当前位置： SCI文献检索 > CLINICAL AND EXPERIMENTAL IMMUNOLOGY期刊下所有文献 > The influence of DNA size on the binding of antibodies to DNA in the sera of normal human subjects and patients with systemic lupus erythematosus (SLE).

The influence of DNA size on the binding of antibodies to DNA in the sera of normal human subjects and patients with systemic lupus erythematosus (SLE).

Abstract：

:To elucidate antibody recognition of DNA in normal and aberrant immunity, the binding of sera of normal human subjects (NHS) and patients with SLE was tested with mammalian and bacterial DNA varying in size. Klebsiella pneumoniae (KP) and calf thymus (CT) single-stranded DNA (ssDNA) were investigated as model antigens using the restriction enzyme HinfI to generate fragments with the size range of 800-5000 base pairs. The influence of size on activity was assessed by ELISA by both titration of serum as well as coating antigen concentration. In both assay formats, SLE sera bound equivalently to intact CT and KP DNA, but had dramatically reduced reactivity to fragments of both antigens. In contrast, NHS bound similarly to intact KP DNA and its fragments but had low reactivity to CT DNA. These results suggest that SLE and NHS anti-DNA react with different antigenic determinants on DNA, as shown by cross-reactivity as well as size dependency in solid-phase assays.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Pisetsky DS,Gonzalez TC

doi

10.1046/j.1365-2249.1999.00875.x

subject

Has Abstract

pub_date

1999-05-01 00:00:00

pages

354-9

issue

2

eissn

0009-9104

issn

1365-2249

journal_volume

116

pub_type

杂志文章

相关文献

CLINICAL AND EXPERIMENTAL IMMUNOLOGY文献大全
  • Hepatic interleuklin 15 (IL-15) expression: implications for local NK/NKT cell homeostasis and development.

    abstract：:Interleukin 15 (IL-15) is critical for the development of human and murine natural killer (NK) cells and hepatic-derived NK T cells (NKT) in mice, and for the homeostatic maintenance of NK/NKT and CD8(+) memory T cells. The lymphocyte repertoire of an adult human liver includes significant populations of NK and NKT-li...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2004.02586.x

    authors： Golden-Mason L,Kelly AM,Doherty DG,Traynor O,McEntee G,Kelly J,Hegarty JE,O'Farrelly C

    更新日期：2004-10-01 00:00:00

  • Isolation of circulating immune complexes by conglutinin and separation of antigen from dissociated complexes by immobilized protein A.

    abstract：:A method for the isolation of complement-fixing immune complexes from human serum and the separation of antigen from antibody is described. In order to isolate the complexes, we used soluble bovine conglutinin in a three-step procedure: (1) serum containing immune complexes is reacted with conglutinin in the presence ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Gupta RC,Tan EM

    更新日期：1981-10-01 00:00:00

  • Bispecific anti-human red blood Rhesus-D antigen x anti Fc gamma RI targeted antibody-dependent cell-mediated cytotoxicity and phagocytosis by mononuclear leucocytes.

    abstract：:The Fc receptor mediated antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis induced by bispecific antibody (BsAb) to the high-affinity Fc receptor for IgG (Fc gamma RI) and to human red blood group antigen RhD were studied in vitro, using human mononuclear leucocytes as effector cells. The results w...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1992.tb06951.x

    authors： Deramoudt EX,Gilard C,Lepine N,Alonso JM,Romet-Lemonne JL

    更新日期：1992-08-01 00:00:00

  • Two novel cationic staphylococcal proteins induce IL-2 secretion, proliferation and immunoglobulin synthesis in peripheral blood mononuclear cells (PBMC) of both healthy controls and patients with common variable immunodeficiency (CVID).

    abstract：:Two cationic proteins, a neutral phosphatase (NP-tase) and a 70-kD protein (p70) were isolated from Staphylococcus aureus by ion exchange chromatography. We compared their properties to those of the well established B cell mitogen of whole, fixed Staph. aureus strain Cowan I cells (SAC). Both purified proteins were ab...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1995.tb03714.x

    authors： Jahreis A,Yousif Y,Rump JA,Dräger R,Vogt A,Peter HH,Schlesier M

    更新日期：1995-06-01 00:00:00

  • T cell activation, apoptosis and cytokine dysregulation in the (co)pathogenesis of HIV and pulmonary tuberculosis (TB).

