Abstract:
:Type 2 iodothyronine deiodinase, an enzyme involved in the conversion of thyroxin to the biologically active 3,5, 3'-triiodothyronine, is highly concentrated in a group of specialized ependymal cells, tanycytes, lining the wall and floor of the third ventricle. As this distribution is highly reminiscent of the distribution of cells containing the phosphatase inhibitor, DARPP-32, we raised the possibility that these two proteins may coexist in tanycytes and that DARPP-32 may modulate type 2 deiodinase activity by regulating the phosphorylation state of the cAMP regulatory factor, CREB. To address this question, double-labeling histochemical studies were performed for type 2 deiodinase mRNA and DARPP-32 immunoreactivity (IR), or DARPP-32- and CREB-IR in the same tissue sections. Type 2 deiodinase mRNA was found in the cell bodies of all DARPP-32-immunolabeled tanycytes. Both type 2 deiodinase mRNA and DARPP-32-IR also extended into tanycyte processes that ramified in the arcuate nucleus and median eminence, in close association with blood vessels and portal capillaries. In contrast, type 2 deiodinase mRNA was not present in the same cells that contained DARPP-32-IR in the pituitary gland. All tanycytes containing DARPP-32-IR also contained CREB-IR in their nucleus. Since type 2 deiodinase activity can be induced by substances that increase cAMP, we hypothesize that DARPP-32 may regulate the activity of type 2 deiodinase by prolonging the activation of CREB. Selectivity for the colocalization of these factors to tanycytes but not the pituitary gland, may explain the heterogeneous response of type 2 deiodinase activity in these two loci in response to specific stimuli such as fasting.
journal_name
Brain Resjournal_title
Brain researchauthors
Fekete C,Mihály E,Herscovici S,Salas J,Tu H,Larsen PR,Lechan RMdoi
10.1016/s0006-8993(00)02105-3subject
Has Abstractpub_date
2000-04-17 00:00:00pages
154-61issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(00)02105-3journal_volume
862pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Migraine and anxiety disorders are frequently co-morbid with balance disorders. This study examined the relative distribution of subtypes of serotonin (5-HT) receptor in the inner ear of monkeys and rats. Most vestibular ganglion cells were immunoreactive for 5-HT(1B) and 5-HT(1D) receptors in macaques and rats. In th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.05.057
更新日期:2010-07-30 00:00:00
abstract::The blink reflex consists of an early, pontine R1-component and a late, medullary R2-component. R1 and R2 can be evoked by innocuous stimuli, but only the R2 also by painful heat, suggesting that the R2 is mediated by wide dynamic range neurons (WDR) of the spinal trigeminal nucleus. Remote noxious stimuli suppress th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00646-5
更新日期:1998-08-24 00:00:00
abstract::A major effect of volatile anesthetics is to reduce excitatory synaptic transmission. In the present study the stimulated release of glutamate under the influence of increasing concentrations of isoflurane was studied in vitro by utilizing hippocampal slices from Wistar rats. Ca(2+)-dependent release was calculated by...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91282-3
更新日期:1994-11-14 00:00:00
abstract::Recent experimental data have shown that hyperbaric oxygen therapy (HBOT) was associated increased Bcl-2 expression at the injury site that correlated with reduced apoptosis. We hypothesized that HBOT mediated enhancement of Bcl-2 expression and increased intracellular oxygen bio-availability may both contribute to pr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.04.078
更新日期:2008-07-24 00:00:00
abstract::Sustained seizure activity can be induced in rabbit olfactory bulb by application of brief low-level current via chronically implanted micro-electrodes. Sensitivity to such stimulation is markedly augmented by prior surgical isolation of the ipsilateral or contralateral bulb. The isolated rabbit olfactory bulb appar...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(75)90056-6
更新日期:1975-08-29 00:00:00
abstract::We examined the effect of nitric oxide (NO) on the expression of phosphorylated cAMP response element binding (pCREB) protein and on the polysialylated form of the neural cell adhesion molecule (PSA-NCAM), in the hippocampus of the adult rat, by administering 7-nitroindazole (7-NI) for 5 days, to block neuronal nitric...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.01.055
更新日期:2004-05-15 00:00:00
abstract::Antagonism of the alpha receptor sub-type at gamma-aminobutyric acid (GABA) recognition sites in developing pigs was evaluated using the GABAA receptor antagonist bicuculline. The effect of bicuculline infusions was to produce an increase of phrenic and hypoglossal discharge amplitudes. This bicuculline-induced effect...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90222-4
