:00
abstract::Streptococcus oligofermentans is a novel strain of oral streptococcus that can specifically inhibit the growth of Streptococcus mutans. The aims of this study were to assess the growth of S. oligofermentans and the ability of S. oligofermentans to inhibit growth of Streptococcus mutans at different pH values. Growth i...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12102
更新日期:2014-02-01 00:00:00
abstract::This study aimed to create a thick hydroxyapatite (HA) film on the surface of a human tooth via a powder jet deposition (PJD) device for dental handpieces, and to examine the microstructural and mechanical properties of the HA film. In particular, the effects of thermal stress on this film were evaluated. The HA film ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12076
更新日期:2013-10-01 00:00:00
abstract::Originally identified as the active components within osteoinductive extracts derived from bone, the BMPs now are known to include a large family of proteins within the TGF-beta superfamily of growth and differentiation factors. Members of the BMP family have been determined to be key signaling molecules in embryogene...
journal_title:European journal of oral sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0722.1998.tb02170.x
更新日期:1998-01-01 00:00:00
abstract::The aim of this in vitro study was to investigate the relationship between bacterial infections in carious dentin when detected by two different methods -- polymerase chain reaction (PCR), and a laser fluorescence device, DIAGNOdent. Dentin was removed every 300 micro m in the direction of the pulp chamber in 10 extra...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2004.00161.x
更新日期:2004-10-01 00:00:00
abstract::Recent thinking about the role of regional socioeconomic context in generating health inequalities has argued for the collection of true 'area' data that accurately reflect the characteristics of a region. We investigated whether a range of attributes of regional environments, constructed from factor analysis of vario...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2011.00831.x
更新日期:2011-08-01 00:00:00
abstract::The overall aim of this study was to examine the relationship between bone mass of the jaw and the axial skeleton. Sixty-nine subjects (age at entry: 32-64), who underwent hormone replacement therapy during the study period, participated. Bone mass of the lumbar spine was determined using dual photon absorptiometry. T...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1996.tb00039.x
更新日期:1996-02-01 00:00:00
abstract::The purpose of this study was to compare fluid flow rates across dentin surfaces treated with four conditioners. The effect of conditioning on the micro-shear bond strengths of glass ionomer cement (Fuji IX GP) and resin-based adhesives (Single Bond 2 or Clearfil SE Bond) were also investigated. Under a simulated pres...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2007.00483.x
更新日期:2007-12-01 00:00:00
abstract::Dental personnel and orthopedic surgeons are at risk when manually handling products containing methyl methacrylate (MMA). Dental products may also contain cross-linking agents such as ethylene glycol dimethacrylate (EGDMA) or 1,4-butanediol dimethacrylate (1,4-BDMA). Skin contact with monomers can cause hand eczema, ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1034/j.1600-0722.2003.00064.x
更新日期:2003-10-01 00:00:00
abstract::The aim of this study was to visualize fluid movement through dental enamel in vivo. Fifty permanent upper central incisors, from subjects aged 10-70 yr, and 5 permanent central just-erupted incisors, from subjects aged 6-7 yr, were included in the study. An impression was obtained by vinyl polyxiloxane, and replicas ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2007.00445.x
更新日期:2007-06-01 00:00:00
abstract::Subcutaneous implantation of bone morphogenetic protein (BMP) combined with a fibrous glass membrane (FGM) induces cartilage formation in the entire inner area of the membrane within 2 wk. It has been hypothesized that a tight FGM network (1 microm exclusion size) provides immature cells with spaces for penetrating in...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1997.tb00222.x
更新日期:1997-12-01 00:00:00
abstract::Human dental plaque is thought to contribute to disease, not only in the oral cavity but also at other body sites. To investigate the pathogenicity of dental plaque in tissues remote from the mouth, we examined the ability of human supragingival dental plaque to induce infective endocarditis (IE) in rats. In total, 15...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2005.00258.x
更新日期:2005-12-01 00:00:00
abstract::Plaque fluoride concentrations ([F]) are directly related to plaque calcium concentrations [Ca]. Attempts to increase plaque F uptake from dentifrices or rinses have used methods designed to increase plaque [Ca] but with inconsistent results. This double-blind, double-crossover study tested the effect of a 150 mM calc...
journal_title:European journal of oral sciences
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-0722.2006.00409.x
更新日期:2006-12-01 00:00:00
abstract::Social support is an important phenomenon in the psychosocial work environment. The aim of this study was to assess the extent to which Danish general dental practitioners perceived support from colleagues and to relate perceived support to demographic and work related background factors. A questionnaire was sent to a...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2007.00514.x
更新日期:2008-04-01 00:00:00
abstract::So-called secondary caries may develop in the cement gap between the tooth and the bonded restoration. Cement materials with a low susceptibility to biofilm formation are therefore desirable. In the present study, the adhesion of Strepococcus mutans onto three adhesive (Multilink Automix, RelyX Ultimate, and Panavia V...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12355
更新日期:2017-08-01 00:00:00
abstract::The potential problems of DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples and amplification efficiency of Human papilloma virus (HPV) may occur in the molecular studies of head and neck squamous carcinoma (HNSCC). The aim of this study was to compare HPV detection rate in FFPE tissues of ora...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12746
更新日期:2020-12-01 00:00:00
abstract::This study aimed to investigate the effect of glutamate-evoked masseter muscle pain on intramuscular oxygenation during rest and sustained elevated muscle activity (SEMA). Seventeen healthy individuals participated in two sessions in which they were injected with glutamate and saline in random order. Each session was ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/eos.12383
更新日期:2017-12-01 00:00:00
abstract::Human dentin mineral has been investigated by using micro-Raman spectroscopy. Fluorescence and thermal problems were largely avoided by preparing dentin samples by grinding and ultrasonic agitation in acetone. The Raman spectral features were consistent with those of impure hydroxyapatite containing CO3 and HPO4. Whil...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.1996.tb00056.x
更新日期:1996-04-01 00:00:00
abstract::This study measured the polymerization contraction of a resin composite cured at three levels of energy density, each attained at six different levels of power density. The polymerization contraction of the composite was recorded by the method of the deflecting disc (n = 5) for 1 h following the start of irradiation. ...
journal_title:European journal of oral sciences
pub_type: 杂志文章
doi:10.1111/j.1600-0722.2005.00239.x
更新日期:2005-10-01 00:00:00