    abstract：:Immune parameters were compared in four groups of Ugandan subjects: HIV-and HIV+ adult patients with active pulmonary TB (HIV- PTB n = 38; HIV+ PTB n = 28), patients with HIV infection only (n = 26) and PPD+ healthy controls (n = 25). Compared with healthy controls, CD4 and CD8 T cells from patients with HIV and/or PT...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2000.01385.x

    authors： Hertoghe T,Wajja A,Ntambi L,Okwera A,Aziz MA,Hirsch C,Johnson J,Toossi Z,Mugerwa R,Mugyenyi P,Colebunders R,Ellner J,Vanham G

    更新日期：2000-12-01 00:00:00

  • Antibodies against cytomegalovirus-induced early antigens (CMV-EA) in immunosuppressed renal-allograft recipients.

    abstract：:Antibodies against cytomegalovirus-specific early antigens (CMV-EA) were followed in sera, obtained from fifteen immunosuppressed renal-allograft recipients. Eight patients (sixty-two sera) showed seroconversion 47--137 days post-transplantation. Five patients (forty sera) with CMV antibodies at the moment of renal im...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： The TH,Andersen HK,Spencer ES,Klein G

    更新日期：1977-06-01 00:00:00

  • Characterization of hepatitis B surface antigen (HBsAg) induced interleukin-2 secretion in chronic asymptomatic carriers of HBsAg.

    abstract：:Peripheral T lymphocytes from asymptomatic HBsAg carriers and hepatitis B (HB) immune (naturally acquired or vaccine induced) donors were induced into interleukin-2 (IL-2) secretion by plasma-derived HBsAg and the translational products of the S- and pre-S2+S gene of HB virus (HBV) in vitro. Biphasic time courses of I...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Sylvan SP,Hekkström UB

    更新日期：1989-11-01 00:00:00

  • Relationship of CD146 expression to secretion of interleukin (IL)-17, IL-22 and interferon-γ by CD4(+) T cells in patients with inflammatory arthritis.

    abstract：:Expression of the adhesion molecule, CD146/MCAM/MelCAM, on T cells has been associated with recent activation, memory subsets and T helper type 17 (Th17) effector function, and is elevated in inflammatory arthritis. Th17 cells have been implicated in the pathogenesis of rheumatoid arthritis (RA) and spondyloarthritide...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12434

    authors： Wu C,Goodall JC,Busch R,Gaston JS

    更新日期：2015-03-01 00:00:00

  • Heterogeneity of stem cells in severe combined immunodeficiency.

    abstract：:Two patients with severe combined immunodeficiency disease (SCID) having variable B-cell development have been shown to have marrow precursors of lymphoid cells which can be induced in vitro by thymic factors to express certain T-cell surface characteristics (HTLA+ phenotypes). Their marrow cells could not, however, b...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Incefy GS,Grimes E,Kagan WA,Goldstein G,Smithwick E,O'Reilly R,Good RA

    更新日期：1976-09-01 00:00:00

  • Down-regulation of the T cell receptor CD3 zeta chain in rheumatoid arthritis (RA) and its influence on T cell responsiveness.

    abstract：:T cells implicated in chronic inflammatory diseases such as RA respond weakly when stimulated in vitro with mitogen or antigen. The mechanism behind this hyporesponsiveness is unclear, but a depressed expression of the T cell receptor (TCR)-associated CD3zeta chain has been suggested. In the present work we describe a...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2000.01180.x

    authors： Berg L,Rönnelid J,Klareskog L,Bucht A

    更新日期：2000-04-01 00:00:00

  • Demonstration of two disease specific antigens in circulating immune complexes.