更新日期:1994-07-04 00:00:00
abstract::An in vitro radioenzymatic assay and purification procedure by thin-layer chromatography were used to study the metabolism of dihydrotestosterone (DHT) into 3 alpha- and 3 beta-androstanediols by the brain and cloacal gland of Japanese quail. Kinetic studies showed that these 2 metabolites are produced in a linear fas...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91280-7
更新日期:1987-09-22 00:00:00
abstract::C3H/HeJ mice are refractory to lipolysaccharide (LPS) in the periphery, primarily because their macrophages do not respond to LPS and produce pro-inflammatory cytokines such as interleukin-1 (IL-1). To determine if they are also refractory to LPS in the brain, behavior of C3H/HeJ mice was compared to LPS-sensitive C3H...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)01454-0
更新日期:1997-03-28 00:00:00
abstract::Polyamines play critical roles during the development of brain neurons. In the present study we examined the effects of polyamines on neuronal apoptotic death. Rat cerebellar granule neurons were cultured in the presence of a depolarizing concentration of KCl (25 mM) in the medium. Apoptotic neuronal death was induced...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00011-5
更新日期:1997-04-11 00:00:00
abstract::Most functional neuroimaging techniques rely on activation-flow coupling (AFC) to detect changes in regional brain function, but AFC responses may also be altered during pathophysiological conditions such as ischemia. To define the relationship between progressive ischemia and the AFC response, graded levels of cerebr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.04.024
更新日期:2005-06-14 00:00:00
abstract::We identified the burst and single-spiking cells, and the repetitive bursting cells in layers III and V of the cat sensorimotor cortex with intracellular recording and staining techniques. Both types of the bursting cells were found in 22.7% of the recorded layer V neurons and in 23.1% of the recorded neurons in layer...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00505-7
更新日期:1996-07-15 00:00:00
abstract::Excessive stimulation of neurons by glutamic acid initiates a destructive cascade of ion fluxes, cellular swelling, and death. Homeostatic mechanisms which rectify these disturbances depend largely upon transmembrane ion gradients maintained by Na+,K(+)-ATPase (NaP). We proposed that the neurotoxicity of glutamate is ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90982-f
更新日期:1992-09-18 00:00:00
abstract::A major mesolimbic projection towards the hippocampal formation (HF) has been extensively described, but no clear evidence of its dopaminergic content has been demonstrated. In order to evaluate the percentage of dopaminergic (DA) cells of ventral tegmental area (VTA-A10) and adjacent substantia nigra (SN-A9) projecti...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90512-6
更新日期:1994-12-30 00:00:00
abstract::To investigate the effects of ionic changes possibly associated with hibernation, hippocampal slices prepared from golden hamsters were studied in artificial cerebrospinal fluid (ACSF) of variable composition (K+ 3-5 mM, Ca2+ 2-4 mM, Mg2+ 2-4 mM, pH 7.0-7.7) at temperatures of 15-20 degrees C, just above the temperatu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00524-t
更新日期:1995-08-14 00:00:00
abstract::These experiments examined the effects of intra-amygdala injections of NMDA receptor antagonists on the acquisition and retention of inhibitory avoidance. In Expt. I, rats received bilateral intra-amygdala injections of the NMDA antagonists D,L-AP5 (1-10 micrograms), D-AP5 (0.03-1 micrograms), CPP (0.125 or 0.375 micr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91188-k
更新日期:1992-07-10 00:00:00
abstract::In our previous study, we have found that leucine-rich repeats and immunoglobulin-like domains 1(LRIG1) can improve the chemosensitivity in U251 cells whereas the role of LRIG1 in multidrug resistance (MDR) remains unknown. Here, we reported that LRIG1 can reverse MDR by inhibiting epidermal growth factor (EGF) recept...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.03.023
更新日期:2015-06-22 00:00:00
abstract::Drug addiction is characterized by persistent relapse vulnerability during abstinence. In abstinent drug users, relapse is often precipitated by re-exposure to environmental contexts that were previously associated with drug use. This clinical scenario is modeled in preclinical studies using the context-induced reinst...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2014.09.004
更新日期:2015-12-02 00:00:00
