Abstract:
:Human dentin mineral has been investigated by using micro-Raman spectroscopy. Fluorescence and thermal problems were largely avoided by preparing dentin samples by grinding and ultrasonic agitation in acetone. The Raman spectral features were consistent with those of impure hydroxyapatite containing CO3 and HPO4. While spectral differences between enamel and dentin were clearly observable as changes in the bandwidth of the PO4(3-)V1 band and the intensities of the OH-, CO3(2-) and HPO4(2-) bands, the technique could not detect spectral differences between coronal and root dentin. NaOCl, NaF and APF-gel treatments caused measurable changes in intensities of the bands due to CO3(2-) and HPO4(2-); the results were found to be useful for band assignments. After NaOCl treatment, the Raman bands, presumably due to amide and HPO4(2-), were lost, but the band intensity of the CO3(2-)V1 bands increased by 35-60%. This increase coincided with the appearance of a new broad band (250-300 cm-1). The same treatment on enamel caused no increase in the CO3(2-)V1 band intensity. This NaOCl-induced carbonate could be removed within 20 h in a 1000 ppm NaF solution. These findings indicate that the carbonate ions induced by the NaOCl treatment are presumably in or on the mineral surface. After 3 min of APF-gel treatment on NaOCl-pretreated dentin, the intensities of the hydroxyapatite phosphate bands dropped by approximately 20%, and newly formed CaF2 and HPO4 bands became observable.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Tsuda H,Ruben J,Arends Jdoi
10.1111/j.1600-0722.1996.tb00056.xsubject
Has Abstractpub_date
1996-04-01 00:00:00pages
123-31issue
2 ( Pt 1)eissn
0909-8836issn
1600-0722journal_volume
104pub_type
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journal_title:European journal of oral sciences
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journal_title:European journal of oral sciences
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:European journal of oral sciences
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journal_title:European journal of oral sciences
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