    abstract：:A method is described to assess antigenic cross-reactivity between soluble immune complexes precipitated from sera with polyethylene glycol. The precipitated complex from one serum was dissociated in acid and used to coat a plastic cup. Radioiodinated complexes from another serum were dissociated in the cup, neutraliz...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Dambuyant C,Burton-Kee J,Mowbray JF

    更新日期：1979-09-01 00:00:00

  • Immunology of membranous nephropathy: from animal models to humans.

    abstract：:Membranous nephropathy (MN), the leading cause of nephrotic syndrome in adults, is characterized by the deposition of subepithelial immune deposits that consist mainly of immunoglobulin (Ig)G and complement. Most of the cases are primary or idiopathic (iMN), while only approximately 25% of the cases are secondary to s...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1111/cei.12729

    authors： Sinico RA,Mezzina N,Trezzi B,Ghiggeri GM,Radice A

    更新日期：2016-02-01 00:00:00

  • Evidence for immunological priming and increased frequency of CD4+ CD25+ cord blood T cells in children born to mothers with type 1 diabetes.

    abstract：:Maternal transmission of islet autoantibodies to children born to mothers with type 1 diabetes (T1D) has been shown to protect from autoantibodies and diabetes development later in life. However, the factors conferring disease protection are poorly understood. The aim of this study was to evaluate comparatively proinf...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,多中心研究

    doi：10.1111/j.1365-2249.2006.03243.x

    authors： Holm BC,Svensson J,Akesson C,Arvastsson J,Ljungberg J,Lynch K,Ivarsson SA,Lernmark A,Cilio CM,Diabetes Prediction Study in Skåne (DiPiS).

    更新日期：2006-12-01 00:00:00

  • Experimental autoimmune thyroiditis: attempt to correlate disease development with lymphocyte subset changes.

    abstract：:An attempt was made to correlate lymphocyte subset abnormalities with the development of experimental autoimmune thyroiditis (EAIT) in thymectomized and irradiated rats of five inbred strains. The peripheral blood subpopulations were analysed using the fluorescence-activated cell sorter (FACS-IV). A significant lympho...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Smith E,Behan WM,Menzies CB,Behan PO

    更新日期：1987-02-01 00:00:00

  • Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.

    abstract：:The efficacy of steroids and immunosuppressive treatments in idiopathic nephrotic syndrome (INS) hints at the implication of immune cells in the pathophysiology of the disease. Toll-like receptor (TLR) dysfunctions are involved in many kidney diseases of immune origin, but remain little described in INS. We investigat...

    journal_title：Clinical and experimental immunology

    pub_type： 临床试验,杂志文章

    doi：10.1111/cei.12659

    authors： Jamin A,Dehoux L,Dossier C,Fila M,Heming N,Monteiro RC,Deschênes G

    更新日期：2015-12-01 00:00:00

  • Suppression of delayed hypersensitivity skin reactions to tuberculin by M. leprae antigens in patients with lepromatous and tuberculoid leprosy.

    abstract：:Delayed hypersensitivity skin reactions to tuberculin when injected alone or in mixture with antigens of M. leprae were examined in leprosy patients and in healthy controls. The tuberculin reaction was significantly inhibited in more than one half of both LL and BT patients by the soluble extract of M. leprae (leprosi...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Sengupta U,Sinha S,Ramu G,Lamb J,Ivanyi J

    更新日期：1987-04-01 00:00:00

  • Haemorrhage-induced alterations in function and cytokine production of T cells and T cell subpopulations.

    abstract：:Haemorrhage produces alterations in macrophage, T and B cell function. In order to better define the mechanism for the effects of blood loss on immune response, we examined function of and cytokine production by purified T cells, CD4+ and CD8+ subpopulations after blood loss. Whereas T and CD4+ cells from control, unh...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1992.tb05873.x

    authors： Abraham E,Chang YH

    更新日期：1992-12-01 00:00:00

  • Cellular autoreactivity against heat shock protein 60 in renal transplant patients: peripheral and graft-infiltrating responses.

