DNA analysis of malignant salivary gland carcinomas: comparison of different tissue preparations and measuring techniques.

Abstract:

:This study correlates static and flow-cytometric analysis on paraffin-embedded and fresh material of salivary gland carcinomas. Nuclear suspensions for DNA analysis were prepared from paraffin-embedded and fresh material. Comparison of the results of static and flow cytometry on paraffin-embedded material revealed no significant correlation between DNA ploidy and S-phase value. The coefficients of variation were significantly lower for static than for flow cytometry (p < 0.05). Flow-cytometric analysis on fresh and paraffin-embedded material correlated well concerning DNA ploidy, but not for the S-phase. The coefficients of variation were significantly lower for the fresh than for the paraffin-embedded material (p < 0.001). Cytometric analysis on paraffin-embedded material of malignant salivary gland carcinomas should be critically evaluated. There is a need for flow-cytometric investigations on fresh material to clarify further the prognostic value of this method.

journal_name

Acta Otolaryngol

journal_title

Acta oto-laryngologica

authors

Nordgård S,Franzén G,Boysen M,Tytor M

doi

10.1080/00016489950181080

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

510-5

issue

4

eissn

0001-6489

issn

1651-2251

journal_volume

119

pub_type

杂志文章
  • Quantitative evaluation of pinocytosis of capillaries of the stria vascularis under normal and experimental conditions.

    abstract::In order to examine evidence of high activity of pinocytosis in capillaries of the stria vascularis, quantitative morphometry of pinocytotic vesicles was carried out ultrastructurally by the horseradish peroxidase (HRP) tracer method. In guinea pigs, there was a significant difference between the stria vascularis and ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:

    authors: Sakagami M,Harada T,Sano M,Sakai S,Matsunaga T

    更新日期:1987-03-01 00:00:00

  • Patterns of framework invasion in patients with laryngeal cancer: correlation of in vitro magnetic resonance imaging and pathological findings.

    abstract::Total laryngectomy for laryngeal squamous cell carcinoma means a drastic change in the way of living for a patient. New surgical techniques such as laser surgery attempt to save the voice. To be oncologically correct, an accurate assessment of the tumor extent is necessary. Imaging is especially important in determini...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016489850182648

    authors: Declercq A,Van den Hauwe L,Van Marck E,Van de Heyning PH,Spanoghe M,De Schepper AM

    更新日期:1998-11-01 00:00:00

  • Endoscopic endonasal resection and radiotherapy as treatment for skull base chordomas.

    abstract:BACKGROUND:The role of endonasal endoscopic surgery and radiotherapy in the treatment of skull base chordomas remains unclear. OBJECTIVE:We investigated the effect of endonasal endoscopic surgery and radiotherapy as treatment for skull base chordomas. METHOD:We investigated 46 patients (2006-2018) treated at the Affi...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016489.2020.1748225

    authors: Li H,Zhang H,Hu L,Wang H,Wang D

    更新日期:2020-09-01 00:00:00

  • [Oscillometry on various stape protheses. Experimental examination of human temporal bone preparations].

    abstract::In human temporal bone specimens the vibrations of the labyrinthine fluid were measured by a piezoelectric system after application of various techniques of otosclerosis surgery. Compared with the amplitude in the case of a normal stapes, the surgical techniques according to Zangemeister, Shea and Schuknect resulted i...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016487509124656

    authors: Cancura W

    更新日期:1975-01-01 00:00:00

  • Elevated levels of myelin basic protein in CSF in relation to auditory brainstem responses in Bell's palsy.

    abstract::Myelin basic protein (MBP) in the cerebrospinal fluid (CSF) was quantified from 28 patients with acute facial palsy. The mean value of MBP in CSF in the study group was 3.4 +/- 0.22 micrograms/l (mean +/- SEM), which was significantly higher than the found in 37 healthy subjects (2.4 +/- 0.13 micrograms/l). Using a 95...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:

    authors: Edström S,Hanner P,Andersen O,Rosenhall U,Vahlne A,Karlsson B

    更新日期:1987-03-01 00:00:00

  • Heterologous epidermal growth factor receptor (EGF-R) expression in larynx cancer cell lines: evidence for the existence of structurally modified receptors.

