Complete genome sequence of Streptomyces fulvissimus.

abstract：:A computer system solution for integration of a distributed bioreactor monitoring and control instrumentation on the laboratory scale is described. Bioreactors equipped with on-line analyzers for mass spectrometry, near-infrared spectroscopy, electrochemical probes and multi-array gas sensors and their respective soft...

journal_title：Journal of biotechnology

authors： Cimander C,Bachinger T,Mandenius CF

更新日期：2003-08-15 00:00:00

abstract：:Enantiomers of 3-hydroxybutyric acid (3-HB) can be used as the chiral precursors for the production of various optically active fine chemicals, including drugs, perfumes, and pheromones. In this study, Escherichia coli was engineered to produce (S)-3-HB from glucose through the inverted reactions of the native aerobic...

journal_title：Journal of biotechnology

authors： Gulevich AY,Skorokhodova AY,Sukhozhenko AV,Debabov VG

更新日期：2017-02-20 00:00:00

abstract：:Medium composition and cultivation conditions were constructed for the optimum production of leupeptin by Streptomyces exfoliatus SMF13. The production of leupeptin was related to mycelial growth, being optimum in the cultivation with glucose-excess, phosphate-limited, and casamino acids medium. However, leupeptin-ina...

journal_title：Journal of biotechnology

authors： Kim IS,Lee KJ

更新日期：1995-08-15 00:00:00

abstract：:The Bacillus subtilis endoxylanase XynA (BSXY) is frequently used to improve the functionality of arabinoxylan-containing material in cereal based industries. The presence of endogenous Triticum aestivum xylanase inhibitors (TAXI-I and TAXI-II) in wheat is a real concern as they have a direct negative impact on the ef...

journal_title：Journal of biotechnology

authors： Bourgois TM,Nguyen DV,Sansen S,Rombouts S,Beliën T,Fierens K,Raedschelders G,Rabijns A,Courtin CM,Delcour JA,Van Campenhout S,Volckaert G

更新日期：2007-05-31 00:00:00

abstract：:We have carried out the adaptation of BHK-21 cells to two serum free (Ex Cell 520 and HyQ PF CHO) and three animal protein free media: Ex Cell 302, HyQ PF CHO MPS and Rencyte BHK. After a direct switch or a gradual adaptation, we have achieved BHK-21 cells growth in the following media: HyQ PF CHO, HyQ PF CHO MPS, Ren...

journal_title：Journal of biotechnology

authors： Kallel H,Jouini A,Majoul S,Rourou S

更新日期：2002-05-23 00:00:00

abstract：:This study evaluates the suitability of flow cytometry with the fluorochrome BCECF for measuring the intracellular pH (pHi) of cultured cells, and monitors the changes in pHi in murine hybridoma in batch culture and chick embryo fibroblast in monolayer culture (5th passage). The technique produced highly reproducible,...

journal_title：Journal of biotechnology

authors： Franck P,Petitipain N,Cherlet M,Dardennes M,Maachi F,Schutz B,Poisson L,Nabet P

更新日期：1996-05-15 00:00:00

abstract：:Glucose oxidase (GOD) was immobilized on cellulose acetate-polymethylmethacrylate (CA-PMMA) membrane. The immobilized GOD showed better performance as compared to the free enzyme in terms of thermal stability retaining 46% of the original activity at 70 degrees C where the original activity corresponded to that obtain...

journal_title：Journal of biotechnology

authors： Rauf S,Ihsan A,Akhtar K,Ghauri MA,Rahman M,Anwar MA,Khalid AM

更新日期：2006-02-10 00:00:00

abstract：:siRNA based therapeutics have become the next frontier in molecular medicine. Though exosomes emerge as a promising drug delivery vehicle for siRNAs, significant hurdle remains in finding safe and effective loading methods. Traditional methods of loading exogenous siRNAs in exosomes are marked by certain limitations l...

journal_title：Journal of biotechnology

authors： Shandilya S,Rani P,Onteru SK,Singh D

更新日期：2020-07-20 00:00:00

abstract：:We previously reported that a recombinant strain, SB412(pLGM304), was constructed from acidomycin-resistant mutants of Serratia marcescens and produced 200 mg of d-biotin per liter of a medium containing sucrose and urea (Sakurai et al., 1993a, b). In the present work, we intended to improve the d-biotin production. B...

journal_title：Journal of biotechnology

authors： Sakurai N,Imai Y,Masuda M,Komatsubara S,Tosa T

更新日期：1994-07-29 00:00:00

abstract：:Based on the prototype experiment of treating herb wastewater by Up flow Anaerobic Sludge Bed and Anaerobic Filter reactor (UASBAF), an artificial neural network (ANN) model which adopts a back propagation algorithm with momentum and adaptive learning rate was established. And the effect of each parameter to the perfo...

