Composition of mineralizing incisor enamel in cystic fibrosis transmembrane conductance regulator-deficient mice.

Abstract:

:Formation of crystals in the enamel space releases protons that need to be buffered to sustain mineral accretion. We hypothesized that apical cystic fibrosis transmembrane conductance regulator (CFTR) in maturation ameloblasts transduces chloride into forming enamel as a critical step to secrete bicarbonates. We tested this by determining the calcium, chloride, and fluoride levels in developing enamel of Cftr-null mice by quantitative electron probe microanalysis. Maturation-stage enamel from Cftr-null mice contained less chloride and calcium than did wild-type enamel, was more acidic when stained with pH dyes ex vivo, and formed no fluorescent modulation bands after in vivo injection of the mice with calcein. To acidify the enamel further we exposed Cftr-null mice to fluoride in drinking water to stimulate proton release during formation of hypermineralized lines. In Cftr-deficient mice, fluoride further lowered enamel calcium without further reducing chloride levels. The data support the view that apical CFTR in maturation ameloblasts tranduces chloride into developing enamel as part of the machinery to buffer protons released during mineral accretion.

journal_name

Eur J Oral Sci

authors

Bronckers AL,Lyaruu DM,Guo J,Bijvelds MJ,Bervoets TJ,Zandieh-Doulabi B,Medina JF,Li Z,Zhang Y,DenBesten PK

doi

10.1111/eos.12163

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

9-16

issue

1

eissn

0909-8836

issn

1600-0722

journal_volume

123

pub_type

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