    abstract::Overexpression of epidermal growth factor receptor (EGF-R) is a common characteristic of epidermoid tumors and its expression in head and neck squamous cell carcinoma biopsies and cell lines has been reported previously by several authors. With the aim to provide more structural and functional details about the protei...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489309135902

    authors: Bujía J,Wustrow TP

    更新日期:1993-11-01 00:00:00

  • Cuff pressure and microvascular occlusion in the tracheal mucosa. An intravital microscopic study in the rabbit.

    abstract::A method is described for the intravital microscopic observations of rabbit tracheal mucosa microcirculation during compression with a thin, transparent high volume cuff. The cuff pressure (CP) required to cause complete ischemia in the mucosa over the cartilages was measured and a correlation was found to the mean ar...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016487909137192

    authors: Stenqvist O,Bagge U

    更新日期:1979-01-01 00:00:00

  • The roles of local inhibition mediated by gamma-aminobutyric acid (GABA)-A receptor in duration tuning in the inferior colliculus of guinea pigs.

    abstract:CONCLUSION:GABA-mediated inhibition is responsible for the duration tuning in the inferior colliculus (IC) of guinea pigs, a non-echo-locating mammal. Duration tuning in this species is better demonstrated in an appropriate short time window. OBJECTIVES:To investigate the role of GABA-mediated inhibition in duration t...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016480701840122

    authors: Yin S,Chen Z,Feng Y,Wang J

    更新日期:2008-10-01 00:00:00

  • Clinical trial on tinnitus patients with normal to mild hearing loss: broad band noise and mixed pure tones sound therapy.

    abstract:BACKGROUND:Tinnitus treatments present a quandary for clinicians, but no thoroughly satisfactory medical treatments are offered to tinnitus patients. OBJECTIVE:We compared sound therapy effects of the broad band noise (BBN) and the mixed pure tones on tinnitus patients with normal to mild hearing loss, and the possibl...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章,随机对照试验

    doi:10.1080/00016489.2019.1575522

    authors: Li Y,Feng G,Wu H,Gao Z

    更新日期:2019-03-01 00:00:00

  • Evaluation of phenotype of mononuclear host cells isolated from primary tumour and peripheral blood of patients with laryngeal carcinoma.

    abstract::Mononuclear host cells isolated from primary laryngeal carcinoma were assessed by means of indirect immunofluorescence with a panel of monoclonal antibodies against various lymphocyte subsets and macrophages. Tumours of various staging groups were examined in parallel with cells isolated from patient and donor periphe...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016488809119459

    authors: Zeromski J,Pietrzak J,Szmeja Z,Jezewska E,Górny MK,Kruk-Zagajewska A

    更新日期:1988-01-01 00:00:00

  • Experimental recurrent pneumococcal otitis media. Protection and serum antibodies.

    abstract::To elucidate immune mechanisms in otitis media, middle ear infection was induced in 12 rats by intratympanic inoculation of Streptococcus pneumoniae type 3, either the ipsilateral or the contralateral middle ear being re-challenged 4 weeks later. Otomicroscopy inspection confirmed the presence of acute otitis media (A...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489109100760

    authors: Svinhufvud M,Prellner K,Hermansson A,Schalén C

    更新日期:1991-01-01 00:00:00

  • A comparison between systemic and intratympanic steroid therapies as initial therapy for idiopathic sudden sensorineural hearing loss: a meta-analysis.

    abstract:CONCLUSIONS:Intratympanic steroid (ITS) treatment groups exhibited better outcomes in PTA improvement and recovery rate than systemic steroid therapy (SST) groups. Whether initial hearing loss severity would influence the PTA improvement and recovery rate still requires further research. OBJECTIVE:This article was aim...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章,meta分析

    doi:10.1080/00016489.2016.1260157

    authors: Qiang Q,Wu X,Yang T,Yang C,Sun H

    更新日期:2017-06-01 00:00:00

  • A simple objective evaluation and grading for facial paralysis outcomes.

    abstract:CONCLUSION:A simple measuring box to determine lip-shift angle and eye-width narrowing offered simultaneous and objective data on outcome dysfunction. The method is as easy as visual approaches. The method is objective and as easy as visual access. It is practical for routine clinical use in any clinic. OBJECTIVES:The...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489.2011.626793

    authors: Saito H

    更新日期:2012-01-01 00:00:00

  • The release of histamine from the pars flaccida mast cells one cause of otitis media with effusion?

