Abstract：
:P-glycoprotein has been identified in mast cells stabilized in culture as well as in rat peritoneal mast cells, and is primarily concentrated on the granular membrane. This study aimed to define the role of this protein in the transport and accumulation of doxorubicin in mast cell granules and in its histamine releasing effect. The reverting agent verapamil, that is a substrate for P-glycoprotein, inhibited doxorubicin uptake in intact mast cells in a dose and time dependent manner, but had no effect on the exocytotic action of the antineoplastic drug. Doxorubicin was also concentrated in granules with intact membranes and the uptake was dependent on temperature and showed a trend for saturation. Verapamil and vinblastine, another substrate for P-glycoprotein, significantly reduced doxorubicin concentrations in intact granules. Similar results were obtained with the metabolic inhibitors sodium metavanadate, N-ethylmaleimide, and sodium azide, whereas ouabain, an inhibitor of sodium-potassium ATPase, was without effect. Doxorubicin was taken also up in granule remnants, consisting of a proteoglycan matrix without membrane, that are extruded from mast cells upon stimulation. However, the uptake was not dependent on temperature and was not modified by P-glycoprotein substrates or metabolic inhibitors. Rat peritoneal mast cells were examined for the expression of P-glycoprotein at the protein level with C219 monoclonal antibody, using Western blot, confirming that P-glycoprotein was expressed in mast cells. These data suggest the presence of a P-glycoprotein active in the transport of doxorubicin, in mast cell granules.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Candussio L,Decorti G,Crivellato E,Rosati AM,Traversa U,Klugmann FBsubject
Has Abstractpub_date
1999-03-01 00:00:00pages
1101-8issue
2Aeissn
0250-7005issn
1791-7530journal_volume
19pub_type
杂志文章abstract：:The in vivo investigation of onco/suppressor gene effects may provide new information on chemical-environmental carcinogenesis. We previously described the elevation of onco/suppressor gene expression due to CHOP and COPP chemotherapeutical protocols in a CBA/Ca mouse model. Below we describe the results of the onco/s...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:We describe a very rare case of recurrent hepatocellular carcinoma (HCC) after living donor liver transplantation (LDLT). A 47-year-old female underwent LDLT for HCC within Milan criteria, receiving a left liver lobe from her 38-year-old husband with an incompatible blood type. Thirty months after LDLT, however, enhan...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
abstract：:The tumor progression concept was first defined by Foulds at the end of the nineteen fifties. His work about the evolution of mammary tumors led to the conclusion that a tumor is initiated as soon as a cell interacts with an oncogenic agent and that the tumor progresses by acquiring in a permanent and irreversible way...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：BACKGROUND/AIM:The present study aimed to identify hypoxia-regulated microRNAs (HRMs) in vitro and investigate the clinical role of candidate HRMs in patients with gastroesophageal cancer (GEC). MATERIALS AND METHODS:microRNA expression changes induced by hypoxia in human GEC cell lines were measured with microarrays ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-02-01 00:00:00
abstract：:The antitumor effect of endogenous tumor necrosis factor (en-TNF) with cyclophosphamide (CY) was analyzed using the murine Meth A tumor model. En-TNF was induced by the administration of interferon-gamma (4 micrograms/kg: 1 x 10(4) units/ mouse) as a primer and Streptococcus preparation OK-432 (100 KE/kg) as a trigger...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:We previously showed the usefulness of a fused protein of human pancreatic ribonuclease1 (hRNase1) with human epidermal growth factor (hEGF) for molecular targeting of EGF receptor (EGFR)-overexpressing cells. In this study, the mechanisms underlying the inhibition of cell growth by RNase-EGF fused proteins ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-03-01 00:00:00
abstract：:Tumor-specific antigens are important in the study of tumor biology, tumor diagnosis and prognosis and possibly in tumor therapy. We demonstrate in this report that surgical specimens of colon, breast and ovarian tumors in native-state in vitro gel-supported three-dimensional in vitro histoculture maintain in vivo-lik...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：BACKGROUND/AIM:Gemcitabine is a drug commonly used to treat pancreatic cancer but chemoresistance to it is a common clinical issue. KML001 (sodium meta-arsenite) has demonstrated certain antitumor activity. The objective of the study was to evaluate the influence of KML001 on the anticancer activity of gemcitabine agai...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:A gastrostomy feeding tube is one method for long-term feeding support in patients undergoing radio(chemo)therapy for head and neck cancer (HNC). The aim of this study was to analyze the safety of prophylactic gastrostomy tube placement and usage in HNSCC patients. PATIENTS AND METHODS:HNC patients undergoi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14059
更新日期：2020-02-01 00:00:00
abstract：:The influence of several oncogenes on drug resistance has recently been show by transfection studies. Therefore, we investigated the influence of transfection on adriamycin (ADR) in cell line transfected with several oncogenes accompanied with pSV2neo vector. There were differences in both the intracellular accumulati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：AIM:Metabolic syndrome has been identified as a risk factor for colorectal cancer and adenoma. The aim of our study was to assess the risk of colorectal cancer and adenoma in an adult Italian population with metabolic syndrome. PATIENTS AND METHODS:Ninety patients with metabolic syndrome were prospectively compared ag...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-04-01 00:00:00
abstract：:We have examined the effects of irradiation on the cytokine secretion from genetically modified human esophageal and gastric carcinomas. Both cell lines were transduced retrovirally to secrete interleukin-2, interleukin-6 and granulocyte-macrophage colony-stimulating factor, respectively. The metabolism of all the tra...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：:Experimental biochemical modulation of 1-hexylcarbamoyl-5-fluorouracil (HCFU) with l-leucovorin (LV) was carried out using human gastric (H-111) and colon (Co-4) carcinoma xenografts serially transplanted into nude mice. Thirty-five or 70 mg/kg HCFU dissolved in 0.2 ml of 1% hydroxymethyl cellulose was administered po...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：UNLABELLED:The aim of this study was to evaluate the efficacy and safety of erlotinib, an epidermal growth factor receptor tyrosine kinase inhibitor, in Japanese patients with relapsed or recurrent advanced non-small cell lung cancer (NSCLC). PATIENTS AND METHODS:This was a multicentre, open-label phase II study of er...
journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：2010-02-01 00:00:00
abstract：BACKGROUND:Certain viruses, notably human papillomavirus (HPV), cytomegalovirus (CMV), herpes simplex virus (HSV) and Epstein-Barr virus (EBV), are known to produce tumors in animals and cell transformation in vitro and they have been implicated in the pathogenesis of human cancers. All these viruses are also known to ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:Oral mucositis is one of the main complications in non-surgical cancer treatments. It represents the major dose-limiting toxicity for some chemotherapeutic agents, for radiotherapy of the head and neck region and for some radiochemotherapy combined treatments. Many reviews and clinical studies have been published in o...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2007-03-01 00:00:00
abstract：BACKGROUND/AIM:To assess prognosis of women aged 75 and older according to breast cancer (BC) diagnosis circumstances. PATIENTS AND METHODS:A retrospective cohort study was conducted in the Amiens, France, regional oncologic referral center between 2005 and 2015. Two groups were formed depending on whether the patient...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11397
更新日期：2017-02-01 00:00:00
abstract：BACKGROUND:Methylenetetrahydrofolate reductase (MTHFR) plays a role in DNA biosynthesis, methylation and repair in actively dividing cells by acting on folate metabolism. A common C677T polymorphism in the gene for MTHFR leads to an enzyme with decreased activity. MTHFR polymorphisms have been studied in various cancer...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:Ewing's sarcoma cell lines may represent a good in vitro model for the understanding of tumor biology in this heterogeneous group of diseases. In the present study, we report the establishment and characterization of a primary Ewing's sarcoma cell line (LDS-Falck 01). MATERIALS AND METHODS:LDS-Falck 01 was ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：AIM:The suitability of Papanicolaou staining and of hematoxylin staining for DNA single-cell cytometry was investigated in comparison to Feulgen staining. MATERIALS AND METHODS:Ten normal cervical smears and ten cervical smears containing cells of a squamous cell carcinoma in situ were analyzed. The integrated optical...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND/AIM:Pelvic exenteration is a radical procedure for certain advanced or recurrent gynaecological cancers, performed with curative or palliative intent. Its validity has evolved as operative mortality and morbidity have improved. This surgery was evaluated to determine the validity of these claims. PATIENTS A...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13759
更新日期：2019-10-01 00:00:00
abstract：:Variants of the Bomirski family of hamster melanomas whose proliferative rates differ inversely with the genetically determined degree of melanogenesis were probed for two proteins critical in melanogenesis: tyrosinase and catalase-B (gp 75). The parental black tumor Ma contained both proteins in abundance. The amelan...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:The effects of dehydroepiandrosterone (DHEA) and 5-en-androstene-3 beta, 17 beta-diol (ADIOL) on the proliferation of MCF-7 cells were studied both in steroid - free and estradiol (E2) supplemented media. Growth was evaluated by counting the cells after six days of culture. The results show that DHEA 500 nM and ADIOL ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:MicroRNAs (miRNA) are a class of small noncoding RNAs that regulate the expression of their target genes. The aim of the present study was to explore the effects of miR-206 on laryngeal suamous cell carcinoma (LSCC) cells. The expression level of miR-206 was quantified by qRT-PCR in primary LSCC tissues and correspond...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND:Amrubicin is an active agent for the treatment of small-cell lung cancer (SCLC). However, there have been no reports of long-term amrubicin use. PATIENTS AND METHODS:Twelve patients with SCLC who were treated with eight or more cycles of amrubicin chemotherapy were retrospectively reviewed. RESULTS:The med...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
abstract：BACKGROUND:Thymidine phosphorylase (dThdPase) is identical to platelet-derived endothelial cell growth factor (PD-ECCF) and has a function of angiogenesis in vitro and in several types of human carcinoma tissues. We have reported that expression of dThdPase was an independent prognostic factor in 116 gastric carcinomas...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：BACKGROUND:Tenascin-C (TNC) is an extracellular matrix glycoprotein, usually derived from myofibroblasts in the cancer microenvironment. Recently, however, the significance of tumor-derived TNC in initiation of cancer metastasis was disclosed. We investigated the clinical significance of cancer-derived TNC in colorecta...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
abstract：BACKGROUND:Neuroendocrine (NE) carcinomas of the lung exhibit expression of various stem cell antigens, and except for carcinoid tumours, carry a poor prognosis. Despite the fact that 10%-30% of all non-small cell lung carcinomas (NSCLC) which are not classified as NE carcinomas also show expression of NE markers, data...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:We have previously shown that transfection of NIH 3T3 cells with the T24 H-ras oncogene converts the cells to a tumorigenic and metastatic phenotype, in proportion to levels of ras expression. We hypothesize that ras-induced increases in malignancy occur via altered expression of various genes. We have identified OPN ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Biotransformation of irinotecan (CPT-11) into its pharmacologic active metabolite SN-38 was investigated in patients treated for advanced colorectal cancer. A dose of 180 mg/m(2) CPT-11 was administered to 6 patients by 60 min hepatic intra-arterial infusion (HAI) via a surgically implanted Port-a-Cath® system. Blood ...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2011-10-01 00:00:00
