Abstract：
:Kallikrein-related peptidase 4 (KLK4) is critical for proper dental enamel formation. Klk4 null mice, and humans with two defective KLK4 alleles have obvious enamel defects, with no other apparent phenotype. KLK4 mRNA or protein is reported to be present in tissues besides teeth, including prostate, ovary, kidney, liver, and salivary gland. In this study we used the Klk4 knockout/NLS-lacZ knockin mouse to assay Klk4 expression using β-galactosidase histochemistry. Incubations for 5 h were used to detect KLK4 expression with minimal endogenous background, while overnight incubations susceptible to false positives were used to look for trace KLK4 expression. Developing maxillary molars at postnatal days 5, 6, 7, 8, and 14, developing mandibular incisors at postnatal day 14, and selected non-dental tissues from adult wild-type and Klk4(lacZ/lacZ) mice were examined by X-gal histochemistry. After 5 h of incubation, X-gal staining was observed specifically in the nuclei of maturation-stage ameloblasts in molars and incisors from Klk4(lacZ/lacZ) mice and was detected weakly in the nuclei of salivary gland ducts and in patches of prostate epithelia. We conclude that KLK4 is predominantly a tooth-specific protease with low expression in submandibular salivary gland and prostate, and with no detectable expression in liver, kidney, testis, ovary, oviduct, epididymis, and vas deferens.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Simmer JP,Richardson AS,Smith CE,Hu Y,Hu JCdoi
10.1111/j.1600-0722.2011.00834.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
226-33eissn
0909-8836issn
1600-0722journal_volume
119 Suppl 1pub_type
杂志文章abstract：:Current interest in dental erosion has led to increasing attention to ways in which potentially erosive products might be modified. Information on how this could be achieved has been hard to gather, and has focused chiefly on possibilities in reformulating soft drinks. The bulk of the work published on this relates to...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0722.1996.tb00071.x
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abstract：:Comminution of food is the composite result of selection and breakage. Selection is characterised by the chance that a food particle will at least be damaged by the teeth during chewing. For any size, this chance equals the ratio between the weight of damaged or broken particles and that of all initial particles. The ...
journal_title：European journal of oral sciences
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doi：10.1034/j.1600-0722.2001.00092.x
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章
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更新日期：1995-12-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章,多中心研究
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更新日期：2008-12-01 00:00:00
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pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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更新日期：2007-02-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
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abstract：:The aim of this study was to evaluate and to test the psychometric properties of a Swedish version of the revised Dental Beliefs Survey (DBS-R) in different patient groups and in a non-clinical sample of students. It was hypothesized that negative dental beliefs, assessed using the DBS-R, would discriminate between fe...
journal_title：European journal of oral sciences
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abstract：:Dentin bonding using the total-etch method has been claimed to be technique-sensitive. The aim of this study is to examine the effect of acid-etch variations on the dentin demineralization and interfacial structure of the adhesive-dentin bond using a differential staining technique. Single Bond adhesive with 35% phosp...
journal_title：European journal of oral sciences
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abstract：:The objectives of this study were to determine the prevalence, intensity, and extent of the impacts caused by oral problems, and to compare their intensity and extent by the type of oral problem. Four of seven public schools linked to a Health Center in Lima, Peru, were randomly selected as clusters, and 805 11-12-yr-...
journal_title：European journal of oral sciences
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