    abstract：:Autoreactivity to heat shock protein 60 (Hsp60) has been implicated in the pathogenesis and regulation of chronic inflammation, especially in autoimmune diseases. In transplantation, there is a lack of information regarding the cytokine profile and specificity of cells that recognize self-Hsp60 as well as the kinetics...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03195.x

    authors： Caldas C,Luna E,Spadafora-Ferreira M,Porto G,Iwai LK,Oshiro SE,Monteiro SM,Fonseca JA,Lemos F,Hammer J,Ho PL,Kalil J,Coelho V

    更新日期：2006-10-01 00:00:00

  • CD4+ T cells from HIV-1-infected patients recognize wild-type and mutant human immunodeficiency virus-1 protease epitopes.

    abstract：:Human immunodeficiency virus (HIV)-1 protease is a known target of CD8+ T cell responses, but it is the only HIV-1 protein in which no fully characterized HIV-1 protease CD4 epitopes have been identified to date. We investigated the recognition of HIV-1 protease by CD4+ T cells from 75 HIV-1-infected, protease inhibit...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2011.04319.x

    authors： Muller NG,Alencar R,Jamal L,Hammer J,Sidney J,Sette A,Brindeiro RM,Kalil J,Cunha-Neto E,Moraes SL

    更新日期：2011-04-01 00:00:00

  • Induction of β-defensins by l-isoleucine as novel immunotherapy in experimental murine tuberculosis.

    abstract：:Tuberculosis is a worldwide health problem, and multidrug-resistant (MDR) and extensively multidrug-resistant (XMDR) strains are rapidly emerging and threatening the control of this disease. These problems motivate the search for new treatment strategies. One potential strategy is immunotherapy using cationic anti-mic...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2010.04313.x

    authors： Rivas-Santiago CE,Rivas-Santiago B,León DA,Castañeda-Delgado J,Hernández Pando R

    更新日期：2011-04-01 00:00:00

  • Factors affecting the differential counting of human lymphocyte subpopulations in blood smears.

    abstract：:We have previously used the antibody-mediated binding of bacteria to identify Ig-bearing cells and the natural binding of bacteria to identify several lymphocyte subpopulations. By using bacteria this identification can be carried out in conventional blood smears since bacteria are small and easily distinguished from ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Bratescu A,Mayer E,Teodorescu M

    更新日期：1980-09-01 00:00:00

  • Dichotomy of the human T cell response to Leishmania antigens. II. Absent or Th2-like response to gp63 and Th1-like response to lipophosphoglycan-associated protein in cells from cured visceral leishmaniasis patients.

    abstract：:The T cell response to different Leishmania donovani antigens was investigated using peripheral blood mononuclear cells (PBMC) from Kenyans cured of visceral leishmaniasis and non-exposed Danes. Crude promastigote and amastigote antigens both induced proliferation and interferon-gamma (IFN-gamma) production in PBMC fr...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1994.tb06044.x

    authors： Kurtzhals JA,Hey AS,Jardim A,Kemp M,Schaefer KU,Odera EO,Christensen CB,Githure JI,Olafson RW,Theander TG

    更新日期：1994-06-01 00:00:00

  • A novel point mutation in CD18 causing the expression of dysfunctional CD11/CD18 leucocyte integrins in a patient with leucocyte adhesion deficiency (LAD).

    abstract：:Leucocyte adhesion deficiency type 1 (LAD-1) is characterized by the incapacity of leucocytes to carry out their adhesion functions via their CD11/CD18 antigens, which are also referred to as the leucocyte integrins. The patients generally suffer from poor wound healing and recurrent bacterial and fungal infections. I...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2000.01277.x

    authors： Mathew EC,Shaw JM,Bonilla FA,Law SK,Wright DA

    更新日期：2000-07-01 00:00:00

  • Unresponsiveness to Con A in spleen cell cultures of M. lepraemurium-infected mice is dependent on a defective expression of high-affinity IL-2 receptors rather than on a lack of IL-2 production.

    abstract：:The production of interleukin-2 (IL-2) by Con A-activated spleen cells (SC) progressively declined and reached negligible values during the course of infection of C57BL/6 mice with Mycobacterium lepraemurium. In addition, the capacity of cultured SC to utilize IL-2 was highly reduced, as demonstrated by the accumulati...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Turcotte R,Lemieux S

