当前位置： SCI文献检索 > JOURNAL OF DERMATOLOGICAL SCIENCE期刊下所有文献 > gp100 mRNA is more sensitive than tyrosinase mRNA for RT-PCR amplification to detect circulating melanoma cells in peripheral blood of melanoma patients.

gp100 mRNA is more sensitive than tyrosinase mRNA for RT-PCR amplification to detect circulating melanoma cells in peripheral blood of melanoma patients.

Abstract：

:Two different melanocyte-specific mRNAs are studied as markers for circulating melanoma cells in vitro using the human melanoma cell line G361 and in vivo using blood samples from Japanese melanoma patients at different clinical stages. These mRNAs encode tyrosinase, the most essential enzyme for melanin synthesis, and gp100, a melanosomal matrix glycoprotein recognized by monoclonal antibody HMB-45. We used reverse-transcription polymerase chain reaction (RT-PCR) to detect tyrosinase mRNA and gp100 mRNA in peripheral blood. Since melanocytes would not normally be present in peripheral blood, the detection of those transcripts should indicate the presence of circulating melanoma cells. RT-PCR detection of these two mRNAs was highly sensitive and specific. Our in vitro study showed that as few as 10 melanoma cells in 0.125 ml normal blood could be detected. In in vivo study, 130 blood samples from 55 melanoma patients gave positive and variably sensitive results, whereas no samples from healthy controls or patients with other cancers gave positive results. Tyrosinase mRNA was not detected in any of the melanoma patients. gp100 mRNA was detected in 12 of 55 melanoma patients, in none of five stage I patients (0%), in four of 26 stage II patients (15.4%), in one of six stage III patients (16. 7%) and in seven of 18 stage IV patients (38.9%). Thus gp100 mRNA is a more sensitive marker for detecting circulating melanoma cells compared with tyrosinase mRNA.

journal_name

J Dermatol Sci

journal_title

Journal of dermatological science

authors

Tsukamoto K,Ueda M,Hirata S,Osada A,Kitamura R,Takahashi T,Ichihashi M,Shimada S

doi

10.1016/s0923-1811(99)00098-5

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

126-31

issue

2

eissn

0923-1811

issn

1873-569X

pii

S0923-1811(99)00098-5

journal_volume

23

pub_type

杂志文章

相关文献

JOURNAL OF DERMATOLOGICAL SCIENCE文献大全
  • Malignant blue nevus: a report of eight cases.

    abstract：:Malignant blue nevus is uncommon compared to its benign counterpart and is regarded as a rare form of malignant melanoma. We report the clinical and histological findings in eight cases. Histologically, all eight specimens showed no epidermal involvement and had contained within or were adjacent to portions of blue ne...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/0923-1811(92)90018-7

    authors： Mehregan DA,Gibson LE,Mehregan AH

    更新日期：1992-11-01 00:00:00

  • Role of YAP-related T cell imbalance and epidermal keratinocyte dysfunction in the pathogenesis of atopic dermatitis.

    abstract：BACKGROUND:Atopic dermatitis (AD) is characterized by impaired skin barrier function and immune system dysfunction. The expression and role of Yes-associated protein (YAP) in AD are unclear. OBJECTIVE:To characterize the role of the YAP in T cell imbalance and epidermal keratinocyte dysfunction in the pathogenesis of ...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2020.12.004

    authors： Jia J,Mo X,Yan F,Liu J,Ye S,Zhang Y,Lin Y,Li H,Chen D

    更新日期：2020-12-18 00:00:00

  • Identification of mutations in the prostaglandin transporter gene SLCO2A1 and its phenotype-genotype correlation in Japanese patients with pachydermoperiostosis.

    abstract：BACKGROUND:Pachydermoperiostosis (PDP) is a rare genetic disorder characterized by 3 major symptoms: pachydermia including cutis verticis gyrata (CVG), periostosis, and finger clubbing. Recently, a homozygous mutation in the gene HPGD, which encodes 15-hydroxyprostaglandin dehydrogenase (15-PGDH), was found to be assoc...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2012.07.008

    authors： Sasaki T,Niizeki H,Shimizu A,Shiohama A,Hirakiyama A,Okuyama T,Seki A,Kabashima K,Otsuka A,Ishiko A,Tanese K,Miyakawa S,Sakabe J,Kuwahara M,Amagai M,Okano H,Suematsu M,Kudoh J