    abstract::In this experimental study in the rat, compound 48/80--a potent degranulating drug of mast cells--was instilled in the external auditory meatus towards the tympanic membrane. Moreover, effusion material was produced in the attic space by blocking the tympanic isthmus with a piece of Gelfoam applied through the tympani...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016488209128942

    authors: Alm PE,Bloom GD,Hellström S,Salén B,Stenfors LE

    更新日期:1982-11-01 00:00:00

  • Hydrops of the organ of Corti.

    abstract:OBJECTIVES:The authors would like to confirm a fluid pathway from the scala tympani to the organ of Corti, and to observe its morphological changes. METHODS:A staining solution for succinic dehydrogenase was perfused with phenazine methosulfate in the scala tympani of living guinea pigs (n = 5) under deep anesthesia. ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016489.2016.1185541

    authors: Nomura Y,Kimura Y,Kobayashi H

    更新日期:2016-11-01 00:00:00

  • The influence of cochlear temperature on the electrical travelling wave pattern in the guinea pig cochlea.

    abstract::Cochlear microphonics (CM) were recorded in the guinea pig using differential recording from the first and third cochlear turns as well as by using a 10-electrode array inserted in the scala tympani of the basal turn. The excitation profile as represented by the CM was quantified by amplitude and phase data, and was m...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016487809121465

    authors: de Brey HB,Eggermont JJ

    更新日期:1978-05-01 00:00:00

  • Incidence of thyroid gland invasion in advanced laryngeal cancers and its impact on disease-specific survival; a retrospective review at a tertiary care center.

    abstract::Background: Incidence of thyroid gland invasion in advanced laryngeal cancers is low. Ipsilateral or total thyroidectomy along with total laryngectomy has been controversial and there has been no consensus over the management of thyroid gland in advanced laryngeal cancers.Objective: To determine the frequency of thyro...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016489.2020.1778786

    authors: Muhammad T,Dhanani R,Mohtasham S,Hussain M,Faisal M,Malik KI,Jamshed A,Hussain R

    更新日期:2020-10-01 00:00:00

  • Recovery period of sinonasal quality of life and its associated factors after endoscopic endonasal approach for anterior skull base tumors.

    abstract:BACKGROUND:Although endoscopic endonasal approach (EEA) for skull base tumors showed a good prognosis in sinonasal quality of life (QOL), what factors have influence on QOL is still in question. Aim/Objectives: To investigate the recovery of sinonasal QOL after EEA for anterior skull base tumors and find its prognostic...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016489.2019.1574982

    authors: Ahn JC,Cho SW,Kim DK,Han DH,Kim DY,Rhee CS,Lee CH,Kim YH,Paek SH,Won TB

    更新日期:2019-05-01 00:00:00

  • Characterization of AMC-HN-9, a cell line established from an undifferentiated carcinoma of the parotid gland: expression of alpha6beta4 with the absence of BP180 and 230.

    abstract::We recently reported the development of a cell line, AMC-HN-9, established from an undifferentiated carcinoma (UDC) of the parotid gland. AMC-HN-9 consists mostly of spindle-shaped cells, has poor in vitro adhesiveness and an in vitro appearance that is different from that of other epithelial cell lines. To test the h...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/000164800750000513

    authors: Kim SH,Carey TE,Liebert M,Yoo SJ,Kwon HJ,Kim SY

    更新日期:2000-08-01 00:00:00

  • Repeated courses of intratympanic dexamethasone injection are effective for intractable Meniere's disease.

    abstract:CONCLUSION:Intra-tympanic dexamethasone injection (ITD) could serve as a first-line intra-tympanic (IT) treatment for Meniere's disease (MD), regardless of hearing level. Even if the response to initial ITD course was unsatisfactory, a repeated course may be beneficial with some patients. OBJECTIVES:This study examine...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016489.2016.1224920

    authors: Leng Y,Liu B,Zhou R,Liu J,Liu D,Zhang SL,Kong WJ

    更新日期:2017-02-01 00:00:00

  • Effects of short inter-stimulus intervals on olfactory and trigeminal event-related potentials.

    abstract:CONCLUSION:Chemosensory event-related potentials (CSERPs) were identified in all measurements using the 20 s and the 10 s inter-stimulus interval (ISI) protocol, reducing the recording time to 75% or 57% compared with the standard protocol. A possible explanation for the rising CSERP amplitudes by shortening the ISI in...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016480802644605

    authors: Kassab A,Schaub F,Vent J,Hüttenbrink KB,Damm M

    更新日期:2009-11-01 00:00:00

  • Functional outcomes of Vibrant Soundbridge applied on the middle ear windows in comparison with conventional hearing aids.

