当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Chemically modified nucleic acid aptamers for in vitro selections: evolving evolution.

Chemically modified nucleic acid aptamers for in vitro selections: evolving evolution.

Abstract：

:Combinatorial library selections through the systematic evolution of ligands by exponential enrichment (SELEX) technique identify so-called nucleic acid aptamers that bind with high-affinity and specificity to a wide range of selected molecules. However, the modest chemical functionality of nucleic acids poses some limits on their versatility as binders and catalysts, and, furthermore, the sensitivity of pure RNA- and DNA-based aptamers to nucleases restricts their use as therapeutic and diagnostic agents. Here we review synthetic chemistries for modifying nucleotides that have been developed to enhance the affinity of aptamers for targets and to increase their stability in biological fluids. Implementation of in vitro selections with modified nucleotides promises to be an elegant technique for the creation of ligands with novel physical and chemical properties and is anticipated to have a significant impact on biotechnology, diagnostics and drug development. The current molecular designs and applications of modified nucleotides for in vitro selections are reviewed, along with a discussion of future developments expected to further the utility of this approach in both practical and theoretical terms.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Kusser W

doi

10.1016/s1389-0352(99)00002-1

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

27-38

issue

1

eissn

0168-1656

issn

1873-4863

pii

S1389-0352(99)00002-1

journal_volume

74

pub_type

杂志文章,评审

相关文献

JOURNAL OF BIOTECHNOLOGY文献大全
  • Hydrolysis of softwood by Aspergillus mannanase: role of a carbohydrate-binding module.

    abstract：:Endo beta-1,4-mannanases (beta-mannanases, EC 3.2.1.78), belonging to CAZy GH5 and GH26 families, catalyze the hydrolysis of structurally different mannans. In this study, the mannanase encoding gene of Aspergillus aculeatus VN was expressed in Aspergillus niger D15#26 using pAN 52-4 vector, under the control of PgpdA...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2010.05.012

    authors： Pham TA,Berrin JG,Record E,To KA,Sigoillot JC

    更新日期：2010-08-02 00:00:00

  • Complete genome sequence of Streptomyces fulvissimus.

    abstract：:The complete genome sequence of Streptomyces fulvissimus (DSM 40593), consisting of a linear chromosome with a size of 7.9Mbp, is reported. Preliminary data indicates that the chromosome of S. fulvissimus contains 32 putative gene clusters involved in the biosynthesis of secondary metabolites, two of them showing very...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.08.013

    authors： Myronovskyi M,Tokovenko B,Manderscheid N,Petzke L,Luzhetskyy A

    更新日期：2013-10-10 00:00:00

  • Insertion kinetics of small nucleotides through single walled carbon nanotube.

    abstract：:We report molecular dynamic simulations showing that a DNA molecule constituted by five unique bases can be spontaneously inserted into single walled carbon nanotube (SWCNT) in normal conditions (P, T and water environment) depending on the tube radius value. The van der Waals and electrostatic interactions play a cen...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.12.008

    authors： Clavier A,Kraszewski S,Ramseyer C,Picaud F

    更新日期：2013-03-10 00:00:00

  • Molecular cloning and expression in yeast of caprine prochymosin.

    abstract：:We cloned and characterized a preprochymosin cDNA from the abomasum of milk-fed kid goats. This cDNA contained an open reading frame that predicts a polypeptide of 381 amino acid residues, with a signal peptide and a proenzyme region of 16 and 42 amino acids, respectively. Comparison of the caprine preprochymosin sequ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2004.06.002

    authors： Vega-Hernández MC,Gómez-Coello A,Villar J,Claverie-Martín F

    更新日期：2004-10-19 00:00:00

  • Effect of introducing a disulphide bond between the A and C domains on the activity and stability of Saccharomycopsis fibuligera R64 α-amylase.

    abstract：:Native enzyme and a mutant containing an extra disulphide bridge of recombinant Saccharomycopsis fibuligera R64 α-amylase, designated as Sfamy01 and Sfamy02, respectively, have successfully been overexpressed in the yeast Pichia pastoris KM71H. The purified α-amylase variants demonstrated starch hydrolysis resulting i...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.12.002

    authors： Natalia D,Vidilaseris K,Ismaya WT,Puspasari F,Prawira I,Hasan K,Fibriansah G,Permentier HP,Nurachman Z,Subroto T,Dijkstra BW,Soemitro S