    更新日期：2012-10-01 00:00:00

  • There is no influence of a temperature rise on in vivo adsorption of UV filters into the stratum corneum.

    abstract：:Temperature influences the stratum corneum adsorption of several topically applied compounds. This study was designed to evaluate the influence of the temperature on the stratum corneum adsorption of 3 UV filters. The UV filters were solubilized in two vehicles, an emulsion gel and petroleum jelly and applied at respe...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/s0923-1811(01)00100-1

    authors： Clarys P,Gabard B,Lambrecht R,Barel A,Bieli E,Lüdi S

    更新日期：2001-10-01 00:00:00

  • Tranexamic acid inhibits melanogenesis by activating the autophagy system in cultured melanoma cells.

    abstract：BACKGROUND:As interest in skin beauty increases, the development of new skin whitening agents has attracted substantial attention; however, the action mechanism of the agents developed so far remains largely unknown. Tranexamic acid (TXA) is commonly being used to reduce melanin synthesis in patients with melasma and a...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2017.05.019

    authors： Cho YH,Park JE,Lim DS,Lee JS

    更新日期：2017-10-01 00:00:00

  • Update on contact dermatitis 2007: Medical pearls from the annual meeting of the British Contact Dermatitis Society at the annual meeting of the British Association of Dermatologists.

    abstract：:Contact dermatitis is an integral part of dermatology. Many patients affected by contact dermatitis suffer significant morbidity and require prompt diagnosis and treatment. Here we give an overview of contact dermatitis related presentations made during the British Contact Dermatitis Society at 87th annual meeting of ...

    journal_title：Journal of dermatological science

    pub_type：

    doi：10.1016/j.jdermsci.2008.02.002

    authors： Alexandroff AB,Gawkrodger DJ

    更新日期：2008-09-01 00:00:00

  • Transcriptomic analysis of human dermal fibroblast cells reveals potential mechanisms underlying the protective effects of visible red light against damage from ultraviolet B light.

    abstract：BACKGROUND:Ultraviolet B (UVB) radiation is a major cause of skin photodamage, including the damage associated with photodermatoses, aging, and cancer. Although many studies have shown that red light has photoprotective effects on skin, the mechanisms underlying these effects are still poorly understood. OBJECTIVE:The...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2019.03.003

    authors： Kim HS,Kim YJ,Kim SJ,Kang DS,Lee TR,Shin DW,Kim HJ,Seo YR

    更新日期：2019-05-01 00:00:00

  • Role of the aryl hydrocarbon receptor signaling pathway in promoting mitochondrial biogenesis against oxidative damage in human melanocytes.

    abstract：BACKGROUND:Reactive oxygen species (ROS)-induced mitochondrial damage aggravates oxidative stress and activates mitochondrial apoptosis pathway to mediate melanocyte death. However, the repair mechanisms underlying damaged mitochondria of melanocytes remain unclear. Accumulative evidence has revealed that the aryl hydr...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2019.09.001

    authors： Wang X,Li S,Liu L,Jian Z,Cui T,Yang Y,Guo S,Yi X,Wang G,Li C,Gao T,Li K

    更新日期：2019-10-01 00:00:00

  • Prognostic factors in 105 Japanese cases of mycosis fungoides and Sézary syndrome: clusterin expression as a novel prognostic factor.

    abstract：BACKGROUND:The analysis of prognostic factors in Japanese cases of cutaneous T-cell lymphoma (CTCL) is scarce. Clusterin is a ubiquitous 80kDa heterodimeric glycoprotein expressed on tumor cells of systemic and primary cutaneous anaplastic large cell lymphoma. The expression of clusterin in mycosis fungoides (MF) and S...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2013.04.020

    authors： Tobisawa S,Honma M,Ishida-Yamamoto A,Saijo Y,Iizuka H

    更新日期：2013-09-01 00:00:00

  • Gene expression in canine atopic dermatitis and correlation with clinical severity scores.