    abstract:CONCLUSION:Vibrant Soundbridge (VSB) application to the middle ear windows yields better functional outcomes than conventional hearing aids. However, speech discrimination scores obtained with VSB and conventional hearing aids are similar. OBJECTIVE:To assess audiological outcomes of round and oval window applications...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489.2012.702353

    authors: Gunduz B,Atas A,Bayazıt YA,Goksu N,Gokdogan C,Tutar H

    更新日期:2012-12-01 00:00:00

  • Electrocochleography and cochlear pathology.

    abstract::In experimentally damaged inner ears the structural alterations were correlated to electrocochleographic responses of the ear. Sectioning of the cochlear nerve with degeneration of the type I neurons but intact sensory cells results in normal cochlear microphonics but very weak and atypical nerve responses. By contras...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016487709128822

    authors: Spoendlin H,Baumgartner H

    更新日期:1977-01-01 00:00:00

  • Polyp formation by experimental tubal occlusion in the rat.

    abstract::The purpose of this study was to show the early stages of polyp formation, as part of our studies on the pathogenesis of nasal polyps. Two and 4 weeks after experimental tubal occlusion in 18 Wistar rats, 13 showed signs of bacterial infection, respective acute otitis media. In the majority of these rats polypouslike ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489109138432

    authors: Larsen PL,Tos M

    更新日期:1991-01-01 00:00:00

  • Histopathological observations in chronic maxillary sinusitis.

    abstract::Biopsy samples from the maxillary sinus mucosa of patients with purulent, non-purulent and recurrent sinusitis were studied histologically and compared with those from a maxillary sinus of patients with no previous history of sinus disease on the side of sampling. Basement membrane thickening, atypical gland formation...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489009107468

    authors: Stierna P,Carlsöö B

    更新日期:1990-11-01 00:00:00

  • Lexical tone identification and consonant recognition in acoustic simulations of cochlear implants.

    abstract:CONCLUSION:Cochlear implant (CI) recipients' performance of lexical tone identification and consonant recognition can be enhanced by providing greater spectral details. OBJECTIVE:To evaluate the effects of increasing the number of total spectral channels on the lexical tone identification and consonant recognition by ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016480802032793

    authors: Lin YS,Lu HP,Hung SC,Chang CP

    更新日期:2009-06-01 00:00:00

  • Gravity load related asymmetries in the sagittal vestibulo-collic reflex.

    abstract::EMG recordings of the neck muscles (biventer cervicis, complexus, splenius, longus capitis) of decerebrate cats were obtained during pitch and roll stimulations (sinusoidal stimulation: 30 degrees p-p amplitude, 0.2 Hz frequency). Most of the EMG responses to pitch showed activation peaks leading the position stimuli ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489309135800

    authors: Pettorossi VE,Santarelli R,Draicchio F,Errico P,Ferraresi A

    更新日期:1993-05-01 00:00:00

  • Intracochlear electrical tinnitus reduction.

    abstract::Several reports have indicated that some cochlear implant patients experience a reduction in their tinnitus while listening to noise or speech. In the present study, two patients reporting bilateral tinnitus were selected from a group of adults with the Nucleus cochlear implant. They rated their tinnitus loudness and ...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016489309135811

    authors: Dauman R,Tyler RS,Aran JM

    更新日期:1993-05-01 00:00:00

  • Convergence of central and peripheral signals on vestibular cells.

    abstract::Nerve and cortical input convergence patterns, representing the fore- and hindlimb, were studied in single cells located in the lateral vestibular nucleus of the cat. Deitersian cells responded with excitation to cortical stimulation with a latency ranging from 4 to 14 msec, while responses ranged from 9 to 12 msec to...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.3109/00016488309130942

    authors: Azzena GB,Mameli O,Tolu E

    更新日期:1983-03-01 00:00:00

  • Traumatic retropharyngeal haematoma in an anticoagulated patient: Case report and proposal for a clinical protocol.

    abstract::Retropharyngeal haematomas (RPHs) are rare but potentially life-threatening conditions that require a prompt diagnosis. However, the clinical scenario is not always straightforward as their presentation may be insidious, with no specific signs or symptoms. Treatment of RPH is conservative in the majority of cases, wit...

    journal_title:Acta oto-laryngologica

    pub_type: 杂志文章

    doi:10.1080/00016480410017233

    authors: Chiti-Batelli S,Vaz F,Coman S

    更新日期:2005-04-01 00:00:00