    更新日期：2015-02-10 00:00:00

  • Validation of an optical sensor-based high-throughput bioreactor system for mammalian cell culture.

    abstract：:Cell culture optimization is a labor-intensive process requiring a large number of experiments to be conducted under varying conditions. Here we describe a high-throughput bioreactor system that allows 12 mini stirred-tank bioreactors to be operated simultaneously. All bioreactors are monitored by low-cost minimally i...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.12.009

    authors： Ge X,Hanson M,Shen H,Kostov Y,Brorson KA,Frey DD,Moreira AR,Rao G

    更新日期：2006-04-10 00:00:00

  • Catabolite regulation analysis of Escherichia coli for acetate overflow mechanism and co-consumption of multiple sugars based on systems biology approach using computer simulation.

    abstract：:It is quite important to understand the basic principle embedded in the main metabolism for the interpretation of the fermentation data. For this, it may be useful to understand the regulation mechanism based on systems biology approach. In the present study, we considered the perturbation analysis together with compu...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.06.023

    authors： Matsuoka Y,Shimizu K

    更新日期：2013-10-20 00:00:00

  • Laser reflectance measurement for the online monitoring of Chlorella sorokiniana biomass concentration.

    abstract：:Fast and reliable methods to determine biomass concentration are necessary to facilitate the large scale production of microalgae. A method for the rapid estimation of Chlorella sorokiniana biomass concentration was developed. The method translates the suspension particle size spectrum gathered though laser reflectanc...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2016.12.020

    authors： López Expósito P,Blanco Suárez A,Negro Álvarez C

    更新日期：2017-02-10 00:00:00

  • Lrp of Corynebacterium glutamicum controls expression of the brnFE operon encoding the export system for L-methionine and branched-chain amino acids.

    abstract：:Corynebacterium glutamicum possesses export systems for various amino acids including BrnFE, a two-component export system for L-methionine and the branched-chain amino acids L-valine, L-isoleucine and L-leucine. A gene for a putative transcriptional regulator of the Lrp family is transcribed divergently to the brnFE ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.06.003

    authors： Lange C,Mustafi N,Frunzke J,Kennerknecht N,Wessel M,Bott M,Wendisch VF

    更新日期：2012-04-30 00:00:00

  • Engineering a repression-free catabolite-enhanced expression system for a thermophilic alpha-amylase from Bacillus licheniformis MSG.

    abstract：:Most expression systems used for the over production of many enzymes employ carbon catabolite repressible promoters and hence must use sugar free, rich complex media. Use of expression systems to overcome carbon repression opens an avenue for exploiting cheap carbon sources for the production of recombinant enzymes. A...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.09.016

    authors： Nathan S,Nair M

    更新日期：2013-12-01 00:00:00

  • Evaluation of the GFP signal and its aptitude for novel on-line monitoring strategies of recombinant fermentation processes.

    abstract：:A high number of economically important recombinant proteins are produced in Escherichia coli based host/vector systems. The major obstacle for improving current processes is a lack of appropriate on-line in situ methods for the monitoring of metabolic burden and critical state variables. Here, a pre-evaluation of the...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2003.11.007

    authors： Reischer H,Schotola I,Striedner G,Pötschacher F,Bayer K

    更新日期：2004-03-04 00:00:00

  • A new molecular approach based on qPCR for the quantification of fecal bacteria in contaminated marine sediments.

    abstract：:Harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. Here we first developed new molecular protocols based ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.07.033

    authors： Luna GM,Dell'Anno A,Pietrangeli B,Danovaro R

    更新日期：2012-02-20 00:00:00

  • Thermal stable and oxidation-resistant variant of subtilisin E.

    abstract：:A remarkable thermal stable and oxidation-resistant mutant was obtained using the random mutagenesis PCR technique on the mutant M222A gene of subtilisin E. Sequencing analysis revealed an A was replaced by G at nucleotide 671 of the subtilisin E gene, converting the asparagine codon (AAT) to serine codon (AGT) at pos...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00272-8

    authors： Yang Y,Jiang L,Zhu L,Wu Y,Yang S

    更新日期：2000-08-25 00:00:00

  • DNA vaccination of mice with a plasmid encoding Puumala hantavirus nucleocapsid protein mimics the B-cell response induced by virus infection.