    abstract：BACKGROUND:Canine atopic dermatitis (cAD) is a common condition in dogs that may be a naturally occurring model for human atopic dermatitis (hAD). Despite this, comparative research is limited, particularly into the genetic background of cAD. OBJECTIVES:1. Measure candidate gene expression in cAD skin using quantitati...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2009.03.005

    authors： Wood SH,Clements DN,Ollier WE,Nuttall T,McEwan NA,Carter SD

    更新日期：2009-07-01 00:00:00

  • Detection of interferon-gamma mRNA in psoriatic epidermis by polymerase chain reaction.

    abstract：:Psoriatic skin lesions contain HLA-DR positive T lymphocytes, and other activation antigens, which suggest that the T cells may be producing lymphokines. Gamma interferon is produced by activated T cells, and its presence in psoriasis has been inferred by the lesional keratinocyte expression of 3 gamma interferon-indu...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/0923-1811(91)90019-t

    authors： Barker JN,Karabin GD,Stoof TJ,Sarma VJ,Dixit VM,Nickoloff BJ

    更新日期：1991-03-01 00:00:00

  • Complementary effect of hydroquinone and retinoic acid on corneocyte desquamation with their combination use.

    abstract：BACKGROUND:Retinoic acid (RA) enhances skin-lightening capabilities of hydroquinone (HQ), at least in part, by facilitating desquamation which leads to increase penetration of HQ. The desquamation also affects skin irritation levels. The mechanism of RA-induced desquamation, however, has not been completely explored an...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2017.03.023

    authors： Cheong KA,Lee TR,Lee AY

    更新日期：2017-08-01 00:00:00

  • Proteolytic cleavage of Livin (ML-IAP) in apoptotic melanoma cells potentially mediated by a non-canonical caspase.

    abstract：BACKGROUND:Several inhibitor of apoptosis proteins (IAPs) are cleaved during apoptosis. Studies of the melanoma-associated IAP (ML-IAP) Livin, using recombinant molecules, have implicated both caspases 3/7 and the serine protease Omi/HtrA2 in its proteolytic cleavage. OBJECTIVE:To characterize the apoptotic cleavage o...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2006.05.007

    authors： Yan H,Brouha B,Liu T,Raj D,Biddle D,Lee R,Grossman D

    更新日期：2006-09-01 00:00:00

  • Neither cyclosporin A nor FK506 affects adenylate cyclase responses of fetal rat keratinizing epidermal cells (FRSK cells) at concentrations that inhibit thymidine incorporation.

    abstract：:It has been reported that various anti-psoriatic agents augment the beta-adrenergic adenylate cyclase response that is defective in the psoriatic hyperproliferative epidermis. Recent evidence indicates that cyclosporin A (CsA) and FK506 show beneficial effects on psoriasis. Using fetal rat keratinizing epidermal cells...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/0923-1811(93)90044-p

    authors： Takahashi H,Iizuka H

    更新日期：1993-12-01 00:00:00

  • Retinoid-induced beta-adrenergic inhibitory effect associated with increased thymidine incorporation of fetal rat keratinizing epidermal cells (FRSK cells).

    abstract：:Using fetal rat keratinizing epidermal cells (FRSK cells) we investigated the effects of retinoids on the cyclic AMP system. The beta-adrenergic adenylate cyclase response was significantly decreased by the treatment of cells with 1 x 10(-6) M Ro 10-1670. The effect was observed by 12 h and remained for at least 48 h....

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/0923-1811(93)90080-9

    authors： Takahashi H,Iizuka H

    更新日期：1993-04-01 00:00:00

  • A lamellar body mimetic system for the treatment of oxazolone-induced atopic dermatitis in hairless mice.

    abstract：BACKGROUND:Atopic dermatitis is a common skin disease characterized by a Th2 cell-dominant inflammatory infiltrate, elevated serum IgE levels and impaired epidermal barrier function. It is associated to abnormal epidermal lamellar body secretion, producing alteration in lipid composition and extracellular lamellar memb...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2018.01.010

    authors： Moner V,Fernández E,Calpena AC,Garcia-Herrera A,Cócera M,López O

    更新日期：2018-05-01 00:00:00

  • The role of classical and alternative macrophages in the immunopathogenesis of herpes simplex virus-induced inflammation in a mouse model.

