Abstract：
:Hamster enamel protein extracts were analyzed by RP-HPLC and the isolated fractions by SDS-and Western blotting using polyclonal antibodies against recombinant mouse amelogenin and anti-peptide antibodies against the mouse exon 4-encoded sequence. Total RNA was extracted from enamel organ epithelia and, using a 3' rapid amplification of cDNA ends (3' RACE) technique, the coding regions for three different amelogenin isoforms were cloned along with the 3' non-coding region. DNA sequencing revealed that the hamster amelogenin isoforms are 180, 73 and 59 amino acids in length, respectively. The 59-residue amelogenin corresponds to the leucine-rich amelogenin protein (LRAP), the 73-residue amelogenin corresponds to LRAP with the inclusion of the exon 4-encoded sequence, while the 180-residue amelogenin is the most abundant amelogenin isoform. Edman degradation was performed on purified hamster amelogenin, which provided the amino acid sequence in the region encoded by the 5' PCR amplification primer used in cloning. Therefore, the entire derived amino acid sequence of hamster amelogenin was revealed. The hamster amelogenin amino acid sequence was aligned with all its known homologues. Hamster differs from rat and mouse amelogenin at only three amino acid positions. Southern blot analysis using a panel of restriction enzymes gave the same pattern for hamster DNA obtained from males and females, suggesting that in hamster, as in mouse, amelogenin is expressed from a single gene located on the X chromosome.
journal_name
Eur J Oral Scijournal_title
European journal of oral sciencesauthors
Lyaruu DM,Hu CC,Zhang C,Qian Q,Ryu OH,Moradian-Oldak J,Wöltgens JH,Fincham AG,Simmer JPdoi
10.1111/j.1600-0722.1998.tb02190.xsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
299-307eissn
0909-8836issn
1600-0722journal_volume
106 Suppl 1pub_type
杂志文章abstract：:The aim of this study was to validate the use of digitized periapical radiographs in evaluating orthodontically induced apical root resorption against micro-computed tomography (micro-CT) scanning as a criterion standard test. In a standardized experimental protocol, 29 premolars in 16 subjects were tipped buccally fo...
journal_title：European journal of oral sciences
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1600-0722.2008.00559.x
更新日期：2008-10-01 00:00:00
abstract：:Ozone gas and photodynamic therapy (PDT) have been claimed to be antimicrobially effective. This study assessed their antimicrobial potential in vitro. Mature six-species oral biofilms were treated as follows (n = 9 per group): (i) a 60-s application of gasiform vacuum-ozone or vacuum alone (on wet or air-dried biofil...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2007.00418.x
更新日期：2007-02-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2004.00127.x
更新日期：2004-06-01 00:00:00
abstract：:The Runx2 gene is a master transcription factor of bone and plays a role in all stages of bone formation. It is essential for the initial commitment of mesenchymal cells to the osteoblastic lineage and also controls the proliferation, differentiation, and maintenance of these cells. Control is complex, with involvemen...
journal_title：European journal of oral sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0722.2006.00399.x
更新日期：2006-10-01 00:00:00
abstract：:Oral health problems are reported more frequently in children with disabilities, but the reasons for this are not fully known. The present study was conducted to illuminate and to gain a deeper understanding of the possible barriers preventing children with disabilities from receiving oral health care on the same prem...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2012.00961.x
更新日期：2012-06-01 00:00:00
abstract：:It has been reported that phosphoric acid (PA) produces structural and molecular alterations in dentin collagen fibrils; however, no relevant information exists on the influence of etching with PA on dentin non-collagenous macromolecules. The present study investigated, by immunohistochemistry and ultrastructural hist...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.00912.x
更新日期：2000-12-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1996.tb00115.x
更新日期：1996-08-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.00791.x
更新日期：2000-04-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章,多中心研究,随机对照试验
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章
doi：10.1111/j.1600-0722.1995.tb01857.x
更新日期：1995-12-01 00:00:00
abstract：:In order to explore the bony changes in the mandibular body during prefunctional intraosseous eruption of premolars, 18 dogs aged from 8 to 16 wk at the beginning of experimental period, were given two intraperitoneal injections of oxytetracycline (50 mg/kg and 35 mg/kg 2 wk later) and 2 wk later a final injection of ...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1998.tb02210.x
更新日期：1998-01-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1046/j.0909-8836.1999.eos107108.x
更新日期：1999-02-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2002.11171.x
更新日期：2002-04-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108005432.x
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abstract：:The aim of this investigation was to study the intraoral pH response on tooth surfaces in relation to dental erosion during and after drinking a sugar-free cola-type soft drink. Six different methods of drinking were tested in a randomized order: holding; short-sipping; long-sipping; gulping; nipping; and sucking. Two...
journal_title：European journal of oral sciences
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：European journal of oral sciences
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.1995.tb00171.x
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2010.00785.x
更新日期：2010-12-01 00:00:00
abstract：:As a result of their presence throughout the mouth in the submucosa or between muscle fibers, minor salivary glands secrete directly and continuously into the oral cavity, providing mucosal surfaces with highly glycosylated proteins that are active in bacterial aggregation and in oral tissue lubrication. In this study...
journal_title：European journal of oral sciences
pub_type： 杂志文章
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更新日期：2010-02-01 00:00:00
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journal_title：European journal of oral sciences
pub_type： 杂志文章
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更新日期：1998-08-01 00:00:00
abstract：:Amelogenesis imperfecta is a group of hereditary enamel defects. Of the autosomal dominant forms, only the local hypoplastic type has been mapped to human chromosome 4q 13-4q21. Enamelin is a large enamel matrix protein secreted by ameloblasts. The purpose of this study was to determine the human chromosomal localizat...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1034/j.1600-0722.2000.108005353.x
更新日期：2000-10-01 00:00:00
abstract：:This study evaluated the effect of core-design modification on the characteristic strength and failure modes of glass-infiltrated alumina (In-Ceram) (ICA) compared with porcelain fused to metal (PFM). Premolar crowns of a standard design (PFMs and ICAs) or with a modified framework design (PFMm and ICAm) were fabricat...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2008.00608.x
更新日期：2009-04-01 00:00:00
abstract：:The objective of this study was to obtain a geographic world map of scientific production in dentistry by analysing published papers. Articles and reviews in the Dentistry, Oral Surgery & Medicine category published from 1999 to 2003 were accessed through the ISI database. The data were analyzed quantitatively (number...
journal_title：European journal of oral sciences
pub_type： 杂志文章
doi：10.1111/j.1600-0722.2006.00244.x
更新日期：2006-04-01 00:00:00