    abstract：:Inoculation of naked DNA has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. To study the humoral immune response induced by DNA vaccination we cloned the entire nucleocapsid protein-encoding sequence of the Puumala hantavirus strain Vranica/Hällnäs into th...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00329-1

    authors： Koletzki D,Schirmbeck R,Lundkvist A,Meisel H,Krüger DH,Ulrich R

    更新日期：2001-11-17 00:00:00

  • Expression and immunogenicity assessment of a plant-made immunogen targeting the cytotoxic T-lymphocyte associated antigen-4: a possible approach for cancer immunotherapy.

    abstract：:Cancer immunotherapy is a promising intervention to fight against this global health problem. In particular targeting immune checkpoints, such as cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and programmed-death protein 1 (PD-1), by specific monoclonal antibodies is a current treatment for many malignances. A ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2021.01.016

    authors： Yiemchavee S,Wong-Arce A,Romero-Maldonado A,Shanmugaraj B,Monsivais-Urenda A,Phoolcharoen W,Rosales-Mendoza S

    更新日期：2021-01-21 00:00:00

  • Mechanisms of ammonia and ammonium ion toxicity in animal cells: transport across cell membranes.

    abstract：:A model for transport of ammonia and ammonium ions across cell membranes is presented. The model suggests that ammonium ions compete with potassium ions for inward transport, over the cytoplasmic membrane, via potassium transport proteins like the Na+/K(+)-ATPase and the Na+K+2Cl(-)-cotransporter. It also explains the...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(93)90148-g

    authors： Martinelle K,Häggström L

    更新日期：1993-09-01 00:00:00

  • Protein response of insect cells to bioreactor environmental stresses.

    abstract：:Protein expression of Spodoptera frugiperda (Sf9) insect cells was characterized upon exposure to environmental stresses typically present in bioreactors including heat shock, oxygen deprivation, shear stress, change of pH, and salinity or ethanol shock. This study fills the void in knowledge as to how bioreactor hydr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.05.009

    authors： Huhtala A,Linko P,Mutharasan R

    更新日期：2005-08-22 00:00:00

  • Simultaneous biosynthesis of (+)-geodin by a lovastatin-producing fungus Aspergillus terreus.

    abstract：:The simultaneous biosynthesis of lovastatin (mevinolinic acid) and (+)-geodin by Aspergillus terreus ATCC 20542 with regard to the medium composition, i.e. the concentrations of carbon and nitrogen source, was described in this paper. A. terreus is a lovastatin producer but the formation of lovastatin was accompanied ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.07.493

    authors： Bizukojc M,Ledakowicz S

    更新日期：2007-12-01 00:00:00

  • Synthesis, characterization of penicillin G capped silver nanoconjugates to combat β-lactamase resistance in infectious microorganism.

    abstract：:In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.12.002

    authors： Ahmed V,Kumar J,Kumar M,Chauhan MB,Vij M,Ganguli M,Chauhan NS

    更新日期：2013-02-20 00:00:00

  • Repeated-batch fermentations of xylose and glucose-xylose mixtures using a respiration-deficient Saccharomyces cerevisiae engineered for xylose metabolism.

    abstract：:Xylose-fermenting Saccharomyces strains are needed for commercialization of ethanol production from lignocellulosic biomass. Engineered Saccharomyces cerevisiae strains expressing XYL1, XYL2 and XYL3 from Pichia stipitis, however, utilize xylose in an oxidative manner, which results in significantly lower ethanol yiel...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2010.09.962

    authors： Kim SR,Lee KS,Choi JH,Ha SJ,Kweon DH,Seo JH,Jin YS

    更新日期：2010-11-01 00:00:00

  • Biocatalytic preparation of acylated derivatives of flavonoid glycosides enhances their antioxidant and antimicrobial activity.

    abstract：:Enzymatic synthesis of acylated derivatives of a monosaccharidic flavonoid chrysoeriol-7-O-beta-D-(3''-E-p-coumaroyl)-glucopyranoside as well as of a disaccharidic flavonoid chrysoeriol-7-[6'''-O-acetyl-beta-D-allosyl-(1-->2)-beta-D-glucopyranoside], isolated from Greek endemic plants, was performed using an immobiliz...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2004.12.002

    authors： Mellou F,Lazari D,Skaltsa H,Tselepis AD,Kolisis FN,Stamatis H

    更新日期：2005-03-30 00:00:00

  • Complete genome sequence of Paenibacillus riograndensis SBR5(T), a Gram-positive diazotrophic rhizobacterium.