    abstract：BACKGROUND:The exact mechanism of the inflammatory changes occurring during the development of Behçet's disease (BD) remains unclear. OBJECTIVE:We investigated the role of classical (M1) and alternative (M2) activation of macrophages in a herpes simplex virus (HSV)-induced BD mouse model. METHODS:The classical vs. al...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2013.11.001

    authors： Anower AK,Shim JA,Choi B,Kwon HJ,Sohn S

    更新日期：2014-03-01 00:00:00

  • Time course of changes in antioxidant enzymes in human skin fibroblasts after UVA irradiation.

    abstract：:Examining damage (inactivation) of antioxidant enzymes in the cells and the pattern of recovery after a single UV exposure might be a useful method for analyzing the mechanisms of chronic UV irradiation, because chronic UV irradiation means repeated single exposures. We irradiated human skin fibroblasts with a single ...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/s0923-1811(96)00578-6

    authors： Shindo Y,Hashimoto T

    更新日期：1997-03-01 00:00:00

  • rAAV2-mediated restoration of LEKTI in LEKTI-deficient cells from Netherton patients.

    abstract：BACKGROUND:Netherton syndrome (NS, MIM 256500) is a potential live threatening autosomal-recessive skin disorder clinically characterized by the trias of congenital erythroderma, hair shaft anomalies and atopic diathesis. It is caused by mutations in the gene SPINK5 resulting in a deficiency of its processed protein na...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2010.12.004

    authors： Roedl D,Oji V,Buters JT,Behrendt H,Braun-Falco M

    更新日期：2011-03-01 00:00:00

  • The retinoic acid-induced up-regulation of insulin-like growth factor 1 and 2 is associated with prolidase-dependent collagen synthesis in UVA-irradiated human dermal equivalents.

    abstract：BACKGROUND:Ultraviolet (UV) A irradiation causes the degeneration of extracellular matrix in the skin dermis, mainly due to disrupted collagen homeostasis, resulting in the photo-aging of human skin. All-trans retinoic acid (ATRA) improves photo-aged human skin in vivo. OBJECTIVES:Although the effects of ATRA on colla...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2011.12.008

    authors： Shim JH,Shin DW,Lee TR,Kang HH,Jin SH,Noh M

    更新日期：2012-04-01 00:00:00

  • Alterations of immune functions in barrier disrupted skin by UVB irradiation.

    abstract：BACKGROUND:While immunologic events elicited by acute barrier disruption or UVB irradiation have been studied in detail, the biological sequel of multiple insults to the skin is not well understood. OBJECTIVE:Since the skin would receive a variety of simultaneous stimuli in daily life, we tested the effects of sequent...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/s0923-1811(03)00177-4

    authors： Ito T,Seo N,Yagita H,Tsujimura K,Takigawa M,Tokura Y

    更新日期：2003-12-01 00:00:00

  • Production of epidermal sheets in a serum free culture system: a further appraisal of the role of extracellular calcium.

    abstract：:Rhenwald and Green's technique is currently the standard method for growing stratifying epidermal cell cultures. The serum free system developed in Ham's laboratory (MCDB 153) was designed to grow keratinocyte monolayers in clonogenic conditions. Our aim was to optimize conditions in serum-free MCDB 153 for culturing ...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/0923-1811(92)90044-c

    authors： Boisseau AM,Donatien P,Surlève-Bazeille JE,Amédée J,Harmand MF,Bézian JH,Maleville J,Taieb A

    更新日期：1992-03-01 00:00:00

  • Vascular endothelial growth factor (VEGF) in the pathogenesis of psoriasis--a possible target for novel therapies?

    abstract：:Angiogenesis is defined as the formation of new capillaries from pre-existing blood vessels. The process of angiogenesis is tightly regulated by a balance between pro- and anti-angiogenic factors. Vascular endothelial growth factor (VEGF) is a pro-angiogenic factor and several anti-VEGF therapies are used in the treat...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章,评审

    doi：10.1016/j.jdermsci.2010.03.023

    authors： Canavese M,Altruda F,Ruzicka T,Schauber J

    更新日期：2010-06-01 00:00:00

  • Assessment of in vitro percutaneous absorption of glycolic acid through human skin sections using a flow-through diffusion cell system.