journal_title：Journal of biotechnology

authors： Shi Y,Zhao XT,Zhang YM,Ren NQ

更新日期：2009-10-12 00:00:00

abstract：:A new, chemically defined medium providing dispersed growth and high biomass formation and a method for quantitative extraction of intracellular metabolites was used to investigate the cellular response of Streptomyces coelicolor A3(2) during growth and upon changes in nutrient utilization. Fast changes of the glucose...

journal_title：Journal of biotechnology

authors： Mira de Orduña R,Theobald U

更新日期：2000-02-17 00:00:00

abstract：:Three thermostable lactose-hydrolases, namely, two beta-glycosidases (bglA and bglB) and one beta-galactosidase (bgaA) genes were cloned from the genomic library of Thermus sp. IB-21. The bglA, bglB, and bgaA consisted of 1311 bp (436 amino acid residues), 1296 bp (431 aa), and 1938 bp (645 aa) of nucleotides with pre...

journal_title：Journal of biotechnology

authors： Kang SK,Cho KK,Ahn JK,Bok JD,Kang SH,Woo JH,Lee HG,You SK,Choi YJ

更新日期：2005-04-06 00:00:00

abstract：:Indigo is an insoluble blue dye, which generates serious pollution in its production process. Increasing focus has come to the biosynthesis of indigo that are more environment-preserved and high-efficient. Hence, this study was designed to explore the specific role of various deep eutectic solvents (DESs) on cytochrom...

journal_title：Journal of biotechnology

authors： Sun H,Xin R,Qu D,Yao F

更新日期：2020-11-10 00:00:00

abstract：:Corynebacterium glutamicum is an industrially important producer of amino acids and an emerging model system for the Corynebacterineae. The glxR gene of C. glutamicum ATCC 13032 encodes a DNA binding transcription factor of the Crp-Fnr protein family. Available data indicated a prominent role of GlxR in the transcript...

journal_title：Journal of biotechnology

authors： Kohl TA,Tauch A

更新日期：2009-09-25 00:00:00

abstract：:Vertebrate prostaglandin H synthases (PGHSs) are membrane-bound disulphide-containing hemoglycoproteins. Therefore, eukaryotic expression systems are required for the production of recombinant PGHSs. Recently we announced the expression of human PGHS-2 (hPGHS-2) in the yeast Pichia pastoris. Here we report improved pr...

journal_title：Journal of biotechnology

authors： Kukk K,Samel N

更新日期：2016-08-10 00:00:00

abstract：:Semi-synthetic β-lactam antibiotics (SSBAs) are one of the most important antibiotic families in the world market. Their enzymatic synthesis can be catalyzed by penicillin G acylases (PGAs). In this study, to improve enzymatic synthesis of ampicillin, site-saturating mutagenesis was performed on three conserved amino ...

journal_title：Journal of biotechnology

authors： Deng S,Su E,Ma X,Yang S,Wei D

更新日期：2015-04-10 00:00:00

abstract：:The expression of recombinant yeast pyruvate carboxylase (PYC) in animal cell lines was shown in previous studies to reduce significantly the formation of waste metabolites, although it has translated into mixed results in terms of improved cellular growth and productivity. In this work, we demonstrate that the unique...

journal_title：Journal of biotechnology

authors： Vallée C,Durocher Y,Henry O

更新日期：2014-01-01 00:00:00

abstract：:The adsorption behaviors of two glucanases, TvEG and TrCel7A, on three lignins were investigated. Three lignins were isolated from raw aspen and its pretreated solid residue. The isolated lignins were labeled as Asp-MWL, DA-MWL (pretreated by dilute acid), and GL-MWL (pretreated by green liquor), respectively. The sur...

journal_title：Journal of biotechnology

authors： Zhang Y,Jiang X,Wan S,Wu W,Wu S,Jin Y

更新日期：2020-11-10 00:00:00

abstract：:R-lactide, a pivotal monomer for the production of poly (D-lactic acid) (PDLA) or stereocomplex poly (lactic acid) (PLA) was synthesized from alkyl (R)-lactate through a lipase-catalyzed reaction without racemization. From among several types of lipase, only lipase B from Candida antarctica (Novozym 435; CAL-B) was ef...

