Chemically modified nucleic acid aptamers for in vitro selections: evolving evolution.


:Combinatorial library selections through the systematic evolution of ligands by exponential enrichment (SELEX) technique identify so-called nucleic acid aptamers that bind with high-affinity and specificity to a wide range of selected molecules. However, the modest chemical functionality of nucleic acids poses some limits on their versatility as binders and catalysts, and, furthermore, the sensitivity of pure RNA- and DNA-based aptamers to nucleases restricts their use as therapeutic and diagnostic agents. Here we review synthetic chemistries for modifying nucleotides that have been developed to enhance the affinity of aptamers for targets and to increase their stability in biological fluids. Implementation of in vitro selections with modified nucleotides promises to be an elegant technique for the creation of ligands with novel physical and chemical properties and is anticipated to have a significant impact on biotechnology, diagnostics and drug development. The current molecular designs and applications of modified nucleotides for in vitro selections are reviewed, along with a discussion of future developments expected to further the utility of this approach in both practical and theoretical terms.


J Biotechnol


Journal of biotechnology


Kusser W




Has Abstract


2000-03-01 00:00:00














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    authors: Pham TA,Berrin JG,Record E,To KA,Sigoillot JC

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  • Complete genome sequence of Streptomyces fulvissimus.

    abstract::The complete genome sequence of Streptomyces fulvissimus (DSM 40593), consisting of a linear chromosome with a size of 7.9Mbp, is reported. Preliminary data indicates that the chromosome of S. fulvissimus contains 32 putative gene clusters involved in the biosynthesis of secondary metabolites, two of them showing very...

    journal_title:Journal of biotechnology

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    authors: Myronovskyi M,Tokovenko B,Manderscheid N,Petzke L,Luzhetskyy A

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  • Insertion kinetics of small nucleotides through single walled carbon nanotube.

    abstract::We report molecular dynamic simulations showing that a DNA molecule constituted by five unique bases can be spontaneously inserted into single walled carbon nanotube (SWCNT) in normal conditions (P, T and water environment) depending on the tube radius value. The van der Waals and electrostatic interactions play a cen...

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    authors: Clavier A,Kraszewski S,Ramseyer C,Picaud F

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  • Molecular cloning and expression in yeast of caprine prochymosin.

    abstract::We cloned and characterized a preprochymosin cDNA from the abomasum of milk-fed kid goats. This cDNA contained an open reading frame that predicts a polypeptide of 381 amino acid residues, with a signal peptide and a proenzyme region of 16 and 42 amino acids, respectively. Comparison of the caprine preprochymosin sequ...

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  • Effect of introducing a disulphide bond between the A and C domains on the activity and stability of Saccharomycopsis fibuligera R64 α-amylase.

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    journal_title:Journal of biotechnology

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    authors: Natalia D,Vidilaseris K,Ismaya WT,Puspasari F,Prawira I,Hasan K,Fibriansah G,Permentier HP,Nurachman Z,Subroto T,Dijkstra BW,Soemitro S

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    authors: López Expósito P,Blanco Suárez A,Negro Álvarez C

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    journal_title:Journal of biotechnology

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    authors: Lange C,Mustafi N,Frunzke J,Kennerknecht N,Wessel M,Bott M,Wendisch VF

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    pub_type: 杂志文章


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    authors: Bellini AV,Galli G,Fascetti E,Frascotti G,Branduzzi P,Lucchese G,Grandi G

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    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Maresová H,Palyzová A,Kyslík P

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    pub_type: 杂志文章


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