    abstract：UNLABELLED:The percutaneous absorption studies were performed using a flow-through diffusion cell system with skin specimens from 24 healthy women to assess the penetration of glycolic acid (GA). Percentages of GA, based on 14C-labelled activity, found in the skin after application of 4% GA at pH 2.0 or pH 3.8, after 2...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/s0923-1811(98)00039-5

    authors： Jiang M,Qureshi SA

    更新日期：1998-12-01 00:00:00

  • Variable keratin polypeptide profile in human stratum corneum.

    abstract：:Stratum corneum samples obtained from 46 members in three generations of seven families were analyzed for keratin pattern by gel electrophoresis. All these samples of apparently normal upper arm skin expressed the 55 kDa, 56.5 kDa, and 65 kDa keratin proteins; while only 28%, 20% and 48% of the samples expressed the 5...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/0923-1811(90)90118-w

    authors： Yaar M,Matoltsy GA,Gilchrest BA

    更新日期：1990-07-01 00:00:00

  • Treatment with TNF-α inhibitor rectifies M1 macrophage polarization from blood CD14+ monocytes in patients with psoriasis independent of STAT1 and IRF-1 activation.

    abstract：BACKGROUND:Psoriasis is a systemic inflammatory disease with dramatic responses to TNF-α inhibitors. TNF-α is mainly produced by macrophages. However, how macrophage polarization contributes to psoriasis remains unknown. OBJECTIVE:We aimed to investigate the molecular mechanisms of macrophage polarization in psoriasis...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2018.05.009

    authors： Lin SH,Chuang HY,Ho JC,Lee CH,Hsiao CC

    更新日期：2018-09-01 00:00:00

  • Novel ex vivo disease model for extramammary Paget's disease using the cancer tissue-originated spheroid method.

    abstract：BACKGROUND:Extramammary Paget's disease (EMPD) is a rare skin cancer that frequently occurs in the anogenital region in the elderly. Prognosis in patients with metastatic EMPD is poor as EMPD treatment has advanced little in recent years, primarily because no EMPD cell line has been established. OBJECTIVE:We aimed to ...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2020.07.006

    authors： Arita T,Kondo J,Kaneko Y,Tsutsumi M,Kanemaru M,Matsui M,Arakawa Y,Katoh N,Inoue M,Asai J

    更新日期：2020-09-01 00:00:00

  • Oral supplementation with fish oil reduces dryness and pruritus in the acetone-induced dry skin rat model.

    abstract：BACKGROUND:Pruritus and discomfort are often present in patients with xerosis and atopic dermatitis. Several studies suggest an important role of diet in skin pathophysiology. OBJECTIVE:This study evaluated the effect of dietary fatty acids in the skin physiology via an itch-related animal model with and without suppl...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2015.06.015

    authors： Barcelos RC,de Mello-Sampayo C,Antoniazzi CT,Segat HJ,Silva H,Veit JC,Piccolo J,Emanuelli T,Bürger ME,Silva-Lima B,Rodrigues LM

    更新日期：2015-09-01 00:00:00

  • Tight junction dysfunction in the stratum granulosum leads to aberrant stratum corneum barrier function in claudin-1-deficient mice.

    abstract：BACKGROUND:Tight junctions (TJs) contribute to the epithelial barrier function by preventing leakage of solutes through the intercellular space. In the skin, TJs occur in the stratum granulosum (SG), where claudin-1 and claudin-4 are expressed as adhesion molecules of TJs. Claudin-1-deficient (Cldn1(-/-)) mice die with...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/j.jdermsci.2013.01.002

    authors： Sugawara T,Iwamoto N,Akashi M,Kojima T,Hisatsune J,Sugai M,Furuse M

    更新日期：2013-04-01 00:00:00

  • Activation of fibroblast proliferation by Werner's syndrome fibroblast-conditioned medium.

    abstract：:The effects of Werner's syndrome (WS) fibroblast-conditioned medium on cell proliferation and collagen production of normal fibroblasts were studied using four WS fibroblast strains. The conditioned medium from the WS fibroblasts brought about activation of normal fibroblast proliferation, whereas, that from late pass...

    journal_title：Journal of dermatological science

    pub_type： 杂志文章

    doi：10.1016/0923-1811(94)90097-3

    authors： Hatamochi A,Arakawa M,Takeda K,Ueki H

    更新日期：1994-06-01 00:00:00