journal_title：Journal of biotechnology

authors： Jeon BW,Lee J,Kim HS,Cho DH,Lee H,Chang R,Kim YH

更新日期：2013-10-20 00:00:00

abstract：:In n-hexadecane fermentation by Pseudomonas aeruginosa, the production of rhamnolipids (biosurfactants) caused by nitrogen source limitation was observed during the stationary phase. The rhamnolipids caused severe foaming in the process, particularly at higher pH (ca. > 6.8). To reduce the foaming, several runs were m...

journal_title：Journal of biotechnology

authors： Wu J,Ju LK

更新日期：1997-01-03 00:00:00

abstract：:Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. Here we described a novel approach to construct plant amiRNA expression vectors with seamless enzyme-free cloning (SEFC) and mating-assisted genetically integrated cloning ...

journal_title：Journal of biotechnology

authors： Yan H,Deng X,Cao Y,Huang J,Ma L,Zhao B

更新日期：2011-01-10 00:00:00

abstract：:The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). T...

journal_title：Journal of biotechnology

authors： Schenk JA,Fettke J,Lenz C,Albers K,Mallwitz F,Gajovic-Eichelmann N,Ehrentreich-Förster E,Kusch E,Sellrie F

更新日期：2012-03-31 00:00:00

abstract：:Vigna radiata (mung bean) is an important crop plant and is a major protein source in developing countries. Mung bean 8S globulins constitute nearly 90% of total seed storage protein and consist of three subunits designated as 8SGα, 8SGα' and 8SGβ. The 5'-flanking sequences of 8SGα' has been reported to confer high ex...

journal_title：Journal of biotechnology

authors： Chen MX,Zheng SX,Yang YN,Xu C,Liu JS,Yang WD,Chye ML,Li HY

更新日期：2014-03-20 00:00:00

abstract：:The global occurrence of prostate cancer with a range of patient outcome has prompted various investigators to explore novel molecular biomarkers that can precisely detect and track this type of cancer severity. Several studies suggest that micro-RNAs have emerged to act as a new largely unexplored class of biomarkers...

journal_title：Journal of biotechnology

authors： Wani S,Kaul D,Mavuduru RS,Kakkar N,Bhatia A

更新日期：2017-10-10 00:00:00

abstract：:The kinetics of microbial growth and the biodegradation of methanol and toluene in (a) biofilters (BFs), and (b) biotrickling filters (BTFs), packed with inert materials, has been studied and analyzed. The specific growth rate, mu, for the treatment of methanol was 0.037h(-1) for a wide range of operating conditions. ...

journal_title：Journal of biotechnology

authors： Avalos Ramirez A,Bénard S,Giroir-Fendler A,Jones JP,Heitz M

更新日期：2008-11-25 00:00:00

abstract：:Expression of cloned PhiX174 gene E in bacteria results in lysis of bacteria. It is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of Gram-negative bacteria. The resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic conten...

journal_title：Journal of biotechnology

authors： Szostak MP,Hensel A,Eko FO,Klein R,Auer T,Mader H,Haslberger A,Bunka S,Wanner G,Lubitz W

更新日期：1996-01-26 00:00:00

abstract：:Palladium (Pd) is commonly used as a catalyst for automobiles and electronic devices, and a reliable source of Pd is required for continued commercial applications. Biomineralization has attracted attention as an inexpensive and eco-friendly recycling approach for a continued supply of Pd. Escherichia coli is one of t...

journal_title：Journal of biotechnology

authors： Matsumoto T,Kamino M,Yamada R,Konishi Y,Ogino H

更新日期：2020-12-20 00:00:00

abstract：:This work aims at investigating the effect of the nanoemulsion delivery systems on the antimicrobial activity of different essential oil components. Carvacrol, limonene and cinnamaldehyde were encapsulated in the sunflower oil droplets of nanoemulsions prepared by high pressure homogenization and stabilized by differe...

journal_title：Journal of biotechnology

authors： Donsì F,Annunziata M,Vincensi M,Ferrari G

更新日期：2012-06-30 00:00:00

abstract：:Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrog...

journal_title：Journal of biotechnology

authors： Pedrosa FO,Benelli EM,Yates MG,Wassem R,Monteiro RA,Klassen G,Steffens MB,Souza EM,Chubatsu LS,Rigo LU

更新日期：2001-10-04 00:00:00

abstract：:The successful transfer of human embryonic stem cell (hESC) technology and cellular products into clinical and industrial applications needs to address issues of automation, standardization and the generation of relevant cell numbers of high quality. In this study, we combined microcarrier technology and controlled st...

journal_title：Journal of biotechnology

authors： Serra M,Brito C,Sousa MF,Jensen J,Tostões R,Clemente J,Strehl R,Hyllner J,Carrondo MJ,Alves PM

更新日期：2010-08-02 00:00:00