    abstract：:Paenibacillus riograndensis is a Gram-positive rhizobacterium which exhibits plant growth promoting activities. It was isolated from the rhizosphere of wheat grown in the state of Rio Grande do Sul, Brazil. Here we announce the complete genome sequence of P. riograndensis strain SBR5(T). The genome of P. riograndensis...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.04.025

    authors： Brito LF,Bach E,Kalinowski J,Rückert C,Wibberg D,Passaglia LM,Wendisch VF

    更新日期：2015-08-10 00:00:00

  • Investigation of relationship between lipid and Monascus pigment accumulation by extractive fermentation.

    abstract：:Fermented Monascus pigments have been utilized as traditional Chinese medicine and food colorant for thousands of years. Under the limited nitrogen concentration and/or low initial pH 2.5 conditions, it was observed that production of intracellular pigments and accumulation of microbial lipids (high content reaching t...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.08.019

    authors： Wang B,Zhang X,Wu Z,Wang Z

    更新日期：2015-10-20 00:00:00

  • Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris.

    abstract：:The methylotropic yeast Pichia pastoris has demonstrated its suitability for large-scale production of recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins when compared with Escherichia coli. In this work, P. pastoris has bee...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2016.04.024

    authors： de la Cruz S,Alcocer M,Madrid R,García A,Martín R,González I,García T

    更新日期：2016-06-10 00:00:00

  • 28S rRNA is inducibly pseudouridylated by the mTOR pathway translational control in CHO cell cultures.

    abstract：:The mTOR pathway is a conserved master regulator of translational activity that influences the fate of industrially relevant CHO cell cultures, yet its molecular mechanisms remain unclear. Interestingly, rapamycin specific inhibition of the mTOR pathway in CHO cells was found to down-regulate the small nucleolar RNA U...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.01.024

    authors： Courtes FC,Gu C,Wong NS,Dedon PC,Yap MG,Lee DY

    更新日期：2014-03-20 00:00:00

  • Serum-free production of rVSV-ZEBOV in Vero cells: Microcarrier bioreactor versus scale-X™ hydro fixed-bed.

    abstract：:Ebola virus disease outbreaks have repeatedly occurred on the African continent over the last decades, with more serious outbreaks in recent years. Being highly transmissible and associated to high fatality rates, it constitutes a serious threat to public health. Vaccination, however, may allow for efficient control o...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2020.01.015

    authors： Kiesslich S,Vila-Chã Losa JP,Gélinas JF,Kamen AA

    更新日期：2020-02-20 00:00:00

  • Production processes of recombinant IL-1 beta from Bacillus subtilis: comparison between intracellular and exocellular expression.

    abstract：:The human IL-1 beta coding sequence derived from a cDNA library was inserted into two different plasmid expression vectors, pSM214 and pSM308, which promote the synthesis of recombinant products intracellularly and exocellularly, respectively. The hybrid constructs pSM261 and pSM320 were obtained. Bacillus subtilis SM...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(91)90246-r

    authors： Bellini AV,Galli G,Fascetti E,Frascotti G,Branduzzi P,Lucchese G,Grandi G

    更新日期：1991-05-01 00:00:00

  • The C-terminal region controls correct folding of genus Trametes pyranose 2-oxidases.

    abstract：:The pyranose 2-oxidases from Trametes ochracea and Trametes pubescens share markedly similar amino acid sequences with identity of 93.4%. When expressed from the recombinant plasmids based on the same vector in the Escherichia coli host strain BL21(DE3) at higher growth temperatures, they differ strikingly in the form...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.04.023

    authors： Maresová H,Palyzová A,Kyslík P

    更新日期：2007-06-30 00:00:00

  • D-xylose detection in Escherichia coli by a xylose binding protein-dependent response.

    abstract：:A gene circuit for the controlled expression of a marker gene and for the assay of xylose concentration in Escherichia coli has been designed and tested. The xylF coding sequence for the xylose binding protein (XBP) was cloned in pT7T318U downstream from the promoter for xylanase A from B. subtilis (Pbsu), together wi...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.10.019

    authors： Ribeiro LF,Bressan F,Furtado GP,Meireles F,Ward RJ

    更新日期：2013-12-01 00:00:00

  • Targeting N-acyl-homoserine-lactones to mitigate membrane biofouling based on quorum sensing using a biofouling reducer.

    abstract：:Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membrane biofouling. Thi...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.06.029

    authors： Siddiqui MF,Sakinah M,Singh L,Zularisam AW

    更新日期：2012-10-31 00:00:00