    更新日期：1989-04-01 00:00:00

  • Increase of circulating gamma/delta T lymphocytes in the peripheral blood of patients affected by active inflammatory bowel disease.

    abstract：:In order to study the role of gamma/delta T cells in the pathogenesis of inflammatory bowel disease (IBD) in humans, we measured the percentage of these cells in the peripheral blood, assessed the ratio of the non-disulphide-linked (delta TCS1) type of T cell receptor (TCR) in the total gamma/delta T cells, studied th...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1994.tb06611.x

    authors： Giacomelli R,Parzanese I,Frieri G,Passacantando A,Pizzuto F,Pimpo T,Cipriani P,Viscido A,Caprilli R,Tonietti G

    更新日期：1994-10-01 00:00:00

  • B7/BB1 provides an important costimulatory signal for CD3-mediated T lymphocyte proliferation in patients with systemic lupus erythematosus (SLE).

    abstract：:Successful T cell activation via the T cell receptor (TCR)/CD3 complex requires at least one contact-dependent second signal delivered by costimulatory molecules, including the B7/BB1 molecule, that are present on antigen-presenting cells (APC). SLE is characterized by multiple complex lymphocyte abnormalities of unde...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1994.tb06221.x

    authors： Sfikakis PP,Oglesby R,Sfikakis P,Tsokos GC

    更新日期：1994-04-01 00:00:00

  • Activation of c-Jun N-terminal kinase (JNK) signalling in experimentally induced gastric lesions in rats.

    abstract：:The c-Jun N-terminal kinase (JNK) participates in intracellular signalling cascades that mediate inflammatory responses. Therefore, the JNK signalling may be involved in gastric injury and inhibition of this pathway may form the basis of a new strategy for the treatment of gastric injury. The aim of this study was to ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2005.02959.x

    authors： Mitsuyama K,Tsuruta O,Matsui Y,Harada K,Tomiyasu N,Suzuki A,Takaki K,Masuda J,Handa K,Satoh Y,Bennett BL,Toyonaga A,Sata M

    更新日期：2006-01-01 00:00:00

  • The use of C1q, conglutinin and low affinity rabbit IgM antibody to human Fc in a ligand coctail radioassay for detecting and characterizing immune complexes in pathological sera.

    abstract：:A ligand radioassay for the detection of IC which utilizes C1q, bovine conglutinin and low affinity rabbit IgM anti-human Fc in a reagent coctail, is presented. IC are first isolated from serum by precipitation in polyethylene glycol, then analysed for their ability to react with the ligand coctail. Dual-label studies...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Harkiss GD,Brown DL

    更新日期：1980-03-01 00:00:00

  • Ethyl pyruvate significantly inhibits tumour necrosis factor-α, interleukin-1β and high mobility group box 1 releasing and attenuates sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury.

    abstract：:In this study, we examined the effect of ethyl pyruvate (EP) on pulmonary inflammation in rats with severe pancreatitis-associated acute lung injury (ALI). Severe acute pancreatitis (SAP) was induced in rats by the retrograde injection of 5% sodium taurocholate into the pancreatic duct. Rats were randomly divided into...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12062

    authors： Luan ZG,Zhang J,Yin XH,Ma XC,Guo RX

    更新日期：2013-06-01 00:00:00

  • PERB11 (MIC): a polymorphic MHC gene is expressed in skin and single nucleotide polymorphisms are associated with psoriasis.

    abstract：:The susceptibility genes for psoriasis remain to be identified. At least one of these must be in the major histocompatibility complex (MHC) to explain associations with alleles at human leucocyte antigen (HLA)-A, -B, -C, -DR, -DQ and C4. In fact, most of these alleles are components of just two ancestral haplotypes (A...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2000.01140.x

    authors： Tay GK,Hui J,Gaudieri S,Schmitt-Egenolf M,Martinez OP,Leelayuwat C,Williamson JF,Eiermann TH,Dawkins RL

    更新日期：2000-03-01 00:00:00