abstract::Immunohistochemical studies with an antiserum raised against tyrosine hydroxylase have allowed us to demonstrate a dense dopaminergic innervation of the globus pallidus in the squirrel monkey. This innervation derived mostly from two fascicles that detached themselves from the major ascending dopaminergic bundle arisi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90896-1
更新日期:1987-11-24 00:00:00
abstract::The neuroprotective effect of tachykinins against excitotoxic death of cholinergic neurons was studied in rat striatal cell cultures. Quinolinic acid (QUIN) and kainic acid (KA) produced a dose dependent decrease in choline acetyltransferase activity, but KA was more potent. Our results show that substance P (SP) tota...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)00879-7
更新日期:1996-11-18 00:00:00
abstract::Immunohistochemical staining with mouse monoclonal antibody R24 recognizing the ganglioside GD3 was used to study the distribution of GD3 in the human brain. Positive staining was primarily found in the surrounding neuropil of many neuronal groups in the brainstem and spinal cord, cerebellum, retina and dentate gyrus ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90642-5
更新日期:1984-12-17 00:00:00
abstract::This study aimed to identify sites and mechanisms of long-term plasticity following lower limb muscle training. Two groups performing either a postural stability maintenance training (SMT) or a ballistic ankle strength training (BST) were compared to a non-training group. The hypothesis was that practicing of a self-i...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.08.048
更新日期:2007-11-07 00:00:00
abstract::Using a co-culture model, we showed that diffusible factors from arcuate nucleus (AN) specifically increased the number and the size of hypothalamic neurons producing melanin-concentrating hormone (MCH). In this model neurite outgrowth and contacts between MCH neurons and dopaminergic neurons were also prominently inc...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90948-m
更新日期:1993-11-19 00:00:00
abstract::A short-acting non-barbiturate intravenous general anaesthetic, etomidate, when administered i.v. lowered serum prolactin levels in the rat. The effect was evident at rest, after surgical stress or after injection of 5-hydroxytryptophan. Haloperidol-induced hyperprolactinaemia was not modified. Administered via the in...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90306-2
更新日期:1983-07-25 00:00:00
abstract::Diverse neuropsychiatric diseases were recently linked to specific anti-neuronal autoantibodies targeting synaptic proteins. Symptoms can range from epileptic seizures to cognitive impairment to movement disorders, commonly responding to treatment with immunotherapy. Several of these autoantibodies target inhibitory s...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2018.09.009
更新日期:2018-12-15 00:00:00
abstract::Glucagon like peptide-1 (7-36) amide (GLP-1), a potent regulator of glucose homeostasis, is also produced in the central nervous system and has been implicated in the control of hypothalamic-pituitary function and food intake. GLP-1 immunoreactive (IR) fibers and terminals are widely distributed in the septum, hypotha...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(03)03117-2
更新日期:2003-09-26 00:00:00
abstract::Opioid modulation of ingestion includes general opioid antagonism of deprivation-induced water intake and intake of sucrose and saccharin solutions. Previous studies using selective subtype antagonists indicated that opioid effects upon deprivation-induced water intake occurred through the mu2 receptor and that opioid...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91274-v
更新日期:1993-08-06 00:00:00
abstract::We have developed a computer-assisted method for analyzing the 2-deoxyglucose (2-DG) autoradiograms of mice olfactory bulbs. The purpose of the study was to numerize the maps of glomerular activation in order to achieve a statistical comparison of the glomerular patterns evoked by different stimuli. The spatial distri...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90173-9
更新日期:1987-08-04 00:00:00
abstract::The discriminative stimulus properties of ethanol are functionally regulated by ionotropic GABA(A) and NMDA receptors in specific limbic brain regions including the nucleus accumbens, amygdala, and hippocampus, as determined by microinjection studies. The purpose of the present work was to further investigate potentia...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.07.061
更新日期:2008-09-26 00:00:00
abstract::The intercollicular pathway of the rat was studied using autoradiographic (ARG) and horseradish peroxidase (HRP) tracing techniques. The HRP experiments demonstrated that the cells of origin of the intertectal pathway were located primarily in the rostral stratum griseum intermediale ( SGI ), stratum album intermedium...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90848-5
更新日期:1984-05-23 00:00:00